{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T09:09:06Z","timestamp":1789808946433,"version":"4.0.1"},"reference-count":23,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32371521"],"award-info":[{"award-number":["32371521"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["2023SHZDZX02D33"],"award-info":[{"award-number":["2023SHZDZX02D33"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003347","name":"Fudan University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.aca.2026.346071","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T15:15:42Z","timestamp":1786029342000},"page":"346071","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["UPCRISPR: an ultrafast, one-tube multiplexed nucleic acid detection system for resolving the diagnostic gray zone in HPV16\/18 detection"],"prefix":"10.1016","volume":"1422","author":[{"given":"Linlin","family":"Bai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianwei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8891-651X","authenticated-orcid":false,"given":"Weiqi","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiming","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingmin","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2945-2046","authenticated-orcid":false,"given":"Rui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.aca.2026.346071_bib1","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. Clin."},{"issue":"9916","key":"10.1016\/j.aca.2026.346071_bib2","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/S0140-6736(13)62218-7","article-title":"Efficacy of HPV-based screening for prevention of invasive cervical cancer: follow-up of four European randomised controlled trials","volume":"383","author":"Ronco","year":"2014","journal-title":"Lancet"},{"issue":"2","key":"10.1016\/j.aca.2026.346071_bib3","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1001\/jamaoncol.2020.6575","article-title":"Effectiveness of high-risk human papillomavirus testing for cervical cancer screening in China: a multicenter, open-label, randomized clinical trial","volume":"7","author":"Zhang","year":"2021","journal-title":"JAMA Oncol."},{"issue":"6","key":"10.1016\/j.aca.2026.346071_bib4","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.canep.2013.10.005","article-title":"Human papillomavirus genotype distribution in cervical intraepithelial neoplasia grades 1 or worse among 4215 Chinese women in a population-based study","volume":"37","author":"Zhang","year":"2013","journal-title":"Cancer Epidemiol"},{"issue":"8","key":"10.1016\/j.aca.2026.346071_bib5","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1158\/1078-0432.CCR-04-2367","article-title":"Detection of human papillomavirus infection of the cervix in very elderly women using PCR","volume":"11","author":"Takubo","year":"2005","journal-title":"Clin. Cancer Res."},{"issue":"16","key":"10.1016\/j.aca.2026.346071_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21165674","article-title":"Development and evaluation of novel and highly sensitive single-tube nested real-time RT-PCR assays for SARS-CoV-2 detection","volume":"21","author":"Yip","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.aca.2026.346071_bib7","doi-asserted-by":"crossref","first-page":"114","DOI":"10.34133\/bmef.0114","article-title":"Application of CRISPR-cas system in human papillomavirus detection using biosensor devices and point-of-care technologies","volume":"6","author":"He","year":"2025","journal-title":"BME Front."},{"key":"10.1016\/j.aca.2026.346071_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2023.117328","article-title":"Next-generation CRISPR-based diagnostic tools for human diseases","volume":"168","author":"Wang","year":"2023","journal-title":"Trends Anal. Chem."},{"issue":"1","key":"10.1016\/j.aca.2026.346071_bib9","article-title":"Design, validation, and implementation of multiplex digital PCR assays for simultaneous quantification of multiple targets","volume":"78","author":"de Korne-Elenbaas","year":"2025","journal-title":"Lett. Appl. Microbiol."},{"issue":"3","key":"10.1016\/j.aca.2026.346071_bib10","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s43657-024-00216-3","article-title":"MVPCR: Multiplex visual detection strategy based on ultrafast PCR for point-of-care pathogens detection within 10 min","volume":"5","author":"Zhang","year":"2025","journal-title":"Phenomics"},{"issue":"6387","key":"10.1016\/j.aca.2026.346071_bib11","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"issue":"10","key":"10.1016\/j.aca.2026.346071_bib12","doi-asserted-by":"crossref","first-page":"2986","DOI":"10.1038\/s41596-019-0210-2","article-title":"SHERLOCK: nucleic acid detection with CRISPR nucleases","volume":"14","author":"Kellner","year":"2019","journal-title":"Nat. Protoc."