{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:23:16Z","timestamp":1792513396013,"version":"4.5.0"},"reference-count":98,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100011893","name":"Pan American Health Organization","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100011893","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100014054","name":"Drugs for Neglected Disease Initiative","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100014054","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Tropica"],"published-print":{"date-parts":[[2018,8]]},"DOI":"10.1016\/j.actatropica.2017.09.020","type":"journal-article","created":{"date-parts":[[2017,9,29]],"date-time":"2017-09-29T19:01:09Z","timestamp":1506711669000},"page":"29-37","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":49,"special_numbering":"C","title":["The applicability of real-time PCR in the diagnostic of cutaneous leishmaniasis and parasite quantification for clinical management: Current status and perspectives"],"prefix":"10.1016","volume":"184","author":[{"given":"Otacilio C.","family":"Moreira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaida E.","family":"Yadon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Cupolillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"14","key":"10.1016\/j.actatropica.2017.09.020_bib0005","doi-asserted-by":"crossref","first-page":"1891","DOI":"10.1017\/S0031182014001280","article-title":"Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR","volume":"141","author":"Adams","year":"2014","journal-title":"Parasitology"},{"key":"10.1016\/j.actatropica.2017.09.020_bib0010","article-title":"Leishmania infections: molecular targets and diagnosis","volume":"2017","author":"Akhoundi","year":"2017","journal-title":"Mol. Aspects Med."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0015","doi-asserted-by":"crossref","first-page":"e35671","DOI":"10.1371\/journal.pone.0035671","article-title":"WHO leishmaniasis control team. leishmaniasis worldwide and global estimates of its incidence","volume":"7","author":"Alvar","year":"2012","journal-title":"PLoS One"},{"issue":"8","key":"10.1016\/j.actatropica.2017.09.020_bib0020","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1099\/jmm.0.05193-0","article-title":"Detection and genotyping by real-time PCR\/RFLP analyses of Giardia duodenalis from human faeces","volume":"52","author":"Amar","year":"2003","journal-title":"J. Med. Microbiol."},{"issue":"10","key":"10.1016\/j.actatropica.2017.09.020_bib0025","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1590\/0074-02760160138","article-title":"Asymptomatic infection with American cutaneous leishmaniasis: epidemiological and immunological studies","volume":"111","author":"Andrade-Narvaez","year":"2016","journal-title":"Mem. Inst. Oswaldo Cruz"},{"issue":"12","key":"10.1016\/j.actatropica.2017.09.020_bib0030","doi-asserted-by":"crossref","first-page":"e202","DOI":"10.1093\/cid\/ciw670","article-title":"Diagnosis and treatment of leishmaniasis: clinical practice guidelines by the infectious diseases society of america (IDSA) and the american society of tropical medicine and hygiene (ASTMH)","volume":"63","author":"Aronson","year":"2016","journal-title":"Clin. Infect. Dis."},{"issue":"2","key":"10.1016\/j.actatropica.2017.09.020_bib0035","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1743-422X-2-61","article-title":"Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey","volume":"11","author":"Aryee","year":"2005","journal-title":"Virol. J."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0040","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1590\/S0074-02761983000100002","article-title":"Diagn\u00f3stico histopatol\u00f3gico de la leishmaniasis cut\u00e1nea y cut\u00e1neo-mucosa en Bolivia","volume":"78","author":"Azogue","year":"1983","journal-title":"Mem. Inst. Oswaldo Cruz"},{"issue":"6","key":"10.1016\/j.actatropica.2017.09.020_bib0045","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1128\/JCM.02258-07","article-title":"Quantitative real-time PCR is not more sensitive than conventional PCR","volume":"46","author":"Bastien","year":"2008","journal-title":"J. Clin. Microbiol."},{"issue":"4","key":"10.1016\/j.actatropica.2017.09.020_bib0050","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1128\/JCM.44.4.1435-1439.2006","article-title":"Comparison of PCR assays for diagnosis of cutaneous leishmaniasis","volume":"44","author":"Bensoussan","year":"2006","journal-title":"J. Clin. Microbiol."