{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T17:18:19Z","timestamp":1783099099485,"version":"3.54.6"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22074105"],"award-info":[{"award-number":["22074105"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["14DZ2261100"],"award-info":[{"award-number":["14DZ2261100"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.aca.2026.345588","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T15:05:43Z","timestamp":1777043143000},"page":"345588","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Amino-blocking sialic acid linkage specific alkylamidation for qualitative and quantitative analysis of sialylated glycoproteins"],"prefix":"10.1016","volume":"1409","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3254-9520","authenticated-orcid":false,"given":"Wanwei","family":"Luo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2877-8282","authenticated-orcid":false,"given":"Zhixin","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2026.345588_bib1","doi-asserted-by":"crossref","unstructured":"X. Wu, M. Xu, M. Geng, S. Chen, P.J. Little, S. Xu, J. Weng, Targeting protein modifications in metabolic diseases: molecular mechanisms and targeted therapies, (n.d.). https:\/\/doi.org\/10.1038\/s41392-023-01439-y.","DOI":"10.1038\/s41392-023-01439-y"},{"key":"10.1016\/j.aca.2026.345588_bib2","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1042\/BCJ20170111","article-title":"Role of glycosylation in nucleating protein folding and stability","volume":"474","author":"Jayaprakash","year":"2017","journal-title":"Biochem. J."},{"key":"10.1016\/j.aca.2026.345588_bib3","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1007\/s10719-012-9386-1","article-title":"Potential roles of N-glycosylation in cell adhesion","volume":"29","author":"Gu","year":"2012","journal-title":"Glycoconj. J."},{"key":"10.1016\/j.aca.2026.345588_bib4","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.ceb.2011.05.001","article-title":"Glycosylation, galectins and cellular signaling","volume":"23","author":"Boscher","year":"2011","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.aca.2026.345588_bib5","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.imlet.2020.11.003","article-title":"Progress in research into the role of abnormal glycosylation modification in tumor immunity","volume":"229","author":"min Liu","year":"2021","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.aca.2026.345588_bib6","doi-asserted-by":"crossref","first-page":"761","DOI":"10.3390\/medicina55120761","article-title":"Increase of MAL-II binding Alpha2,3-Sialylated glycan is associated with 5-FU resistance and short survival of cholangiocarcinoma patients","volume":"55","author":"Wattanavises","year":"2019","journal-title":"Medicina"},{"key":"10.1016\/j.aca.2026.345588_bib7","doi-asserted-by":"crossref","first-page":"6885","DOI":"10.1111\/jcmm.14572","article-title":"Truncated O-glycans promote epithelial-to-mesenchymal transition and stemness properties of pancreatic cancer cells","volume":"23","author":"Thomas","year":"2019","journal-title":"J. Cell Mol. Med."},{"key":"10.1016\/j.aca.2026.345588_bib8","doi-asserted-by":"crossref","first-page":"4679","DOI":"10.1111\/febs.13530","article-title":"The HIV glycan shield as a target for broadly neutralizing antibodies","volume":"282","author":"Doores","year":"2015","journal-title":"FEBS J."},{"key":"10.1016\/j.aca.2026.345588_bib9","doi-asserted-by":"crossref","first-page":"2500","DOI":"10.1128\/JVI.72.3.2500-2504.1998","article-title":"Human immunodeficiency virus (HIV) envelope binds to CXCR4 independently of CD4, and binding can be enhanced by interaction with soluble CD4 or by HIV envelope deglycosylation","volume":"72","author":"Bandres","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/j.aca.2026.345588_bib10","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1042\/bj1950639","article-title":"The role of the hydroxy amino acid in the triplet sequence Asn-Xaa-Thr(Ser) for the N-glycosylation step during glycoprotein biosynthesis","volume":"195","author":"Bause","year":"1981","journal-title":"Biochem. J."},{"key":"10.1016\/j.aca.2026.345588_bib11","doi-asserted-by":"crossref","DOI":"10.1186\/s13062-016-0137-2","article-title":"The multiple evolutionary origins of the eukaryotic N-glycosylation pathway","volume":"11","author":"Lombard","year":"2016","journal-title":"Biol. Direct"},{"key":"10.1016\/j.aca.2026.345588_bib12","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.202400057","article-title":"Recent advances in labeling-based quantitative glycomics: from high-throughput quantification to structural elucidation","volume":"25","author":"Wang","year":"2025","journal-title":"Proteomics"},{"key":"10.1016\/j.aca.2026.345588_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13045-024-01532-x","article-title":"Decoding the glycoproteome: a new frontier for biomarker discovery in cancer","volume":"17","author":"He","year":"2024","journal-title":"J. Hematol. Oncol."