{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T15:55:42Z","timestamp":1780761342527,"version":"3.54.1"},"reference-count":53,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Tropica"],"published-print":{"date-parts":[[2018,6]]},"DOI":"10.1016\/j.actatropica.2018.02.021","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T02:23:03Z","timestamp":1519179783000},"page":"100-110","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Comparative genomic analysis of Rickettsia rickettsii for identification of drug and vaccine targets: tolC as a proposed candidate for case study"],"prefix":"10.1016","volume":"182","author":[{"given":"Pramod Kumar","family":"Maurya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swati","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashutosh","family":"Mani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.actatropica.2018.02.021_bib0005","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0010","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s12154-010-0039-1","article-title":"In silico identification of common putative drug targets in Leptospira interrogans","volume":"3","author":"Amineni","year":"2010","journal-title":"J. Chem. Biol."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0015","first-page":"284170","article-title":"Mycobacterium tuberculosis H37Rv: In silico drug targets identification by metabolic pathways analysis","author":"Amir","year":"2014","journal-title":"Int. J. Evol. Biol."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0020","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1002\/ddr.20413","article-title":"In silico substractive genomics for target identification in human bacterial pathogens","volume":"72","author":"Barh","year":"2011","journal-title":"Drug Dev. Res."},{"issue":"10","key":"10.1016\/j.actatropica.2018.02.021_bib0025","doi-asserted-by":"crossref","first-page":"3729","DOI":"10.1128\/AAC.48.10.3729-3735.2004","article-title":"AcrAB-tolC directs efflux-mediated multidrug resistance in salmonella enterica serovar typhimurium DT104","volume":"48","author":"Baucheron","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"issue":"July (5016)","key":"10.1016\/j.actatropica.2018.02.021_bib0030","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1126\/science.1853201","article-title":"A method to identify protein sequences that fold into a known three-dimensional structure","volume":"253","author":"Bowie","year":"1991","journal-title":"Science"},{"issue":"5","key":"10.1016\/j.actatropica.2018.02.021_bib0035","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1111\/j.1462-5822.2005.00671.x","article-title":"The AcrAB-TolC efflux system of Salmonella enterica serovar Typhimurium plays a role in pathogenesis","volume":"8","author":"Buckley","year":"2006","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0040","first-page":"3","article-title":"Distribucion de las rickettsiasis en Mexico","volume":"8","author":"Bustamante","year":"1947","journal-title":"Rev. Inst. Salubr. Enferm. Trop."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0045","doi-asserted-by":"crossref","first-page":"42717","DOI":"10.1038\/srep42717","article-title":"SwissADME: a free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules","volume":"7","author":"Daina","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0050","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/978-1-4939-2269-7_19","article-title":"Small-molecule library screening by docking with PyRx","volume":"1263","author":"Dallakyan","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0055","doi-asserted-by":"crossref","first-page":"405","DOI":"10.4269\/ajtmh.1995.52.405","article-title":"National surveillance for Rocky Mountain spotted fever: epidemiologic summary and evaluation of risk factors for fatal outcome","volume":"52","author":"Dalton","year":"1995","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"11","key":"10.1016\/j.actatropica.2018.02.021_bib0060","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/S1473-3099(07)70261-X","article-title":"Rocky mountain spotted fever","volume":"7","author":"Dantas-Torres","year":"2007","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0065","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1056\/NEJMoa050043","article-title":"Rocky Mountain spotted fever from an unexpected tick vector in Arizona","volume":"353","author":"Demma","year":"2005","journal-title":"N. Engl. J. Med."},{"issue":"1\u20132","key":"10.1016\/j.actatropica.2018.02.021_bib0070","doi-asserted-by":"crossref","first-page":"43","DOI":"10.3233\/ISB-00220","article-title":"In silico identification of potential therapeutic targets in the human pathogen Helicobacter pylori","volume":"6","author":"Dutta","year":"2006","journal-title":"In Silico Biol."