{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T11:26:24Z","timestamp":1793186784658,"version":"4.5.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100009104","name":"Department of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009104","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007334","name":"Ministry of Health and Family Welfare","doi-asserted-by":"publisher","award":["DHR\/YSS\/2020\/000048"],"award-info":[{"award-number":["DHR\/YSS\/2020\/000048"]}],"id":[{"id":"10.13039\/501100007334","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2024,11]]},"DOI":"10.1016\/j.aca.2024.343221","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T06:14:15Z","timestamp":1726121655000},"page":"343221","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"special_numbering":"C","title":["A rapid, specific and ultrasensitive detection of the Chikungunya virus based on RT-RPA:CRISPR\/Cas12a one-pot dual mode end-point detection system"],"prefix":"10.1016","volume":"1329","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7497-0291","authenticated-orcid":false,"given":"Pooja","family":"Bhardwaj","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahzadi","family":"Gulafshan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7597-5601","authenticated-orcid":false,"given":"Rajeev","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2024.343221_bib1","unstructured":"Chikungunya Facts, (n.d.). https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/chikungunya#:\u223c:text=Chikungunya20virus20is20transmitted20by,bite20primarily20during20daylight20hours. (accessed June 7, 2023)."},{"key":"10.1016\/j.aca.2024.343221_bib2","series-title":"Emergence of Chikungunya Virus in Indian Subcontinent after 32 Years: a Review","author":"Lahariya","year":"2006"},{"key":"10.1016\/j.aca.2024.343221_bib3","article-title":"Chikungunya epidemic: an Indian perspective","volume":"19","author":"Kalantri","year":"2006","journal-title":"Natl. Med. J. India"},{"key":"10.1016\/j.aca.2024.343221_bib4","first-page":"182","article-title":"Chikungunya fever: a threat to global public health, Pathog","volume":"112","author":"Moiz\u00e9is","year":"2018","journal-title":"Glob. Health"},{"key":"10.1016\/j.aca.2024.343221_bib5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0010152","article-title":"Diagnostic accuracy of serological tests for the diagnosis of Chikungunya virus infection: a systematic review and meta-analysis","volume":"16","author":"Andrew","year":"2022","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.aca.2024.343221_bib6","doi-asserted-by":"crossref","DOI":"10.1007\/s10096-024-04817-8","article-title":"Molecular and serological evidence of chikungunya virus infection with high case fatality among pediatric population with acute encephalitis syndrome: first report from Eastern Uttar Pradesh, India","author":"Bhardwaj","year":"2024","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.aca.2024.343221_bib7","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1080\/14737159.2021.1924059","article-title":"Molecular techniques for the genomic viral RNA detection of West Nile, Dengue, Zika and Chikungunya arboviruses: a narrative review","volume":"21","author":"Mori","year":"2021","journal-title":"Expert Rev. Mol. Diagn."},{"key":"10.1016\/j.aca.2024.343221_bib8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.4103\/0019-5359.104781","article-title":"Laboratory diagnosis of chikungunya virus: do we really need it?","volume":"65","author":"Dash","year":"2011","journal-title":"Indian J. Med. Sci."},{"issue":"2007","key":"10.1016\/j.aca.2024.343221_bib9","first-page":"764","article-title":"Chikungunya virus in US travelers returning from India","volume":"13","author":"Lanciotti","year":"2006","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.aca.2024.343221_bib10","doi-asserted-by":"crossref","DOI":"10.1111\/gtc.12797","article-title":"Development of a portable reverse transcription loop\u2010mediated isothermal amplification system to detect the E1 region of Chikungunya virus in a cost\u2010effective manner","author":"Saechue","year":"2020","journal-title":"Gene Cell."},{"key":"10.1016\/j.aca.2024.343221_bib11","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00350-19","article-title":"Beyond fever and pain: diagnostic methods for chikungunya virus","volume":"57","author":"Natrajan","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.aca.2024.343221_bib12","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1080\/15257770701845204","article-title":"Nucleic acid isothermal amplification technologies\u2014a review","volume":"27","author":"Gill","year":"2008","journal-title":"Nucleos Nucleot. Nucleic Acids"},{"key":"10.1016\/j.aca.2024.343221_bib13","doi-asserted-by":"crossref","first-page":"12491","DOI":"10.1021\/acs.chemrev.5b00428","article-title":"Isothermal amplification of nucleic acids","volume":"115","author":"Zhao","year":"2015","journal-title":"Chem. Rev."},{"key":"10.1016\/j.aca.2024.343221_bib14","doi-asserted-by":"crossref","first-page":"63e","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2024.343221_bib15","doi-asserted-by":"crossref","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLoS Biol."},{"key":"10.1016\/j.aca.2024.343221_bib16","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2020.610872","article-title":"Efficient, rapid, and sensitive detection of plant RNA viruses with one-pot RT-RPA\u2013CRISPR\/cas12a assay","volume":"11","author":"Aman","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.aca.2024.343221_bib17","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s12967-021-02741-5","article-title":"One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR\/Cas12a","volume":"19","author":"Sun","year":"2021","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.aca.2024.343221_bib18","author":"Abd"},{"key":"10.1016\/j.aca.2024.343221_bib19","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004953","article-title":"A field-deployable reverse transcription recombinase polymerase amplification assay for rapid detection of the chikungunya virus","volume":"10","author":"Patel","year":"2016","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.aca.2024.343221_bib20","article-title":"Rapid molecular detection of zika virus in acute-phase urine samples using the recombinase polymerase amplification assay","volume":"9","author":"Abd El Wahed","year":"2017","journal-title":"PLoS Curr"},{"key":"10.1016\/j.aca.2024.343221_bib21","doi-asserted-by":"crossref","DOI":"10.3389\/fchem.2021.817246","article-title":"Magnetic field-enhanced agglutination readout combined with isothermal reverse transcription recombinase polymerase amplification for rapid and sensitive molecular detection of dengue virus","volume":"9","author":"Leon","year":"2022","journal-title":"Front. Chem."