{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T16:21:26Z","timestamp":1782490886017,"version":"3.54.5"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81271860"],"award-info":[{"award-number":["81271860"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81141084"],"award-info":[{"award-number":["81141084"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Tropica"],"published-print":{"date-parts":[[2015,2]]},"DOI":"10.1016\/j.actatropica.2014.10.017","type":"journal-article","created":{"date-parts":[[2014,11,2]],"date-time":"2014-11-02T00:02:25Z","timestamp":1414886545000},"page":"57-63","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":69,"special_numbering":"C","title":["Characterization of a Trichinella spiralis 31kDa protein and its potential application for the serodiagnosis of trichinellosis"],"prefix":"10.1016","volume":"142","author":[{"given":"Jing","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge Ge","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li Na","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai Bing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo Dan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong Quan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.actatropica.2014.10.017_bib0005","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0166-6851(94)90072-8","article-title":"Molecular analysis of antigens targeted by protective antibodies in rapid expulsion of Trichinella spiralis","volume":"65","author":"Arasu","year":"1994","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0010","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1477-5956-10-10","article-title":"Comparative analysis of excretory\u2013secretory antigens of Trichinella spiralis and Trichinella britovi muscle larvae by two-dimensional difference gel electrophoresis and immunoblotting","volume":"10","author":"Bien","year":"2012","journal-title":"Proteome Sci."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0015","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0020","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/1743-422X-5-74","article-title":"Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance","volume":"5","author":"Branco","year":"2008","journal-title":"Virol. J."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0025","doi-asserted-by":"crossref","first-page":"S141","DOI":"10.1051\/parasite\/200108s2141","article-title":"The use of a synthetic antigen for the serological diagnosis of human trichinellosis","volume":"8","author":"Bruschi","year":"2001","journal-title":"Parasite"},{"key":"10.1016\/j.actatropica.2014.10.017_bib0030","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1136\/pmj.78.915.15","article-title":"New aspects of human trichinellosis: the impact of new Trichinella species","volume":"78","author":"Bruschi","year":"2002","journal-title":"Postgrad. Med. J."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0035","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.vetpar.2013.01.038","article-title":"Survey of Trichinella infections in domestic pigs from northern and eastern Henan, China","volume":"194","author":"Cui","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0040","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1007\/s00436-010-2206-2","article-title":"Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana","volume":"108","author":"Cui","year":"2011","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0045","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1016\/j.vaccine.2012.12.070","article-title":"Phage-displayed specific polypeptide antigens induce significant protective immunity against Trichinella spiralis infection in BALB\/c mice","volume":"31","author":"Cui","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.actatropica.2014.10.017_bib0050","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.pt.2005.12.006","article-title":"The re-emergence of trichinellosis in China?","volume":"22","author":"Cui","year":"2006","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0055","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2011.02.005","article-title":"The epidemiology of human trichinellosis in China during 2004\u20132009","volume":"118","author":"Cui","year":"2011","journal-title":"Acta Trop."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0060","doi-asserted-by":"crossref","first-page":"S120","DOI":"10.1051\/parasite\/200108s2120","article-title":"Possible presence of common tyvelose-containing glycans in Trichinella L1 larvae and embryonated eggs of several nematodes","volume":"8","author":"Dea-Ayuela","year":"2001","journal-title":"Parasite"},{"key":"10.1016\/j.actatropica.2014.10.017_bib0065","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.vetpar.2008.10.064","article-title":"Presidential address of ICT12 Conference: Trichinella and trichinellosis\u2014a never ending story","volume":"159","author":"Dupouy-Camet","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0070","series-title":"FAO\/WHO\/OIE Guidelines for the Surveillance, Management, Prevention and Control of Trichinellosis","first-page":"37","article-title":"Management and diagnosis of human trichinellosis","author":"Dupuy-Camet","year":"2007"},{"key":"10.1016\/j.actatropica.2014.10.017_bib0075","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1517\/14656566.3.8.1117","article-title":"Opinion on the diagnosis and treatment of human trichinellosis","volume":"3","author":"Dupouy-Camet","year":"2002","journal-title":"Exp. Opin. Pharmacother."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0080","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/S0304-4017(00)00354-X","article-title":"International Commission on Trichinellosis: recommendations on methods for the control of Trichinella in domestic and wild animals intended for human consumption","volume":"93","author":"Gamble","year":"2000","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0085","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1051\/parasite\/20041113","article-title":"International Commission on Trichinellosis: recommendations on the use of serological tests for the detection of Trichinella infection in animals and man","volume":"11","author":"Gamble","year":"2004","journal-title":"Parasite"},{"key":"10.1016\/j.actatropica.2014.10.017_bib0090","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1089\/fpd.2011.0985","article-title":"Comparison of artificial digestion and Baermann's methods for detection of Trichinella spiralis pre-encapsulated larvae in muscles with low-level infections","volume":"9","author":"Jiang","year":"2012","journal-title":"Foodborne Pathog. Dis."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0095","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1089\/fpd.2009.0445","article-title":"Sensitivity and optimization of artificial digestion in the inspection of meat for Trichinella spiralis","volume":"7","author":"Li","year":"2010","journal-title":"Foodborne Pathog. Dis."