{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T02:22:29Z","timestamp":1773886949542,"version":"3.50.1"},"reference-count":76,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC3702700"],"award-info":[{"award-number":["2022YFC3702700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82025030"],"award-info":[{"award-number":["82025030"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82241091"],"award-info":[{"award-number":["82241091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.aca.2025.344994","type":"journal-article","created":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T07:36:29Z","timestamp":1765006589000},"page":"344994","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Development of a high-throughput LLE-UPLC-QqQ-MS\/MS method and comprehensive profiling of urinary steroid hormones in Chinese elderly adults"],"prefix":"10.1016","volume":"1385","author":[{"given":"Xiao","family":"Ma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huimin","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojie","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiran","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaona","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengyao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2563-8030","authenticated-orcid":false,"given":"Yifu","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shilu","family":"Tong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyang","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu'e","family":"Cha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoming","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"36","key":"10.1016\/j.aca.2025.344994_bib1","doi-asserted-by":"crossref","first-page":"7064","DOI":"10.1021\/bi300895w","article-title":"Minor activities and transition state properties of the human steroid hydroxylases cytochromes P450c17 and P450c21, from reactions observed with deuterium-labeled substrates","volume":"51","author":"Yoshimoto","year":"2012","journal-title":"Biochemistry"},{"issue":"6","key":"10.1016\/j.aca.2025.344994_bib2","first-page":"1363","article-title":"Advances in chromatography-mass spectrometry technology of urinary steroid hormones and their metabolites","volume":"37","author":"Ma","year":"2024","journal-title":"Res. Environ. Sci."},{"issue":"1","key":"10.1016\/j.aca.2025.344994_bib3","first-page":"3","article-title":"Androgen receptor structure, function and biology: from bench to bedside","volume":"37","author":"Davey","year":"2016","journal-title":"Clin. Biochem. Rev."},{"issue":"8","key":"10.1016\/j.aca.2025.344994_bib4","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/1043-2760(94)90165-1","article-title":"Nongenomic actions of steroid hormones","volume":"5","author":"Wehling","year":"1994","journal-title":"Trends Endocrinol. Metabol."},{"key":"10.1016\/j.aca.2025.344994_bib5","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1351\/pac200375111859","article-title":"Naturally produced steroid hormones and their release into the environment","volume":"75","author":"Shore","year":"2003","journal-title":"Pure Appl. Chem."},{"key":"10.1016\/j.aca.2025.344994_bib6","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.jmsacl.2022.12.004","article-title":"A novel fully-automated method to measure steroids in serum by liquid chromatography-tandem mass spectrometry","volume":"27","author":"Fraissinet","year":"2023","journal-title":"J. Mass. Spectrom. Adv. Clin. Lab."},{"issue":"1","key":"10.1016\/j.aca.2025.344994_bib7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aquatox.2010.07.005","article-title":"Comparative biological effects and potency of 17\u03b1- and 17\u03b2-estradiol in fathead minnows","volume":"100","author":"Shappell","year":"2010","journal-title":"Aquat. Toxicol."},{"issue":"10","key":"10.1016\/j.aca.2025.344994_bib8","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1515\/cclm-2023-0141","article-title":"Salivary cortisol and cortisone in diagnosis of Cushing's syndrome - a comparison of six different analytical methods","volume":"61","author":"B\u00e4cklund","year":"2023","journal-title":"Clin. Chem. Lab. Med."},{"issue":"11","key":"10.1016\/j.aca.2025.344994_bib9","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1093\/gerona\/gls068","article-title":"The role of androgens and estrogens on healthy aging and longevity","volume":"67","author":"Horstman","year":"2012","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"issue":"1","key":"10.1016\/j.aca.2025.344994_bib10","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.ccell.2009.10.012","article-title":"Urinalysis: a review of methods and procedures","volume":"22","author":"Wu","year":"2010","journal-title":"Crit Care Nurs. Clin. North Am."