{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:09:02Z","timestamp":1792613342104,"version":"4.5.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004374","name":"Chongqing Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004374","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.aca.2025.344389","type":"journal-article","created":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T02:42:03Z","timestamp":1751683323000},"page":"344389","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["CRISPR\/Cas13a combined with reverse transcription-multienzyme isothermal rapid amplification for hepatitis B virus RNA detection"],"prefix":"10.1016","volume":"1370","author":[{"given":"Chuiyu","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomei","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2025.344389_bib1","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/S0140-6736(22)01468-4","article-title":"Hepatitis B","volume":"401","author":"Jeng","year":"2023","journal-title":"Lancet"},{"key":"10.1016\/j.aca.2025.344389_bib2","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/S2468-1253(19)30119-0","article-title":"A global scientific strategy to cure hepatitis B","volume":"4","author":"Revill","year":"2019","journal-title":"Lancet Gastroenterol. Hepatol."},{"key":"10.1016\/j.aca.2025.344389_bib3","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1002\/hep.31026","article-title":"Pregenomic HBV RNA and Hepatitis B Core-Related Antigen Predict Outcomes in Hepatitis B e Antigen-Negative Chronic Hepatitis B Patients Suppressed on Nucleos(T)ide Analogue Therapy","volume":"72","author":"Carey","year":"2020","journal-title":"Hepatology"},{"key":"10.1016\/j.aca.2025.344389_bib4","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1002\/hep.31240","article-title":"Rapid turnover of hepatitis B virus covalently closed circular DNA indicated by monitoring emergence and reversion of signature-mutation in treated chronic hepatitis B patients","volume":"73","author":"Huang","year":"2021","journal-title":"Hepatology"},{"key":"10.1016\/j.aca.2025.344389_bib5","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1002\/hep.31616","article-title":"HBV RNA profiles in patients with chronic hepatitis B under different disease phases and antiviral therapy","volume":"73","author":"Mak","year":"2021","journal-title":"Hepatology"},{"key":"10.1016\/j.aca.2025.344389_bib6","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1093\/infdis\/jiab597","article-title":"Association of serum hepatitis B virus RNA with hepatocellular carcinoma risk in chronic hepatitis B patients under nucleos(t)ide analogues therapy","volume":"226","author":"Liu","year":"2022","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.aca.2025.344389_bib7","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1007\/s00535-021-01780-5","article-title":"Residual HBV DNA and pgRNA viraemia is associated with hepatocellular carcinoma in chronic hepatitis B patients on antiviral therapy","volume":"56","author":"Mak","year":"2021","journal-title":"J. Gastroenterol."},{"key":"10.1016\/j.aca.2025.344389_bib8","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1008945","article-title":"Biogenesis and molecular characteristics of serum hepatitis B virus RNA","volume":"16","author":"Shen","year":"2020","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.aca.2025.344389_bib9","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.jhep.2021.07.023","article-title":"A global survey of alternative splicing of HBV transcriptome using long-read sequencing","volume":"76","author":"Guan","year":"2022","journal-title":"J. Hepatol."},{"key":"10.1016\/j.aca.2025.344389_bib10","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1002\/hep.30844","article-title":"Hepatitis B virus virions produced under nucleos(t)ide analogue treatment are mainly not infectious because of irreversible DNA chain termination","volume":"71","author":"Liu","year":"2020","journal-title":"Hepatology"},{"key":"10.1016\/j.aca.2025.344389_bib11","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jhep.2020.01.028","article-title":"Full-length 5'RACE identifies all major HBV transcripts in HBV-infected hepatocytes and patient serum","volume":"73","author":"Stadelmayer","year":"2020","journal-title":"J. Hepatol."},{"key":"10.1016\/j.aca.2025.344389_bib12","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1002\/hep.27381","article-title":"Serum hepatitis B virus RNA levels as an early predictor of hepatitis B envelope antigen seroconversion during treatment with polymerase inhibitors","volume":"61","author":"van B\u00f6mmel","year":"2015","journal-title":"Hepatology"},{"key":"10.1016\/j.aca.2025.344389_bib13","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1080\/22221751.2022.2045874","article-title":"A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients","volume":"11","author":"Yu","year":"2022","journal-title":"Emerg. Microb. Infect."},{"key":"10.1016\/j.aca.2025.344389_bib14","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1136\/gutjnl-2023-330644","article-title":"Quantification of circulating HBV RNA expressed from intrahepatic cccDNA in untreated and NUC treated patients with chronic hepatitis B","volume":"73","author":"Testoni","year":"2024","journal-title":"Gut"},{"key":"10.1016\/j.aca.2025.344389_bib15","doi-asserted-by":"crossref","first-page":"3365","DOI":"10.1002\/jmv.25792","article-title":"Reverse transcriptase droplet digital PCR vs reverse transcriptase quantitative real-time PCR for serum HBV RNA quantification","volume":"92","author":"Limothai","year":"2020","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.aca.2025.344389_bib16","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jhep.2017.08.021","article-title":"Relationship between serum HBV-RNA levels and intrahepatic viral as well as histologic activity markers in entecavir-treated patients","volume":"68","author":"Wang","year":"2018","journal-title":"J. Hepatol."},{"key":"10.1016\/j.aca.2025.344389_bib17","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.jhep.2019.11.003","article-title":"Guidance for design and endpoints of clinical trials in chronic hepatitis B - Report from the 2019 EASL-AASLD HBV treatment endpoints conference(\u2021)","volume":"72","author":"Cornberg","year":"2020","journal-title":"J. Hepatol."