{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T03:33:21Z","timestamp":1793072001808,"version":"4.5.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:00:00Z","timestamp":1765324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/50011\/2025"],"award-info":[{"award-number":["UID\/50011\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.aca.2025.345001","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T07:56:33Z","timestamp":1765353393000},"page":"345001","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Ionic-liquid-based aqueous biphasic systems meet microfluidics: from antibodies to aptamers in prostate-specific antigen detection"],"prefix":"10.1016","volume":"1385","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6631-9821","authenticated-orcid":false,"given":"Maria S.M.","family":"Mendes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5306-4409","authenticated-orcid":false,"given":"Virginia","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8895-0614","authenticated-orcid":false,"given":"Mara G.","family":"Freire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6735-5437","authenticated-orcid":false,"given":"Francisca A.","family":"e Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5677-3024","authenticated-orcid":false,"given":"Jo\u00e3o P.","family":"Conde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2025.345001_bib1","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. Clin."},{"key":"10.1016\/j.aca.2025.345001_bib2","doi-asserted-by":"crossref","DOI":"10.1177\/17562872221103988","article-title":"Biomarkers for prostate cancer detection and risk stratification","volume":"14","author":"Farha","year":"2022","journal-title":"Ther Adv Urol"},{"key":"10.1016\/j.aca.2025.345001_bib3","doi-asserted-by":"crossref","first-page":"2496","DOI":"10.1039\/c0an00204f","article-title":"Measurement of biomarker proteins for point-of-care early detection and monitoring of cancer","volume":"135","author":"Rusling","year":"2010","journal-title":"Analyst"},{"key":"10.1016\/j.aca.2025.345001_bib4","doi-asserted-by":"crossref","first-page":"3643","DOI":"10.1021\/acssensors.3c01402","article-title":"Point-of-Care prostate specific antigen testing: examining translational progress toward clinical implementation","volume":"8","author":"Garg","year":"2023","journal-title":"ACS Sens."},{"key":"10.1016\/j.aca.2025.345001_bib5","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1002\/adhm.201600009","article-title":"Recent progress of microfluidics in translational applications","volume":"5","author":"Liu","year":"2016","journal-title":"Adv. Healthcare Mater."},{"key":"10.1016\/j.aca.2025.345001_bib6","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.aca.2007.05.065","article-title":"Fully automated immunoassay for detection of prostate-specific antigen using nano-magnetic beads and micro-polystyrene bead composites, \u2018beads on beads","volume":"597","author":"Matsunaga","year":"2007","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.345001_bib7","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.urolonc.2021.03.010","article-title":"Prostate cancer and microfluids","volume":"39","author":"Bernstein","year":"2021","journal-title":"Urol. Oncol.: Seminars and Original Investigations"},{"key":"10.1016\/j.aca.2025.345001_bib8","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1016\/j.bios.2007.11.003","article-title":"Integrated microfluidic systems with an immunosensor modified with carbon nanotubes for detection of prostate specific antigen (PSA) in human serum samples","volume":"23","author":"Panini","year":"2008","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2025.345001_bib9","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1016\/j.bios.2010.11.039","article-title":"Microfluidic chip-based nanoelectrode array as miniaturized biochemical sensing platform for prostate-specific antigen detection","volume":"26","author":"Triroj","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2025.345001_bib10","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s11671-019-2857-6","article-title":"Microfluidic device directly fabricated on screen-printed electrodes for ultrasensitive electrochemical sensing of PSA","volume":"14","author":"Chen","year":"2019","journal-title":"Nanoscale Res. Lett."},{"key":"10.1016\/j.aca.2025.345001_bib11","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.biotechadv.2018.11.001","article-title":"Three decades of nucleic acid aptamer technologies: lessons learned, progress and opportunities on aptamer development","volume":"37","author":"Wang","year":"2019","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.aca.2025.345001_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2024.343004","article-title":"Process, advances, and perspectives of graphene oxide-SELEX for the development of aptamer molecular probes: a comprehensive review","volume":"1320","author":"Chen","year":"2024","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2025.345001_bib13","doi-asserted-by":"crossref","first-page":"13839","DOI":"10.3390\/s150613839","article-title":"A microfluidic love-wave biosensing device for PSA detection based on an aptamer beacon probe","volume":"15","author":"Zhang","year":"2015","journal-title":"Sensors"},{"key":"10.1016\/j.aca.2025.345001_bib14","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.bios.2017.10.027","article-title":"Reduced graphene oxide\/BiFeO3 nanohybrids-based signal-on photoelectrochemical sensing system for prostate-specific antigen detection coupling with magnetic microfluidic device","volume":"101","author":"Zhou","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2025.345001_bib15","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1002\/pmic.200400900","article-title":"Comparison of different depletion strategies for improved resolution in proteomic analysis of human serum samples","volume":"5","author":"Bj\u00f6rhall","year":"2005","journal-title":"Proteomics"},{"key":"10.1016\/j.aca.2025.345001_bib16","series-title":"Proteomic and Metabolomic Approaches to Biomarker Discovery","first-page":"95","article-title":"Sample depletion, fractionation, and enrichment for biomarker discovery","author":"Issaq","year":"2020"},{"key":"10.1016\/j.aca.2025.345001_bib17","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1002\/elps.200900286","article-title":"High\u2010abundance protein depletion: comparison of methods for human plasma biomarker discovery","volume":"31","author":"Polaskova","year":"2010","journal-title":"Electrophoresis"},{"key":"10.1016\/j.aca.2025.345001_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.seppur.2023.123875","article-title":"Aqueous two-phase systems as multipurpose tools to improve biomarker analysis","volume":"317","author":"Mendes","year":"2023","journal-title":"Sep. Purif. Technol."