{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T17:36:43Z","timestamp":1778866603598,"version":"3.51.4"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173782"],"award-info":[{"award-number":["82173782"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373828"],"award-info":[{"award-number":["82373828"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.aca.2025.343935","type":"journal-article","created":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T15:30:17Z","timestamp":1741879817000},"page":"343935","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["A dual-cycle DNA walker sensor for sensitive clinical detection of microRNAs"],"prefix":"10.1016","volume":"1352","author":[{"given":"Yu","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2381-8142","authenticated-orcid":false,"given":"Xudan","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haihong","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun Zhe","family":"Min","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiabin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0876-0050","authenticated-orcid":false,"given":"Sheng","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2025.343935_bib1","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1038\/nrg1379","article-title":"Micrornas: small RNAs with a big role in gene regulation","volume":"5","author":"He","year":"2004","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.aca.2025.343935_bib2","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/nrc1840","article-title":"Oncomirs - microRNAs with a role in cancer","volume":"6","author":"Esquela-Kerscher","year":"2006","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.aca.2025.343935_bib3","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.gep.2008.10.001","article-title":"Expression of islet-specific microRNAs during human pancreatic development, Gene Expr","volume":"9","author":"Joglekar","year":"2009","journal-title":"Patterns"},{"key":"10.1016\/j.aca.2025.343935_bib4","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: target recognition and regulatory functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.aca.2025.343935_bib5","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0092-8674(04)00045-5","article-title":"MicroRNAs: genomics, biogenesis, mechanism, and function","volume":"116","author":"Bartel","year":"2004","journal-title":"Cell"},{"key":"10.1016\/j.aca.2025.343935_bib6","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.cca.2021.01.008","article-title":"Circulating microRNAs: biomarkers of disease","volume":"516","author":"Wu","year":"2021","journal-title":"Clin. Chim. Acta"},{"key":"10.1016\/j.aca.2025.343935_bib7","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1016\/j.jpha.2022.05.009","article-title":"Sensitive detection of microRNAs using polyadenine-mediated fluorescent spherical nucleic acids and a microfluidic electrokinetic signal amplification chip","volume":"12","author":"Xu","year":"2022","journal-title":"J. Pharm. Anal."},{"key":"10.1016\/j.aca.2025.343935_bib8","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1007\/s10620-016-4156-8","article-title":"Oncomirs miRNA-221\/222 and tumor suppressors miRNA-199a\/195 are crucial miRNAs in liver cancer: a systematic analysis","volume":"61","author":"Li","year":"2016","journal-title":"Dig. Dis. Sci."},{"key":"10.1016\/j.aca.2025.343935_bib9","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.coelec.2017.09.014","article-title":"Bioelectrochemistry for miRNA detection","volume":"5","author":"Ciui","year":"2017","journal-title":"Curr. Opin. Electroche."},{"key":"10.1016\/j.aca.2025.343935_bib10","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1016\/j.jpha.2023.08.009","article-title":"Application of microfluidic technology based on surface-enhanced Raman scattering in cancer biomarker detection: a review","volume":"13","author":"Nie","year":"2023","journal-title":"J. Pharm. Anal."},{"key":"10.1016\/j.aca.2025.343935_bib11","article-title":"Multiplexed miRNA northern blots via hybridization chain reaction","volume":"44","author":"Schwarzkopf","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2025.343935_bib12","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s13206-012-6104-9","article-title":"A PNA microarray platform for miRNA expression profiling using on-chip labeling technology","volume":"6","author":"Kim","year":"2012","journal-title":"BioChip J"},{"key":"10.1016\/j.aca.2025.343935_bib13","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.clinbiochem.2019.03.008","article-title":"Droplet digital PCR improves urinary exosomal miRNA detection compared to real-time PCR","volume":"67","author":"Wang","year":"2019","journal-title":"Clin. Biochem."},{"key":"10.1016\/j.aca.2025.343935_bib14","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1101\/gr.4.6.357","article-title":"Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic-acid hybridization","volume":"4","author":"Livak","year":"1995","journal-title":"PCR Meth. Appl."},{"key":"10.1016\/j.aca.2025.343935_bib15","doi-asserted-by":"crossref","first-page":"155","DOI":"10.2144\/000114002","article-title":"A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability","volume":"54","author":"Redshaw","year":"2013","journal-title":"Biotechniques"},{"key":"10.1016\/j.aca.2025.343935_bib16","doi-asserted-by":"crossref","first-page":"2150","DOI":"10.1093\/nar\/26.9.2150","article-title":"Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA","volume":"26","author":"Leone","year":"1998","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2025.343935_bib17","doi-asserted-by":"crossref","first-page":"2935","DOI":"10.1039\/c3cc49873e","article-title":"A rolling circle amplification-based DNA machine for miRNA screening coupling catalytic hairpin assembly with DNAzyme formation","volume":"50","author":"Zhuang","year":"2014","journal-title":"Chem. Commun."