{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T18:48:22Z","timestamp":1788979702985,"version":"build-2803163510"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,2,2]],"date-time":"2025-02-02T00:00:00Z","timestamp":1738454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["202006010048"],"award-info":[{"award-number":["202006010048"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003042","name":"Else Kroner-Fresenius Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003042","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005722","name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005722","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007440","name":"Hans B\u00f6ckler Stiftung","doi-asserted-by":"publisher","award":["414094"],"award-info":[{"award-number":["414094"]}],"id":[{"id":"10.13039\/501100007440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007440","name":"Hans B\u00f6ckler Stiftung","doi-asserted-by":"publisher","award":["2022"],"award-info":[{"award-number":["2022"]}],"id":[{"id":"10.13039\/501100007440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012324","name":"University Hospitals","doi-asserted-by":"publisher","award":["2020_EKSP.119"],"award-info":[{"award-number":["2020_EKSP.119"]}],"id":[{"id":"10.13039\/100012324","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1016\/j.aca.2025.343728","type":"journal-article","created":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T18:08:02Z","timestamp":1737828482000},"page":"343728","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Comprehensive tissue homogenization and metabolite extraction for application in clinical metabolomics"],"prefix":"10.1016","volume":"1344","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7657-8042","authenticated-orcid":false,"given":"Yuntao","family":"Hao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0873-9358","authenticated-orcid":false,"given":"Jeannie","family":"Horak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zorica","family":"Stijepic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5771-7374","authenticated-orcid":false,"given":"Sultan Nilay","family":"Can","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Alexandra","family":"Wolff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5345-7165","authenticated-orcid":false,"given":"Berthold","family":"Koletzko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.aca.2025.343728_bib1","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1093\/jme\/tjaa006","article-title":"Choice of laboratory tissue homogenizers matters when recovering nucleic acid from medically important ticks","volume":"57","author":"Jones","year":"2020","journal-title":"J. Med. Entomol."},{"issue":"1","key":"10.1016\/j.aca.2025.343728_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2020.100231","article-title":"sncRiboTag-Seq: cell-type-specific RiboTag-Seq for cells in low abundance in mouse brain tissue","volume":"2","author":"Gao","year":"2021","journal-title":"STAR Protoc"},{"issue":"10","key":"10.1016\/j.aca.2025.343728_bib3","doi-asserted-by":"crossref","first-page":"2466","DOI":"10.3390\/biomedicines10102466","article-title":"Study on tissue homogenization buffer composition for brain mass spectrometry-based proteomics","volume":"10","author":"Karpinski","year":"2022","journal-title":"Biomedicines"},{"issue":"15","key":"10.1016\/j.aca.2025.343728_bib4","doi-asserted-by":"crossref","first-page":"3177","DOI":"10.1002\/jsfa.7057","article-title":"Diosgenin reorganises hyperglycaemia and distorted tissue lipid profile in high-fat diet-streptozotocin-induced diabetic rats","volume":"95","author":"Naidu","year":"2015","journal-title":"J. Sci. Food Agric."},{"key":"10.1016\/j.aca.2025.343728_bib5","series-title":"Biochemistry","first-page":"851","article-title":"Each organ has a unique metabolic profile","author":"Berg","year":"2006"},{"issue":"1","key":"10.1016\/j.aca.2025.343728_bib6","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.talanta.2005.02.005","article-title":"Simultaneous determination of sulfamonomethoxine, sulfadimethoxine, and their hydroxy\/N 4-acetyl metabolites with gradient liquid chromatography in chicken plasma, tissues, and eggs","volume":"67","author":"Kishida","year":"2005","journal-title":"Talanta"},{"issue":"15","key":"10.1016\/j.aca.2025.343728_bib7","doi-asserted-by":"crossref","first-page":"5781","DOI":"10.1021\/acs.analchem.3c04353","article-title":"Comprehensive metabolomic analysis of human heart tissue enabled by parallel metabolite extraction and high-resolution mass spectrometry","volume":"96","author":"Wancewicz","year":"2024","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2025.343728_bib8","first-page":"690","article-title":"Set screw homogenization of murine ocular tissue, including the whole eye","volume":"24","author":"Gooding","year":"2018","journal-title":"Mol. Vision"},{"issue":"6","key":"10.1016\/j.aca.2025.343728_bib9","doi-asserted-by":"crossref","first-page":"365","DOI":"10.3390\/metabo11060365","article-title":"Accurate lipid quantification of tissue homogenates requires suitable sample concentration, solvent composition, and homogenization procedure-a case study in murine liver","volume":"11","author":"Hoering","year":"2021","journal-title":"Metabolites"},{"key":"10.1016\/j.aca.2025.343728_bib10","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2022.932261","article-title":"Comparison of extraction methods for intracellular metabolomics of human tissues","volume":"9","author":"Andresen","year":"2022","journal-title":"Front. Mol. Biosci."