{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:36:03Z","timestamp":1788568563078,"version":"build-2803163510"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Federal Government of Brazil"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Tropica"],"published-print":{"date-parts":[[2018,3]]},"DOI":"10.1016\/j.actatropica.2017.12.028","type":"journal-article","created":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T17:27:43Z","timestamp":1514568463000},"page":"81-87","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"special_numbering":"C","title":["Serological detection and molecular characterization of piroplasmids in equids in Brazil"],"prefix":"10.1016","volume":"179","author":[{"given":"Maria Isabel Botelho","family":"Vieira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcio Machado","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus Tonial","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Ricardo","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosangela Zacarias","family":"Machado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.actatropica.2017.12.028_bib0005","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1196\/annals.1373.076","article-title":"Molecular sequence evidence for the reclassification of some Babesia species","volume":"1081","author":"Allsopp","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0010","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0015","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1590\/S1984-29612010000400007","article-title":"Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, S\u00e3o Paulo State, Brazil","volume":"19","author":"Baldani","year":"2010","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0020","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0304-4017(02)00131-0","article-title":"Detection of natural infection of Boophilus microplus with Babesia equi and Babesia caballi in Brazilian horses using nested polymerase chain reaction","volume":"107","author":"Battsetseg","year":"2002","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0025","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.vetpar.2008.10.004","article-title":"Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa","volume":"159","author":"Bhoora","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0030","doi-asserted-by":"crossref","first-page":"141","DOI":"10.4067\/S0717-77122002000300010","article-title":"Seroprevalencia de Babesia equi en tres diferentes sistemas de crianza de equinos: Rio de Janeiro-Brasil","volume":"57","author":"Botteon","year":"2002","journal-title":"Parasitol. Latinoam."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0035","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0007-1935(96)80070-4","article-title":"Equine piroplasmosis an update on diagnosis, treatment and prevention","volume":"152","author":"Br\u00fcning","year":"1996","journal-title":"Br. Vet. J."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0040","series-title":"IV Simp\u00f3sio Brasileiro de Acarologia","article-title":"Soropreval\u00eancia de Theileria equi em equinos do munic\u00edpio de Passo Fundo, RS","author":"Dall\u2019Agnol","year":"2013"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0045","series-title":"Clima de Passo Fundo","author":"Embrapa","year":"2017"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0050","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.cimid.2016.06.004","article-title":"Serological and molecular detection of Theileria equi in sport horses of northeastern Brazil","volume":"47","author":"Ferreira","year":"2016","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0055","series-title":"Proceedings of the Eight International Conference on Equine Infectious Diseases","first-page":"217","article-title":"Mechanical transmission of Babesia equi infection in a British herd of horses","author":"Getstenberg","year":"1999"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0060","first-page":"317","article-title":"Seroepidemiological studies on Babesia equi in horses from the State of Rio Grande do Sul determined by indirect immunoflourecence test and Elisa","volume":"17","author":"Golynski","year":"2008","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0065","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/s004360050404","article-title":"Sporogony and experimental transmission of Babesia equi by Boophilus microplus","volume":"84","author":"Guimar\u00e3es","year":"1998","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0070","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-6-35","article-title":"Genetic characterization of Theileria equi infecting horses in North America: evidence for a limited source of US introductions","volume":"6","author":"Hall","year":"2013","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0075","series-title":"Nucleic Acids Symposium Series","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","author":"Hall","year":"1999"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0080","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s00436-007-0726-1","article-title":"Detection and molecular characterization of Babesia caballi and Theileria equi isolates from endemic areas of Brazil","volume":"102","author":"Heim","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0085","series-title":"Sumariza\u00e7\u00e3o dos registros de esp\u00e9cies e hospedeiros para material do Rio Grande do Sul da COLE\u00c7\u00c3O DE CARRAPATOS do Laborat\u00f3rio de Parasitologia do IPVDF","author":"IPVDF","year":"2013"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0090","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2164-13-603","article-title":"Comparative genomic analysis and phylogenetic position of Theileria equi","volume":"13","author":"Kappmeyer","year":"2012","journal-title":"BMC Genomics"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0095","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4322\/rbpv.01804001","article-title":"Prevalence of equine Piroplasmosis and its association with tick infestation in the State of S\u00e3o Paulo, Brazil","volume":"18","author":"Kerber","year":"2009","journal-title":"Rev. Bras. Parasitol. Vet."},{"issue":"4","key":"10.1016\/j.actatropica.2017.12.028_bib0100","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.ttbdis.2017.02.010","article-title":"Characterization of Theileria equi genotypes in horses in Israel, the Palestinian Authority and Jordan","volume":"8","author":"Ketter-Ratzon","year":"2017","journal-title":"Ticks Tick Borne Dis."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0105","first-page":"385","article-title":"Contribution a l'etude de Piroplasma equi","volume":"53","author":"Laveran","year":"1901","journal-title":"CR. Soc. Biol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0110","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0304-4017(97)00003-4","article-title":"An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Babesia bovis in cattle","volume":"71","author":"Machado","year":"1997","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0115","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.vetpar.2011.11.069","article-title":"Molecular and serological detection of Theileria equi and Babesia caballi in donkeys (Equus asinus) in Brazil","volume":"186","author":"Machado","year":"2012","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0120","doi-asserted-by":"crossref","first-page":"319","DOI":"10.11606\/issn.1678-4456.v52i4p319-324","article-title":"Ocorr\u00eancia de carrapatos em animais silvestres recebidos e atendidos pelo Parque Zool\u00f3gico Municipal Quinzinho de Barros, Sorocaba, S\u00e3o Paulo, Brasil","volume":"52","author":"Martins","year":"2015","journal-title":"Braz. J. Vet. Res. An. Sci."