},{"issue":"10","key":"10.1016\/j.actatropica.2017.09.020_bib0055","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1111\/j.1365-3156.2009.02361.x","article-title":"Species diversity of Leishmania (Viannia) parasites circulating in an endemic area for cutaneous leishmaniasis located in the Atlantic rainforest region of northeastern Brazil","volume":"14","author":"Brito","year":"2009","journal-title":"Trop. Med. Int. Health"},{"issue":"3","key":"10.1016\/j.actatropica.2017.09.020_bib0060","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1128\/CMR.00074-09","article-title":"Validation of laboratory-developed molecular assays for infectious diseases","volume":"23","author":"Burd","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"issue":"3","key":"10.1016\/j.actatropica.2017.09.020_bib0065","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.pt.2014.12.012","article-title":"The past, present, and future of Leishmania genomics and transcriptomics","volume":"31","author":"Cantacessi","year":"2015","journal-title":"Trends Parasitol."},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0070","doi-asserted-by":"crossref","first-page":"122","DOI":"10.4269\/ajtmh.2008.78.122","article-title":"A real-time polymerase chain reaction assay for the identification and quantification of American Leishmania species on the basis of glucose-6-phosphate dehydrogenase","volume":"78","author":"Castilho","year":"2008","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"2","key":"10.1016\/j.actatropica.2017.09.020_bib0075","doi-asserted-by":"crossref","first-page":"e88845","DOI":"10.1371\/journal.pone.0088845","article-title":"Detection and characterization of Leishmania (Leishmania) and Leishmania (Viannia) by SYBR green-based real-time PCR and high resolution melt analysis targeting kinetoplast minicircle DNA","volume":"9","author":"Ceccarelli","year":"2014","journal-title":"PLoS One"},{"issue":"3","key":"10.1016\/j.actatropica.2017.09.020_bib0080","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/S0001-706X(99)00005-4","article-title":"Polymerase chain reaction reveals Trypanosoma cruzi infection suspected by serology in cutaneous and mucocutaneous leishmaniasis patients","volume":"72","author":"Chiaramonte","year":"1999","journal-title":"Acta Trop."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0085","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.jviromet.2014.11.011","article-title":"Development and application of a real-time PCR assay for the detection and quantitation of lymphocystis disease virus","volume":"213","author":"Ciulli","year":"2015","journal-title":"J. Virol. Methods"},{"issue":"Suppl. (1)","key":"10.1016\/j.actatropica.2017.09.020_bib0090","doi-asserted-by":"crossref","first-page":"S173","DOI":"10.1016\/S0035-9203(02)90072-6","article-title":"Parasitological and immunological follow-up of American tegumentary leishmaniasis patients","volume":"96","author":"Coutinho","year":"2002","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"2","key":"10.1016\/j.actatropica.2017.09.020_bib0095","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1017\/S0950268800057964","article-title":"Cutaneous leishmaniasis in the Peruvian Andes: an epidemiological study of infection and immunity","volume":"114","author":"Davies","year":"1995","journal-title":"Epidemiol. Infect."},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0100","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1128\/JCM.01764-16","article-title":"Detection and differentiation of Leishmania spp. in clinical specimens by use of a SYBR Green-based real-time PCR assay","volume":"55","author":"De Almeida","year":"2016","journal-title":"J. Clin. Microbiol."},{"issue":"4","key":"10.1016\/j.actatropica.2017.09.020_bib0105","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1017\/S0031182012001886","article-title":"Mixed infection in the anteater Tamandua tetradactyla (Mammalia: Pilosa) from Par\u00e1 State, Brazil: Trypanosoma cruzi, T. rangeli and Leishmania infantum","volume":"140","author":"De Ara\u00fajo","year":"2013","journal-title":"Parasitology"},{"key":"10.1016\/j.actatropica.2017.09.020_bib0110","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.exppara.2016.03.005","article-title":"Real-time PCR for Leishmania species identification: evaluation and comparison with classical techniques","volume":"165","author":"De Morais","year":"2016","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0115","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.actatropica.2004.08.002","article-title":"Rapid clearance of circulating Leishmania kinetoplast DNA after treatment of visceral leishmaniasis","volume":"92","author":"Disch","year":"2004","journal-title":"Acta Trop."