{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T09:25:10Z","timestamp":1787131510893,"version":"build-2736575974"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Hebei Provincial Natural Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017959","name":"Hebei Province Health Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017959","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.aca.2026.345903","type":"journal-article","created":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T14:16:22Z","timestamp":1782656182000},"page":"345903","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Reduction-Alkylation Proteome Profiling (RAPP): A novel strategy for proteome-wide target identification"],"prefix":"10.1016","volume":"1419","author":[{"given":"Yiying","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwen","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuran","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangyu","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panpan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dezhi","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2026.345903_bib1","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1093\/bib\/bbz122","article-title":"An omics perspective on drug target discovery platforms","volume":"21","author":"Paananen","year":"2020","journal-title":"Briefings Bioinf."},{"key":"10.1016\/j.aca.2026.345903_bib2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1039\/C4SC02339K","article-title":"Target discovery of acivicin in cancer cells elucidates its mechanism of growth inhibition","volume":"6","author":"Kreuzer","year":"2015","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2026.345903_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.jchromb.2020.122332","article-title":"Affinity chromatography: a review of trends and developments over the past 50 years","volume":"1157","author":"Rodriguez","year":"2020","journal-title":"J. Chromatogr., B: Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.aca.2026.345903_bib4","doi-asserted-by":"crossref","first-page":"21208","DOI":"10.1021\/acs.jmedchem.4c02019","article-title":"Covalent inhibitors of KEAP1 with exquisite selectivity","volume":"67","author":"Fejes","year":"2024","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.aca.2026.345903_bib5","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1038\/s41570-020-00229-2","article-title":"Exploring new targets and chemical space with affinity selection-mass spectrometry","volume":"5","author":"Prudent","year":"2021","journal-title":"Nat. Rev. Chem"},{"key":"10.1016\/j.aca.2026.345903_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2019.06.024","article-title":"Modification-free approaches to screen drug targets at proteome level","volume":"124","author":"Lyu","year":"2020","journal-title":"Trac. Trends Anal. Chem."},{"key":"10.1016\/j.aca.2026.345903_bib7","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1038\/s41589-023-01355-w","article-title":"Chemoproteomic mapping of the glycolytic targetome in cancer cells","volume":"19","author":"Tian","year":"2023","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.aca.2026.345903_bib8","doi-asserted-by":"crossref","DOI":"10.1038\/s41592-024-02553-7","article-title":"A peptide-centric local stability assay enables proteome-scale identification of the protein targets and binding regions of diverse ligands","volume":"22","author":"Li","year":"2025","journal-title":"Nat. Methods"},{"key":"10.1016\/j.aca.2026.345903_bib9","doi-asserted-by":"crossref","first-page":"2100","DOI":"10.1038\/nprot.2014.138","article-title":"The cellular thermal shift assay for evaluating drug target interactions in cells","volume":"9","author":"Jafari","year":"2014","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.aca.2026.345903_bib10","doi-asserted-by":"crossref","first-page":"9249","DOI":"10.1021\/acs.analchem.8b01772","article-title":"Chemical denaturation and protein precipitation approach for discovery and quantitation of protein-drug interactions","volume":"90","author":"Meng","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345903_bib11","doi-asserted-by":"crossref","first-page":"12403","DOI":"10.1039\/D2SC03326G","article-title":"Highly effective identification of drug targets at the proteome level by pH-dependent protein precipitation","volume":"13","author":"Zhang","year":"2022","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2026.345903_bib12","doi-asserted-by":"crossref","first-page":"4175","DOI":"10.1021\/ac702610a","article-title":"Thermodynamic analysis of protein stability and ligand binding using a chemical modification- and mass-spectrometry based strategy","volume":"80","author":"West","year":"2008","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.345903_bib13","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.tibs.2020.05.006","article-title":"Detecting protein-small molecule interactions using limited proteolysis-mass spectrometry (LiP-MS)","volume":"45","author":"Pepelnjak","year":"2020","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.aca.2026.345903_bib14","doi-asserted-by":"crossref","first-page":"4207","DOI":"10.1021\/bi992922o","article-title":"Disulfide bonds and protein