{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:24:38Z","timestamp":1790083478335,"version":"4.0.1"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018556","name":"Science and Technology Program of Suzhou","doi-asserted-by":"publisher","award":["2022SS28"],"award-info":[{"award-number":["2022SS28"]}],"id":[{"id":"10.13039\/501100018556","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.aca.2025.344830","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T17:59:16Z","timestamp":1761587956000},"page":"344830","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["A novel multiplex RPA\/CRISPR-Cas12a integrated biosensor for on-site detection of high-risk HPV genotypes"],"prefix":"10.1016","volume":"1382","author":[{"given":"Yuying","family":"Bao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiting","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liguo","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kairui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanxi","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxian","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanping","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2025.344830_bib1","article-title":"Cervical cancer. World health organization","year":"2024","journal-title":"Cervical Cancer."},{"key":"10.1016\/j.aca.2025.344830_bib2","volume":"7","author":"Justiz-Vaillant","year":"2024"},{"key":"10.1016\/j.aca.2025.344830_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115323","article-title":"Cas12a\/blocker DNA-based multiplex nucleic acid detection system for diagnosis of high-risk human papillomavirus infection","volume":"232","author":"Han","year":"2023","journal-title":"Biosens. Bioelectron."},{"issue":"11","key":"10.1016\/j.aca.2025.344830_bib4","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/S1470-2045(10)70230-8","article-title":"Human papillomavirus genotype attribution in invasive cervical cancer: a retrospective cross-sectional worldwide study","volume":"11","author":"Sanjose","year":"2010","journal-title":"Lancet Oncol."},{"key":"10.1016\/j.aca.2025.344830_bib5","first-page":"17","article-title":"A cervical cancer-free future: first-ever global commitment to eliminate a cancer. A cervical cancer-free future: first-ever global commitment to eliminate a cancer","volume":"11","year":"2020"},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib6","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/s12985-019-1197-6","article-title":"CIN2+ detection of the HPV DNA array genotyping assay in comparison with the Cobas 4800 HPV test and cytology","volume":"16","author":"Pesic","year":"2019","journal-title":"Virol. J."},{"issue":"3","key":"10.1016\/j.aca.2025.344830_bib7","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.tjog.2019.03.010","article-title":"Comparison of the diagnostic value of the visual inspection with acetic acid (VIA) and Pap smear in cervical cancer screening","volume":"58","author":"Vahedpoor","year":"2019","journal-title":"Taiwan. J. Obstet. Gynecol."},{"key":"10.1016\/j.aca.2025.344830_bib8","doi-asserted-by":"crossref","DOI":"10.1136\/bmj.h2372","article-title":"HPV testing as a screen for cervical cancer","volume":"350","author":"Goodman","year":"2015","journal-title":"BMJ"},{"key":"10.1016\/j.aca.2025.344830_bib9","first-page":"43","article-title":"Analysis of TCT and HPV results in patients with cervical intraepithelial neoplasia, Chinese and foreign","volume":"7","author":"Yi","year":"2020","journal-title":"Women's Health Res."},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib10","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1309\/AJCP4Q0NSDHWIZGU","article-title":"Reliability of the Xpert HPV assay to detect high-risk human papillomavirus DNA in a colposcopy referral population","volume":"143","author":"Castle","year":"2015","journal-title":"Am. J. Clin. Pathol."},{"issue":"3","key":"10.1016\/j.aca.2025.344830_bib11","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1039\/C5LC01388G","article-title":"An integrated paper-based sample-to-answer biosensor for nucleic acid testing at the point of care","volume":"16","author":"Choi","year":"2016","journal-title":"Lab Chip"},{"key":"10.1016\/j.aca.2025.344830_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2025.343994","article-title":"PCR-based SNP genotyping: a comprehensive comparison of methods for affordable and accurate detection of class IV mutations","volume":"1354","author":"Muneeswaran","year":"2025","journal-title":"Anal. Chim. Acta"},{"issue":"2","key":"10.1016\/j.aca.2025.344830_bib13","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.jcv.2013.01.014","article-title":"Comparison of the clinical performance of restriction fragment mass polymorphism (RFMP) and Roche linear array HPV test assays for HPV detection and genotyping","volume":"57","author":"Lee","year":"2013","journal-title":"J. Clin. Virol."