},{"issue":"December (18)","key":"10.1016\/j.actatropica.2018.02.021_bib0075","doi-asserted-by":"crossref","first-page":"2500","DOI":"10.1093\/bioinformatics\/btg362","article-title":"ModLoop: automated modeling of loops in protein structures","volume":"19","author":"Fiser","year":"2003","journal-title":"Bioinformatics"},{"key":"10.1016\/j.actatropica.2018.02.021_bib0080","doi-asserted-by":"crossref","first-page":"192","DOI":"10.4269\/ajtmh.1986.35.192","article-title":"Ecological study of rocky mountain spotted fever in Costa Rica","volume":"35","author":"Fuentes","year":"1986","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0085","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.3201\/eid0911.030193","article-title":"Fatal spotted fever rickettsiosis, minas gerais, Brazil","volume":"9","author":"Galvao","year":"2003","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0090","first-page":"432","article-title":"In silico identification of putative drug targets in Klebsiella pneumonia MGH78578","volume":"10","author":"Georrge","year":"2011","journal-title":"Indian J. Biotechnol."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0095","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1093\/infdis\/150.4.480","article-title":"Rocky Mountain spotted fever: clinical, laboratory and epidemiological features of 262 cases","volume":"150","author":"Helmick","year":"1984","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0100","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/381272a0","article-title":"Errors in protein structures","volume":"381","author":"Hooft","year":"1996","journal-title":"Nature"},{"key":"10.1016\/j.actatropica.2018.02.021_bib0105","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S0168-9525(97)01255-9","article-title":"Differential genome display","volume":"13","author":"Huynen","year":"1997","journal-title":"Trends Genet."},{"issue":"1","key":"10.1016\/j.actatropica.2018.02.021_bib0110","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0014-5793(98)00276-2","article-title":"Differential genome analysis applied to the species-specific features of Helicobacter pylori","volume":"426","author":"Huynen","year":"1998","journal-title":"FEBS Lett."},{"issue":"1","key":"10.1016\/j.actatropica.2018.02.021_bib0115","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0120","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1007\/s00709-010-0255-0","article-title":"In silico prediction of drug targets in Vibrio cholerae","volume":"248","author":"Katara","year":"2012","journal-title":"Protoplasma"},{"key":"10.1016\/j.actatropica.2018.02.021_bib0125","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s12010-013-0372-2","article-title":"Identification of druggable targets for acinetobacter baumannii via subtractive genomics and plausible inhibitors for MurA and MurB","volume":"171","author":"Kaur","year":"2013","journal-title":"Appl. Biochem. Biotechnol."},{"issue":"March (6364)","key":"10.1016\/j.actatropica.2018.02.021_bib0130","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/356083a0","article-title":"Assessment of protein models with three-dimensional profiles","volume":"356","author":"L\u00fcthy","year":"1992","journal-title":"Nature"},{"key":"10.1016\/j.actatropica.2018.02.021_bib0135","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK \u2013 a program to check the stereochemical quality of protein structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"J. App. Cryst."},{"issue":"8","key":"10.1016\/j.actatropica.2018.02.021_bib0140","doi-asserted-by":"crossref","first-page":"4706","DOI":"10.1128\/IAI.68.8.4706-4713.2000","article-title":"Ultrastructure of Rickettsia rickettsii actin tails and localization of cytoskeletal proteins","volume":"68","author":"Levi","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0145","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1093\/bioinformatics\/btl158","article-title":"Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences","volume":"22","author":"Li","year":"2006","journal-title":"Bioinformatics"},{"issue":"1","key":"10.1016\/j.actatropica.2018.02.021_bib0150","first-page":"174","article-title":"Putative drug targets in thypus causing Rickettsia prowazekii str: madrid E: An in silico approach","volume":"4","author":"MaliPatil","year":"2013","journal-title":"Int. J. Res. Pharm. Biomed. Sci."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0155","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1146\/annurev.mi.40.100186.001443","article-title":"Natural history of Rickettsia rickettsii","volume":"40","author":"McDade","year":"1986","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0160","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1126\/science.3099387","article-title":"Cloned gene of Rickettsia rickettsii surface antigen: candidate vaccine for Rocky Mountain spotted fever","volume":"235","author":"McDonald","year":"1987","journal-title":"Science"},{"issue":"6","key":"10.1016\/j.actatropica.2018.02.021_bib0165","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1038\/nrg1086","article-title":"Genetic strategies for antibacterial drug discovery","volume":"4","author":"Miesel","year":"2003","journal-title":"Nat. Rev. Genet."