},{"issue":"3","key":"10.1016\/j.aca.2025.344994_bib11","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1515\/jomb-2017-0056","article-title":"Comparison of widely used biochemical analytes in the serum and saliva samples of dialysis patients","volume":"37","author":"Alpdemir","year":"2018","journal-title":"J. Med. Biochem."},{"key":"10.1016\/j.aca.2025.344994_bib12","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.clinbiochem.2019.08.009","article-title":"Association between FSH, E1, and E2 levels in urine and serum in premenopausal and postmenopausal women","volume":"73","author":"Onizuka","year":"2019","journal-title":"Clin. Biochem."},{"issue":"2","key":"10.1016\/j.aca.2025.344994_bib13","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1124\/pr.109.002071","article-title":"Estrogen actions in the brain and the basis for differential action in men and women: a case for sex-specific medicines","volume":"62","author":"Gillies","year":"2010","journal-title":"Pharmacol. Rev."},{"issue":"3","key":"10.1016\/j.aca.2025.344994_bib14","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.steroids.2010.11.005","article-title":"Serum steroid profiling by isotopic dilution-liquid chromatography-mass spectrometry: comparison with current immunoassays and reference intervals in healthy adults","volume":"76","author":"Fanelli","year":"2011","journal-title":"Steroids"},{"key":"10.1016\/j.aca.2025.344994_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2024.141598","article-title":"Assessment of occurrence, partitioning and ecological risk for 144 steroid hormones in Taihu Lake using UPLC-MS\/MS with machine learning model","volume":"354","author":"Zhao","year":"2024","journal-title":"Chemosphere"},{"key":"10.1016\/j.aca.2025.344994_bib16","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.clinbiochem.2020.03.008","article-title":"Advancements in the gold standard: measuring steroid sex hormones by mass spectrometry","volume":"82","author":"Conklin","year":"2020","journal-title":"Clin. Biochem."},{"issue":"1","key":"10.1016\/j.aca.2025.344994_bib17","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1002\/dta.2255","article-title":"Power of Orbitrap-based LC-high resolution-MS\/MS for comprehensive drug testing in urine with or without conjugate cleavage or using dried urine spots after on-spot cleavage in comparison to established LC-MS(n) or GC-MS procedures","volume":"10","author":"Michely","year":"2018","journal-title":"Drug Test. Anal."},{"issue":"10","key":"10.1016\/j.aca.2025.344994_bib18","doi-asserted-by":"crossref","first-page":"3971","DOI":"10.1210\/jc.2013-3375","article-title":"Requirement for mass spectrometry sex steroid assays in the journal of clinical endocrinology and metabolism","volume":"98","author":"Handelsman","year":"2013","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"10.1016\/j.aca.2025.344994_bib19","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2023.1164679","article-title":"Characteristics of steroid hormones in systemic lupus erythematosus revealed by GC\/MS-based metabolic profiling","volume":"14","author":"Wu","year":"2023","journal-title":"Front. Endocrinol."},{"key":"10.1016\/j.aca.2025.344994_bib20","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.aca.2015.11.041","article-title":"Potential of atmospheric pressure chemical ionization source in gas chromatography tandem mass spectrometry for the screening of urinary exogenous androgenic anabolic steroids","volume":"906","author":"Raro","year":"2016","journal-title":"Anal. Chim. Acta"},{"issue":"Suppl 1","key":"10.1016\/j.aca.2025.344994_bib21","article-title":"Determination of hormones in human urine by ultra-high-performance liquid chromatography\/triple-quadrupole mass spectrometry","volume":"34","author":"Zhou","year":"2020","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"10.1016\/j.aca.2025.344994_bib22","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.talanta.2014.03.010","article-title":"Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: a novel approach for clinical monitoring and doping control","volume":"125","author":"Guo","year":"2014","journal-title":"Talanta"},{"key":"10.1016\/j.aca.2025.344994_bib23","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.jpba.2014.12.028","article-title":"Automated hollow fiber microextraction based on two immiscible organic solvents for the extraction of two hormonal drugs","volume":"107","author":"Tajik","year":"2015","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.aca.2025.344994_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.jchromb.2020.122390","article-title":"Quantification of steroid hormones in human urine by DLLME and UHPLC-HRMS detection","volume":"1159","author":"Dmitrieva","year":"2020","journal-title":"J. Chromatogr., B: Anal. Technol. Biomed. Life Sci."