},{"issue":"7","key":"10.1016\/j.actatropica.2017.09.020_bib0120","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1128\/JCM.02555-06","article-title":"Detection and identification of Leishmania species from clinical specimens by using a real-time PCR assay and sequencing of the cytochrome B gene","volume":"45","author":"Foulet","year":"2007","journal-title":"J. Clin. Microbiol."},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0125","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1017\/S0031182002002585","article-title":"Characterization of human infection by Leishmania spp. in the Northwest of Argentina: immune response, double infection with Trypanosoma cruzi and species of Leishmania involveds","volume":"126","author":"Frank","year":"2003","journal-title":"Parasitology"},{"issue":"Pt. 2","key":"10.1016\/j.actatropica.2017.09.020_bib0130","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1258\/000456304322880014","article-title":"Genotyping of hepatitis C virus by melting curve analysis with SYBR Green I","volume":"41","author":"Fujigaki","year":"2004","journal-title":"Ann. Clin. Biochem."},{"issue":"5","key":"10.1016\/j.actatropica.2017.09.020_bib0135","doi-asserted-by":"crossref","first-page":"2294","DOI":"10.1128\/JCM.42.5.2294-2297.2004","article-title":"Culture-independent species typing of neotropical Leishmania for clinical validation of a PCR-based assay targeting heat shock protein 70 genes","volume":"42","author":"Garcia","year":"2004","journal-title":"J. Clin. Microbiol."},{"issue":"5","key":"10.1016\/j.actatropica.2017.09.020_bib0140","first-page":"420","article-title":"Role of three ELISA tests using promastigote homogenates of Leishmania braziliensis, L. amazonensis and L. guyanensis in the diagnosis of tegumentary leishmaniasis","volume":"71","author":"Gil","year":"2011","journal-title":"Medicina (Mex)."},{"issue":"3\u20134","key":"10.1016\/j.actatropica.2017.09.020_bib0145","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.vetpar.2006.10.008","article-title":"PCR identification of Leishmania in diagnosis and control of canine Leishmaniasis","volume":"144","author":"Gomes","year":"2007","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0150","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1128\/JCM.01954-16","article-title":"Field validation of SYBR Green- and TaqMan-based real-time PCR using biopsy and swab samples to diagnose American tegumentary leishmaniasis in an area where Leishmania (Viannia) braziliensis is endemic","volume":"55","author":"Gomes","year":"2017","journal-title":"J. Clin. Microbiol."},{"issue":"5","key":"10.1016\/j.actatropica.2017.09.020_bib0155","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1590\/S0074-02762012000500014","article-title":"Development and validation of PCR-based assays for diagnosis of American cutaneous leishmaniasis and identification of the parasite species","volume":"107","author":"Gra\u00e7a","year":"2012","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.actatropica.2017.09.020_bib0160","doi-asserted-by":"crossref","first-page":"281","DOI":"10.4269\/ajtmh.1991.45.281","article-title":"Correlation between histopathology, immune response, clinical presentation, and evolution in Leishmania braziliensis infection","volume":"45","author":"Gutierrez","year":"1991","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0165","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1186\/s13071-014-0501-y","article-title":"Identification of Six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay","volume":"7","author":"Hern\u00e1ndez","year":"2014","journal-title":"Parasit Vectors"},{"issue":"9185","key":"10.1016\/j.actatropica.2017.09.020_bib0170","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1016\/S0140-6736(98)10178-2","article-title":"Leishmaniasis","volume":"354","author":"Herwaldt","year":"1999","journal-title":"Lancet"},{"key":"10.1016\/j.actatropica.2017.09.020_bib0175","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1146\/annurev-med-051214-024241","article-title":"New vaccines for the world\u2019s poorest people","volume":"67","author":"Hotez","year":"2016","journal-title":"Annu. Rev. Med."