{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T09:22:27Z","timestamp":1762075347897,"version":"build-2065373602"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2018,6,28]],"date-time":"2018-06-28T00:00:00Z","timestamp":1530144000000},"content-version":"am","delay-in-days":270,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["87397"],"award-info":[{"award-number":["87397"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["IZLSZ3 149015"],"award-info":[{"award-number":["IZLSZ3 149015"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Tropica"],"published-print":{"date-parts":[[2017,10]]},"DOI":"10.1016\/j.actatropica.2017.06.012","type":"journal-article","created":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T00:45:53Z","timestamp":1497919553000},"page":"49-55","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["PCR-based verification of positive rapid diagnostic tests for intestinal protozoa infections with variable test band intensity"],"prefix":"10.1016","volume":"174","author":[{"given":"S\u00f6ren L.","family":"Becker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascal","family":"Mertens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathias","family":"Herrmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leyli","family":"Zondie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsey","family":"Beyleveld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Gerber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"du Randt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe","family":"P\u00fchse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl","family":"Walter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrg","family":"Utzinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.actatropica.2017.06.012_bib0005","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1007\/s10096-016-2722-9","article-title":"Molecular detection of common intestinal parasites: a performance evaluation of the BD Max Enteric Parasite Panel","volume":"35","author":"Batra","year":"2016","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0010","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1471-2334-13-37","article-title":"Persistent digestive disorders in the tropics: causative infectious pathogens and reference diagnostic tests","volume":"13","author":"Becker","year":"2013","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0015","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1055\/s-0034-1384526","article-title":"Intestinal cryptosporidiosis in a 6-year-old child with alpha1-antitrypsin deficiency","volume":"226","author":"Becker","year":"2014","journal-title":"Klin. Padiatr."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0020","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.cmi.2015.02.016","article-title":"Combined stool-based multiplex PCR and microscopy for enhanced pathogen detection in patients with persistent diarrhoea and asymptomatic controls from C\u00f4te d'Ivoire","volume":"21","author":"Becker","year":"2015","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0025","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES2015.20.23.21151","article-title":"Application in Europe of a urine-based rapid diagnostic test for confirmation of Schistosoma mansoni infection in migrants from endemic areas","volume":"20","author":"Becker","year":"2015","journal-title":"Euro Surveill."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0030","doi-asserted-by":"crossref","first-page":"e0004818","DOI":"10.1371\/journal.pntd.0004818","article-title":"Experiences and lessons from a multicountry NIDIAG study on persistent digestive disorders in the tropics","volume":"10","author":"Becker","year":"2016","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0035","doi-asserted-by":"crossref","first-page":"e0004853","DOI":"10.1371\/journal.pntd.0004853","article-title":"Clinical research on neglected tropical diseases: challenges and solutions","volume":"10","author":"Boelaert","year":"2016","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0040","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1007\/s12639-015-0645-5","article-title":"Low sensitivity of the ImmunocardSTAT Crypto\/Giardia rapid assay test for the detection of Giardia and Cryptosporidium in fecal samples from children living in Libreville, Central Africa","volume":"40","author":"Bouyou-Akotet","year":"2016","journal-title":"J. Parasit. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0045","doi-asserted-by":"crossref","first-page":"e2109","DOI":"10.1371\/journal.pntd.0002109","article-title":"Accuracy of urine circulating cathodic antigen test for the diagnosis of Schistosoma mansoni in preschool-aged children before and after treatment","volume":"7","author":"Coulibaly","year":"2013","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0050","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1093\/cid\/ciw391","article-title":"A prospective longitudinal cohort to investigate the effects of early life giardiasis on growth and all cause diarrhea","volume":"63","author":"Donowitz","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0055","doi-asserted-by":"crossref","first-page":"2712","DOI":"10.1001\/jama.2016.7833","article-title":"Persistent