{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,8]],"date-time":"2026-11-08T11:44:24Z","timestamp":1794138264576,"version":"4.8.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Tropica"],"published-print":{"date-parts":[[2017,5]]},"DOI":"10.1016\/j.actatropica.2017.01.008","type":"journal-article","created":{"date-parts":[[2017,1,17]],"date-time":"2017-01-17T18:03:37Z","timestamp":1484676217000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":43,"special_numbering":"C","title":["Application of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samples"],"prefix":"10.1016","volume":"169","author":[{"given":"Hiroki","family":"Hashizume","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megumi","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello Otake","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumire","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tippayarat","family":"Yoonuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surapol","family":"Sanguankiat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiengkham","family":"Pongvongsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Moji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshifumi","family":"Minamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.actatropica.2017.01.008_bib0005","series-title":"Molecular Biology of the Cell","author":"Alberts","year":"2002"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0010","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.pt.2008.08.011","article-title":"Opisthorchis viverrini: an underestimated parasite in world health","volume":"24","author":"Andrews","year":"2008","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0015","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.pt.2015.06.013","article-title":"Diverse applications of environmental DNA methods in parasitology","volume":"31","author":"Bass","year":"2015","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0020","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.vetpar.2011.07.013","article-title":"A review of parasitic zoonoses in a changing Southeast Asia","volume":"182","author":"Conlan","year":"2011","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0025","doi-asserted-by":"crossref","first-page":"e23398","DOI":"10.1371\/journal.pone.0023398","article-title":"Persistence of environmental DNA in freshwater ecosystems","volume":"6","author":"Dejean","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0030","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1098\/rsbl.2008.0118","article-title":"Species detection using environmental DNA from water samples","volume":"4","author":"Ficetola","year":"2008","journal-title":"Biol. Lett."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0035","doi-asserted-by":"crossref","first-page":"e41781","DOI":"10.1371\/journal.pone.0041781","article-title":"Investigating the potential use of environmental DNA (eDNA) for genetic monitoring of marine mammals","volume":"7","author":"Foote","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0040","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1111\/1365-2664.12392","article-title":"A basin-scale application of environmental DNA assessment for rare endemic species and closely related exotic species in rivers: a case study of giant salamanders in Japan","volume":"52","author":"Fukumoto","year":"2015","journal-title":"J. Appl. Ecol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0045","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1899\/13-046.1","article-title":"Environmental DNA as a new method for early detection of New Zealand mudsnails (Potamopyrgus antipodarum)","volume":"32","author":"Goldberg","year":"2013","journal-title":"Freshw. Sci."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0050","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.parint.2011.06.015","article-title":"Raw attitudes, wetland cultures, life-cycles: socio-cultural dynamics relating to Opisthorchis viverrini in the Mekong Basin","volume":"61","author":"Grundy-Warr","year":"2012","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0055","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1111\/1755-0998.12461","article-title":"Evaluating environmental DNA-based quantification of ranavirus infection in wood frog populations","volume":"16","author":"Hall","year":"2015","journal-title":"Mol. Ecol. Resour."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0060","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1890\/14-1530.1","article-title":"Development and application of an eDNA method to detect and quantify a pathogenic parasite in aquatic ecosystems","volume":"25","author":"Huver","year":"2015","journal-title":"Ecol. Appl."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0065","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3354\/dao02423","article-title":"Evaluation of a filtration-based method for detecting Batrachochytrium dendrobatidis in natural bodies of water","volume":"97","author":"Hyman","year":"2012","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0070","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1007\/s00436-008-1026-0","article-title":"Detection of Opisthorchis viverrini in infected bithynid snails by real-time fluorescence resonance energy transfer PCR-based method and melting curve analysis","volume":"103","author":"Intapan","year":"2008","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0075","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.actatropica.2003.05.001","article-title":"Taxonomy and biology of liver flukes","volume":"88","author":"Kaewkes","year":"2003","journal-title":"Acta Trop."