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0180","doi-asserted-by":"crossref","first-page":"6456031","DOI":"10.1155\/2016\/6456031","article-title":"Epidemiology of american tegumentary leishmaniasis and trypanosoma cruzi infection in the northwestern Argentina","volume":"2016","author":"Hoyos","year":"2016","journal-title":"BioMed Res. Int."},{"issue":"10","key":"10.1016\/j.actatropica.2017.09.020_bib0185","doi-asserted-by":"crossref","first-page":"3712","DOI":"10.1128\/JCM.00843-06","article-title":"Staphylococcus aureus genotyping using novel real-time PCR formats","volume":"44","author":"Huygens","year":"2006","journal-title":"J. Clin. Microbiol."},{"issue":"6","key":"10.1016\/j.actatropica.2017.09.020_bib0190","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.1128\/JCM.00208-13","article-title":"Real-time PCR assay for detection and quantification of Leishmania (Viannia) organisms in skin and mucosal lesions: exploratory study of parasite load and clinical parameters","volume":"51","author":"Jara","year":"2013","journal-title":"J. Clin. Microbiol."},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0195","doi-asserted-by":"crossref","first-page":"107","DOI":"10.4269\/ajtmh.15-0514","article-title":"Quantitative kinetoplast DNA assessment during treatment of mucosal leishmaniasis as a potential biomarker of outcome: a pilot study","volume":"94","author":"Jara","year":"2016","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0200","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1016\/j.micinf.2005.04.009","article-title":"Analysis of ribosomal DNA internal transcribed spacer sequences of the Leishmania donovani complex","volume":"7","author":"Kuhls","year":"2005","journal-title":"Microbes Infect."},{"issue":"3\u20134","key":"10.1016\/j.actatropica.2017.09.020_bib0205","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.vetpar.2013.03.017","article-title":"Asymptomatic dogs are highly competent to transmit Leishmania (Leishmania) infantum chagasi to the natural vector","volume":"196","author":"Laurenti","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0210","doi-asserted-by":"crossref","first-page":"348","DOI":"10.4269\/ajtmh.1993.49.348","article-title":"Diagnosis of Leishmania using the polymerase chain reaction: a simplified procedure for field work","volume":"49","author":"Lopez","year":"1993","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0215","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/EC.1.4.495-502.2002","article-title":"Kinetoplast DNA network: evolution of an improbable structure","volume":"1","author":"Luke\u0161","year":"2002","journal-title":"Eukaryot. Cell"},{"issue":"7","key":"10.1016\/j.actatropica.2017.09.020_bib0220","doi-asserted-by":"crossref","first-page":"3147","DOI":"10.1128\/JCM.41.7.3147-3153.2003","article-title":"Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis","volume":"41","author":"Marfurt","year":"2003","journal-title":"J. Clin. Microbiol."},{"issue":"7","key":"10.1016\/j.actatropica.2017.09.020_bib0225","doi-asserted-by":"crossref","first-page":"e0005713","DOI":"10.1371\/journal.pntd.0005713","article-title":"Clinical and parasitological factors in parasite persistence after treatment and clinical cure of cutaneous leishmaniasis","volume":"11","author":"Mart\u00ednez-Valencia","year":"2017","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0230","doi-asserted-by":"crossref","first-page":"5249","DOI":"10.1128\/JCM.42.11.5249-5255.2004","article-title":"Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity","volume":"42","author":"Mary","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0235","doi-asserted-by":"crossref","first-page":"858","DOI":"10.4269\/ajtmh.2006.75.858","article-title":"Reference values for Leishmania infantum parasitemia in different clinical presentations: quantitative polymerase chain reaction for therapeutic monitoring and patient follow-up","volume":"75","author":"Mary","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0240","doi-asserted-by":"crossref","first-page":"3038","DOI":"10.1128\/JCM.43.7.3038-3041.2005","article-title":"Evaluation of PCR for diagnosis of Indian kala-azar and assessment of cure","volume":"43","author":"Maurya","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0245","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1086\/382135","article-title":"Persistence of Leishmania parasites in scars after clinical cure of American cutaneous leishmaniasis: is there a sterile cure","volume":"189","author":"Mendon\u00e7a","year":"2004","journal-title":"J. Infect. Dis."