diarrhea: a clinical review","volume":"315","author":"DuPont","year":"2016","journal-title":"JAMA"},{"key":"10.1016\/j.actatropica.2017.06.012_bib0060","doi-asserted-by":"crossref","first-page":"e0005573","DOI":"10.1371\/journal.pntd.0005573","article-title":"Associations between selective attention and soil-transmitted helminth infections, socioeconomic status, and physical fitness in disadvantaged children in Port Elizabeth, South Africa: an observational study","volume":"11","author":"Gall","year":"2017","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0065","doi-asserted-by":"crossref","first-page":"e2865","DOI":"10.1371\/journal.pntd.0002865","article-title":"The global burden of disease study 2010: interpretation and implications for the neglected tropical diseases","volume":"8","author":"Hotez","year":"2014","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0070","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.gtc.2006.03.006","article-title":"An updated review on Cryptosporidium and Giardia","volume":"35","author":"Huang","year":"2006","journal-title":"Gastroenterol. Clin. North Am."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0075","doi-asserted-by":"crossref","first-page":"e1677","DOI":"10.1371\/journal.pntd.0001677","article-title":"High prevalence of Giardia duodenalis assemblage B infection and association with underweight in Rwandan children","volume":"6","author":"Ignatius","year":"2012","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0080","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1128\/JCM.41.2.623-626.2003","article-title":"Evaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens","volume":"41","author":"Johnston","year":"2003","journal-title":"J. Clin. Microbiol."},{"issue":"19","key":"10.1016\/j.actatropica.2017.06.012_bib0085","first-page":"e191","volume":"22","author":"Laude","year":"2016","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0090","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1128\/JCM.02100-15","article-title":"Evaluation of the BD Max Enteric Parasite Panel for clinical diagnostics","volume":"54","author":"M\u00f6lling","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0095","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1186\/s13071-016-1761-5","article-title":"Intestinal parasites, growth and physical fitness of schoolchildren in poor neighbourhoods of Port Elizabeth, South Africa: a cross-sectional survey","volume":"9","author":"M\u00fcller","year":"2016","journal-title":"Parasit Vectors"},{"key":"10.1016\/j.actatropica.2017.06.012_bib0100","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.1128\/JCM.00765-16","article-title":"Multi-center evaluation of the BD MAX Enteric Parasite RT-PCR assay for the detection of Giardia duodenalis, Cryptosporidium hominis and C. parvum and Entamoeba histolytica","volume":"54","author":"Madison-Antenucci","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0105","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1128\/JCM.02877-13","article-title":"Detection of intestinal protozoa in the clinical laboratory","volume":"52","author":"McHardy","year":"2014","journal-title":"J. Clin. Microbiol."},{"issue":"Suppl. 4","key":"10.1016\/j.actatropica.2017.06.012_bib0110","doi-asserted-by":"crossref","first-page":"S271","DOI":"10.1093\/cid\/cis762","article-title":"A systematic review and meta-analysis of the association between Giardia lamblia and endemic pediatric diarrhea in developing countries","volume":"55","author":"Muhsen","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0115","doi-asserted-by":"crossref","first-page":"2197","DOI":"10.1016\/S0140-6736(12)61689-4","article-title":"Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990\u20132010: a systematic analysis for the Global Burden of Disease study 2010","volume":"380","author":"Murray","year":"2012","journal-title":"Lancet"},{"key":"10.1016\/j.actatropica.2017.06.012_bib0120","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s10096-011-1332-9","article-title":"Evaluation of an immunochromatographic assay: Giardia-Strip (Coris BioConcept) for detection of Giardia intestinalis in human fecal specimens","volume":"31","author":"Nguyen","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0125","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1017\/S0950268815002514","article-title":"Genotyping of Giardia duodenalis isolates in asymptomatic children attending daycare centre: evidence of high risk for anthroponotic transmission","volume":"144","author":"Oliveira-Arbex","year":"2016","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0130","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1007\/s10096-006-0088-0","article-title":"Evaluation of the immunochromatographic CORIS Giardia-Strip test for rapid diagnosis of Giardia lamblia","volume":"25","author":"Oster","year":"2006","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0135","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.trstmh.2012.05.011","article-title":"Epidemiological, parasitological and molecular aspects of Giardia duodenalis infection in children attending public daycare centers in southeastern