{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:17:18Z","timestamp":1788236238439,"version":"build-2803163510"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100005064","name":"Hebei Province Science and Technology Support Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005064","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017696","name":"Hebei Province Graduate Innovation Funding Project","doi-asserted-by":"publisher","award":["CXZZBS2025068"],"award-info":[{"award-number":["CXZZBS2025068"]}],"id":[{"id":"10.13039\/501100017696","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.aca.2026.345503","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T16:17:23Z","timestamp":1775578643000},"page":"345503","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Simulated construction of tilmicosin nucleic acid aptamers based on molecular docking and molecular dynamics techniques"],"prefix":"10.1016","volume":"1406","author":[{"given":"Hongyu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaqing","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiying","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1795-3452","authenticated-orcid":false,"given":"Kun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2026.345503_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2020.08.080","article-title":"Transcriptomic investigation reveals toxic damage due to tilmicosin and potential resistance against tilmicosin in primary chicken myocardial cells","author":"Zhang","year":"2020","journal-title":"Poult. Sci."},{"key":"10.1016\/j.aca.2026.345503_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2024.140009","article-title":"A highly sensitive electrochemical immunosensor based on rGO-PEI-Ag nanocomposites for the detection of tilmicosin","volume":"461","author":"Chen","year":"2024","journal-title":"Food Chem."},{"key":"10.1016\/j.aca.2026.345503_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.mex.2024.102616","article-title":"Application of salting-out assisted liquid-liquid extraction for the determination of oxytetracycline, tetracycline, tilmicosin, and tylosin in cow milk by liquid chromatography with photodiode array detection","volume":"12","author":"Hassan","year":"2024","journal-title":"MethodsX"},{"issue":"2","key":"10.1016\/j.aca.2026.345503_bib4","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1016\/j.foodchem.2011.11.029","article-title":"Simultaneous separate and group determination of tylosin and tilmicosin in foodstuffs using single antibody-based immunoassay","volume":"132","author":"Burkin","year":"2011","journal-title":"Food Chem."},{"issue":"4","key":"10.1016\/j.aca.2026.345503_bib5","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1111\/jvp.12847","article-title":"Comparison of the pharmacokinetics of tilmicosin in plasma and lung tissue in healthy chickens and chickens experimentally infected with Mycoplasma gallisepticum","volume":"43","author":"Zhang","year":"2020","journal-title":"J. Vet. Pharmacol. Therapeut."},{"issue":"3","key":"10.1016\/j.aca.2026.345503_bib6","doi-asserted-by":"crossref","first-page":"662","DOI":"10.14202\/vetworld.2022.662-671","article-title":"The public health issue of antibiotic residues in food and feed: causes, consequences, and potential solutions","volume":"15","author":"Ars\u00e8ne","year":"2022","journal-title":"Vet. World"},{"key":"10.1016\/j.aca.2026.345503_bib7","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/nrmicro3270","article-title":"Microbiological effects of sublethal levels of antibiotics","volume":"12","author":"Andersson","year":"2014","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.aca.2026.345503_bib8","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.jchromb.2017.04.006","article-title":"Analysis of sulfonamides, tilmicosin and avermectins residues in typical animal matrices with multi-plug filtration cleanup by liquid chromatography-tandem mass spectrometry detection","volume":"1053","author":"Qin","year":"2017","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.aca.2026.345503_bib9","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1007\/s11947-024-03571-1","article-title":"Transfer of some macrolide group antibiotics from spiked milk to melting cheese and determination of their processing factor with LC\u2013MS\/MS","volume":"18","author":"\u00fclk\u00fc","year":"2024","journal-title":"Food Bioprocess Technol."},{"issue":"1","key":"10.1016\/j.aca.2026.345503_bib10","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1289\/ehp.02110s113","article-title":"Proteomic approaches to characterize protein modifications: new tools to study the effects of environmental exposures","volume":"110","author":"Liebler","year":"2002","journal-title":"Environ. Health Perspect."},{"issue":"10","key":"10.1016\/j.aca.2026.345503_bib11","doi-asserted-by":"crossref","first-page":"2511","DOI":"10.3390\/ijms20102511","article-title":"Updates on aptamer research","volume":"20","author":"Ali","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.aca.2026.345503_bib12","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.addr.2018.04.007","volume":"134","author":"Pascal","year":"2018","journal-title":"Aptamer chemistry. Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.aca.2026.345503_bib13","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.addr.2018.04.007","volume":"134","author":"R\u00f6thlisberger","year":"2018","journal-title":"Aptamer chemistry. Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.aca.2026.345503_bib14","article-title":"Advances of aptamer-based small-molecules sensors in body fluids detection","author":"Chen","year":"2023","journal-title":"Talanta"},{"issue":"11","key":"10.1016\/j.aca.2026.345503_bib15","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.3390\/foods13111749","article-title":"Advances in aptamer-based conjugate recognition techniques for the detection of small molecules in food","volume":"13","author":"Deng","year":"2024","journal-title":"Foods"},{"issue":"1","key":"10.1016\/j.aca.2026.345503_bib16","doi-asserted-by":"crossref","first-page":"29","DOI":"10.3390\/bios15010029","article-title":"Research progress in small-molecule detection using aptamer-based SERS techniques","volume":"15","author":"Zheng","year":"2025","journal-title":"Biosensors"},{"key":"10.1016\/j.aca.2026.345503_bib17","article-title":"Selection of aptamers targeting small molecules by capillary electrophoresis: advances, challenges, and prospects","volume":"78","author":"Hu","year":"2024","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.aca.2026.345503_bib18","doi-asserted-by":"crossref","DOI":"10.3390\/ijms20184331","article-title":"Molecular docking: shifting paradigms in drug discovery","author":"Pinzi","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.aca.2026.345503_bib19","doi-asserted-by":"crossref","DOI":"10.1039\/C6ME00083E","article-title":"Insights from molecular dynamics simulations for computational protein design","author":"Childers","year":"2017","journal-title":"Mol. Syst. Des. Eng."},{"key":"10.1016\/j.aca.2026.345503_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2024.135789","article-title":"In vitro selection and engineering azithromycin-specific aptamers and construction of a ratiometric fluorescent aptasensor for sensitive detection of azithromycin","volume":"411","author":"Huang","year":"2024","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.aca.2026.345503_bib21","doi-asserted-by":"crossref","DOI":"10.1021\/acs.jcim.2c00734","article-title":"Prediction of Aptamer\u2013small-molecule interactions using metastable states from multiple independent molecular dynamics simulations","author":"Rodr\u00edguez Serrano","year":"2022","journal-title":"J. Chem. Inf. Model."},{"key":"10.1016\/j.aca.2026.345503_bib22","article-title":"Dopamine interaction with DNA\/RNA aptamers: molecular dynamics simulation","author":"Sharifian","year":"2024","journal-title":"Comput. Theor. Chem."},{"key":"10.1016\/j.aca.2026.345503_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114562","article-title":"De novo design of DNA aptamers that target okadaic acid (OA) by docking-then-assembling of single nucleotides","author":"Song","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"D1","key":"10.1016\/j.aca.2026.345503_bib24","doi-asserted-by":"crossref","first-page":"D1516","DOI":"10.1093\/nar\/gkae1059","article-title":"PubChem 2025 update","volume":"53","author":"Kim","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2026.345503_bib25","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: high performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1\u20132","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"issue":"13","key":"10.1016\/j.aca.2026.345503_bib26","doi-asserted-by":"crossref","first-page":"3406","DOI":"10.1093\/nar\/gkg595","article-title":"Michael mfold web server for nucleic acid folding and hybridization prediction","volume":"31","author":"Zuker","year":"2003","journal-title":"Nucleic Acids Res."},{"issue":"14","key":"10.1016\/j.aca.2026.345503_bib27","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gks339","article-title":"Automated 3D structure composition for large RNAs","volume":"40","author":"Popenda","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2026.345503_bib28","doi-asserted-by":"crossref","DOI":"10.1002\/jcc.21256","article-title":"AutoDock4 and AutoDockTools4: automated docking with selective receptor flexibility","author":"Morris","year":"2009","journal-title":"J. Comput. Chem."},{"issue":"9","key":"10.1016\/j.aca.2026.345503_bib29","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1002\/jcc.20035","article-title":"Development and testing of a general amber force field","volume":"25","author":"Wang","year":"2004","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.aca.2026.345503_bib30","article-title":"Application and evaluation of molecular docking for aptamer and small molecular interaction - a case study with tetracycline antibiotics","author":"Liang","year":"2023","journal-title":"Talanta"},{"key":"10.1016\/j.aca.2026.345503_bib31","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.ijar.2016.09.012","article-title":"An extended depth-first search algorithm for optimal triangulation of Bayesian networks","volume":"80","author":"Li","year":"2017","journal-title":"Int. J. Approx. Reason."},{"key":"10.1016\/j.aca.2026.345503_bib32","doi-asserted-by":"crossref","first-page":"7779","DOI":"10.1007\/s00521-022-08086-4","article-title":"A multi-view clustering algorithm for attributed weighted multi-edge directed networks","volume":"35","author":"Khameneh","year":"2022","journal-title":"Neural Comput. Appl."},{"key":"10.1016\/j.aca.2026.345503_bib33","article-title":"Advancement of doxorubicin monitoring with a DNA fragmentation strategy for SYBR green I-based aptamer biosensors","author":"Wei","year":"2025","journal-title":"Talanta"},{"key":"10.1016\/j.aca.2026.345503_bib34","article-title":"Probing the ligand exchange kinetics of phenynyl-based ligands on colloidal Au nanoparticles","author":"Xiang","year":"2020","journal-title":"Mater. Chem. Front."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267026004538?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267026004538?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:01:48Z","timestamp":1777489308000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267026004538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":34,"alternative-id":["S0003267026004538"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2026.345503","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Simulated construction of tilmicosin nucleic acid aptamers based on molecular docking and molecular dynamics techniques","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2026.345503","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"345503"}}