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0125","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.vetpar.2008.06.025","article-title":"Detection of a Theileria species in dogs in South Africa","volume":"157","author":"Matjila","year":"2008","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0130","first-page":"41","article-title":"The CIPRES science gateway: a community resource for phylogenetic analyses","author":"Miller","year":"2011","journal-title":"Proceedings of the 2011 TeraGrid Conference: Extreme Digital Discovery"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0135","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.meegid.2013.02.005","article-title":"Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia","volume":"16","author":"Munkhjargal","year":"2013","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0140","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.vetpar.2004.04.010","article-title":"Detection and identification of equine Theileria and Babesia species by reverse line blotting: epidemiological survey and phylogenetic analysis","volume":"123","author":"Nagore","year":"2004","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0145","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0304-4017(01)00471-X","article-title":"Detection of Babesia equi (Laveran, 1901) by nested polymerase chain reaction","volume":"101","author":"Nicolaiewsky","year":"2001","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0150","first-page":"46","article-title":"Frequency of seropositive equines for Theileria equi in the southern rio grande do sul state, Brazil","volume":"63","author":"Nizoli","year":"2008","journal-title":"Parasitol. Latinoam."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0155","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1017\/S003118200000010X","article-title":"On the occurrence of two species of parasites in equine piroplasmosis or biliary fever","volume":"5","author":"Nuttall","year":"1912","journal-title":"Parasitology"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0160","series-title":"Equine Piroplasmosis. Terrestrial Manual","article-title":"Chapter 2.5.8","author":"OIE","year":"2014"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0165","series-title":"Piroplasmose equina. C\u00f3digo Sanitario para los Animales Terrestres","article-title":"Cap\u00edtulo 12.7","author":"OIE","year":"2017"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0170","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1128\/JCM.00167-08","article-title":"Identification of a novel Babesia sp. from a sable antelope (Hippotragus niger Harris, 1838)","volume":"46","author":"Oosthuizen","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0175","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1590\/S1984-29612014012","article-title":"Seroprevalence rates of antibodies against Theileria equi in team roping horses from central-western region of Paran\u00e1","volume":"23","author":"Prochno","year":"2014","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0180","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1017\/S0031182013000486","article-title":"Infections by Babesia caballi and Theileria equi in Jordanian equids: epidemiology and genetic diversity","volume":"140","author":"Qablan","year":"2013","journal-title":"Parasitology"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0185","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.exppara.2011.03.016","article-title":"Failure of the Amblyomma cajennense nymph to become infected by Theileria equi after feeding on acute or chronically infected horses","volume":"128","author":"Ribeiro","year":"2011","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0190","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.meegid.2013.03.038","article-title":"Prevalence and genetic diversity of piroplasm species in horses and ticks from Tunisia","volume":"17","author":"Ros-Garc\u00eda","year":"2013","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0195","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1007\/s00436-009-1691-7","article-title":"Nucleotide sequence heterogeneity in the small subunit ribosomal RNA gene within Theileria equi from horses in Sudan","volume":"106","author":"Salim","year":"2010","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0200","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1186\/1756-3305-6-306","article-title":"Amblyomma cajennense is an intrastadial biological vector of Theileria equi","volume":"6","author":"Scoles","year":"2013","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0205","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.rvsc.2013.01.014","article-title":"Molecular and phylogenetic analysis of equine piroplasms in the Republic of Korea","volume":"94","author":"Seo","year":"2013","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0210","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1093\/bioinformatics\/bti191","article-title":"RAxML-III: a fast program for maximum likelihood-based inference of large phylogenetic trees","volume":"21","author":"Stamatakis","year":"2005","journal-title":"Bioinformatics"},{"key":"10.1016\/j.actatropica.2017.12.028_bib0215","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1603\/0022-2585-39.4.667","article-title":"Dermacentor variabilis and Boophilus microplus (Acari: ixodidae): experimental vectors of Babesia equi to equids","volume":"39","author":"Stiller","year":"2002","journal-title":"J. Med. Entomol."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0220","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0225","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1111\/jvim.12168","article-title":"Review of equine piroplasmosis","volume":"27","author":"Wise","year":"2013","journal-title":"J. Vet. Intern. Med."},{"key":"10.1016\/j.actatropica.2017.12.028_bib0230","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.jevs.2008.03.005","article-title":"Clinical and laboratory findings in equine piroplasmosis","volume":"28","author":"Zobba","year":"2008","journal-title":"J. Eq. Vet. Sci."}],"container-title":["Acta Tropica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X17310264?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X17310264?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,7,10]],"date-time":"2018-07-10T06:12:56Z","timestamp":1531203176000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0001706X17310264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3]]},"references-count":46,"alternative-id":["S0001706X17310264"],"URL":"https:\/\/doi.org\/10.1016\/j.actatropica.2017.12.028","relation":{},"ISSN":["0001-706X"],"issn-type":[{"value":"0001-706X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Serological detection and molecular characterization of piroplasmids in equids in Brazil","name":"articletitle","label":"Article Title"},{"value":"Acta Tropica","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actatropica.2017.12.028","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}