{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:38:06Z","timestamp":1787161086013,"version":"build-2736575974"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)"},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais (Fapemig)"},{"name":"Funda\u00e7\u00e3o de Apoio ao Ensino, Pesquisa e Extens\u00e3o (FEPE)"},{"name":"Programa Pesquisador Mineiro \u2212 PPM","award":["00923-15"],"award-info":[{"award-number":["00923-15"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Tropica"],"published-print":{"date-parts":[[2017,2]]},"DOI":"10.1016\/j.actatropica.2016.10.023","type":"journal-article","created":{"date-parts":[[2016,11,2]],"date-time":"2016-11-02T20:15:21Z","timestamp":1478117721000},"page":"106-113","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Molecular epidemiology of Brucella abortus isolated from cattle in Brazil, 2009\u20132013"],"prefix":"10.1016","volume":"166","author":[{"given":"Mayra Silva","family":"Oliveira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine Maria Seles","family":"Dorneles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Martins Filho","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Junior","given":"Ant\u00f4nio Augusto","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia","family":"Orzil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Gomes","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey Pereira","family":"Lage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.actatropica.2016.10.023_bib0005","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.mimet.2006.12.015","article-title":"Evaluation of Brucella MLVA typing for human brucellosis","volume":"69","author":"Al Dahouk","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0010","doi-asserted-by":"crossref","first-page":"e0136721","DOI":"10.1371\/journal.pone.0136721","article-title":"Molecular epidemiology of Brucella abortus in northern Ireland\u20141991 to 2012","volume":"10","author":"Allen","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0015","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.meegid.2008.10.007","article-title":"HOOF-print genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale","volume":"9","author":"Almendra","year":"2009","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0020","series-title":"Techniques for the Brucellosis Laboratory","author":"Alton","year":"1988"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0025","first-page":"271","article-title":"Detection of Brucella melitensis and Brucella abortus by DNA amplification","volume":"95","author":"Baily","year":"1992","journal-title":"J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0030","unstructured":"Brasil, 2001. Instru\u00e7\u00e3o Normativa Ministerial no 02\/2001. Instituir o Programa Nacional de Controle e Erradica\u00e7\u00e3o da Brucelose e da Tuberculose Animal. Minist\u00e9rio da Agricultura Pecu\u00e1ria e Abastecimento, Brasilia."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0035","unstructured":"Brasil, 2013. Projeto de amplia\u00e7\u00e3o da zona livre de febre aftosa com vacina\u00e7\u00e3o \u22122013. Anexo: Estudo soroepidemiol\u00f3gico, baseado em risco, para avaliar circula\u00e7\u00e3o viral na \u00e1rea proposta para amplia\u00e7\u00e3o da zona livre de febre aftosa. Relat\u00f3rio final. Minist\u00e9rio da Agricultura, Pecu\u00e1ria e Abastecimento, Bras\u00edlia, p. 38."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0040","first-page":"32","article-title":"Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR","author":"Bricker","year":"1994","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0045","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2180-3-15","article-title":"Brucella \u2018HOOF-Prints': strain typing by multi-locus analysis of variable number tandem repeats (VNTRs)","volume":"3","author":"Bricker","year":"2003","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0050","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1590\/S0102-09352009000700007","article-title":"Situa\u00e7\u00e3o epidemiol\u00f3gica da brucelose bovina no Estado do Mato Grosso do Sul","volume":"61","author":"Chate","year":"2009","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0055","series-title":"Brucellosis in Humans and Animals","author":"Corbel","year":"2006"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0060","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1186\/1471-2180-14-186","article-title":"Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16)","volume":"14","author":"Dorneles","year":"2014","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0065","series-title":"Guidelines for Coordinated Human and Animal Brucellosis Surveillance","author":"FAO","year":"2003"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0070","doi-asserted-by":"crossref","first-page":"e42514","DOI":"10.1371\/journal.pone.0042514","article-title":"MLVA16 typing of Portuguese human and animal Brucella melitensis and Brucella abortus isolates","volume":"7","author":"Ferreira","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0075","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.meegid.2013.06.021","article-title":"Investigating genetic diversity of Brucella abortus and Brucella melitensis in Italy with MLVA-16","volume":"19","author":"Garofolo","year":"2013","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0080","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1186\/1471-2180-9-230","article-title":"Application and evaluation of the MLVA typing assay for the Brucella abortus strains isolated in Korea","volume":"9","author":"Her","year":"2009","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0085","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1128\/JCM.26.11.2465-2466.1988","article-title":"Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity","volume":"26","author":"Hunter","year":"1988","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0090","unstructured":"IBGE, 2014. Pesquisa Pecu\u00e1ria Municipal (PPM). Instituto Brasileiro de Geografia e Estat\u00edstica, Brasil."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0095","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/j.trstmh.2010.08.002","article-title":"MLVA typing and antibiotic susceptibility of Brucella human isolates from Liaonin, Chinag","volume":"104","author":"Jiang","year":"2010","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0100","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1471-2180-6-9","article-title":"Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay","volume":"6","author":"Le Fleche","year":"2006","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0105","doi-asserted-by":"crossref","first-page":"e115319","DOI":"10.1371\/journal.pone.0115319","article-title":"Human brucellosis in Maghreb: existence of a lineage related to socio-historical connections with Europe","volume":"9","author":"Lounes","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0110","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1017\/S0950268807008795","article-title":"Brucella isolated in humans and animals in Latin America from 1968 to 2006","volume":"136","author":"Lucero","year":"2008","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0115","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.3201\/eid1809.120069","article-title":"Brucellosis in takins, China","volume":"18","author":"Luo","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0120","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.jhin.2008.01.003","article-title":"Use of MLVA-16 typing to trace the source of a laboratory-acquired Brucella infection","volume":"68","author":"Marianelli","year":"2008","journal-title":"J. Hosp. Infect."