},{"issue":"17","key":"10.1016\/j.aca.2025.344994_bib25","doi-asserted-by":"crossref","DOI":"10.1002\/jssc.202300280","article-title":"Polyaniline-silica doped with oxalic acid as a novel extractor phase in thin film solid-phase microextraction for determination of hormones in urine","volume":"46","author":"Turazzi","year":"2023","journal-title":"J. Separ. Sci."},{"issue":"8","key":"10.1016\/j.aca.2025.344994_bib26","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1007\/s10337-018-3644-8","article-title":"Recent developments in selective materials for solid phase extraction","volume":"82","author":"Madikizela","year":"2019","journal-title":"Chromatographia"},{"issue":"1","key":"10.1016\/j.aca.2025.344994_bib27","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1002\/jssc.201800804","article-title":"Applications of solid-phase micro-extraction with mass spectrometry in pesticide analysis","volume":"42","author":"Liang","year":"2019","journal-title":"J. Separ. Sci."},{"issue":"1","key":"10.1016\/j.aca.2025.344994_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.trac.2009.10.003","article-title":"Liquid-phase microextraction","volume":"29","author":"Sarafraz-Yazdi","year":"2010","journal-title":"TrAC, Trends Anal. Chem."},{"key":"10.1016\/j.aca.2025.344994_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodres.2020.109712","article-title":"Advancement in analytical techniques for the extraction of grape and wine volatile compounds","volume":"137","author":"Mar\u00edn-San Rom\u00e1n","year":"2020","journal-title":"Food Res. Int."},{"key":"10.1016\/j.aca.2025.344994_bib30","series-title":"Comprehensive Sampling and Sample Preparation","first-page":"27","article-title":"Sampling techniques for the determination of volatile components in grape juice, wine and alcoholic beverages","author":"Costa Freitas","year":"2012"},{"issue":"122","key":"10.1016\/j.aca.2025.344994_bib31","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1039\/an9699400728","article-title":"An automated solvent-extraction technique for radiochemical group separations: application to the analysis of glass fragments by thermal-neutron activation","volume":"94","author":"Goode","year":"1969","journal-title":"Analyst"},{"key":"10.1016\/j.aca.2025.344994_bib32","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.chroma.2018.08.030","article-title":"Preparation and application of guanidyl-functionalized graphene oxide-grafted silica for efficient extraction of acidic herbicides by Box-Behnken design","volume":"1571","author":"Hou","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.aca.2025.344994_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2021.106866","article-title":"The exposome in practice: an exploratory panel study of biomarkers of air pollutant exposure in Chinese people aged 60-69 years (China BAPE study)","volume":"157","author":"Tang","year":"2021","journal-title":"Environ. Int."},{"issue":"12","key":"10.1016\/j.aca.2025.344994_bib34","doi-asserted-by":"crossref","first-page":"2620","DOI":"10.1002\/jssc.201700123","article-title":"Dispersive liquid-liquid microextraction as an effective preanalytical step for the determination of estradiol in human urine","volume":"40","author":"Kupcov\u00e1","year":"2017","journal-title":"J. Separ. Sci."},{"key":"10.1016\/j.aca.2025.344994_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2022.114736","article-title":"Solid-phase analytical derivatization as a tool for the quantification of steroid hormones in human urine with HPLC-Q-ToF detection","volume":"214","author":"Dmitrieva","year":"2022","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.aca.2025.344994_bib36","series-title":"Bioanalytical Method Validation Guidance for Industry","year":"2018"},{"key":"10.1016\/j.aca.2025.344994_bib37","series-title":"Definition and Procedure for the Determination of the Method Detection Limit","year":"2016"},{"issue":"5","key":"10.1016\/j.aca.2025.344994_bib38","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1208\/s12248-024-00974-y","article-title":"ICH M10 bioanalytical method validation Guideline-1 year later","volume":"26","author":"Vazvaei-Smith","year":"2024","journal-title":"AAPS J."