{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:54:08Z","timestamp":1791305648904,"version":"4.3.1"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U24A20751"],"award-info":[{"award-number":["U24A20751"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82372334"],"award-info":[{"award-number":["82372334"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002865","name":"Chongqing Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["2025MSXM040"],"award-info":[{"award-number":["2025MSXM040"]}],"id":[{"id":"10.13039\/501100002865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.aca.2026.346180","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:49:31Z","timestamp":1787755771000},"page":"346180","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["A proximity-allosteric Adapter unlocks Cas12a exponential amplification for versatile homogeneous assay"],"prefix":"10.1016","volume":"1423","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5315-8082","authenticated-orcid":false,"given":"Feiyu","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangling","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuxian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingping","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linguo","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanxin","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanna","family":"Ban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiantian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2026.346180_bib1","doi-asserted-by":"crossref","first-page":"8517","DOI":"10.1021\/acs.analchem.7b02229","article-title":"Homogeneous assays of second messenger signaling and hormone secretion using thermofluorimetric methods that minimize calibration burden","volume":"89","author":"Hu","year":"2017","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.346180_bib2","doi-asserted-by":"crossref","first-page":"4586","DOI":"10.1038\/s41467-021-24874-3","article-title":"A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays","volume":"12","author":"Ni","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2026.346180_bib3","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1038\/nmeth947","article-title":"Direct observation of individual endogenous protein complexes in situ by proximity ligation","volume":"3","author":"S\u00f6derberg","year":"2006","journal-title":"Nat. Methods"},{"key":"10.1016\/j.aca.2026.346180_bib4","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/nbt0502-473","article-title":"Protein detection using proximity-dependent DNA ligation assays","volume":"20","author":"Fredriksson","year":"2002","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.aca.2026.346180_bib5","doi-asserted-by":"crossref","first-page":"7238","DOI":"10.1038\/s41467-023-42834-x","article-title":"NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing","volume":"14","author":"Feng","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2026.346180_bib6","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2023.1188313","article-title":"Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules","volume":"11","author":"Park","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.aca.2026.346180_bib7","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202303034","article-title":"Asymmetric hairpins DNA encapsulated silver nanoclusters for in Situ fluorescence imaging of fusion gene isoforms in bone marrow","volume":"19","author":"Gou","year":"2023","journal-title":"Small"},{"key":"10.1016\/j.aca.2026.346180_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2021.100168","article-title":"Proximity extension assay in combination with next-generation sequencing for high-throughput proteome-wide analysis","volume":"20","author":"Wik","year":"2021","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.aca.2026.346180_bib9","doi-asserted-by":"crossref","first-page":"2928","DOI":"10.1021\/acssensors.1c00695","article-title":"One-Pot identification of BCR\/ABL transcript isoforms based on nanocluster beacon","volume":"6","author":"Gou","year":"2021","journal-title":"ACS Sens."},{"key":"10.1016\/j.aca.2026.346180_bib10","doi-asserted-by":"crossref","DOI":"10.1002\/smtd.202500194","article-title":"Gemini molecular assembly colocalization (GOAL): Accurate and efficient fusion genotyping for chronic myeloid leukemia intelligent diagnosis","volume":"9","author":"Huang","year":"2025","journal-title":"Small Methods"},{"key":"10.1016\/j.aca.2026.346180_bib11","doi-asserted-by":"crossref","first-page":"2825","DOI":"10.1021\/acs.analchem.5c05529","article-title":"Simultaneous detection of SARS-CoV-2 nucleocapsid protein and RNA by aptamer-based proximity ligation and quantitative PCR","volume":"98","author":"Zaripov","year":"2026","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.346180_bib12","doi-asserted-by":"crossref","first-page":"8890","DOI":"10.1021\/acs.analchem.5c00093","article-title":"T4 DNA polymerase-proofread DNA binding identifier for sensitive homogeneous immunoassays","volume":"97","author":"Guang","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.346180_bib13","doi-asserted-by":"crossref","first-page":"5035","DOI":"10.1038\/s41467-025-60265-8","article-title":"Spatially blocked split CRISPR-Cas12a system for ultra-sensitive and versatile small molecule activation and detection","volume":"16","author":"Hu","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2026.346180_bib14","doi-asserted-by":"crossref","first-page":"2692","DOI":"10.1021\/acs.analchem.3c05497","article-title":"Photoactivatable CRISPR\/Cas12a sensors for biomarkers imaging and point-of-care diagnostics","volume":"96","author":"Li","year":"2024","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.346180_bib15","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaf311","article-title":"Exploring the effect of activator topology on CRISPR-Cas12a trans-cleavage activity","volume":"53","author":"Zhu","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2026.346180_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2025.168333","article-title":"A novel vortexing-driven droplet digital CRISPR (VTD-CRISPR) platform for rapid and absolute quantitative detection of genetically modified crops","volume":"523","author":"Chi","year":"2025","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.aca.2026.346180_bib17","doi-asserted-by":"crossref","first-page":"7384","DOI":"10.1093\/nar\/gkae470","article-title":"Modular