},{"key":"10.1016\/j.aca.2026.345588_bib14","doi-asserted-by":"crossref","first-page":"523","DOI":"10.2183\/pjab.95.036","article-title":"Sialic acid derivatization for glycan analysis by mass spectrometry","volume":"95","author":"Nishikaze","year":"2019","journal-title":"Proceedings of the Japan Academy, Series B"},{"key":"10.1016\/j.aca.2026.345588_bib15","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1007\/s00253-010-2700-6","article-title":"Biotechnological production and applications of N-acetyl-d-neuraminic acid: current state and perspectives","volume":"87","author":"Tao","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.aca.2026.345588_bib16","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1146\/annurev-immunol-020711-075018","article-title":"Siglecs and immune regulation","volume":"30","author":"Pillai","year":"2012","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.aca.2026.345588_bib17","first-page":"2817","article-title":"Sialic acid utilization by bacterial pathogens","volume":"153","author":"Severi","year":"2007","journal-title":"Microbiology (N. Y.)"},{"key":"10.1016\/j.aca.2026.345588_bib18","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.foodchem.2012.08.078","article-title":"Gangliosides and sialic acid effects upon newborn pathogenic bacteria adhesion: an in vitro study","volume":"136","author":"Salcedo","year":"2013","journal-title":"Food Chem."},{"key":"10.1016\/j.aca.2026.345588_bib19","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1093\/glycob\/cwy061","article-title":"Biophysical analysis of sialic acid recognition by the complement regulator factor H","volume":"28","author":"Schmidt","year":"2018","journal-title":"Glycobiology"},{"key":"10.1016\/j.aca.2026.345588_bib20","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.cbpa.2021.01.001","article-title":"Siglec and anti-siglec therapies","volume":"62","author":"Murugesan","year":"2021","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.aca.2026.345588_bib21","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/s41570-020-0174-3","article-title":"Glycomics studies using sialic acid derivatization and mass spectrometry","volume":"4","author":"de Haan","year":"2020","journal-title":"Nat. Rev. Chem"},{"key":"10.1016\/j.aca.2026.345588_bib22","doi-asserted-by":"crossref","first-page":"5784","DOI":"10.1021\/ac500335t","article-title":"High-throughput profiling of protein N - Glycosylation by MALDI- TOF-MS employing linkage-specific sialic acid esterification","volume":"86","author":"Reiding","year":"2014","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345588_bib23","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.bbagen.2019.03.001","article-title":"Serum protein N-glycosylation changes in multiple myeloma","volume":"1863","author":"Zhang","year":"2019","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"10.1016\/j.aca.2026.345588_bib24","doi-asserted-by":"crossref","first-page":"30610","DOI":"10.18632\/oncotarget.25753","article-title":"Serum N -glycome alterations in colorectal cancer associate with survival","volume":"9","author":"de Vroome","year":"2018","journal-title":"Oncotarget"},{"key":"10.1016\/j.aca.2026.345588_bib25","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1002\/rcm.3867","article-title":"Derivatization of sialic acids for stabilization in matrix-assisted laser desorption\/ionization mass spectrometry and concomitant differentiation of \u03b1(2 \u2192 3)- and \u03b1(2 \u2192 6)-isomers","volume":"23","author":"Wheeler","year":"2009","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"10.1016\/j.aca.2026.345588_bib26","doi-asserted-by":"crossref","first-page":"2353","DOI":"10.1021\/acs.analchem.6b04150","article-title":"Differentiation of sialyl linkage isomers by one-pot sialic acid derivatization for mass spectrometry-based glycan profiling","volume":"89","author":"Nishikaze","year":"2017","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345588_bib27","doi-asserted-by":"crossref","first-page":"8300","DOI":"10.1021\/ac101831t","article-title":"Methylamidation for sialoglycomics by MALDI-MS: a facile derivatization strategy for both \u03b12,3- and \u03b12,6-linked sialic acids","volume":"82","author":"Liu","year":"2010","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345588_bib28","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.ab.2018.11.014","article-title":"Quantitative LC-MS and MS\/MS analysis of sialylated glycans modified by linkage-specific alkylamidation","volume":"567","author":"Suzuki","year":"2019","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.aca.2026.345588_bib29","doi-asserted-by":"crossref","first-page":"8284","DOI":"10.1021\/acs.analchem.5b02426","article-title":"Linkage-specific sialic acid derivatization for MALDI-TOF-MS profiling of IgG glycopeptides","volume":"87","author":"De Haan","year":"2015","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345588_bib30","doi-asserted-by":"crossref","first-page":"13343","DOI":"10.1021\/acs.analchem.9b03617","article-title":"Comparative glycomic analysis of sialyl linkage isomers by sialic acid linkage-specific alkylamidation