{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:25:30Z","timestamp":1791228330720,"version":"4.1.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2024,7]]},"DOI":"10.1016\/j.aca.2024.342755","type":"journal-article","created":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T06:26:30Z","timestamp":1716272790000},"page":"342755","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"special_numbering":"C","title":["Thermostability-assisted limited proteolysis-coupled mass spectrometry for capturing drug target proteins and sites"],"prefix":"10.1016","volume":"1312","author":[{"given":"Liu","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Wan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3949-9083","authenticated-orcid":false,"given":"Qi-Ming","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi-Fan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7714-203X","authenticated-orcid":false,"given":"Yasushi","family":"Ishihama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5087-8351","authenticated-orcid":false,"given":"Wen","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2024.342755_bib1","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/nchembio.1199","article-title":"Target identification and mechanism of action in chemical biology and drug discovery","volume":"9","author":"Schenone","year":"2013","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.aca.2024.342755_bib2","doi-asserted-by":"crossref","first-page":"2744","DOI":"10.1002\/anie.201208749","article-title":"Target identification for small bioactive molecules: finding the needle in the haystack","volume":"52","author":"Ziegler","year":"2013","journal-title":"Angew. Chem., Int. Ed. Engl."},{"key":"10.1016\/j.aca.2024.342755_bib3","doi-asserted-by":"crossref","first-page":"21984","DOI":"10.1073\/pnas.0910040106","article-title":"Target identification using drug affinity responsive target stability (DARTS)","volume":"106","author":"Lomenick","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.aca.2024.342755_bib4","article-title":"Tracking cancer drugs in living cells by thermal profiling of the proteome","volume":"246","author":"Savitski","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.aca.2024.342755_bib5","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1233606","article-title":"Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay","volume":"341","author":"Martinez Molina","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.aca.2024.342755_bib6","doi-asserted-by":"crossref","first-page":"4948","DOI":"10.1021\/pr200403c","article-title":"Thermodynamic analysis of protein-ligand interactions in complex biological mixtures using a shotgun proteomics approach","volume":"10","author":"Dearmond","year":"2011","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.aca.2024.342755_bib7","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1146\/annurev.biochem.75.101304.124125","article-title":"Activity-based protein profiling: from enzyme chemistry to proteomic chemistry","volume":"77","author":"Cravatt","year":"2008","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.aca.2024.342755_bib8","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.cell.2017.12.006","article-title":"A map of protein-metabolite interactions reveals principles of chemical communication","volume":"172","author":"Piazza","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.aca.2024.342755_bib9","doi-asserted-by":"crossref","first-page":"4200","DOI":"10.1038\/s41467-020-18071-x","article-title":"A machine learning-based chemoproteomic approach to identify drug targets and binding sites in complex proteomes","volume":"11","author":"Piazza","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2024.342755_bib10","doi-asserted-by":"crossref","DOI":"10.1126\/science.aai7825","article-title":"Cell-wide analysis of protein thermal unfolding reveals determinants of thermostability","volume":"355","author":"Leuenberger","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.aca.2024.342755_bib11","doi-asserted-by":"crossref","first-page":"14039","DOI":"10.1021\/acs.analchem.8b04122","article-title":"Chemo-selection strategy for limited proteolysis experiments on the proteomic scale","volume":"90","author":"Ma","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2024.342755_bib12","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1021\/acs.analchem.9b04531","article-title":"Solvent-induced protein precipitation for drug target discovery on the proteomic scale","volume":"90","author":"Zhang","year":"2020","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2024.342755_bib13","doi-asserted-by":"crossref","first-page":"13912","DOI":"10.1021\/acs.analchem.0c02756","article-title":"Microparticle-assisted precipitation screening method for robust drug target identification","volume":"92","author":"Lyu","year":"2020","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2024.342755_bib14","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/s41522-018-0075-0","article-title":"Peptidyl-prolyl isomerase-B is involved in Mycobacterium tuberculosis biofilm formation and a generic target for drug repurposing-based intervention","volume":"5","author":"Kumar","year":"2019","journal-title":"NPJ. Biofilms. Microbiomes."