folding","volume":"39","author":"Wedemeyer","year":"2000","journal-title":"Biochemistry"},{"key":"10.1016\/j.aca.2026.345903_bib15","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0301-4622(01)00228-9","article-title":"Evidence of reduced flexibility in disulfide bridge-depleted azurin: a molecular dynamics simulation study","volume":"94","author":"Rizzuti","year":"2001","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.aca.2026.345903_bib16","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1038\/nprot.2015.101","article-title":"Thermal proteome profiling for unbiased identification of direct and indirect drug targets using multiplexed quantitative mass spectrometry","volume":"10","author":"Franken","year":"2015","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.aca.2026.345903_bib17","author":"Childs"},{"key":"10.1016\/j.aca.2026.345903_bib18","doi-asserted-by":"crossref","first-page":"16942","DOI":"10.1074\/jbc.RA119.009742","article-title":"Binding-induced folding under unfolding conditions: switching between induced fit and conformational selection mechanisms","volume":"294","author":"Sen","year":"2019","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.aca.2026.345903_bib19","doi-asserted-by":"crossref","DOI":"10.1038\/srep38572","article-title":"Reactivity of disulfide bonds is markedly affected by structure and environment: implications for protein modification and stability","volume":"6","author":"Karimi","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.aca.2026.345903_bib20","doi-asserted-by":"crossref","first-page":"83","DOI":"10.3390\/antib12040083","article-title":"Heterogeneity in disulfide bond reduction in IgG1 antibodies is governed by solvent accessibility of the cysteines","volume":"12","author":"Natesan","year":"2023","journal-title":"Antibodies"},{"key":"10.1016\/j.aca.2026.345903_bib21","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1038\/s41556-023-01091-2","article-title":"Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis","volume":"25","author":"Liu","year":"2023","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.aca.2026.345903_bib22","doi-asserted-by":"crossref","first-page":"24550","DOI":"10.1016\/S0021-9258(17)31426-6","article-title":"Chaperone-like activity of protein disulfide isomerase in the refolding of a protein with no disulfide bonds","volume":"269","author":"Cai","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.aca.2026.345903_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2024.122797","article-title":"Characterization of the mechanisms governing heparin-bovine serum albumin complex interactions and associated anticoagulant activity","volume":"348","author":"Zhang","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.aca.2026.345903_bib24","doi-asserted-by":"crossref","DOI":"10.1038\/s42003-020-0795-6","article-title":"An isothermal shift assay for proteome scale drug-target identification","volume":"3","author":"Ball","year":"2020","journal-title":"Commun. Biol."},{"key":"10.1016\/j.aca.2026.345903_bib25","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cellsig.2005.04.007","article-title":"Essential role of the LIM domain in the formation of the PKC\u03b5-ENH-N-type Ca2+ channel complex","volume":"18","author":"Chen","year":"2006","journal-title":"Cell. Signal."},{"key":"10.1016\/j.aca.2026.345903_bib26","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0197635","article-title":"Structural study reveals the temperature-dependent conformational flexibility of Tk-PTP, a protein tyrosine phosphatase from Thermococcus kodakaraensis KOD1","volume":"13","author":"Yun","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.aca.2026.345903_bib27","doi-asserted-by":"crossref","first-page":"6755","DOI":"10.1074\/jbc.M114.609305","article-title":"Identification of FAH domain-containing protein 1 (FAHD1) as oxaloacetate decarboxylase","volume":"290","author":"Pircher","year":"2015","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.aca.2026.345903_bib28","doi-asserted-by":"crossref","DOI":"10.3390\/molecules26165009","article-title":"Inhibitors of fumarylacetoacetate hydrolase domain containing protein 1 (FAHD1)","volume":"26","author":"Weiss","year":"2021","journal-title":"Molecules"},{"key":"10.1016\/j.aca.2026.345903_bib29","doi-asserted-by":"crossref","first-page":"34752","DOI":"10.1074\/jbc.M111.262790","article-title":"Orientation and cellular distribution of membrane-bound Catechol-O-methyltransferase in cortical neurons IMPLICATIONS FOR DRUG DEVELOPMENT","volume":"286","author":"Chen","year":"2011","journal-title":"J. Biol. Chem."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267026008536?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267026008536?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:32:26Z","timestamp":1787128346000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267026008536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":29,"alternative-id":["S0003267026008536"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2026.345903","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Reduction-Alkylation Proteome Profiling (RAPP): A novel strategy for proteome-wide target identification","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2026.345903","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"345903"}}