},{"key":"10.1016\/j.aca.2024.343221_bib22","doi-asserted-by":"crossref","first-page":"6052","DOI":"10.3390\/ijms23116052","article-title":"Next-generation diagnostic with CRISPR\/Cas: beyond nucleic acid detection","volume":"23","author":"Bhardwaj","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.aca.2024.343221_bib23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s11032-019-1092-2","article-title":"Evaluation of CRISPR\/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in rice","volume":"40","author":"Zhang","year":"2020","journal-title":"Mol. Breed."},{"key":"10.1016\/j.aca.2024.343221_bib24","doi-asserted-by":"crossref","first-page":"829","DOI":"10.3390\/diagnostics12040829","article-title":"Rapid one-tube RPA-CRISPR\/Cas12 detection platform for methicillin-resistant Staphylococcus aureus","volume":"12","author":"Li","year":"2022","journal-title":"Diagnostics"},{"key":"10.1016\/j.aca.2024.343221_bib25","doi-asserted-by":"crossref","DOI":"10.1007\/s00253-022-12015-9","article-title":"One-pot platform for rapid detecting virus utilizing recombinase polymerase amplification and CRISPR\/Cas12a","author":"Xiong","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.aca.2024.343221_bib26","series-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.aca.2024.343221_bib27","series-title":"Curr. Top. Neglected Trop. Dis.","article-title":"Diagnosis and molecular characterization of chikungunya virus infections","author":"\u00c1lvarez-Arg\u00fcelles","year":"2019"},{"key":"10.1016\/j.aca.2024.343221_bib28","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"Mega X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.aca.2024.343221_bib29","unstructured":"Benchling, (n.d.). https:\/\/www.benchling.com\/crispr."},{"key":"10.1016\/j.aca.2024.343221_bib30","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1089\/crispr.2019.0011","article-title":"Nucleic acid detection of plant genes using CRISPR-cas13","volume":"2","author":"Abudayyeh","year":"2019","journal-title":"CRISPR J."},{"key":"10.1016\/j.aca.2024.343221_bib31","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.actatropica.2016.07.021","article-title":"Easy and inexpensive molecular detection of dengue, chikungunya and zika viruses in febrile patients","volume":"163","author":"Calvo","year":"2016","journal-title":"Acta Trop."},{"key":"10.1016\/j.aca.2024.343221_bib32","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.3390\/bios13121021","article-title":"CRISPR\/Cas12a-Based detection platform for early and rapid diagnosis of scrub typhus","volume":"13","author":"Bhardwaj","year":"2023","journal-title":"Biosensors"},{"key":"10.1016\/j.aca.2024.343221_bib33","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.aca.2024.343221_bib34","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1007\/s11274-020-02938-8","article-title":"Direct detection of methicillin-resistant in Staphylococcus spp. in positive blood culture by isothermal recombinase polymerase amplification combined with lateral flow dipstick assay","volume":"36","author":"Srisrattakarn","year":"2020","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.aca.2024.343221_bib35","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2021.800104","article-title":"iSCAN-V2: a one-pot RT-RPA\u2013CRISPR\/cas12b assay for point-of-care SARS-CoV-2 detection","volume":"9","author":"Aman","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.aca.2024.343221_bib36","article-title":"The neurological complications of chikungunya virus: a systematic review","volume":"28","author":"Mehta","year":"2018","journal-title":"Rev. Med. Virol."},{"key":"10.1016\/j.aca.2024.343221_bib37","article-title":"Development of magnetic bead based sample extraction coupled polymerase spiral reaction for rapid on-site detection of Chikungunya virus","volume":"10","author":"Sharma","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.aca.2024.343221_bib38","doi-asserted-by":"crossref","first-page":"947","DOI":"10.4269\/ajtmh.2012.11-0721","article-title":"Rapid identification of Chikungunya and Dengue virus by a real-time reverse transcription-loop-mediated isothermal amplification method","volume":"87","author":"Lu","year":"2012","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.aca.2024.343221_bib39","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/j.cmi.2024.03.004","article-title":"Development and field validation of a reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) for the rapid detection of chikungunya virus in patient and mosquito samples","volume":"30","author":"Silva","year":"2024","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.aca.2024.343221_bib40","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.ymeth.2021.02.018","article-title":"CRISPR-Cas12-based nucleic acids detection systems","volume":"203","author":"Leung","year":"2022","journal-title":"Methods"},{"key":"10.1016\/j.aca.2024.343221_bib41","doi-asserted-by":"crossref","first-page":"4906","DOI":"10.1038\/s41467-020-18615-1","article-title":"Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection","volume":"11","author":"Nguyen","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2024.343221_bib42","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1039\/D2AN01863B","article-title":"Single-tube one-step gel-based RT-RPA\/PCR for highly sensitive molecular detection of HIV","volume":"148","author":"Uno","year":"2023","journal-title":"Analyst"},{"key":"10.1016\/j.aca.2024.343221_bib43","article-title":"Virus load and clinical features during the acute phase of Chikungunya infection in children","volume":"14","author":"Siva Raghavendhar","year":"2019","journal-title":"PLoS One"}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267024010225?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267024010225?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T20:12:17Z","timestamp":1728591137000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267024010225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":43,"alternative-id":["S0003267024010225"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2024.343221","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A rapid, specific and ultrasensitive detection of the Chikungunya virus based on RT-RPA:CRISPR\/Cas12a one-pot dual mode end-point detection system","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2024.343221","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"343221"}}