},{"issue":"28","key":"10.1016\/j.aca.2025.344830_bib14","doi-asserted-by":"crossref","first-page":"7451","DOI":"10.1007\/s00216-019-02113-5","article-title":"A simple and efficient method for potential point-of-care diagnosis of human papillomavirus genotypes: combination of isothermal recombinase polymerase amplification with lateral flow dipstick and reverse dot blot","volume":"411","author":"Ma","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"issue":"1\u20132","key":"10.1016\/j.aca.2025.344830_bib15","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.jviromet.2012.10.014","article-title":"Rapid genotyping of carcinogenic human papillomavirus by loop-mediated isothermal amplification using a new automated DNA test (Clinichip HPV\u2122)","volume":"188","author":"Satoh","year":"2013","journal-title":"J. Virol. Methods"},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib16","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1186\/s12885-018-4087-1","article-title":"Assessing the performance of a Loop Mediated Isothermal Amplification (LAMP) assay for the detection and subtyping of high-risk suptypes of Human Papilloma Virus (HPV) for Oropharyngeal Squamous Cell Carcinoma (OPSCC) without DNA purification","volume":"18","author":"Rohatensky","year":"2018","journal-title":"BMC Cancer"},{"issue":"9","key":"10.1016\/j.aca.2025.344830_bib17","doi-asserted-by":"crossref","first-page":"2637","DOI":"10.7150\/thno.32224","article-title":"Synergistically enhanced colorimetric molecular detection using smart cup: a case for instrument-free HPV-associated cancer screening","volume":"9","author":"Yin","year":"2019","journal-title":"Theranostics"},{"issue":"24","key":"10.1016\/j.aca.2025.344830_bib18","doi-asserted-by":"crossref","first-page":"6199","DOI":"10.1007\/s00216-020-02702-9","article-title":"Method for the elucidation of LAMP products captured on lateral flow strips in a point of care test for HPV 16","volume":"412","author":"Landaverde","year":"2020","journal-title":"Anal. Bioanal. Chem."},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib19","first-page":"27","article-title":"Isothermal method of a recombinase polymerase amplification assay for the detection of Most common high-risk human papillomavirus type 16 and type 18 DNA","volume":"63","author":"Ma","year":"2017","journal-title":"Clin. Lab."},{"issue":"6336","key":"10.1016\/j.aca.2025.344830_bib20","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.aca.2025.344830_bib21","article-title":"Dynamic DNA assembly-assisted CRISPR\/Cas12a system for lung cancer-associated miRNA analysis","volume":"32","author":"Sheng","year":"2025","journal-title":"Mater. Today Bio"},{"key":"10.1016\/j.aca.2025.344830_bib22","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/s41421-018-0028-z","article-title":"CRISPR-Cas12a-assisted nucleic acid detection","volume":"4","author":"Li","year":"2018","journal-title":"Cell Discov"},{"issue":"6387","key":"10.1016\/j.aca.2025.344830_bib23","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.aca.2025.344830_bib24","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-92329-2","article-title":"A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology","volume":"11","author":"Gong","year":"2021","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib25","doi-asserted-by":"crossref","first-page":"6480","DOI":"10.1038\/s41467-022-34086-y","article-title":"Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification","volume":"13","author":"Xu","year":"2022","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib26","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1186\/s12985-024-02439-2","article-title":"The cervical cancer related distribution, coinfection and risk of 15 HPV types in Baoan, Shenzhen, in 2017-2023","volume":"21","author":"Li","year":"2024","journal-title":"Virol. J."},{"key":"10.1016\/j.aca.2025.344830_bib27","doi-asserted-by":"crossref","DOI":"10.3389\/fpubh.2023.1145396","article-title":"High-risk HPV prevalence and genotype distribution among women in Liaocheng, Shandong Province, China from 2016 to 2022","volume":"11","author":"Zheng","year":"2023","journal-title":"Front. Public Health"},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib28","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1186\/s12985-021-01676-z","article-title":"Prevalence, characteristics, and distribution of HPV genotypes in women from Zhejiang Province, 2016-2020","volume":"18","author":"Yan","year":"2021","journal-title":"Virol. J."},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib29","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s11658-024-00548-y","article-title":"A multiplex RPA-CRISPR\/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay","volume":"29","author":"Liu","year":"2024","journal-title":"Cell. Mol. Biol. Lett."},{"key":"10.1016\/j.aca.2025.344830_bib30","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1220522","article-title":"The correlation between multiple HPV infections and the occurrence, development, and prognosis of cervical cancer","volume":"14","author":"Na","year":"2023","journal-title":"Front. Microbiol."},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib31","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1007\/s11033-023-08939-8","article-title":"Comparison of high-risk HPV detection by the AmpFire\u00ae HPV screening 16\/18\/HR technique (Atila Biosystems) and the hybrid capture 2 test (Qiagen)","volume":"51","author":"Koussouri","year":"2024","journal-title":"Mol. Biol. Rep."