},{"key":"10.1016\/j.aca.2025.345001_bib19","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1016\/j.procbio.2010.03.026","article-title":"Potential of aqueous two-phase systems constructed on flexible devices: human serum albumin as proof of concept","volume":"45","author":"Garza-Madrid","year":"2010","journal-title":"Process Biochem."},{"key":"10.1016\/j.aca.2025.345001_bib20","doi-asserted-by":"crossref","first-page":"3184","DOI":"10.1002\/jssc.201200646","article-title":"Purification of transthyretin as nutritional biomarker of selenium status","volume":"35","author":"Mahn","year":"2012","journal-title":"J. Separ. Sci."},{"key":"10.1016\/j.aca.2025.345001_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2025.466249","article-title":"Extraction of aloe polysaccharides by recoverable aqueous biphasic system composed of thermo-sensitive magnetic ionic liquid and pH-sensitive copolymer","volume":"1759","author":"Yao","year":"2025","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.aca.2025.345001_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.seppur.2025.133786","article-title":"Isolation and purification of aloe anthraquinones through recoverable triple-responsive aqueous two-phase system","volume":"374","author":"Yao","year":"2025","journal-title":"Sep. Purif. Technol."},{"key":"10.1016\/j.aca.2025.345001_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.seppur.2023.124248","article-title":"Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systems","volume":"322","author":"Rosa","year":"2023","journal-title":"Sep. Purif. Technol."},{"key":"10.1016\/j.aca.2025.345001_bib24","doi-asserted-by":"crossref","first-page":"334","DOI":"10.3390\/bios13030334","article-title":"Combined use of ionic liquid-based aqueous biphasic systems and microfluidic devices for the detection of prostate-specific antigen","volume":"13","author":"Flora","year":"2023","journal-title":"Biosensors (Basel)"},{"key":"10.1016\/j.aca.2025.345001_bib25","doi-asserted-by":"crossref","first-page":"4284","DOI":"10.1039\/C4LC00695J","article-title":"On-chip sample preparation and analyte quantification using a microfluidic aqueous two-phase extraction coupled with an immunoassay","volume":"14","author":"Soares","year":"2014","journal-title":"Lab Chip"},{"key":"10.1016\/j.aca.2025.345001_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.seppur.2019.116019","article-title":"Performance of tetraalkylammonium-based ionic liquids as constituents of aqueous biphasic systems in the extraction of ovalbumin and lysozyme","volume":"233","author":"Belchior","year":"2020","journal-title":"Sep. Purif. Technol."},{"key":"10.1016\/j.aca.2025.345001_bib27","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0378-4347(97)00594-X","article-title":"Aqueous two-phase systems for protein separation studies on phase inversion","volume":"711","author":"Merchuk","year":"1998","journal-title":"J. Chromatogr., B: Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.aca.2025.345001_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114505","article-title":"Aptamer-based chemiluminescent optical fiber immunosensor with enhanced signal amplification for ultrasensitive detection of tumor biomarkers","volume":"214","author":"Zhao","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2025.345001_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2022.116530","article-title":"The ten principles of green sample preparation","volume":"148","author":"L\u00f3pez-Lorente","year":"2022","journal-title":"TrAC, Trends Anal. Chem."},{"key":"10.1016\/j.aca.2025.345001_bib30","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.bios.2015.12.058","article-title":"DNA aptamer-based sandwich microfluidic assays for dual quantification and multi-glycan profiling of cancer biomarkers","volume":"79","author":"Jolly","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2025.345001_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.virol.2023.109916","article-title":"Selection, activity, and nuclease stabilization of cross-neutralizing DNA aptamers targeting HSV-1 and HSV-2","volume":"589","author":"Schump","year":"2024","journal-title":"Virology"},{"key":"10.1016\/j.aca.2025.345001_bib32","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.colsurfb.2018.12.013","article-title":"Hybridization induced fluorescence enhanced DNA-Ag nanocluster\/aptamer probe for detection of prostate-specific antigen","volume":"175","author":"Fang","year":"2019","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.aca.2025.345001_bib33","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1016\/j.bios.2010.02.009","article-title":"Prostate specific antigen detection in patient sera by fluorescence-free BioCD protein array","volume":"26","author":"Wang","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2025.345001_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/250131","article-title":"Solid-phase extraction strategies to surmount body fluid sample complexity in high-throughput mass spectrometry-based proteomics","volume":"2015","author":"Bladergroen","year":"2015","journal-title":"J Anal Methods Chem"},{"key":"10.1016\/j.aca.2025.345001_bib35","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.crbiot.2021.11.003","article-title":"Highly portable quantitative screening test for prostate-specific antigen at point of care","volume":"3","author":"Srinivasan","year":"2021","journal-title":"Curr Res Biotechnol"}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025013959?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267025013959?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T03:56:30Z","timestamp":1773892590000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267025013959"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":35,"alternative-id":["S0003267025013959"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2025.345001","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ionic-liquid-based aqueous biphasic systems meet microfluidics: from antibodies to aptamers in prostate-specific antigen detection","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2025.345001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"345001"}}