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0080","doi-asserted-by":"crossref","first-page":"666","DOI":"10.3748\/wjg.14.666","article-title":"Opisthorchis viverrini: the carcinogenic human liver fluke","volume":"14","author":"Kaewpitoon","year":"2008","journal-title":"World J. Gastroenterol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0085","doi-asserted-by":"crossref","first-page":"464","DOI":"10.4269\/ajtmh.2012.11-0217","article-title":"Exceptionally high prevalence of infection of Bithynia siamensis goniomphalos with Opisthorchis viverrini cercariae in different wetlands in Thailand and Lao PDR","volume":"86","author":"Kiatsopit","year":"2012","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0090","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1128\/JCM.02011-08","article-title":"PCR diagnosis of Opisthorchis viverrini and Haplorchis taichui infections in a Lao community in an area of endemicity and comparison of diagnostic methods for parasitological field surveys","volume":"47","author":"Lovis","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0095","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.vetmic.2008.09.081","article-title":"Detection of cyprinid herpesvirus 3 DNA in river water during and after an outbreak","volume":"135","author":"Minamoto","year":"2009","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0100","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s10201-011-0362-4","article-title":"Surveillance of fish species composition using environmental DNA","volume":"13","author":"Minamoto","year":"2012","journal-title":"Limnology"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0105","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s10201-014-0440-5","article-title":"Monitoring of fish pathogenic viruses in natural lakes in Yunnan, China","volume":"16","author":"Minamoto","year":"2015","journal-title":"Limnology"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0110","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s10201-015-0457-4","article-title":"Techniques for the practical collection of environmental DNA: filter selection, preservation, and extraction","volume":"17","author":"Minamoto","year":"2016","journal-title":"Limnology"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0115","doi-asserted-by":"crossref","first-page":"150088","DOI":"10.1098\/rsos.150088","article-title":"Mifish, a set of universal PCR primers for metabarcording environmental DNA from fishes: detection of more than 230 subtropical marine species","volume":"2","author":"Miya","year":"2015","journal-title":"R. Soc. Open Sci."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0120","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1017\/S0022149X13000709","article-title":"Is Opisthorchis viverrini an avian liver fluke?","volume":"89","author":"Nawa","year":"2015","journal-title":"J. Helminthol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0125","first-page":"189","article-title":"Emergence of Opisthorchis viverrini cercariae from naturally infected Bithynia (Digoniostoma) siamensis goniomphalos","volume":"36","author":"Phongsasakulchoti","year":"2005","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0130","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/2046-9063-8-16","article-title":"Temporal distribution of genetically homogenous \u2018free-living\u2019 Hematodinium sp. in a Delmarva coastal ecosystem","volume":"8","author":"Pitula","year":"2012","journal-title":"Aquat. Biosys."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0135","series-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2014"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0140","doi-asserted-by":"crossref","first-page":"4023","DOI":"10.1002\/ece3.1272","article-title":"The application of eDNA for monitoring of the Great Crested Newt in the UK","volume":"4","author":"Rees","year":"2014","journal-title":"Ecol. Evol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0145","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1111\/1365-2664.12306","article-title":"The detection of aquatic animal species using environmental DNA \u2013 a review of eDNA as a survey tool in ecology","volume":"51","author":"Rees","year":"2014","journal-title":"J. Appl. Ecol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0150","doi-asserted-by":"crossref","first-page":"253","DOI":"10.3347\/kjp.2008.46.4.253","article-title":"Fishborne trematode metacercariae detected in freshwater fish from Vientiane municipality and Savannakhet province, Lao PDR","volume":"46","author":"Rim","year":"2008","journal-title":"Korean J. Parasitol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0155","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1111\/1755-0998.12505","article-title":"Fine-tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems","volume":"16","author":"Robson","year":"2016","journal-title":"Mol. Ecol. Resour."