},{"issue":"1","key":"10.1016\/j.aca.2025.344994_bib39","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3343\/alm.2023.43.1.5","article-title":"Calibration practices in clinical mass spectrometry: review and recommendations","volume":"43","author":"Cheng","year":"2023","journal-title":"Ann. Lab. Med."},{"key":"10.1016\/j.aca.2025.344994_bib40","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jsbmb.2015.10.003","article-title":"Chemical derivatization for enhancing sensitivity during LC\/ESI-MS\/MS quantification of steroids in biological samples: a review","volume":"162","author":"Higashi","year":"2016","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.aca.2025.344994_bib41","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.jpba.2011.10.021","article-title":"Comprehensive investigation of the influence of acidic, basic, and organic mobile phase compositions on bioanalytical assay sensitivity in positive ESI mode LC\/MS\/MS","volume":"59","author":"Rainville","year":"2012","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"11","key":"10.1016\/j.aca.2025.344994_bib42","doi-asserted-by":"crossref","first-page":"7772","DOI":"10.1021\/acsomega.7b01445","article-title":"Systematic optimization of liquid-liquid extraction for isolation of unidentified components","volume":"2","author":"Tshepelevitsh","year":"2017","journal-title":"ACS Omega"},{"key":"10.1016\/j.aca.2025.344994_bib43","series-title":"Handbook of Biomolecules","first-page":"227","article-title":"Chapter 10 - liquid-liquid extraction","author":"Jisha","year":"2023"},{"issue":"11","key":"10.1016\/j.aca.2025.344994_bib44","doi-asserted-by":"crossref","DOI":"10.3390\/toxics10110687","article-title":"Determination of 19 steroid hormones in human serum and urine using liquid chromatography-tandem mass spectrometry","volume":"10","author":"Li","year":"2022","journal-title":"Toxics"},{"issue":"1","key":"10.1016\/j.aca.2025.344994_bib45","doi-asserted-by":"crossref","first-page":"2678","DOI":"10.1038\/s41598-017-02732-x","article-title":"Liquid-liquid extraction intensification by micro-droplet rotation in a hydrocyclone","volume":"7","author":"Huang","year":"2017","journal-title":"Sci. Rep."},{"issue":"2","key":"10.1016\/j.aca.2025.344994_bib46","doi-asserted-by":"crossref","DOI":"10.3390\/molecules24020334","article-title":"Perspectives on the use of liquid extraction for radioisotope purification","volume":"24","author":"Martini","year":"2019","journal-title":"Molecules"},{"key":"10.1016\/j.aca.2025.344994_bib47","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.seppur.2019.01.041","article-title":"Separation of hemicelluloses and lignins from synthetic hydrolyzate and thermomechanical pulp mill process water via liquid-liquid extraction","volume":"215","author":"Bokhary","year":"2019","journal-title":"Separ. Purif. Technol."},{"issue":"6","key":"10.1016\/j.aca.2025.344994_bib48","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.jiec.2010.05.003","article-title":"Effect of aqueous phase pH on liquid\u2013liquid extraction with impinging-jets contacting technique","volume":"16","author":"Saien","year":"2010","journal-title":"J. Ind. Eng. Chem."},{"key":"10.1016\/j.aca.2025.344994_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2021.462235","article-title":"Development of a pH-dependent homogeneous liquid-liquid extraction by cold-induced phase separation in acetonitrile\/water mixtures for determination of quinolone residues in animal-derived foods","volume":"1649","author":"Hu","year":"2021","journal-title":"J. Chromatogr. A"},{"issue":"6","key":"10.1016\/j.aca.2025.344994_bib50","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/j.beem.2013.10.008","article-title":"Determination of cortisol in serum, saliva and urine","volume":"27","author":"Turpeinen","year":"2013","journal-title":"Best Pract. Res. Clin. Endocrinol. Metabol."},{"issue":"4","key":"10.1016\/j.aca.2025.344994_bib51","doi-asserted-by":"crossref","first-page":"285","DOI":"10.3343\/alm.2020.40.4.285","article-title":"Cortisol measurements in cushing's syndrome: immunoassay or mass spectrometry?","volume":"40","author":"Casals","year":"2020","journal-title":"Ann. Lab. Med."},{"key":"10.1016\/j.aca.2025.344994_bib52","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2019.113531","article-title":"Development and validation of LC-ESI-MS\/MS methods for quantification of 27 free and conjugated estrogen-related metabolites","volume":"590","author":"van der Berg","year":"2020","journal-title":"Anal. Biochem."