{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T10:42:48Z","timestamp":1781606568347,"version":"3.54.5"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22374051"],"award-info":[{"award-number":["22374051"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.aca.2026.345133","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T23:57:13Z","timestamp":1768953433000},"page":"345133","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Double derivatization-enhanced LC-MS\/MS for isomer-resolved identification and quantification of fatty acids"],"prefix":"10.1016","volume":"1390","author":[{"given":"Xingyu","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuilin","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2276-7942","authenticated-orcid":false,"given":"Ting","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2026.345133_bib1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1146\/annurev-physiol-032122-030352","article-title":"Fatty acid transport and signaling: mechanisms and physiological implications","volume":"85","author":"Samovski","year":"2023","journal-title":"Annu. Rev. Physiol."},{"key":"10.1016\/j.aca.2026.345133_bib2","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1038\/s42255-023-00786-y","article-title":"Physiological and pathological roles of lipogenesis","volume":"5","author":"Jeon","year":"2023","journal-title":"Nat. Metab."},{"key":"10.1016\/j.aca.2026.345133_bib3","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1194\/jlr.R120000800","article-title":"Roles of polyunsaturated fatty acids, from mediators to membranes","volume":"61","author":"Harayama","year":"2020","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.aca.2026.345133_bib4","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1039\/D3FO03716A","article-title":"Fatty acid isomerism: analysis and selected biological functions","volume":"15","author":"Wang","year":"2024","journal-title":"Food Funct."},{"key":"10.1016\/j.aca.2026.345133_bib5","doi-asserted-by":"crossref","first-page":"5961","DOI":"10.1039\/D5FO01237F","article-title":"Molecular mechanisms of aberrant fatty acids metabolism in driving cardiovascular diseases: key regulatory targets and dietary interventions","volume":"16","author":"Yang","year":"2025","journal-title":"Food Funct."},{"key":"10.1016\/j.aca.2026.345133_bib6","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1093\/advances\/nmz125","article-title":"Mechanisms of action of trans fatty acids","volume":"11","author":"Oteng","year":"2020","journal-title":"Adv. Nutr."},{"key":"10.1016\/j.aca.2026.345133_bib7","doi-asserted-by":"crossref","first-page":"8386","DOI":"10.1021\/acs.analchem.0c00970","article-title":"Double bond characterization of free fatty acids directly from biological tissues by ultraviolet photodissociation","volume":"92","author":"Feider","year":"2020","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib8","doi-asserted-by":"crossref","first-page":"3940","DOI":"10.1038\/s41467-023-39617-9","article-title":"Ozone-enabled fatty acid discovery reveals unexpected diversity in the human lipidome","volume":"14","author":"Menzel","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2026.345133_bib9","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1002\/mas.21729","article-title":"Chemical derivatization strategy for mass spectrometry-based lipidomics","volume":"42","author":"Xia","year":"2023","journal-title":"Mass Spectrom. Rev."},{"key":"10.1016\/j.aca.2026.345133_bib10","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.aca.2020.04.064","article-title":"Quantitative profiling of eicosanoids derived from n-6 and n-3 polyunsaturated fatty acids by twin derivatization strategy combined with LC-MS\/MS in patients with type 2 diabetes mellitus","volume":"1120","author":"Xia","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2026.345133_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2025.344814","article-title":"Ozonization for MALDI-2 MS imaging of carbon\u2013carbon double bond positional isomers of phosphatidylethanolamines in biological tissues","volume":"1382","author":"Bezdekov\u00e1","year":"2026","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2026.345133_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2025.344456","article-title":"Review: deep lipidomic profiling enabled by the Patern\u00f2-B\u00fcchi reaction \u2013 pinpointing C=C locations and beyond","volume":"1376","author":"Zhao","year":"2025","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2026.345133_bib13","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1021\/acs.analchem.8b04905","article-title":"Identification of double bond position isomers in unsaturated lipids by m-CPBA epoxidation and mass spectrometry fragmentation","volume":"91","author":"Feng","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib14","doi-asserted-by":"crossref","first-page":"8115","DOI":"10.1039\/D1SC01614H","article-title":"Quantification and molecular imaging