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0100","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1089\/fpd.2009.0472","article-title":"Early detection by polymerase chain reaction of migratory Trichinella spiralis larvae in blood of experimentally infected mice","volume":"7","author":"Li","year":"2010","journal-title":"Foodborne Pathog. Dis."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0105","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1016\/j.ijpara.2012.09.011","article-title":"Rapid detection of Trichinella spiralis larvae in muscles by loop-mediated isothermal amplification","volume":"42","author":"Li","year":"2012","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0110","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.exppara.2012.11.001","article-title":"Detection of circulating antigen in serum of mice infected with Trichinella spiralis by an IgY-IgM mAb sandwich ELISA","volume":"133","author":"Liu","year":"2013","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0115","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/ng.769","article-title":"The draft genome of the parasitic nematode Trichinella spiralis","volume":"43","author":"Mitreva","year":"2011","journal-title":"Nat. Genet."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0120","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.3201\/eid1712.110896","article-title":"Worldwide occurrence and impact of human trichinellosis, 1986\u20132009","volume":"17","author":"Murrell","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0125","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nmeth.1701","article-title":"SignalP 4.0: discriminating signal peptides from transmembrane regions","volume":"8","author":"Petersen","year":"2011","journal-title":"Nat. Methods"},{"key":"10.1016\/j.actatropica.2014.10.017_bib0130","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.ijpara.2006.08.007","article-title":"Comparative analysis of the excretory\u2013secretory proteome of the muscle larva of Trichinella pseudospiralis and Trichinella spiralis","volume":"37","author":"Robinson","year":"2007","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0135","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.sbi.2009.03.008","article-title":"The sequence-structure relationship and protein function prediction","volume":"19","author":"Sadowski","year":"2009","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0140","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.exppara.2013.03.004","article-title":"Cloning, expression and characterization of a Trichinella spiralis serine protease gene encoding a 35.5kDa protein","volume":"134","author":"Wang","year":"2013","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0145","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1756-3305-7-40","article-title":"Identification of early diagnostic antigens from major excretory\u2013secretory proteins of Trichinella spiralis muscle larvae using immunoproteomics","volume":"7","author":"Wang","year":"2014","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.actatropica.2014.10.017_bib0150","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.vetpar.2013.01.046","article-title":"Protein changes in Trichinella spiralis muscle larvae in vitro induced by bovine bile","volume":"194","author":"Wang","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0155","doi-asserted-by":"crossref","first-page":"139745","DOI":"10.1155\/2013\/139745","article-title":"Proteomic analysis of Trichinella spiralis muscle larval excretory\u2013secretory proteins recognized by early infection sera","volume":"2013","author":"Wang","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0160","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1007\/s00436-010-2102-9","article-title":"Protein change of intestinal epithelial cells induced in vitro by Trichinella spiralis infective larvae","volume":"108","author":"Wang","year":"2011","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0165","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0001-706X(98)00072-2","article-title":"Epidemiological, clinical and serological studies on trichinellosis in Henan Province, China","volume":"71","author":"Wang","year":"1998","journal-title":"Acta Trop."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0170","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1016\/j.jprot.2012.02.005","article-title":"Proteomic analysis of Trichinella spiralis proteins in intestinal epithelial cells after culture with their larvae by shotgun LC\u2013MS\/MS approach","volume":"75","author":"Wang","year":"2012","journal-title":"J. Proteomics."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0175","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1128\/CDLI.10.5.793-796.2003","article-title":"Development and evaluation of a Western blot kit for diagnosis of human trichinellosis","volume":"10","author":"Yera","year":"2003","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.actatropica.2014.10.017_bib0180","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1093\/bioinformatics\/17.9.847","article-title":"InterProScan\u2014an integration platform for the signature-recognition methods in InterPro","volume":"17","author":"Zdobnov","year":"2001","journal-title":"Bioinformatics"},{"key":"10.1016\/j.actatropica.2014.10.017_bib0185","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1186\/1756-3305-6-246","article-title":"Molecular characterization of Trichinella spiralis aminopeptidase and its potential as a novel vaccine candidate antigen against trichinellosis in BALB\/c mice","volume":"6","author":"Zhang","year":"2013","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.actatropica.2014.10.017_bib0190","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2010.04.014","article-title":"Serological proteome-oriented screening and application of antigens for the diagnosis of Schistosomiasis japonica","volume":"116","author":"Zhong","year":"2010","journal-title":"Acta Trop."}],"container-title":["Acta Tropica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X14003398?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X14003398?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T10:23:19Z","timestamp":1598437399000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0001706X14003398"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":38,"alternative-id":["S0001706X14003398"],"URL":"https:\/\/doi.org\/10.1016\/j.actatropica.2014.10.017","relation":{},"ISSN":["0001-706X"],"issn-type":[{"value":"0001-706X","type":"print"}],"subject":[],"published":{"date-parts":[[2015,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of a Trichinella spiralis 31kDa protein and its potential application for the serodiagnosis of trichinellosis","name":"articletitle","label":"Article Title"},{"value":"Acta Tropica","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actatropica.2014.10.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}