},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib32","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1186\/s12885-024-12141-9","article-title":"Positivity rates, trends and experiences of health workers on human papillomavirus screened using genexpert in Uganda: a three-year retrospective cohort study","volume":"24","author":"Nakigozi","year":"2024","journal-title":"BMC Cancer"},{"issue":"12","key":"10.1016\/j.aca.2025.344830_bib33","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1038\/s41416-020-01111-0","article-title":"Deep sequencing detects human papillomavirus (HPV) in cervical cancers negative for HPV by PCR","volume":"123","author":"Arroyo M\u00fchr","year":"2020","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.aca.2025.344830_bib34","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.meegid.2018.11.008","article-title":"Phylogenetic analysis of Alphapapillomavirus based on L1, E6 and E7 regions suggests that carcinogenicity and tissue tropism have appeared multiple times during viral evolution","volume":"67","author":"Rojas-Cruz","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.aca.2025.344830_bib35","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.3389\/fmicb.2020.01015","article-title":"A recombinase polymerase amplification and lateral flow strip combined method that detects Salmonella enterica Serotype typhimurium with no worry of primer-dependent artifacts","volume":"11","author":"Wu","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.aca.2025.344830_bib36","doi-asserted-by":"crossref","first-page":"2959","DOI":"10.3389\/fmicb.2019.02959","article-title":"Rapid and specific detection of Listeria monocytogenes with an isothermal amplification and lateral flow strip combined method that eliminates false-positive signals from primer-dimers","volume":"10","author":"Wang","year":"2020","journal-title":"Front. Microbiol."},{"issue":"2","key":"10.1016\/j.aca.2025.344830_bib37","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1520\/JFS13607J","article-title":"Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification","volume":"39","author":"Akane","year":"1994","journal-title":"J. Forensic Sci."},{"issue":"1","key":"10.1016\/j.aca.2025.344830_bib38","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1039\/C8AN01621F","article-title":"Review: a comprehensive summary of a decade development of the recombinase polymerase amplification","volume":"144","author":"Li","year":"2018","journal-title":"Analyst"},{"key":"10.1016\/j.aca.2025.344830_bib39","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/1475-2875-13-99","article-title":"Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis","volume":"13","author":"Kersting","year":"2014","journal-title":"Malar. J."},{"issue":"14","key":"10.1016\/j.aca.2025.344830_bib40","doi-asserted-by":"crossref","first-page":"8257","DOI":"10.1021\/acs.jafc.4c00204","article-title":"Agarose Hydrogel-Boosted one-tube RPA-CRISPR\/Cas12a assay for robust point-of-care detection of Zoonotic nematode anisakis","volume":"72","author":"Zhao","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.aca.2025.344830_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2024.342693","article-title":"Sensitive and visual detection of SARS-CoV-2 using RPA-Cas12a one-step assay with ssDNA-modified crRNA","volume":"1309","author":"Zeng","year":"2024","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.344830_bib42","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2023.134771","article-title":"High-throughput centrifugal microfluidic platform for multiplex respiratory virus diagnostics","volume":"399","author":"Nguyen","year":"2024","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.aca.2025.344830_bib43","doi-asserted-by":"crossref","DOI":"10.2991\/978-94-6463-564-5","article-title":"Development of a novel Cas13a\/Cas12a-mediated \u2018one-pot\u2019 dual detection assay for genetically modified crops","author":"Ding","year":"2024","journal-title":"J. Adv. Res."},{"key":"10.1016\/j.aca.2025.344830_bib44","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2021.113701","article-title":"Exploiting the orthogonal CRISPR-Cas12a\/Cas13a trans-cleavage for dual-gene virus detection using a handheld device","volume":"196","author":"Tian","year":"2022","journal-title":"Biosens. Bioelectron."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025012243?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025012243?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:20:02Z","timestamp":1773274802000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267025012243"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":44,"alternative-id":["S0003267025012243"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2025.344830","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel multiplex RPA\/CRISPR-Cas12a integrated biosensor for on-site detection of high-risk HPV genotypes","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2025.344830","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"344830"}}