{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T11:50:30Z","timestamp":1790596230209,"version":"4.1.0"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003995","name":"Anhui Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["2408085QH233"],"award-info":[{"award-number":["2408085QH233"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1016\/j.aca.2025.343703","type":"journal-article","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T19:01:00Z","timestamp":1737658860000},"page":"343703","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["A single fluorescent probe for dual-color imaging and polarity precise analysis in lipid droplets and endoplasmic reticulum"],"prefix":"10.1016","volume":"1343","author":[{"given":"Lei","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5253-1204","authenticated-orcid":false,"given":"Hui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2025.343703_bib1","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1039\/C9SC04859F","article-title":"Combining hydrophilic and hydrophobic environment sensitive dyes to detect a wide range of cellular polarity","volume":"11","author":"Park","year":"2020","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2025.343703_bib2","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abh2307","article-title":"Planar cell polarity signaling components are a direct target of \u03b2-amyloid-associated degeneration of glutamatergic synapses","volume":"7","author":"Feng","year":"2021","journal-title":"Sci. Adv."},{"key":"10.1016\/j.aca.2025.343703_bib3","article-title":"Ultrabright and highly polarity-sensitive NIR-I\/NIR-II fluorophores for the tracking of lipid droplets and staging of fatty liver disease","volume":"32","author":"Zhou","year":"2022","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.aca.2025.343703_bib4","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/nrm.2017.11","article-title":"Planar cell polarity in development and disease","volume":"18","author":"Butler","year":"2017","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.aca.2025.343703_bib5","doi-asserted-by":"crossref","first-page":"2510","DOI":"10.1002\/anie.201410645","article-title":"Ratiometric fluorescence imaging of cellular polarity: decrease in mitochondrial polarity in cancer cells","volume":"54","author":"Jiang","year":"2015","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.aca.2025.343703_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2023.133343","article-title":"Multimode evaluating the fluctuation of lipid droplets polarity in acute kidney injury and tumor models","volume":"380","author":"Pu","year":"2023","journal-title":"Sens. Actuators B Chem."},{"key":"10.1016\/j.aca.2025.343703_bib7","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1126\/science.ade2292","article-title":"CXCL9:SPP1 macrophage polarity identifies a network of cellular programs that control human cancers","volume":"381","author":"Bill","year":"2023","journal-title":"Science"},{"key":"10.1016\/j.aca.2025.343703_bib8","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.1021\/acs.chemrev.3c00573","article-title":"Design and application of fluorescent probes to detect cellular physical microenvironments","volume":"124","author":"Ma","year":"2024","journal-title":"Chem. Rev."},{"key":"10.1016\/j.aca.2025.343703_bib9","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1038\/s41566-024-01391-5","article-title":"In vivo NIR-II fluorescence imaging for biology and medicine","volume":"18","author":"Wang","year":"2024","journal-title":"Nat. Photonics"},{"key":"10.1016\/j.aca.2025.343703_bib10","doi-asserted-by":"crossref","first-page":"19478","DOI":"10.1021\/acsnano.3c03564","article-title":"Fluorescent probes for imaging in humans: where are we now","volume":"17","author":"Seah","year":"2023","journal-title":"ACS Nano"},{"key":"10.1016\/j.aca.2025.343703_bib11","doi-asserted-by":"crossref","first-page":"2533","DOI":"10.1038\/s41467-022-30153-6","article-title":"A negative-solvatochromic fluorescent probe for visualizing intracellular distributions of fatty acid metabolites","volume":"13","author":"Kajiwara","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2025.343703_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2024.342330","article-title":"Polymeric microenvironment enhancing polarity response sensitivity for discriminating lipid droplets in cancer cells","volume":"1297","author":"Zuo","year":"2024","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.343703_bib13","doi-asserted-by":"crossref","first-page":"8305","DOI":"10.1021\/acs.orglett.2c03274","article-title":"Donor-acceptor-acceptor-conjugated dual-state emissive acrylonitriles: investigating the effect of acceptor unit order and biological imaging","volume":"24","author":"Zhu","year":"2022","journal-title":"Org. Lett."},{"key":"10.1016\/j.aca.2025.343703_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115707","article-title":"Ultrabright organic fluorescent probe for quantifying the dynamics of cytosolic\/nuclear lipid droplets","volume":"241","author":"Liu","year":"2023","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2025.343703_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.ccr.2023.215407","article-title":"Advances and perspectives in fluorescent probes for imaging hepatopathy-related biomarkers","volume":"497","author":"Quan","year":"2023","journal-title":"Coord. Chem. Rev."},{"key":"10.1016\/j.aca.2025.343703_bib16","doi-asserted-by":"crossref","first-page":"2048","DOI":"10.1039\/b819316a","article-title":"Design, synthesis and biological application of chemical probes for bio-imaging","volume":"39","author":"Kikuchi","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.aca.2025.343703_bib17","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/s41377-022-00873-x","article-title":"High-fidelity carbon dots polarity probes: revealing the heterogeneity of lipids in oncology","volume":"11","author":"Hu","year":"2022","journal-title":"Light Sci. Appl."},{"key":"10.1016\/j.aca.2025.343703_bib18","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202303212","article-title":"Ketocyanine-based fluorescent probe revealing the polarity heterogeneity of lipid droplets and enabling accurate diagnosis of hepatocellular carcinoma","volume":"13","author":"He","year":"2024","journal-title":"Adv. Healthcare Mater."