of fatty acid isomers from complex biological samples by mass spectrometry","volume":"12","author":"Zhang","year":"2021","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2026.345133_bib15","doi-asserted-by":"crossref","first-page":"10270","DOI":"10.1021\/acs.analchem.7b01870","article-title":"Identification and quantitation of C=C location isomers of unsaturated fatty acids by epoxidation reaction and tandem mass spectrometry","volume":"89","author":"Zhao","year":"2017","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib16","article-title":"Lipid mass tags via aziridination for probing unsaturated lipid isomers and accurate relative quantification","volume":"61","author":"Yang","year":"2022","journal-title":"Angew Chem. Int. Ed. Engl."},{"key":"10.1016\/j.aca.2026.345133_bib17","doi-asserted-by":"crossref","first-page":"4275","DOI":"10.1021\/acs.analchem.4c06680","article-title":"Structural lipidomics enabled by isomer-resolved tandem mass spectrometry","volume":"97","author":"Liu","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib18","doi-asserted-by":"crossref","first-page":"3083","DOI":"10.1021\/acs.analchem.4c06262","article-title":"Stereoselective reaction enabling simultaneous analysis of carbon-carbon double-bond configuration and the position of monounsaturated fatty acids through UHPLC-ESI-MRM-MS","volume":"97","author":"Yue","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib19","doi-asserted-by":"crossref","first-page":"29503","DOI":"10.1021\/jacs.4c08980","article-title":"Simultaneous determination of the position and cis-trans configuration of lipid C=C bonds via asymmetric derivatization and ion mobility-mass spectrometry","volume":"146","author":"Tang","year":"2024","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.aca.2026.345133_bib20","doi-asserted-by":"crossref","first-page":"17526","DOI":"10.1021\/acs.analchem.4c02697","article-title":"Mapping lipid C=C isomer profiles of human gut bacteria through a novel structural lipidomics workflow assisted by chemical epoxidation","volume":"96","author":"Chen","year":"2024","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib21","doi-asserted-by":"crossref","first-page":"7173","DOI":"10.1021\/acs.analchem.9b00374","article-title":"Analysis of conjugated fatty acid isomers by the Patern\u00f2-B\u00fcchi reaction and trapped ion mobility mass spectrometry","volume":"91","author":"Xie","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib22","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1021\/acs.analchem.8b04951","article-title":"Chemical tagging in mass spectrometry for systems biology","volume":"91","author":"Huang","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2022.116818","article-title":"Carboxyl-containing compounds in food: category, functions, and analysis with chemical derivatization-based LC-MS","volume":"157","author":"Zhang","year":"2022","journal-title":"Trends Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib24","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.aca.2014.11.015","article-title":"An isotope-labeled chemical derivatization method for the quantitation of short-chain fatty acids in human feces by liquid chromatography-tandem mass spectrometry","volume":"854","author":"Han","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2026.345133_bib25","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.foodchem.2017.09.069","article-title":"Rapid and sensitive detection of free fatty acids in edible oils based on chemical derivatization coupled with electrospray ionization tandem mass spectrometry","volume":"242","author":"Liu","year":"2018","journal-title":"Food Chem."},{"key":"10.1016\/j.aca.2026.345133_bib26","doi-asserted-by":"crossref","first-page":"6446","DOI":"10.1021\/acs.analchem.9b05588","article-title":"Double derivatization strategy for high-sensitivity and high-coverage localization of double bonds in free fatty acids by mass spectrometry","volume":"92","author":"Xu","year":"2020","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib27","doi-asserted-by":"crossref","first-page":"5126","DOI":"10.1021\/acs.analchem.4c06375","article-title":"Enhancing the accuracy of identification and relative quantification of unsaturated fatty acids in serum via a stable isotope-labeled double derivatization strategy","volume":"97","author":"Yuan","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib28","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1021\/acs.analchem.3c04320","article-title":"Intramolecular ring-chain equilibrium elimination strategy for pinpointing C=C positional and geometric isomers of N-Alkylpyridinium unsaturated fatty acid derivatives via ion mobility-mass spectrometry","volume":"96","author":"Li","year":"2024","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib29","doi-asserted-by":"crossref","first-page":"11189","DOI":"10.1021\/acs.analchem.4c00410","article-title":"Efficient strategy for characterization and quantification of polyunsaturated