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0160","doi-asserted-by":"crossref","first-page":"857","DOI":"10.7314\/APJCP.2016.17.2.857","article-title":"Reinfection by Opisthorchis viverrini after treatment with Praziquantel","volume":"17","author":"Saengsawang","year":"2016","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0165","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s00436-011-2804-7","article-title":"Rapid detection and differentiation of Clonorchis sinensis and Opisthorchis viverrini eggs in human fecal samples using a duplex real-time fluorescence resonance energy transfer PCR and melting curve analysis","volume":"111","author":"Sanpool","year":"2012","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0170","first-page":"28","article-title":"Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of Lao PDR","volume":"41","author":"Sato","year":"2010","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0175","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1017\/S0022149X14000261","article-title":"Patterns of trematode infections of Opisthorchis viverrini (Opisthorchiidae) and Haplorchis taichui (Heterophyidae) in human populations from two villages in Savannakhet Province, Lao PDR","volume":"89","author":"Sato","year":"2015","journal-title":"J. Helminthol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0180","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1371\/journal.pmed.0040201","article-title":"Liver fluke induces cholangiocarcinoma","volume":"4","author":"Sripa","year":"2007","journal-title":"PLoS Med."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0185","doi-asserted-by":"crossref","first-page":"e56584","DOI":"10.1371\/journal.pone.0056584","article-title":"Using environmental DNA to estimate the distribution of an invasive fish species in ponds","volume":"8","author":"Takahara","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0190","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1645\/GE-2764.1","article-title":"Discovery of Opisthorchis lobatus (Trematoda: Opisthorchiidae): a new record of small liver flukes in the Greater Mekong sub-region","volume":"97","author":"Thaenkham","year":"2011","journal-title":"J. Parasitol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0195","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1111\/j.1365-294X.2011.05418.x","article-title":"Monitoring endangered freshwater biodiversity using environmental DNA","volume":"21","author":"Thomsen","year":"2012","journal-title":"Mol. Ecol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0200","article-title":"Risk factors for Opisthorchis viverrini infection among schoolchildren in Lao PDR","volume":"43","author":"Tomokawa","year":"2012","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0205","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1111\/2041-210X.12206","article-title":"Particle size distribution and optimal capture of aqueous macrobial eDNA","volume":"5","author":"Turner","year":"2014","journal-title":"Methods Ecol. Evol."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0210","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.foodres.2014.06.051","article-title":"Climate change and foodborne transmission of parasites: a consideration of possible interactions and impacts for selected parasites","volume":"68","author":"Utaaker","year":"2015","journal-title":"Food Res. Int."},{"key":"10.1016\/j.actatropica.2017.01.008_bib0215","first-page":"324","article-title":"Opisthorchis viverrini metacercaria in Thai freshwater fish","volume":"29","author":"Waikagul","year":"1998","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0220","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1017\/S0031182001007235","article-title":"Development of a PCR-based method for the detection of Opisthorchis viverrini in experimentally infected hamsters","volume":"122","author":"Wongratanacheewin","year":"2001","journal-title":"Parasitology"},{"key":"10.1016\/j.actatropica.2017.01.008_bib0225","doi-asserted-by":"crossref","first-page":"2192","DOI":"10.1128\/AEM.01561-10","article-title":"Field detection of Schistosoma japonicum cercariae in environmental water samples by quantitative PCR","volume":"77","author":"Worrell","year":"2011","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Acta Tropica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X16305563?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X16305563?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,5]],"date-time":"2018-09-05T02:21:46Z","timestamp":1536114106000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0001706X16305563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":45,"alternative-id":["S0001706X16305563"],"URL":"https:\/\/doi.org\/10.1016\/j.actatropica.2017.01.008","relation":{},"ISSN":["0001-706X"],"issn-type":[{"value":"0001-706X","type":"print"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Application of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samples","name":"articletitle","label":"Article Title"},{"value":"Acta Tropica","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actatropica.2017.01.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}