Brazil","volume":"106","author":"Santos","year":"2012","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0140","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1097\/QCO.0b013e328357e569","article-title":"Burden of disease from cryptosporidiosis","volume":"25","author":"Shirley","year":"2012","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0145","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.mimet.2016.08.017","article-title":"Giardiasis: an update review on sensitivity and specificity of methods for laboratorial diagnosis","volume":"129","author":"Soares","year":"2016","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.actatropica.2017.06.012_bib0150","doi-asserted-by":"crossref","first-page":"e0004729","DOI":"10.1371\/journal.pntd.0004729","article-title":"The burden of Cryptosporidium diarrheal disease among children <24months of age in moderate\/high mortality regions of sub-Saharan Africa and South Asia, utilizing data from the Global Enteric Multicenter Study (GEMS)","volume":"10","author":"Sow","year":"2016","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0155","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S1473-3099(15)00349-7","article-title":"Effect of sanitation and water treatment on intestinal protozoa infection: a systematic review and meta-analysis","volume":"16","author":"Speich","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0160","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1186\/s13071-017-2111-y","article-title":"Cryptosporidium and Giardia in Africa: current and future challenges","volume":"10","author":"Squire","year":"2017","journal-title":"Parasit Vectors"},{"key":"10.1016\/j.actatropica.2017.06.012_bib0165","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1128\/CMR.00017-09","article-title":"Clinical significance of enteric protozoa in the immunosuppressed human population","volume":"22","author":"Stark","year":"2009","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0170","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1097\/MOG.0000000000000135","article-title":"The intestinal protozoa: emerging impact on global health and development","volume":"31","author":"Turkeltaub","year":"2015","journal-title":"Curr. Opin. Gastroenterol."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0175","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1017\/S0031182014000419","article-title":"Application of PCR-based methods for diagnosis of intestinal parasitic infections in the clinical laboratory","volume":"141","author":"Verweij","year":"2014","journal-title":"Parasitology"},{"key":"10.1016\/j.actatropica.2017.06.012_bib0180","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1111\/j.1469-0691.2006.01457.x","article-title":"Evaluation of seven commercial antigen detection tests for Giardia and Cryptosporidium in stool samples","volume":"12","author":"Weitzel","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0185","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1186\/s12889-015-2636-y","article-title":"Disease, activity and schoolchildren's health (DASH) in Port Elizabeth, South Africa : a study protocol","volume":"15","author":"Yap","year":"2015","journal-title":"BMC Public Health"},{"key":"10.1016\/j.actatropica.2017.06.012_bib0190","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.cmi.2015.03.015","article-title":"Clinical consequences of new diagnostic tools for intestinal parasites","volume":"21","author":"van Lieshout","year":"2015","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.actatropica.2017.06.012_bib0195","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.mimet.2015.01.016","article-title":"Comparison of four rapid diagnostic tests, ELISA, microscopy and PCR for the detection of Giardia lamblia, Cryptosporidium spp. and Entamoeba histolytica in feces","volume":"110","author":"van den Bossche","year":"2015","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.actatropica.2017.06.012_bib0200","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/1475-2875-8-90","article-title":"Test characteristics of two rapid antigen detection tests (SD FK50 and SD FK60) for the diagnosis of malaria in returned travellers","volume":"8","author":"van der Palen","year":"2009","journal-title":"Malar. J."}],"container-title":["Acta Tropica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X17304151?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X17304151?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T09:18:41Z","timestamp":1762075121000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0001706X17304151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10]]},"references-count":40,"alternative-id":["S0001706X17304151"],"URL":"https:\/\/doi.org\/10.1016\/j.actatropica.2017.06.012","relation":{},"ISSN":["0001-706X"],"issn-type":[{"type":"print","value":"0001-706X"}],"subject":[],"published":{"date-parts":[[2017,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"PCR-based verification of positive rapid diagnostic tests for intestinal protozoa infections with variable test band intensity","name":"articletitle","label":"Article Title"},{"value":"Acta Tropica","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actatropica.2017.06.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}