},{"issue":"1\u20132","key":"10.1016\/j.actatropica.2017.09.020_bib0250","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.vetpar.2007.03.004","article-title":"Infectivity of seropositive dogs, showing different clinical forms of leishmaniasis, to Lutzomyia longipalpis phlebotomine sand flies","volume":"147","author":"Michalsky","year":"2007","journal-title":"Vet. Parasitol."},{"issue":"4","key":"10.1016\/j.actatropica.2017.09.020_bib0255","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/0035-9203(94)90446-4","article-title":"Infectivity of dogs naturally infected with Leishmania infantum to colonized Phlebotomus perniciosus","volume":"88","author":"Molina","year":"1994","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0260","doi-asserted-by":"crossref","first-page":"105","DOI":"10.4269\/ajtmh.12-0527","article-title":"Ultrasensitive real-time PCR for the clinical management of visceral leishmaniasis in HIV-Infected patients","volume":"89","author":"Molina","year":"2013","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"3","key":"10.1016\/j.actatropica.2017.09.020_bib0265","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.jcv.2012.11.003","article-title":"Detection and genotyping of human papillomavirus by real-time PCR assay","volume":"56","author":"Moreau","year":"2013","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0270","first-page":"954","article-title":"Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification","volume":"24","author":"Morrison","year":"1998","journal-title":"Biotechniques"},{"issue":"9","key":"10.1016\/j.actatropica.2017.09.020_bib0275","doi-asserted-by":"crossref","first-page":"2848","DOI":"10.1128\/JCM.00951-08","article-title":"Molecular diagnosis of Old World cutaneous leishmaniasis and species identification by use of a reverse line blot hybridization assay","volume":"46","author":"Nasereddin","year":"2008","journal-title":"J. Clin. Microbiol."},{"issue":"9","key":"10.1016\/j.actatropica.2017.09.020_bib0280","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1186\/s13071-016-1531-4","article-title":"Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation","volume":"3","author":"Nath-Chowdhury","year":"2016","journal-title":"Parasit Vectors"},{"key":"10.1016\/j.actatropica.2017.09.020_bib0285","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.cca.2014.10.017","article-title":"Real-time PCR detection chemistry","volume":"439","author":"Navarro","year":"2015","journal-title":"Clin. Chim. Acta"},{"key":"10.1016\/j.actatropica.2017.09.020_bib0290","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1093\/infdis\/171.3.751","article-title":"Diagnosis of symptomatic visceral leishmaniasis by use of the polymerase chain reaction on patient blood","volume":"171","author":"Nuzum","year":"1995","journal-title":"J. Infect. Dis."},{"issue":"12","key":"10.1016\/j.actatropica.2017.09.020_bib0295","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1016\/j.trstmh.2006.02.014","article-title":"Haematogenous dissemination of Leishmania (Viannia) braziliensis in human American tegumentary leishmaniasis","volume":"100","author":"Oliveira Camera","year":"2006","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"2","key":"10.1016\/j.actatropica.2017.09.020_bib0300","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jviromet.2004.04.012","article-title":"Simultaneous quantitation and genotyping of hepatitis B virus by real-time PCR and melting curve analysis","volume":"120","author":"Payungporn","year":"2004","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.actatropica.2017.09.020_bib0305","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1038\/ng2053","article-title":"Comparative genomic analysis of three Leishmania species that cause diverse human disease","volume":"39","author":"Peacock","year":"2007","journal-title":"Nat. Genet."