},{"key":"10.1016\/j.aca.2025.344389_bib18","doi-asserted-by":"crossref","DOI":"10.1002\/smtd.202200794","article-title":"CRISPR\/cas systems-inspired nano\/biosensors for detecting infectious viruses and pathogenic bacteria","volume":"6","author":"Zhang","year":"2022","journal-title":"Small Methods"},{"key":"10.1016\/j.aca.2025.344389_bib19","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1016\/j.tibtech.2022.04.006","article-title":"CRISPR-Cas-mediated diagnostics","volume":"40","author":"Li","year":"2022","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.aca.2025.344389_bib20","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.biotechadv.2017.11.008","article-title":"Harnessing CRISPR\/cas systems for programmable transcriptional and post-transcriptional regulation","volume":"36","author":"Mahas","year":"2018","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.aca.2025.344389_bib21","doi-asserted-by":"crossref","DOI":"10.1126\/science.aaf5573","article-title":"C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector","volume":"353","author":"Abudayyeh","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.aca.2025.344389_bib22","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/nature19802","article-title":"Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection","volume":"538","author":"East-Seletsky","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.aca.2025.344389_bib23","doi-asserted-by":"crossref","first-page":"7733","DOI":"10.3390\/ijms24097733","article-title":"Rapid and sensitive detection of Streptococcus iniae in Trachinotus ovatus based on multienzyme isothermal rapid amplification","volume":"24","author":"Wang","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.aca.2025.344389_bib24","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1016\/j.jhep.2022.12.014","article-title":"Discovery of a first-in-class orally available HBV cccDNA inhibitor","volume":"78","author":"Wang","year":"2023","journal-title":"J. Hepatol."},{"key":"10.1016\/j.aca.2025.344389_bib25","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.jhep.2021.11.024","article-title":"How to achieve functional cure of HBV: stopping NUCs, adding interferon or new drug development?","volume":"76","author":"Wong","year":"2022","journal-title":"J. Hepatol."},{"key":"10.1016\/j.aca.2025.344389_bib26","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1136\/gutjnl-2022-328380","article-title":"Quantification of the hepatitis B virus cccDNA: evidence-based guidelines for monitoring the key obstacle of HBV cure","volume":"72","author":"Allweiss","year":"2023","journal-title":"Gut"},{"key":"10.1016\/j.aca.2025.344389_bib27","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s12985-018-0994-7","article-title":"High serum levels of pregenomic RNA reflect frequently failing reverse transcription in hepatitis B virus particles","volume":"15","author":"Prakash","year":"2018","journal-title":"Virol. J."},{"key":"10.1016\/j.aca.2025.344389_bib28","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/j.cgh.2019.07.046","article-title":"Association between negative results from tests for HBV DNA and RNA and durability of response after discontinuation of nucles(t)ide analogue therapy","volume":"18","author":"Fan","year":"2020","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"10.1016\/j.aca.2025.344389_bib29","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1016\/j.jhep.2013.06.018","article-title":"Hepatitis B virus splicing is enhanced prior to development of hepatocellular carcinoma","volume":"59","author":"Bayliss","year":"2013","journal-title":"J. Hepatol."},{"key":"10.1016\/j.aca.2025.344389_bib30","doi-asserted-by":"crossref","DOI":"10.1002\/jmv.29400","article-title":"Serum hepatitis B virus spliced RNA proportion increases with liver disease progression in patients with chronic hepatitis B","volume":"96","author":"Lu","year":"2024","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.aca.2025.344389_bib31","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.00383-24","article-title":"Rapid and sensitive detection of chikungunya virus using one-tube, reverse transcription, semi-nested multi-enzyme isothermal rapid amplification, and lateral flow dipstick assays","volume":"62","author":"Wu","year":"2024","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.aca.2025.344389_bib32","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-92329-2","article-title":"A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology","volume":"11","author":"Gong","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.aca.2025.344389_bib33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0250942","article-title":"Development of multiplex real-time RT-PCR assay for the detection of SARS-CoV-2","volume":"16","author":"Tombuloglu","year":"2021","journal-title":"PLoS One"},{"key":"10.1016\/j.aca.2025.344389_bib34","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.aaq0179","article-title":"Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.aca.2025.344389_bib35","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1038\/s42003-021-02001-8","article-title":"Amplification-free RNA detection with CRISPR-Cas13","volume":"4","author":"Shinoda","year":"2021","journal-title":"Commun. Biol."},{"key":"10.1016\/j.aca.2025.344389_bib36","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1038\/s41587-022-01213-5","article-title":"Designing sensitive viral diagnostics with machine learning","volume":"40","author":"Metsky","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.aca.2025.344389_bib37","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1093\/nar\/gkad1132","article-title":"New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity","volume":"52","author":"Molina Vargas","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2025.344389_bib38","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1111\/jvh.13926","article-title":"Biogenesis of serum HBV RNA and clinical phenomena of serum HBV RNA in chronic hepatitis B patients before and after receiving nucleos(t)ide analogues therapy","volume":"31","author":"Wu","year":"2024","journal-title":"J. Viral Hepat."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025007834?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025007834?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T19:25:30Z","timestamp":1770924330000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267025007834"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":38,"alternative-id":["S0003267025007834"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2025.344389","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"CRISPR\/Cas13a combined with reverse transcription-multienzyme isothermal rapid amplification for hepatitis B virus RNA detection","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2025.344389","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"344389"}}