{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T03:46:29Z","timestamp":1789530389023,"version":"build-2803163510"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Tropica"],"published-print":{"date-parts":[[2016,6]]},"DOI":"10.1016\/j.actatropica.2016.02.024","type":"journal-article","created":{"date-parts":[[2016,3,2]],"date-time":"2016-03-02T08:01:11Z","timestamp":1456905671000},"page":"83-87","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"special_numbering":"C","title":["Identification of causative Leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP"],"prefix":"10.1016","volume":"158","author":[{"given":"Yu","family":"Koarashi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham G.","family":"C\u00e1ceres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencia Margarita Z\u00faniga","family":"Saca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa Elvira Palacios","family":"Flores","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adela Celis","family":"Trujillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luis Abanto","family":"Alvares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumiko","family":"Yoshimatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiro","family":"Arikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Katakura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihisa","family":"Hashiguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotomo","family":"Kato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.actatropica.2016.02.024_bib0005","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.actatropica.2006.07.001","article-title":"Clinical diagnosis of cutaneous leishmaniasis: a comparison study between standardized graded direct microscopy and ITS1-PCR of Giemsa-stained smears","volume":"99","author":"Al-Jawabreh","year":"2006","journal-title":"Acta Trop."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0010","doi-asserted-by":"crossref","first-page":"e35671","DOI":"10.1371\/journal.pone.0035671","article-title":"Leishmaniasis worldwide and global estimates of its incidence","volume":"7","author":"Alvar","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.actatropica.2016.02.024_bib0015","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/0035-9203(90)90025-A","article-title":"Biochemical characterization of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana by isoenzyme electrophoresis","volume":"84","author":"Arana","year":"1990","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0020","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1086\/518041","article-title":"Influence of Leishmania (Viannia) species on the response to antimonial treatment in patients with American tegumentary leishmaniasis","volume":"195","author":"Arevalo","year":"2007","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0025","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1017\/S0031182011001909","article-title":"In vitro and in vivo behaviour of sympatric Leishmania (V.) braziliensis: L. (V.) peruviana and their hybrids","volume":"139","author":"Cortes","year":"2012","journal-title":"Parasitology"},{"key":"10.1016\/j.actatropica.2016.02.024_bib0030","doi-asserted-by":"crossref","first-page":"296","DOI":"10.4269\/ajtmh.1994.50.296","article-title":"A general classification of New World Leishmania using numerical zymotaxonomy","volume":"50","author":"Cupolillo","year":"1994","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0035","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0001-706X(95)00094-U","article-title":"Putative Leishmania hybrids in the eastern andean valley of huanuco, Peru","volume":"59","author":"Dujardin","year":"1995","journal-title":"Acta Trop."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0040","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.diagmicrobio.2012.06.010","article-title":"Accurate and rapid species typing from cutaneous and mucocutaneous leishmaniasis lesions of the New World","volume":"74","author":"Fraga","year":"2012","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0045","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.diagmicrobio.2013.07.023","article-title":"HindII and SduI digests of heat-shock protein 70 PCR for Leishmania typing","volume":"77","author":"Fraga","year":"2013","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0050","doi-asserted-by":"crossref","first-page":"2294","DOI":"10.1128\/JCM.42.5.2294-2297.2004","article-title":"Culture-independent species typing of neotropical Leishmania for clinical validation of a PCR-based assay targeting heat shock protein 70 genes","volume":"42","author":"Garcia","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0055","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0060","doi-asserted-by":"crossref","first-page":"719","DOI":"10.4269\/ajtmh.2008.79.719","article-title":"Molecular mass screening to incriminate sand fly vectors of Andean-type cutaneous leishmaniasis in Ecuador and Peru","volume":"79","author":"Kato","year":"2008","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0065","doi-asserted-by":"crossref","first-page":"3661","DOI":"10.1128\/JCM.00498-10","article-title":"Use of FTA cards for direct sampling of patients lesions in the ecological study of cutaneous leishmaniasis","volume":"48","author":"Kato","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0070","doi-asserted-by":"crossref","first-page":"e0004336","DOI":"10.1371\/journal.pntd.0004336","article-title":"First evidence of a hybrid of Leishmania (Viannia) braziliensis\/L.: (V.) peruviana DNA detected from the phlebotomine sand fly Lutzomyia tejadai in Peru","volume":"10","author":"Kato","year":"2016","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0075","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1179\/2047773211Y.0000000014","article-title":"Diagnosis and identification of Leishmania spp. from Giemsa-stained slides, by real-time PCR and melting curve analysis in south-west of Iran","volume":"105","author":"Khademvatan","year":"2011","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0080","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.actatropica.2012.08.017","article-title":"RFLPs of ITS: ITS1 and hsp70 amplicons and sequencing of ITS1 of recent clinical isolates of Kala-azar from India and Bangladesh confirms the association of L. tropica with the disease","volume":"124","author":"Khanra","year":"2012","journal-title":"Acta Trop."