},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0310","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1590\/0037-8682-0191-2015","article-title":"Detection of canine visceral leishmaniasis by conjunctival swab PCR","volume":"49","author":"Pereira","year":"2016","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0315","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1756-3305-5-15","article-title":"SYBR green-based real-time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniases","volume":"5","author":"Pita-Pereira","year":"2012","journal-title":"Parasit Vectors"},{"issue":"11","key":"10.1016\/j.actatropica.2017.09.020_bib0320","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1016\/j.micinf.2007.06.005","article-title":"Leishmania DNA is rapidly degraded following parasite death: an analysis by microscopy and real-time PCR","volume":"9","author":"Prina","year":"2007","journal-title":"Microb. Infect."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0325","doi-asserted-by":"crossref","first-page":"3768","DOI":"10.1128\/JCM.38.10.3768-3773.2000","article-title":"Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitologic diagnosis","volume":"38","author":"Ram\u00edrez","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0330","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1093\/dnares\/dst031","article-title":"The genome sequence of Leishmania (Leishmania) amazonensis: functional annotation and extended analysis of gene models","volume":"20","author":"Real","year":"2013","journal-title":"DNA Res."},{"issue":"6","key":"10.1016\/j.actatropica.2017.09.020_bib0335","doi-asserted-by":"crossref","first-page":"597","DOI":"10.2217\/14622416.8.6.597","article-title":"High-resolution DNA melting analysis for simple and efficient molecular diagnostics","volume":"8","author":"Reed","year":"2007","journal-title":"Pharmacogenomics"},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0340","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1128\/JCM.02029-06","article-title":"Molecular diagnosis of leishmaniasis: current status and future applications","volume":"45","author":"Reithinger","year":"2007","journal-title":"J. Clin. Microbiol."},{"issue":"2","key":"10.1016\/j.actatropica.2017.09.020_bib0345","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1006\/abio.1996.9916","article-title":"Product differentiation by analysis of DNA melting curves during the polymerase chain reaction","volume":"245","author":"Ririe","year":"1997","journal-title":"Anal. Biochem."},{"issue":"Suppl. (1)","key":"10.1016\/j.actatropica.2017.09.020_bib0350","first-page":"S169","article-title":"The method used to sample ulcers influences the diagnosis of cutaneous leishmaniasis","volume":"96","author":"Robinson","year":"2002","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"9","key":"10.1016\/j.actatropica.2017.09.020_bib0355","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1086\/319990","article-title":"Comparison of cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis and L. (V.) guyanensis in Brazil: clinical findings and diagnostic approach","volume":"32","author":"Romero","year":"2001","journal-title":"Clin. Infect. Dis."},{"issue":"9","key":"10.1016\/j.actatropica.2017.09.020_bib0360","doi-asserted-by":"crossref","first-page":"e819","DOI":"10.1371\/journal.pntd.0000819","article-title":"Viability and burden of leishmania in extralesional sites during human dermal leishmaniasis","volume":"4","author":"Romero","year":"2010","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"12","key":"10.1016\/j.actatropica.2017.09.020_bib0365","doi-asserted-by":"crossref","first-page":"e0004273","DOI":"10.1371\/journal.pntd.0004273","article-title":"Parasitological confirmation and analysis of leishmania diversity in asymptomatic and subclinical infection following resolution of cutaneous leishmaniasis","volume":"9","author":"Rosales-Chilama","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0370","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0035-9203(01)90173-7","article-title":"Genetic heterogeneity in the species Leishmania tropica revealed by different PCR-based methods","volume":"95","author":"Sch\u00f6nian","year":"2001","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0375","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0732-8893(03)00093-2","article-title":"PCR diagnosis and characterization of Leishmania