CRISPR\/Cas12a synergistic activation platform for detection and logic operations","volume":"52","author":"Hu","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2026.346180_bib18","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaf117","article-title":"Regulation of CRISPR trans-cleavage activity by an overhanging activator","volume":"53","author":"Yin","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2026.346180_bib19","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkag187","article-title":"A tailored phosphorothioate coordinator enables CRISPR\/Cas In-Situ amplification","volume":"54","author":"Yang","year":"2026","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2026.346180_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2026.139769","article-title":"Proximity-activated CRISPR nanomachine for molecular characterization of cancer cells","volume":"458","author":"Li","year":"2026","journal-title":"Sens. Actuators, B"},{"key":"10.1016\/j.aca.2026.346180_bib21","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1021\/acssynbio.4c00816","article-title":"Building the future of clinical diagnostics: an analysis of potential benefits and current barriers in CRISPR\/cas diagnostics","volume":"14","author":"Van Dongen","year":"2025","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.aca.2026.346180_bib22","doi-asserted-by":"crossref","first-page":"7442","DOI":"10.1021\/acs.analchem.2c01373","article-title":"CRISPR-Cas12a-Derived photoelectrochemical biosensor for point-of-care diagnosis of nucleic acid","volume":"94","author":"Zeng","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.346180_bib23","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1038\/s41467-024-46001-8","article-title":"Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA\/RNA sensing","volume":"15","author":"Deng","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2026.346180_bib24","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202516838","article-title":"Autocatalytic circular DNA powered plasmonic CRISPR\/Cas12a platform for ultrasensitive non-nucleic acid target sensing","volume":"65","author":"Yao","year":"2026","journal-title":"Angew. Chem., Int. Ed. Engl."},{"key":"10.1016\/j.aca.2026.346180_bib25","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2420166122","article-title":"Amplification-free, OR-Gated CRISPR-cascade reaction for pathogen detection in blood samples","volume":"122","author":"Lim","year":"2025","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.aca.2026.346180_bib26","doi-asserted-by":"crossref","first-page":"gkad953","DOI":"10.1093\/nar\/gkad953","article-title":"Rapid in situ RNA imaging based on Cas12a thrusting strand displacement reaction","volume":"51","author":"Cheng","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2026.346180_bib27","doi-asserted-by":"crossref","first-page":"11727","DOI":"10.1093\/nar\/gkac886","article-title":"A PAM-free CRISPR\/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration","volume":"50","author":"Wu","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2026.346180_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.ccr.2020.213624","article-title":"Phosphorothioate nucleic acids for probing metal binding, biosensing and nanotechnology","volume":"428","author":"Saran","year":"2021","journal-title":"Coord. Chem. Rev."},{"key":"10.1016\/j.aca.2026.346180_bib29","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1038\/s41467-025-66893-4","article-title":"Programmable argonaute-mediated single-nucleotide variant sequencing of cell-free DNA for multi-cancer early detection","volume":"17","author":"Guo","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2026.346180_bib30","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1158\/2767-9764.CRC-25-0329","article-title":"Molecular characterization of oncogenic gene fusions in a large real-world cohort of solid tumors","volume":"5","author":"Gai","year":"2025","journal-title":"Cancer Res. Commun."},{"key":"10.1016\/j.aca.2026.346180_bib31","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1002\/ajh.26642","article-title":"Chronic Myeloid leukemia: 2022 update on diagnosis, therapy, and monitoring","volume":"97","author":"Jabbour","year":"2022","journal-title":"Am. J. Hematol."},{"key":"10.1016\/j.aca.2026.346180_bib32","article-title":"Impact of BCR-ABL1 transcript type on outcome in chronic myeloid leukemia patients treated with tyrosine kinase inhibitors: a pairwise and bayesian network meta-analysis","volume":"12","author":"Chen","year":"2022","journal-title":"Front. Oncol."},{"key":"10.1016\/j.aca.2026.346180_bib33","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1038\/s43587-023-00403-3","article-title":"Blood biomarkers for Alzheimer's Disease in clinical practice and trials","volume":"3","author":"Hansson","year":"2023","journal-title":"Nat. Aging"},{"key":"10.1016\/j.aca.2026.346180_bib34","doi-asserted-by":"crossref","first-page":"2036","DOI":"10.1038\/s41591-025-03622-w","article-title":"Plasma Phospho-Tau217 for Alzheimer's Disease diagnosis in primary and decondary care using a fully automated platform","volume":"31","author":"Palmqvist","year":"2025","journal-title":"Nat. Med."},{"key":"10.1016\/j.aca.2026.346180_bib35","doi-asserted-by":"crossref","first-page":"26560","DOI":"10.1021\/acs.analchem.5c04856","article-title":"Homogeneous femtomolar detection of P-tau181 via proximity extension and CRISPR\/Cas technique","volume":"97","author":"Liu","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2026.346180_bib36","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1038\/s41591-026-04206-y","volume":"32","author":"Petersen","year":"2026","journal-title":"Nat. Med."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000326702601130X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000326702601130X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:11:51Z","timestamp":1791303111000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S000326702601130X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":36,"alternative-id":["S000326702601130X"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2026.346180","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A proximity-allosteric Adapter unlocks Cas12a exponential amplification for versatile homogeneous assay","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2026.346180","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"346180"}}