lipids by microwave-assisted MMPP epoxidation","volume":"96","author":"Huang","year":"2024","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib30","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.aca.2017.08.009","article-title":"Derivatization enhanced separation and sensitivity of long chain-free fatty acids: application to asthma using targeted and non-targeted liquid chromatography-mass spectrometry approach","volume":"989","author":"Bian","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2026.345133_bib31","doi-asserted-by":"crossref","first-page":"8197","DOI":"10.1021\/acs.analchem.2c05558","article-title":"Novel isotope-labeled derivatization strategy for the simultaneous analysis of fatty acids and fatty alcohols and its application in idiopathic inflammatory myopathies and pancreatic cancer","volume":"95","author":"Pu","year":"2023","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345133_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2023.125231","article-title":"Strategy for characterization and quantification of fatty acids in plasma by parallel d0\/d6-dansylhydrazine derivatization combined with UPLC-MS\/MS","volume":"267","author":"Li","year":"2024","journal-title":"Talanta"},{"key":"10.1016\/j.aca.2026.345133_bib33","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41577-021-00549-4","article-title":"Immunobiology and pathogenesis of hepatitis B virus infection","volume":"22","author":"Iannacone","year":"2022","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.aca.2026.345133_bib34","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1038\/s41423-025-01313-7","article-title":"Inflammation and immunity in liver homeostasis and disease: a nexus of hepatocytes, nonparenchymal cells and immune cells","volume":"22","author":"Kostallari","year":"2025","journal-title":"Cell. Mol. Immunol."},{"key":"10.1016\/j.aca.2026.345133_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2021.111315","article-title":"Adipose tissue and insulin resistance in obese","volume":"137","author":"Ahmed","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.aca.2026.345133_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.yexcr.2023.113721","article-title":"HBx induced upregulation of FATP2 promotes the development of hepatic lipid accumulation","volume":"430","author":"Lu","year":"2023","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.aca.2026.345133_bib37","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.jhep.2007.06.021","article-title":"Expression of hepatitis B virus proteins in transgenic mice alters lipid metabolism and induces oxidative stress in the liver","volume":"48","author":"Yang","year":"2008","journal-title":"J. Hepatol."},{"key":"10.1016\/j.aca.2026.345133_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2025.146856","article-title":"Fatty acid transport protein 2: a novel therapeutic target in lipid metabolism and disease - a review","volume":"322","author":"Pandi","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.aca.2026.345133_bib39","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1111\/1348-0421.13219","article-title":"Transcriptome profiling and viral-human interactome insights into HBV-driven oncogenic alterations in hepatocellular carcinoma","volume":"69","author":"Ananthakrishnan","year":"2025","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.aca.2026.345133_bib40","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1186\/s13024-024-00778-w","article-title":"Stearoyl-CoA desaturase-1: a potential therapeutic target for neurological disorders","volume":"19","author":"Loix","year":"2024","journal-title":"Mol. Neurodegener."},{"key":"10.1016\/j.aca.2026.345133_bib41","doi-asserted-by":"crossref","first-page":"356","DOI":"10.3390\/nu12020356","article-title":"Biological role of unsaturated fatty acid desaturases in health and disease","volume":"12","author":"Czumaj","year":"2020","journal-title":"Nutrients"},{"key":"10.1016\/j.aca.2026.345133_bib42","doi-asserted-by":"crossref","first-page":"4554","DOI":"10.1021\/jp502434t","article-title":"Radical-induced cis-trans isomerization of fatty acids: a theoretical study","volume":"118","author":"Tzeng","year":"2014","journal-title":"J. Phys. Chem. A"}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267026000838?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267026000838?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:49:40Z","timestamp":1777394980000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267026000838"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":42,"alternative-id":["S0003267026000838"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2026.345133","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Double derivatization-enhanced LC-MS\/MS for isomer-resolved identification and quantification of fatty acids","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2026.345133","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"345133"}}