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0085","article-title":"Evolution, classification and geographical distribution","volume":"vol. I","author":"Lainson","year":"1987"},{"key":"10.1016\/j.actatropica.2016.02.024_bib0090","doi-asserted-by":"crossref","first-page":"312","DOI":"10.4269\/ajtmh.1998.59.312","article-title":"Geographic distribution and clinical description of leishmaniasis cases in Peru","volume":"59","author":"Lucas","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0095","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1179\/000349802125000484","article-title":"DNA extraction and amplification of Leishmania from archived Giemsa-stained slides, for the diagnosis of cutaneous leishmaniasis by PCR","volume":"96","author":"Motazedian","year":"2001","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0100","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1186\/1756-3305-7-420","article-title":"Molecular diagnosis of cutaneous leishmaniasis and identification of the causative Leishmania species in Morocco by using three PCR-based assays","volume":"7","author":"Mouttaki","year":"2014","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.actatropica.2016.02.024_bib0105","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1128\/JCM.02029-06","article-title":"Molecular diagnosis of leishmaniasis: current status and future applications","volume":"45","author":"Reithinger","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0110","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1051\/parasite\/2012191081","article-title":"Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province Morocco","volume":"19","author":"Rhajaoui","year":"2012","journal-title":"Parasites"},{"key":"10.1016\/j.actatropica.2016.02.024_bib0115","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1051\/parasite\/1990653111","article-title":"Taxonomy of Leishmania: use of isoenzymes. Suggestions for a new classification","volume":"65","author":"Rioux","year":"1990","journal-title":"Ann. Parasitol. Hum. Comp."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0120","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1128\/JCM.44.2.459-467.2006","article-title":"Use of PCR-restriction fragment length polymorphism analysis to identify the main new world Leishmania species and analyze their taxonomic properties and polymorphism by application of the assay to clinical samples","volume":"44","author":"Rotureau","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0125","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.trstmh.2007.05.019","article-title":"Detection and species identification of Old World Leishmania in clinical samples using a PCR-based method","volume":"102","author":"Spanakos","year":"2008","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0130","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0001-706X(98)00076-X","article-title":"In vitro promastigote fitness of putative Leishmania (Viannia) braziliensis\/Leishmania (Viannia) peruviana hybrids","volume":"72","author":"Torrico","year":"1999","journal-title":"Acta Trop."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0135","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1097\/00001432-200304000-00006","article-title":"Diagnosis of cutaneous leishmaniasis","volume":"16","author":"Vega-L\u00f3pez","year":"2003","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0140","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1111\/j.1469-0691.2006.01422.x","article-title":"Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years","volume":"12","author":"Volpini","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.actatropica.2016.02.024_bib0145","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1086\/500326","article-title":"Development of a genetic assay to distinguish between Leishmania Viannia species on the basis of isoenzyme differences","volume":"42","author":"Zhang","year":"2006","journal-title":"Clin. Infect. Dis."}],"container-title":["Acta Tropica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X16300869?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X16300869?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T05:03:55Z","timestamp":1567659835000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0001706X16300869"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6]]},"references-count":29,"alternative-id":["S0001706X16300869"],"URL":"https:\/\/doi.org\/10.1016\/j.actatropica.2016.02.024","relation":{},"ISSN":["0001-706X"],"issn-type":[{"value":"0001-706X","type":"print"}],"subject":[],"published":{"date-parts":[[2016,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of causative Leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP","name":"articletitle","label":"Article Title"},{"value":"Acta Tropica","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actatropica.2016.02.024","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}