},{"issue":"3","key":"10.1016\/j.aca.2025.343728_bib11","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.diagmicrobio.2015.03.018","article-title":"Improved microbiological diagnostic due to utilization of a high-throughput homogenizer for routine tissue processing","volume":"82","author":"Redanz","year":"2015","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.aca.2025.343728_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2024.342758","article-title":"Exploring sample treatment strategies for untargeted metabolomics: a comparative study of solid phase microextraction (SPME) and homogenization with solid-liquid extraction (SLE) in renal tissue","volume":"1312","author":"Cuevas-Delgado","year":"2024","journal-title":"Anal. Chim. Acta"},{"issue":"5","key":"10.1016\/j.aca.2025.343728_bib13","doi-asserted-by":"crossref","first-page":"453","DOI":"10.3390\/metabo12050453","article-title":"A comparison of solvent-based extraction methods to assess the central carbon metabolites in mouse bone and muscle","volume":"12","author":"Dias","year":"2022","journal-title":"Metabolites"},{"issue":"194","key":"10.1016\/j.aca.2025.343728_bib14","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1126\/scitranslmed.3005623","article-title":"Intraoperative tissue identification using rapid evaporative ionization mass spectrometry","volume":"5","author":"Balog","year":"2013","journal-title":"Sci. Transl. Med."},{"issue":"14","key":"10.1016\/j.aca.2025.343728_bib15","doi-asserted-by":"crossref","first-page":"5906","DOI":"10.1021\/acs.analchem.1c00270","article-title":"Validation of ultrasonic harmonic scalpel for real-time tissue identification using rapid evaporative ionization mass spectrometry","volume":"93","author":"Manoli","year":"2021","journal-title":"Anal. Chem. (Washington, DC, U. S.)"},{"issue":"4","key":"10.1016\/j.aca.2025.343728_bib16","first-page":"493","article-title":"Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues","volume":"7","author":"Stoeckli","year":"2001","journal-title":"Nat. Med. (N. Y., NY, U. S.)"},{"issue":"1","key":"10.1016\/j.aca.2025.343728_bib17","doi-asserted-by":"crossref","first-page":"2692","DOI":"10.1038\/s41467-023-38360-5","article-title":"Spatially resolved multi-omics highlights cell-specific metabolic remodeling and interactions in gastric cancer","volume":"14","author":"Sun","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2025.343728_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2023.464349","article-title":"Automated sample preparation with 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate and iodoacetamide derivatization reagents for enantioselective liquid chromatography tandem mass spectrometry amino acid analysis","volume":"1708","author":"Li","year":"2023","journal-title":"J. Chromatogr. A"},{"issue":"8","key":"10.1016\/j.aca.2025.343728_bib19","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/o59-099","article-title":"A rapid method of total lipid extraction and purification. FIELD Authors:BLIGH, E G; DYER, W J","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"10.1016\/j.aca.2025.343728_bib20","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.aca.2018.10.035","article-title":"Comparison of simple monophasic versus classical biphasic extraction protocols for comprehensive UHPLC-MS\/MS lipidomic analysis of Hela cells","volume":"1048","author":"Calderon","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.343728_bib21","doi-asserted-by":"crossref","DOI":"10.3389\/fchem.2022.869732","article-title":"Deep metabolic profiling assessment of tissue extraction protocols for three model organisms","volume":"10","author":"Gegner","year":"2022","journal-title":"Front. Chem."},{"issue":"1","key":"10.1016\/j.aca.2025.343728_bib22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11306-017-1312-x","article-title":"High-throughput extraction and quantification method for targeted metabolomics in murine tissues","volume":"14","author":"Zukunft","year":"2018","journal-title":"Metabolomics"},{"key":"10.1016\/j.aca.2025.343728_bib23","article-title":"Comparison of one-phase and two-phase extraction methods for porcine tissue lipidomics applying a fast and reliable tentative annotation workflow","volume":"236","author":"Boegl","year":"2022","journal-title":"Talanta"},{"issue":"4","key":"10.1016\/j.aca.2025.343728_bib24","doi-asserted-by":"crossref","first-page":"504","DOI":"10.3390\/metabo13040504","article-title":"Effects of different storage conditions on lipid stability in mice tissue homogenates","volume":"13","author":"Dorochow","year":"2023","journal-title":"Metabolites"},{"issue":"1","key":"10.1016\/j.aca.2025.343728_bib25","first-page":"55","article-title":"Solid-phase microextraction of endogenous metabolites from intact tissue validated using a Biocrates standard reference method kit","volume":"13","author":"Jiang","year":"2023","journal-title":"FIELD Authors:Jiang, Runshan Will; Jaroch, Karol; Pawliszyn, Janusz, J Pharm Anal"},{"issue":"2","key":"10.1016\/j.aca.2025.343728_bib26","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1309\/3WM7XJ7RD8BCLNKX","article-title":"Effects of storage time and exogenous protease inhibitors on plasma protein levels","volume":"126","author":"Ayache","year":"2006","journal-title":"Am. J. Clin. Pathol."