in local and imported clinical samples","volume":"47","author":"Sch\u00f6nian","year":"2003","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0380","first-page":"394","article-title":"Molecular tools and strategies for diagnosis of chagas disease and leishmaniasis","volume":"2016","author":"Schijman","year":"2016","journal-title":"Front. Parasitol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0385","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1086\/515355","article-title":"Detection of Leishmania DNA by polymerase chain reaction in scars of treated human patients","volume":"178","author":"Schubach","year":"1998","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0390","doi-asserted-by":"crossref","first-page":"824","DOI":"10.4269\/ajtmh.1998.58.824","article-title":"Cutaneous scars in American tegumentary leishmaniasis patients: a site of Leishmania (Viannia) braziliensis persistence and viability eleven years after antimonial therapy and clinical cure","volume":"58","author":"Schubach","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0395","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0074-7696(08)61426-6","article-title":"Kinetoplast DNA in trypanosomid flagellates","volume":"99","author":"Simpson","year":"1986","journal-title":"Int. Rev. Cytol."},{"issue":"10","key":"10.1016\/j.actatropica.2017.09.020_bib0400","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1093\/cid\/ciu102","article-title":"Asymptomatic Leishmania infection: a new challenge for Leishmania control","volume":"58","author":"Singh","year":"2014","journal-title":"Clin. Infect. Dis."},{"issue":"7","key":"10.1016\/j.actatropica.2017.09.020_bib0405","doi-asserted-by":"crossref","first-page":"e0003936","DOI":"10.1371\/journal.pntd.0003936","article-title":"Quantification of leishmania (Viannia) kinetoplast DNA in ulcers of cutaneous leishmaniasis reveals inter-site and inter-sampling variability in parasite load","volume":"23","author":"Su\u00e1rez","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"9","key":"10.1016\/j.actatropica.2017.09.020_bib0410","doi-asserted-by":"crossref","first-page":"3237","DOI":"10.1128\/JCM.40.9.3237-3244.2002","article-title":"Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis","volume":"40","author":"Tanriverdi","year":"2002","journal-title":"J. Clin. Microbiol. Sep."},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0415","doi-asserted-by":"crossref","first-page":"e1956","DOI":"10.1371\/journal.pntd.0001956","article-title":"A FRET-based real-time PCR assay to identify the main causal agents of New World tegumentary leishmaniasis","volume":"7","author":"Tsukayama","year":"2013","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"11","key":"10.1016\/j.actatropica.2017.09.020_bib0420","doi-asserted-by":"crossref","first-page":"e49738","DOI":"10.1371\/journal.pone.0049738","article-title":"Non-invasive cytology brush PCR for the diagnosis and causative species identification of American cutaneous leishmaniasis in Peru","volume":"7","author":"Valencia","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.actatropica.2017.09.020_bib0425","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0166-6851(92)90208-2","article-title":"Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites","volume":"51","author":"Van Eys","year":"1992","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0430","doi-asserted-by":"crossref","first-page":"5560","DOI":"10.1128\/JCM.43.11.5560-5566.2005","article-title":"Quantitative nucleic acid sequence-based assay as a new molecular tool for the detection and quantification of Leishmania parasites in skin biopsies","volume":"43","author":"Van der Meide","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0435","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1111\/j.1365-2230.2007.02680.x","article-title":"Treatment assessment by monitoring parasite load in skin biopsies from patients with cutaneous leishmaniasis, using quantitative nucleic acid sequence-based amplification","volume":"33","author":"Van der Meide","year":"2008","journal-title":"Clin. Exp. Dermatol."