in combination with stable isotope labeling of \u03b12,3-Linked sialic acid residues","volume":"91","author":"Hanamatsu","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345588_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2022.340391","article-title":"Sialic acid linkage-specific quantitative N-glycoproteomics using selective alkylamidation and multiplex TMT-labeling","volume":"1230","author":"Yang","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2026.345588_bib32","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.aca.2016.04.023","article-title":"MALDI-MS analysis of sialylated N-glycan linkage isomers using solid-phase two step derivatization method","volume":"924","author":"Li","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2026.345588_bib33","doi-asserted-by":"crossref","first-page":"13193","DOI":"10.1021\/acs.analchem.8b02775","article-title":"Sialic acid linkage specific derivatization of glycosphingolipid glycans by ring-opening aminolysis of lactones","volume":"90","author":"Hanamatsu","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345588_bib34","doi-asserted-by":"crossref","first-page":"5763","DOI":"10.1021\/acs.analchem.0c05149","article-title":"Derivatization of sialylated glycopeptides (DOSG) enabling site-specific isomeric profiling using LC-MS\/MS","volume":"93","author":"Zhong","year":"2021","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345588_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2022.340492","article-title":"Derivatization of sialylated glycopeptides plus based sialoglycopeptides enrichment using cation exchange media","volume":"1233","author":"Zhong","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2026.345588_bib36","doi-asserted-by":"crossref","first-page":"15993","DOI":"10.1021\/acs.analchem.9b04727","article-title":"Stable isotope sequential derivatization for linkage-specific analysis of sialylated N-Glycan isomers by MS","volume":"91","author":"Peng","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345588_bib37","article-title":"A high-throughput N-glycan analysis strategy with targeted mass spectrometry (HTnGQs-target) for liver disease diagnosis","author":"Liu","year":"2025","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2026.345588_bib38","doi-asserted-by":"crossref","first-page":"9590","DOI":"10.1039\/D1CC04142H","article-title":"Linkage-selective derivatization for glycosylation site- and glycoform-specific characterization of sialic acid isomers using mass spectrometry","volume":"57","author":"Peng","year":"2021","journal-title":"Chem. Commun."},{"key":"10.1016\/j.aca.2026.345588_bib39","doi-asserted-by":"crossref","DOI":"10.1093\/nsr\/nwac059","article-title":"High-throughput site-specific N-glycoproteomics reveals glyco-signatures for liver disease diagnosis","volume":"10","author":"Sun","year":"2023","journal-title":"Natl. Sci. Rev."},{"issue":"12","key":"10.1016\/j.aca.2026.345588_bib40","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1038\/s41592-021-01306-0","article-title":"Precise, fast and comprehensive analysis of intact glycopeptides and modified glycans with pGlyco3","volume":"18","author":"Zeng","year":"2021","journal-title":"Nat. Methods"},{"issue":"1","key":"10.1016\/j.aca.2026.345588_bib41","doi-asserted-by":"crossref","first-page":"7539","DOI":"10.1038\/s41467-022-35172-x","article-title":"pGlycoQuant with a deep residual network for quantitative glycoproteomics at intact glycopeptide level","volume":"13","author":"Kong","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2026.345588_bib42","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2020.121359","article-title":"Quantitative N-glycoproteomics using stable isotopic diethyl labeling","volume":"219","author":"Wang","year":"2020","journal-title":"Talanta"},{"key":"10.1016\/j.aca.2026.345588_bib43","doi-asserted-by":"crossref","first-page":"D1211","DOI":"10.1093\/nar\/gky869","article-title":"iProX: an integrated proteome resource","volume":"47","author":"Ma","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2026.345588_bib44","doi-asserted-by":"crossref","first-page":"D1522","DOI":"10.1093\/nar\/gkab1081","article-title":"iProX in 2021: connecting proteomics data sharing with big data","volume":"50","author":"Chen","year":"2022","journal-title":"Nucleic Acids Res."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267026005386?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267026005386?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T16:26:10Z","timestamp":1783095970000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267026005386"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":44,"alternative-id":["S0003267026005386"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2026.345588","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Amino-blocking sialic acid linkage specific alkylamidation for qualitative and quantitative analysis of sialylated glycoproteins","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2026.345588","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"345588"}}