},{"key":"10.1016\/j.aca.2024.342755_bib15","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1016\/j.jmb.2007.02.091","article-title":"Energetics-based protein profiling on a proteomic scale: identification of proteins resistant to proteolysis","volume":"368","author":"Park","year":"2007","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.aca.2024.342755_bib16","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1038\/s41467-019-09107-y","article-title":"Proteome-wide solubility and thermal stability profiling reveals distinct regulatory roles for ATP","volume":"10","author":"Sridharan","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2024.342755_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2023.100560","article-title":"Investigation of cellular response to the HSP90 inhibition in human cells through thermal proteome profiling","volume":"22","author":"Yin","year":"2023","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.aca.2024.342755_bib18","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1021\/acschembio.1c00936","article-title":"Precipitate-supported thermal proteome profiling coupled with deep learning for comprehensive screening of drug target proteins","volume":"17","author":"Ruan","year":"2022","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.aca.2024.342755_bib19","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.201900372","article-title":"A simplified thermal proteome profiling approach to screen protein targets of a ligand","author":"Zhang","year":"2020","journal-title":"Proteomics"},{"key":"10.1016\/j.aca.2024.342755_bib20","doi-asserted-by":"crossref","first-page":"12403","DOI":"10.1039\/D2SC03326G","article-title":"Highly effective identification of drug targets at the proteome level by pH-dependent protein precipitation","volume":"13","author":"Zhang","year":"2022","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2024.342755_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2021.338612","article-title":"Mechanical stress induced protein precipitation method for drug target screening","volume":"1168","author":"Lyu","year":"2021","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2024.342755_bib23","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.chembiol.2023.02.003","article-title":"Ainsliadimer A induces ROS-mediated apoptosis in colorectal cancer cells via directly targeting peroxiredoxin 1 and 2","volume":"30","author":"Lv","year":"2023","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.aca.2024.342755_bib24","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.201900372","article-title":"A simplified thermal proteome profiling approach to screen protein targets of a ligand","author":"Zhang","year":"2020","journal-title":"Proteomics"},{"key":"10.1016\/j.aca.2024.342755_bib25","first-page":"13","article-title":"Thermal proteome profiling: unbiased assessment of protein state through heat-induced stability changes, Proteome","volume":"15","author":"Mateus","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.aca.2024.342755_bib26","doi-asserted-by":"crossref","first-page":"26879","DOI":"10.1074\/jbc.M113.492280","article-title":"Destabilization of the epidermal growth factor receptor (EGFR) by a peptide that inhibits EGFR binding to heat shock protein 90 and receptor dimerization","volume":"288","author":"Ahsan","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.aca.2024.342755_bib22","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1016\/j.freeradbiomed.2010.03.001","article-title":"Reactive oxygen species mediate hepatotoxicity induced by the Hsp90 inhibitor geldanamycin and its analogs","volume":"48","author":"Samuni","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.aca.2024.342755_bib27","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1006\/phrs.2000.0700","article-title":"Molecular mechanism of staurosporine-induced apoptosis in osteoblasts","volume":"42","author":"Chae","year":"2000","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.aca.2024.342755_bib28","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1002\/med.21762","article-title":"Tripterygium wilfordii: an inspiring resource for rheumatoid arthritis treatment","volume":"41","author":"Zhang","year":"2021","journal-title":"Med. Res. Rev."},{"key":"10.1016\/j.aca.2024.342755_bib29","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1074\/mcp.RA118.000817","article-title":"Celastrol protects from cholestatic liver injury through modulation of SIRT1-FXR signaling","volume":"18","author":"Zhao","year":"2019","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.aca.2024.342755_bib30","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/s41392-022-01231-4","article-title":"Celastrol suppresses colorectal cancer via covalent targeting peroxiredoxin 1","volume":"8","author":"Xu","year":"2023","journal-title":"Signal Transduct. Targeted Ther."