},{"issue":"1","key":"10.1016\/j.aca.2026.346071_bib13","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/s41421-018-0028-z","article-title":"CRISPR-Cas12a-assisted nucleic acid detection","volume":"4","author":"Li","year":"2018","journal-title":"Cell Discov."},{"issue":"10","key":"10.1016\/j.aca.2026.346071_bib14","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1021\/acssynbio.9b00209","article-title":"HOLMESv2: a CRISPR-Cas12b-Assisted platform for nucleic acid detection and DNA methylation quantitation","volume":"8","author":"Li","year":"2019","journal-title":"ACS Synth. Biol."},{"issue":"4","key":"10.1016\/j.aca.2026.346071_bib15","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1007\/s43657-025-00240-x","article-title":"SIMPLE: a one-pot RPA-Cas12b method for single nucleotide variations identification with an expanded scope and clinical applications","volume":"5","author":"Guo","year":"2025","journal-title":"Phenomics"},{"key":"10.1016\/j.aca.2026.346071_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112766","article-title":"opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-CoV-2 detection","volume":"172","author":"Wang","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2026.346071_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2025.344830","article-title":"A novel multiplex RPA\/CRISPR-Cas12a integrated biosensor for on-site detection of high-risk HPV genotypes","volume":"1382","author":"Bao","year":"2026","journal-title":"Anal. Chim. Acta"},{"issue":"19","key":"10.1016\/j.aca.2026.346071_bib18","doi-asserted-by":"crossref","first-page":"12156","DOI":"10.1021\/acs.analchem.9b01526","article-title":"Cas12aVDet: a CRISPR\/Cas12a-Based platform for rapid and visual nucleic acid detection","volume":"91","author":"Wang","year":"2019","journal-title":"Anal. Chem."},{"issue":"5","key":"10.1016\/j.aca.2026.346071_bib19","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1080\/14737159.2025.2486653","article-title":"Evolution and future of cervical cancer screening: from cytology to primary HPV testing and the impact of vaccination","volume":"25","author":"El-Zein","year":"2025","journal-title":"Expert Rev. Mol. Diagn."},{"issue":"7","key":"10.1016\/j.aca.2026.346071_bib20","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.1021\/acs.jafc.1c06715","article-title":"Rapid, visual, and sequence-specific detection of salmonella in egg liquid with vis-NEAA, a CRISPR\/Cas12 empowered new strategy","volume":"70","author":"Bai","year":"2022","journal-title":"J. Agric. Food Chem."},{"issue":"7","key":"10.1016\/j.aca.2026.346071_bib21","first-page":"3145","article-title":"Ultraspecific one-pot CRISPR-based \u201cgreen-yellow-red\u201d multiplex detection strategy integrated with portable cartridge for point-of-care diagnosis","volume":"96","author":"Chen","year":"2024","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.aca.2026.346071_bib22","doi-asserted-by":"crossref","first-page":"4711","DOI":"10.1038\/s41467-020-18575-6","article-title":"Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay","volume":"11","author":"Ding","year":"2020","journal-title":"Nat. Commun."},{"issue":"4","key":"10.1016\/j.aca.2026.346071_bib23","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.tibtech.2022.07.015","article-title":"Microfluidics: the propellant of CRISPR-based nucleic acid detection","volume":"41","author":"Chen","year":"2023","journal-title":"Trends Biotechnol."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267026010214?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267026010214?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T08:20:33Z","timestamp":1789806033000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267026010214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":23,"alternative-id":["S0003267026010214"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2026.346071","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"UPCRISPR: an ultrafast, one-tube multiplexed nucleic acid detection system for resolving the diagnostic gray zone in HPV16\/18 detection","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2026.346071","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"346071"}}