},{"issue":"2","key":"10.1016\/j.aca.2025.344994_bib53","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1042\/bj1770729","article-title":"The direct radioimmunoassay of oestrogen glucuronides in human female urine","volume":"177","author":"Baker","year":"1979","journal-title":"Biochem. J."},{"issue":"11","key":"10.1016\/j.aca.2025.344994_bib54","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1055\/s-0028-1082042","article-title":"Enzyme immunoassay of testosterone, 17beta-estradiol, and progesterone in perspiration and urine of preadolescents and young adults: exceptional levels in men's axillary perspiration","volume":"40","author":"Muir","year":"2008","journal-title":"Horm. Metab. Res."},{"issue":"1\u20132","key":"10.1016\/j.aca.2025.344994_bib55","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.jchromb.2007.11.009","article-title":"Development of a liquid chromatography-tandem mass spectrometry method for the determination of 23 endogenous steroids in small quantities of primate urine","volume":"862","author":"Hauser","year":"2008","journal-title":"J. Chromatogr., B: Anal. Technol. Biomed. Life Sci."},{"issue":"3","key":"10.1016\/j.aca.2025.344994_bib56","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1038\/s43587-024-00601-7","article-title":"Compromised biogenesis of DHEA-S is implicated in primate adrenal aging","volume":"4","year":"2024","journal-title":"Nat. Aging"},{"issue":"1","key":"10.1016\/j.aca.2025.344994_bib57","doi-asserted-by":"crossref","DOI":"10.3390\/ph15010046","article-title":"The utilization of dehydroepiandrosterone as a sexual hormone precursor in premenopausal and postmenopausal women: an overview","volume":"15","author":"Tang","year":"2021","journal-title":"Pharmaceuticals"},{"issue":"6","key":"10.1016\/j.aca.2025.344994_bib58","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1007\/s43440-024-00654-w","article-title":"Targeting glucocorticoid receptor signaling pathway for treatment of stress-related brain disorders","volume":"76","author":"G\u00f6ver","year":"2024","journal-title":"Pharmacol. Rep."},{"issue":"23","key":"10.1016\/j.aca.2025.344994_bib59","doi-asserted-by":"crossref","DOI":"10.3390\/cells12232726","article-title":"The role of cortisol in chronic stress, neurodegenerative diseases, and psychological disorders","volume":"12","author":"Knezevic","year":"2023","journal-title":"Cells"},{"key":"10.1016\/j.aca.2025.344994_bib60","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.chroma.2018.10.041","article-title":"Hexafluoroisopropanol-alkyl carboxylic acid high-density supramolecular solvent based dispersive liquid-liquid microextraction of steroid sex hormones in human urine","volume":"1580","author":"Zong","year":"2018","journal-title":"J. Chromatogr. A"},{"issue":"10","key":"10.1016\/j.aca.2025.344994_bib61","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1152\/physiolgenomics.00006.2016","article-title":"The role of the hypothalamic-pituitary-adrenal axis in modulating seasonal changes in immunity","volume":"48","author":"Pierre","year":"2016","journal-title":"Physiol. Genom."},{"key":"10.1016\/j.aca.2025.344994_bib62","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.63425","article-title":"Sex differences in biological aging with a focus on human studies","volume":"10","author":"H\u00e4gg","year":"2021","journal-title":"eLife"},{"issue":"2","key":"10.1016\/j.aca.2025.344994_bib63","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/cen.14342","article-title":"Sociodemographic, lifestyle and medical influences on serum testosterone and sex hormone-binding globulin in men from UK biobank","volume":"94","author":"Yeap","year":"2021","journal-title":"Clin. Endocrinol."},{"issue":"2","key":"10.1016\/j.aca.2025.344994_bib64","doi-asserted-by":"crossref","DOI":"10.1002\/bmc.4723","article-title":"Development and validation of an LC-MS\/MS method for profiling 39 urinary steroids (estrogens, androgens, corticoids, and progestins)","volume":"34","author":"Son","year":"2020","journal-title":"Biomed. Chromatogr."},{"issue":"5","key":"10.1016\/j.aca.2025.344994_bib65","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.beem.2013.05.015","article-title":"Novel liquid chromatography tandem mass spectrometry (LC-MS\/MS) methods for measuring steroids","volume":"27","author":"Keevil","year":"2013","journal-title":"Best Pract. Res. Clin. Endocrinol. Metabol."},{"issue":"1\u20132","key":"10.1016\/j.aca.2025.344994_bib66","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.mce.2012.12.026","article-title":"Mass spectrometry theory and application to adrenal diseases","volume":"371","author":"Wooding","year":"2013","journal-title":"Mol. Cell. Endocrinol."