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0125","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0378-1135(02)00249-3","article-title":"Brucellosis in sub-Saharan Africa: epidemiology control and impact","volume":"90","author":"McDermott","year":"2002","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0130","doi-asserted-by":"crossref","first-page":"e94168","DOI":"10.1371\/journal.pone.0094168","article-title":"Brucella melitensis in France: persistence in wildlife and probable spillover from Alpine ibex to domestic animals","volume":"9","author":"Mick","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0135","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0081152","article-title":"Biotyping and genotyping (MLVA16) of Brucella abortus isolated from cattle in Brazil, 1977 to 2008","volume":"8","author":"Minharro","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0140","first-page":"117","article-title":"Ocorr\u00eancia de brucelose bovina no Estado do Par\u00e1 confirmada por m\u00e9todos sorol\u00f3gicos","volume":"22","author":"Moln\u00e1r","year":"2000","journal-title":"Rev. Bras. Med. Vet."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0145","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1017\/S0950268815002575","article-title":"Systematic review of brucellosis in the Middle East: disease frequency in ruminants and humans and risk factors for human infection","volume":"144","author":"Musallam","year":"2016","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0150","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1590\/S0102-09352009000700008","article-title":"Situa\u00e7\u00e3o epidemiol\u00f3gica da brucelose bovina no Estado de Mato Grosso","volume":"61","author":"Negreiros","year":"2009","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0155","series-title":"Bovine Brucellosis, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals","author":"OIE","year":"2009"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0160","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1590\/S0102-09352009000700016","article-title":"Situa\u00e7\u00e3o epidemiol\u00f3gica da brucelose bovina no Estado do Tocantins","volume":"61","author":"Ogata","year":"2009","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"issue":"5","key":"10.1016\/j.actatropica.2016.10.023_bib0165","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1590\/0103-8478cr20150065","article-title":"Validation of the multiplex PCR for identification of Brucella spp","volume":"46","author":"Orzil","year":"2016","journal-title":"Ci\u00eancia Rural"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0170","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/j.1472-765X.1989.tb00262.x","article-title":"Rapid extraction of bacterial genomic DNA with guanidium thiocyanate","volume":"8","author":"Pitcher","year":"1989","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0175","doi-asserted-by":"crossref","first-page":"01","DOI":"10.1590\/S0102-09352009000700001","article-title":"Estudos de preval\u00eancia da brucelose bovina no \u00e2mbito do Programa Nacional de Controle e Erradica\u00e7\u00e3o de Brucelose e Tuberculose","volume":"61","author":"Poester","year":"2009","journal-title":"Introdu\u00e7\u00e3o. Arq. Bras. Med. Vet. Zootec."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0180","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"2001"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0185","doi-asserted-by":"crossref","first-page":"149","DOI":"10.20506\/rst.32.1.2189","article-title":"Molecular characterisation of Brucella species","volume":"32","author":"Scholz","year":"2013","journal-title":"Rev. Sci. Tech."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0190","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1590\/S0102-09352009000700013","article-title":"Situa\u00e7\u00e3o epidemiol\u00f3gica da brucelose bovina no Estado de Santa Catarina","volume":"61","author":"Sikusawa","year":"2009","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0195","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1590\/S0074-02761998000500004","article-title":"Molecular epidemiologic typing systems of bacterial pathogens: current issues and perspectives","volume":"93","author":"Struelens","year":"1998","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.actatropica.2016.10.023_bib0200","doi-asserted-by":"crossref","first-page":"2734","DOI":"10.1128\/JCM.00533-10","article-title":"Epidemiological and phylogenetic analysis of Spanish human Brucella melitensis strains by multiple-locus variable-number tandem-repeat typing, hypervariable octameric oligonucleotide fingerprinting, and rpoB typing","volume":"48","author":"Valdezate","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0205","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1101\/gr.10.7.899","article-title":"Minisatellites: mutability and genome architecture","volume":"10","author":"Vergnaud","year":"2000","journal-title":"Genome Res."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0210","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.1128\/JCM.02039-05","article-title":"Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp","volume":"44","author":"Whatmore","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0215","doi-asserted-by":"crossref","first-page":"4120","DOI":"10.1099\/ijs.0.065482-0","article-title":"Brucella papionis sp. nov., isolated from baboons (Papio spp.)","volume":"64","author":"Whatmore","year":"2014","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"10.1016\/j.actatropica.2016.10.023_bib0220","doi-asserted-by":"crossref","first-page":"e0115932","DOI":"10.1371\/journal.pone.0115932","article-title":"Genotyping of human Brucella melitensis biovar 3 isolated from Shanxi Province in China by MLVA16 and HOOF","volume":"10","author":"Xiao","year":"2015","journal-title":"PLoS One"}],"container-title":["Acta Tropica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X1630599X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X1630599X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T23:06:16Z","timestamp":1601161576000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0001706X1630599X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":44,"alternative-id":["S0001706X1630599X"],"URL":"https:\/\/doi.org\/10.1016\/j.actatropica.2016.10.023","relation":{},"ISSN":["0001-706X"],"issn-type":[{"value":"0001-706X","type":"print"}],"subject":[],"published":{"date-parts":[[2017,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular epidemiology of Brucella abortus isolated from cattle in Brazil, 2009\u20132013","name":"articletitle","label":"Article Title"},{"value":"Acta Tropica","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actatropica.2016.10.023","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}