},{"issue":"9","key":"10.1016\/j.actatropica.2017.09.020_bib0440","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1111\/tmi.12144","article-title":"Performance of different Trypanosoma cruzi antigens in the diagnosis of Chagas disease in patients with American cutaneous leishmaniasis from a co-endemic region in Argentina","volume":"18","author":"Vega Benedetti","year":"2013","journal-title":"Trop. Med. Int. Health"},{"issue":"4","key":"10.1016\/j.actatropica.2017.09.020_bib0445","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1086\/505583","article-title":"Evidence for leishmania (viannia) parasites in the skin and blood of patients before and after treatment","volume":"194","author":"Vergel","year":"2006","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0450","series-title":"Control of the Leishmaniases: Report of a Meeting of the WHO Expert Committee on the Control of Leishmaniases","first-page":"949","author":"World Health Organization","year":"2010"},{"key":"10.1016\/j.actatropica.2017.09.020_bib0455","series-title":"Research Priorities for Chagas Disease, Human African Trypanosomiasis and Leishmaniasis. Technical Report of the TDR Disease Reference Group on Chagas Disease, Human African Trypanosomiasis and Leishmaniasis","author":"World Health Organization","year":"2012"},{"key":"10.1016\/j.actatropica.2017.09.020_bib0460","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1093\/infdis\/168.3.699","article-title":"Epidemiology of cutaneous leishmaniasis in Colombia: a longitudinal study of the natural history, prevalence, and incidence of infection and clinical manifestations","volume":"168","author":"Weigle","year":"1993","journal-title":"J. Infect. Dis."},{"issue":"2","key":"10.1016\/j.actatropica.2017.09.020_bib0465","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1128\/JCM.40.2.601-606.2002","article-title":"PCR-based diagnosis of acute and chronic cutaneous leishmaniasis caused by Leishmania (Viannia)","volume":"40","author":"Weigle","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0470","doi-asserted-by":"crossref","first-page":"3892","DOI":"10.1128\/JCM.r00764-11","article-title":"Serial quantitative PCR assay for detection: species discrimination and quantification of Leishmania spp. in human samples","volume":"49","author":"Weirather","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2017.09.020_bib0475","doi-asserted-by":"crossref","first-page":"130","DOI":"10.2144\/97221bi01","article-title":"Continuous fluorescence monitoring of rapid cycle DNA amplification","volume":"22","author":"Wittwer","year":"1997","journal-title":"Biotechniques"},{"issue":"12","key":"10.1016\/j.actatropica.2017.09.020_bib0480","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.1002\/jmv.23955","article-title":"A novel molecular test for influenza B virus detection and lineage differentiation","volume":"86","author":"Wong","year":"2014","journal-title":"J. Med. Virol."},{"issue":"1","key":"10.1016\/j.actatropica.2017.09.020_bib0485","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s00705-016-3059-5","article-title":"A simple real-time polymerase chain reaction assay using SYBR Green for hepatitis C virus genotyping","volume":"162","author":"Zaghloul","year":"2017","journal-title":"Arch. Virol."},{"issue":"2","key":"10.1016\/j.actatropica.2017.09.020_bib0490","doi-asserted-by":"crossref","first-page":"e0004485","DOI":"10.1371\/journal.pntd.0004485","article-title":"High resolution melting analysis targeting hsp70 as a fast and efficient method for the discrimination of Leishmania species","volume":"10","author":"Zampieri","year":"2016","journal-title":"PLoS Negl. Trop. Dis."}],"container-title":["Acta Tropica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X17304850?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X17304850?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,18]],"date-time":"2020-10-18T13:54:25Z","timestamp":1603029265000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0001706X17304850"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":98,"alternative-id":["S0001706X17304850"],"URL":"https:\/\/doi.org\/10.1016\/j.actatropica.2017.09.020","relation":{},"ISSN":["0001-706X"],"issn-type":[{"value":"0001-706X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The applicability of real-time PCR in the diagnostic of cutaneous leishmaniasis and parasite quantification for clinical management: Current status and perspectives","name":"articletitle","label":"Article Title"},{"value":"Acta Tropica","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actatropica.2017.09.020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}