},{"key":"10.1016\/j.aca.2025.343935_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2019.111789","article-title":"Electrochemiluminescence biosensor for miRNA-21 based on toehold-mediated strand displacement amplification with Ru(phen)32+ loaded DNA nanoclews as signal tags","volume":"147","author":"Zhang","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2025.343935_bib19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2020.06.007","article-title":"Reverse transcription-based loop-mediated isothermal amplification strategy for real-time miRNA detection with phosphorothioated probes","volume":"1126","author":"Al-maskri","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.343935_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2023.135187","article-title":"Dual-mode biosensors for ultrasensitive detection of Legionella pneumophila using CRISPR\/Cas12a integrated recombinase polymerase amplification","volume":"403","author":"Qiu","year":"2024","journal-title":"Sens. Actuators, B"},{"key":"10.1016\/j.aca.2025.343935_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2022.339663","article-title":"Mismatch-fueled catalytic hairpin assembly mediated ultrasensitive biosensor for rapid detection of MicroRNA","volume":"1204","author":"Liu","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.343935_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2021.338930","article-title":"Applications of hybridization chain reaction optical detection incorporating nanomaterials: a review","volume":"1190","author":"Li","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.343935_bib23","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/nnano.2015.246","article-title":"A stochastic DNA walker that traverses a microparticle surface","volume":"11","author":"Jung","year":"2016","journal-title":"Nat. Nanotechnol."},{"key":"10.1016\/j.aca.2025.343935_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2020.113842","article-title":"Nanoscale assembly line composed of dual DNA-machines enabling sensitive microRNA detection using upconversion nanoparticles probes","volume":"195","author":"Xu","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.aca.2025.343935_bib25","doi-asserted-by":"crossref","first-page":"13952","DOI":"10.1021\/acs.analchem.1c03215","article-title":"Dual 3D DNA nanomachine-mediated catalytic hairpin assembly for ultrasensitive detection of MicroRNA","volume":"93","author":"Zhang","year":"2021","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2025.343935_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2021.339339","article-title":"Engineering DNA walkers for bioanalysis: a review","volume":"1209","author":"Chen","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.343935_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.microc.2022.107510","article-title":"A new strategy based on duplex-specific nuclease and DNA aptamer with modified hairpin structure for various analytes detection","volume":"179","author":"Zhong","year":"2022","journal-title":"Microchem. J."},{"key":"10.1016\/j.aca.2025.343935_bib28","doi-asserted-by":"crossref","first-page":"8820","DOI":"10.1021\/acs.analchem.8b04468","article-title":"Duplex-specific nuclease-mediated amplification strategy for mass spectrometry quantification of MiRNA-200c in breast cancer stem cells","volume":"91","author":"Kuang","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2025.343935_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2021.339182","article-title":"Target invasion-triggered signal amplification based on duplex-specific nuclease for selective and sensitive detection of miRNAs","volume":"1189","author":"Zhang","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.343935_bib30","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.aca.2020.01.041","article-title":"Duplex-specific nuclease assisted miRNA assay based on gold and silver nanoparticles co-decorated on electrode interface","volume":"1107","author":"Wang","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.343935_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2021.338937","article-title":"A mismatch-suppressed, duplex-specific nuclease powered nanowalker for multiplexed sensing of microRNA","volume":"1182","author":"Chen","year":"2021","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.343935_bib32","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.tibtech.2014.12.008","article-title":"Duplex-specific nuclease-mediated bioanalysis","volume":"33","author":"Qiu","year":"2015","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.aca.2025.343935_bib33","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1002\/bio.3677","article-title":"A conformational switch-based aptasensor for the chemiluminescence detection of microRNA","volume":"34","author":"Cai","year":"2019","journal-title":"Luminescence"},{"key":"10.1016\/j.aca.2025.343935_bib34","doi-asserted-by":"crossref","first-page":"2800","DOI":"10.1021\/acssensors.0c00747","article-title":"Chemiluminescence resonance energy transfer-based mesoporous silica nanosensors for the detection of miRNA","volume":"5","author":"Shen","year":"2020","journal-title":"ACS Sens."},{"key":"10.1016\/j.aca.2025.343935_bib35","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.jpha.2021.10.001","article-title":"Multiple rapid-responsive probes for hypochlorite detection based on dioxetane luminophore derivatives","volume":"12","author":"Sun","year":"2022","journal-title":"J. Pharm. Anal."},{"key":"10.1016\/j.aca.2025.343935_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2022.339693","article-title":"A visual method for determination of hepatitis C virus RNAs based on a 3D nanocomposite prepared from graphene quantum dots","volume":"1203","author":"Li","year":"2022","journal-title":"Anal. Chim. Acta"}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025003290?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025003290?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T11:48:31Z","timestamp":1762084111000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267025003290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":36,"alternative-id":["S0003267025003290"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2025.343935","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A dual-cycle DNA walker sensor for sensitive clinical detection of microRNAs","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2025.343935","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"343935"}}