},{"issue":"1\u20132","key":"10.1016\/j.aca.2025.344994_bib67","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.chroma.2008.01.045","article-title":"Direct quantification of steroid glucuronides in human urine by liquid chromatography-electrospray tandem mass spectrometry","volume":"1183","author":"Pozo","year":"2008","journal-title":"J. Chromatogr. A"},{"issue":"19","key":"10.1016\/j.aca.2025.344994_bib68","doi-asserted-by":"crossref","first-page":"4689","DOI":"10.1007\/s00216-017-0823-7","article-title":"Magnetic ionic liquids as versatile extraction phases for the rapid determination of estrogens in human urine by dispersive liquid-liquid microextraction coupled with high-performance liquid chromatography-diode array detection","volume":"410","author":"Merib","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.aca.2025.344994_bib69","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.talanta.2017.09.068","article-title":"Novel generation of deep eutectic solvent as an acceptor phase in three-phase hollow fiber liquid phase microextraction for extraction and preconcentration of steroidal hormones from biological fluids","volume":"178","author":"Khataei","year":"2018","journal-title":"Talanta"},{"issue":"12","key":"10.1016\/j.aca.2025.344994_bib70","doi-asserted-by":"crossref","first-page":"4313","DOI":"10.1007\/s00216-013-6779-3","volume":"405","author":"Wang","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"issue":"3","key":"10.1016\/j.aca.2025.344994_bib71","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1002\/jssc.201501156","article-title":"Multiple monolithic fiber solid-phase microextraction based on a polymeric ionic liquid with high-performance liquid chromatography for the determination of steroid sex hormones in water and urine","volume":"39","author":"Liao","year":"2016","journal-title":"J. Separ. Sci."},{"issue":"48","key":"10.1016\/j.aca.2025.344994_bib72","doi-asserted-by":"crossref","first-page":"7461","DOI":"10.1016\/j.chroma.2010.08.056","article-title":"Preparation and application of solid-phase microextraction fiber based on molecularly imprinted polymer for determination of anabolic steroids in complicated samples","volume":"1217","author":"Qiu","year":"2010","journal-title":"J. Chromatogr. A"},{"issue":"5","key":"10.1016\/j.aca.2025.344994_bib73","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.jpba.2010.02.027","article-title":"Determination of anabolic steroids in human urine by automated in-tube solid-phase microextraction coupled with liquid chromatography-mass spectrometry","volume":"52","author":"Saito","year":"2010","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"1","key":"10.1016\/j.aca.2025.344994_bib74","first-page":"172","article-title":"Determination of steroid sex hormones in urine matrix by stir bar sorptive extraction based on monolithic material and liquid chromatography with diode array detection","volume":"75","author":"Huang","year":"2008","journal-title":"Talanta"},{"key":"10.1016\/j.aca.2025.344994_bib75","doi-asserted-by":"crossref","DOI":"10.1016\/j.jchromb.2022.123406","article-title":"Determination of hormones in urine by hollow fiber microporous membrane liquid-liquid extraction associated with 96-well plate system and HPLC-FLD detection","volume":"1207","author":"Lopes","year":"2022","journal-title":"J. Chromatogr., B: Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.aca.2025.344994_bib76","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2024.464735","article-title":"Determination of urinary androgens in women with polycystic ovary syndrome using LC-QqQ\/MS and the application of thin film solid-phase microextraction (TF-SPME)","volume":"1718","author":"Rajska","year":"2024","journal-title":"J. Chromatogr. A"}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025013881?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025013881?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T21:38:44Z","timestamp":1773869924000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267025013881"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":76,"alternative-id":["S0003267025013881"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2025.344994","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a high-throughput LLE-UPLC-QqQ-MS\/MS method and comprehensive profiling of urinary steroid hormones in Chinese elderly adults","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2025.344994","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"344994"}}