},{"issue":"1","key":"10.1016\/j.aca.2025.343728_bib27","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1021\/acsmeasuresciau.1c00007","article-title":"A workflow to perform targeted metabolomics at the untargeted scale on a triple quadrupole mass spectrometer","volume":"1","author":"Schwaiger-Haber","year":"2021","journal-title":"ACS Meas. Sci. Au"},{"key":"10.1016\/j.aca.2025.343728_bib28","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jmsacl.2023.02.002","article-title":"Pre-analytical sample handling standardization for reliable measurement of metabolites and lipids in LC-MS-based clinical research","volume":"28","author":"Sens","year":"2023","journal-title":"J. Mass Spectrom. Adv. Clin. Lab"},{"key":"10.1016\/j.aca.2025.343728_bib29","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.plefa.2019.09.001","article-title":"Temperature and time-dependent effects of delayed blood processing on oxylipin concentrations in human plasma, Prostaglandins, Leukotrienes Essent","volume":"150","author":"Ramsden","year":"2019","journal-title":"Fatty Acids"},{"issue":"12","key":"10.1016\/j.aca.2025.343728_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0145794","article-title":"Longitudinal metabolomic profiling of amino acids and lipids across healthy pregnancy","volume":"10","author":"Lindsay","year":"2015","journal-title":"PLoS One"},{"issue":"10","key":"10.1016\/j.aca.2025.343728_bib31","volume":"16","year":"2024","journal-title":"Pharmaceutics"},{"issue":"7\u20138","key":"10.1016\/j.aca.2025.343728_bib32","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/j.jchromb.2011.01.010","article-title":"Quantification of 22 plasma amino acids combining derivatization and ion-pair LC-MS\/MS","volume":"879","author":"Harder","year":"2011","journal-title":"J. Chromatogr., B: Anal. Technol. Biomed. Life Sci."},{"issue":"9","key":"10.1016\/j.aca.2025.343728_bib33","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.3390\/nu13093022","article-title":"Acute metabolic response in adults to toddler milk formulas with alternating higher and lower protein and fat contents, a randomized cross-over trial","volume":"13","author":"Newton-Tanzer","year":"2021","journal-title":"Nutrients"},{"issue":"10","key":"10.1016\/j.aca.2025.343728_bib34","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1194\/jlr.D061721","article-title":"An LC-MS\/MS method to quantify acylcarnitine species including isomeric and odd-numbered forms in plasma and tissues","volume":"56","author":"Giesbertz","year":"2015","journal-title":"J. Lipid Res."},{"issue":"1","key":"10.1016\/j.aca.2025.343728_bib35","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s13395-022-00312-w","article-title":"Sarcopenia: investigation of metabolic changes and its associated mechanisms","volume":"13","author":"Marques","year":"2023","journal-title":"Skeletal Muscle"},{"issue":"3","key":"10.1016\/j.aca.2025.343728_bib36","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1210\/jc.2015-3525","article-title":"Lipidomics reveals associations of phospholipids with obesity and insulin resistance in young adults","volume":"101","author":"Rauschert","year":"2016","journal-title":"J. Clin. Endocrinol. Metab."},{"issue":"18","key":"10.1016\/j.aca.2025.343728_bib37","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1093\/bioinformatics\/btx364","article-title":"UpSetR: an R package for the visualization of intersecting sets and their properties","volume":"33","author":"Conway","year":"2017","journal-title":"Bioinformatics"},{"key":"10.1016\/j.aca.2025.343728_bib38","author":"Krassowski"},{"issue":"7","key":"10.1016\/j.aca.2025.343728_bib39","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1093\/jat\/bkaa072","article-title":"Development and validation of an LC-MS-MS method for the detection of 40 benzodiazepines and three Z-drugs in blood and urine by solid-phase extraction","volume":"44","author":"Sofalvi","year":"2020","journal-title":"J. Anal. Toxicol."},{"key":"10.1016\/j.aca.2025.343728_bib40","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jmsacl.2024.02.001","article-title":"A validated liquid chromatography-tandem mass spectrometry assay for the analysis of isoniazid and its metabolite acetyl-isoniazid in urine","volume":"32","author":"Maputla","year":"2024","journal-title":"J. Mass Spectrom. Adv. Clin. Lab"},{"issue":"1","key":"10.1016\/j.aca.2025.343728_bib41","doi-asserted-by":"crossref","DOI":"10.1080\/19382014.2015.1024405","article-title":"Structural similarities and differences between the human and the mouse pancreas. FIELD authors:dolen\u0161ek, jurij; rupnik, marjan slak; sto\u017eer, andra\u017e","volume":"7","author":"Dolen\u0161ek","year":"2015","journal-title":"Islets"},{"key":"10.1016\/j.aca.2025.343728_bib42","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.trac.2013.04.010","volume":"50","author":"Ga\u0142uszka","year":"2013","journal-title":"TrAC Trends Anal. Chem."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025001229?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025001229?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T11:48:15Z","timestamp":1762084095000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267025001229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":42,"alternative-id":["S0003267025001229"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2025.343728","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comprehensive tissue homogenization and metabolite extraction for application in clinical metabolomics","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2025.343728","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"343728"}}