},{"key":"10.1016\/j.aca.2024.342755_bib31","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1186\/s13046-019-1173-4","article-title":"Celastrol mediates autophagy and apoptosis via the ROS\/JNK and Akt\/mTOR signaling pathways in glioma cells","volume":"38","author":"Liu","year":"2019","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"10.1016\/j.aca.2024.342755_bib32","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3390\/pharmaceutics15010048","article-title":"Targeting OPA1-mediated mitochondrial fusion contributed to celastrol's anti-tumor angiogenesis effect","volume":"15","author":"Li","year":"2022","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.aca.2024.342755_bib33","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.canlet.2021.08.030","article-title":"Celastrol in cancer therapy: recent developments, challenges and prospects","volume":"521","author":"Lim","year":"2021","journal-title":"Cancer Lett."},{"key":"10.1016\/j.aca.2024.342755_bib34","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1038\/s41401-023-01067-w","article-title":"Celastrol attenuates hepatitis C virus translation and inflammatory response in mice by suppressing heat shock protein 90\u03b2","volume":"44","author":"Chen","year":"2023","journal-title":"Acta Pharmacol. Sin."},{"key":"10.1016\/j.aca.2024.342755_bib35","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.canlet.2019.12.010","article-title":"ATP citrate lyase: a central metabolic enzyme in cancer","volume":"471","author":"Icard","year":"2020","journal-title":"Cancer Lett."},{"key":"10.1016\/j.aca.2024.342755_bib36","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.cmet.2022.05.004","article-title":"Inhibition of ATP-citrate lyase improves NASH, liver fibrosis, and dyslipidemia","volume":"34","author":"Morrow","year":"2022","journal-title":"Cell Metabol."},{"key":"10.1016\/j.aca.2024.342755_bib37","first-page":"622","article-title":"UBA1: at the crossroads of ubiquitin homeostasis and neurodegeneration, trends","volume":"21","author":"Groen","year":"2015","journal-title":"Mol. Med."},{"key":"10.1016\/j.aca.2024.342755_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1124\/pharmrev.120.000053","article-title":"E1 enzymes as therapeutic targets in cancer","volume":"73","author":"Barghout","year":"2021","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.aca.2024.342755_bib39","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1093\/neuonc\/noz038","article-title":"The novel chromatin architectural regulator SND1 promotes glioma proliferation and invasion and predicts the prognosis of patients","volume":"21","author":"Yu","year":"2019","journal-title":"Neuro Oncol."},{"key":"10.1016\/j.aca.2024.342755_bib40","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.1172\/JCI90253","article-title":"Folate cycle enzyme MTHFD1L confers metabolic advantages in hepatocellular carcinoma","volume":"127","author":"Lee","year":"2017","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.aca.2024.342755_bib41","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2104759118","article-title":"Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy","volume":"118","author":"Anderson","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.aca.2024.342755_bib42","doi-asserted-by":"crossref","first-page":"8731","DOI":"10.1038\/s41598-017-09144-x","article-title":"An essential role for TAGLN2 in phagocytosis of lipopolysaccharide-activated macrophages","volume":"7","author":"Kim","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.aca.2024.342755_bib43","article-title":"Nujiangexanthone A inhibits hepatocellular carcinoma metastasis via down regulation of cofilin 1","volume":"9","author":"Zhang","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.aca.2024.342755_fur1","doi-asserted-by":"crossref","first-page":"D1211","DOI":"10.1093\/nar\/gky869","article-title":"iProX: an integrated proteome resource","volume":"47","author":"Ma","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.aca.2024.342755_fur2","doi-asserted-by":"crossref","first-page":"D1522","DOI":"10.1093\/nar\/gkab1081","article-title":"iProX in 2021: connecting proteomics data sharing with big data","volume":"50","author":"Chen","year":"2022","journal-title":"Nucleic Acids Res"}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267024005567?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267024005567?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T05:24:19Z","timestamp":1717392259000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267024005567"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":45,"alternative-id":["S0003267024005567"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2024.342755","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2024,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Thermostability-assisted limited proteolysis-coupled mass spectrometry for capturing drug target proteins and sites","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2024.342755","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"342755"}}