},{"key":"10.1016\/j.aca.2025.343703_bib19","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1083\/jcb.201401126","article-title":"Bridging the gap: membrane contact sites in signaling, metabolism, and organelle dynamics","volume":"205","author":"Prinz","year":"2014","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.aca.2025.343703_bib20","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.cell.2009.11.005","article-title":"Lipid droplets finally get a little R-E-S-P-E-C-T","volume":"139","author":"Farese","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.aca.2025.343703_bib21","doi-asserted-by":"crossref","first-page":"8875","DOI":"10.1039\/D2TB01536F","article-title":"A dual-targeting fluorescent probe for simultaneous and discriminative visualization of lipid droplets and endoplasmic reticulum","volume":"10","author":"Meng","year":"2022","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.aca.2025.343703_bib22","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.ceb.2013.02.006","article-title":"ER structure and function","volume":"25","author":"Chen","year":"2013","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.aca.2025.343703_bib23","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/nrm.2015.8","article-title":"Structure and function of ER membrane contact sites with other organelles","volume":"17","author":"Phillips","year":"2016","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.aca.2025.343703_bib24","doi-asserted-by":"crossref","first-page":"15095","DOI":"10.1002\/anie.202104163","article-title":"A polarity-sensitive ratiometric fluorescence probe for monitoring changes in lipid droplets and nucleus during ferroptosis","volume":"60","author":"Wang","year":"2021","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.aca.2025.343703_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.ccr.2020.213582","article-title":"Recent progresses in fluorescent probes for detection of polarity","volume":"427","author":"Xiao","year":"2021","journal-title":"Coord. Chem. Rev."},{"key":"10.1016\/j.aca.2025.343703_bib26","article-title":"Near-infrared-II fluorophore with inverted dependence of fluorescence quantum yield on polarity as potent phototheranostics for fluorescence-image-guided phototherapy of tumors","volume":"35","author":"Li","year":"2023","journal-title":"Adv. Mater."},{"key":"10.1016\/j.aca.2025.343703_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2022.135397","article-title":"Development of near-infrared mitochondrial polarity fluorescent probe for evaluating mitophagy in mice heart and potential cancer diagnosis","volume":"437","author":"Zhang","year":"2022","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.aca.2025.343703_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2023.134740","article-title":"A stable NIR fluorescent probe for imaging lipid droplets in triple-negative breast cancer","volume":"398","author":"Chen","year":"2024","journal-title":"Sens. Actuators B Chem."},{"key":"10.1016\/j.aca.2025.343703_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2022.123903","article-title":"An imidazole-derived polarity sensitive probe for lipid droplet target and in vivo tumor imaging","volume":"252","author":"Ma","year":"2023","journal-title":"Talanta"},{"key":"10.1016\/j.aca.2025.343703_bib30","doi-asserted-by":"crossref","first-page":"15350","DOI":"10.1021\/acs.analchem.3c03047","article-title":"Polarity-sensitive probe for two-photon fluorescence lifetime imaging of lipid droplets in vitro and in vivo","volume":"95","author":"Sha","year":"2023","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2025.343703_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.saa.2023.122973","article-title":"Development of a high polarity-sensitive fluorescent probe for visualizing the lipid droplets and endoplasmic reticulum with dual colors in living cells","volume":"301","author":"Dai","year":"2023","journal-title":"Spectrochim. Acta Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.aca.2025.343703_bib32","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1021\/acsphotonics.3c01300","article-title":"Design rules for structural colors in all-dielectric metasurfaces: from individual resonators to collective resonances and color multiplexing","volume":"11","author":"Vilayphone","year":"2024","journal-title":"ACS Photonics"},{"key":"10.1016\/j.aca.2025.343703_bib33","doi-asserted-by":"crossref","first-page":"3169","DOI":"10.1021\/jacs.0c12323","article-title":"Simultaneous two-color visualization of lipid droplets and endoplasmic reticulum and their interplay by single fluorescent probes in lambda mode","volume":"143","author":"Guo","year":"2021","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.aca.2025.343703_bib34","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1038\/nrm3699","article-title":"The biophysics and cell biology of lipid droplets","volume":"14","author":"Thiam","year":"2013","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.aca.2025.343703_bib35","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1146\/annurev-biochem-061009-102430","article-title":"Lipid droplets and cellular lipid metabolism","volume":"81","author":"Walther","year":"2012","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.aca.2025.343703_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114618","article-title":"Polarity-sensitive and lipid droplet-specific red emission fluorophore for identifying fatty liver of living mice through in vivo imaging","volume":"216","author":"Hu","year":"2022","journal-title":"Biosens. Bioelectron."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025000972?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025000972?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T11:48:16Z","timestamp":1762084096000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267025000972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":36,"alternative-id":["S0003267025000972"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2025.343703","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A single fluorescent probe for dual-color imaging and polarity precise analysis in lipid droplets and endoplasmic reticulum","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2025.343703","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"343703"}}