},{"issue":"2","key":"10.1016\/j.actatropica.2018.02.021_bib0170","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1093\/nar\/gkm321","article-title":"KAAS an automatic genome annotation and pathway reconstruction server","volume":"35","author":"Moriya","year":"2007","journal-title":"Nucleic Acids Res."},{"issue":"16","key":"10.1016\/j.actatropica.2018.02.021_bib0175","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1002\/jcc.21256","article-title":"Autodock4 and AutoDockTools4: automated docking with selective receptor flexiblity","volume":"2009","author":"Morris","year":"2009","journal-title":"J. Comput. Chem."},{"issue":"2","key":"10.1016\/j.actatropica.2018.02.021_bib0180","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.65.2.773-778.1999","article-title":"Lethal effect of Rickettsia rickettsii on its tick vector (Dermacentor andersoni)","volume":"65","author":"Niebylski","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0185","doi-asserted-by":"crossref","first-page":"941","DOI":"10.4269\/ajtmh.1971.20.941","article-title":"Rickettsioses of central america","volume":"20","author":"Peacock","year":"1971","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"November (1)","key":"10.1016\/j.actatropica.2018.02.021_bib0190","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1006\/jmbi.1996.0628","article-title":"Deviations from standard atomic volumes as a quality measure for protein crystal structures","volume":"264","author":"Pontius","year":"1996","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0195","doi-asserted-by":"crossref","first-page":"350","DOI":"10.4269\/ajtmh.1999.61.350","article-title":"Evidence of rickettsial spotted fever and ehrlichial infections in a subtropical territory of Jujuy, Argentina","volume":"61","author":"Ripoll","year":"1999","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0200","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mimet.2014.11.016","article-title":"In silico comparative genomics analysis of Plasmodium falciparum for the identification of putative essential genes and therapeutic candidates","volume":"109","author":"Rout","year":"2015","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.actatropica.2018.02.021_bib0205","doi-asserted-by":"crossref","first-page":"355","DOI":"10.3233\/ISB-00138","article-title":"A novel genomics approach for the identification of drug targets in pathogens, with special reference to Pseudomonas aeruginosa","volume":"4","author":"Sakharkar","year":"2004","journal-title":"In Silico Biol."},{"issue":"3","key":"10.1016\/j.actatropica.2018.02.021_bib0210","first-page":"1","article-title":"Identification and characterization of potential therapeutic candidates in emerging human pathogen Mycobacterium abscessus, a novel hierarchical in silico approach","volume":"8","author":"Shanmugham","year":"2013","journal-title":"India PLoS ONE"},{"issue":"07","key":"10.1016\/j.actatropica.2018.02.021_bib0215","first-page":"017","article-title":"Subtractive genome analysis for in silico identification and characterization of novel drug targets in C. trachomatis strain D\/UW-3\/Cx","volume":"4","author":"Shoukat","year":"2012","journal-title":"Int. J. Curr. Res."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0220","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1021\/ci200193z","article-title":"AADS \u2013 an automated active site identification, docking and scoring protocol for protein targets based on physico-chemical descriptors","volume":"51","author":"Singh","year":"2011","journal-title":"J. Chem. Inf. Modeling"},{"key":"10.1016\/j.actatropica.2018.02.021_bib0225","first-page":"388","article-title":"The rickettsial plaque: evidence for direct cytopathic effect of Rickettsia rickettsii","volume":"43","author":"Walker","year":"1980","journal-title":"Lab. Invest."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0230","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1128\/iai.37.1.301-306.1982","article-title":"Human endothelial cell culture plaques, induced by Rickettsia rickettsii","volume":"37","author":"Walker","year":"1982","journal-title":"Infect. Immun."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0235","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1093\/clinids\/20.5.1111","article-title":"Rocky Mountain spotted fever: a seasonal alert","volume":"20","author":"Walker","year":"1995","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0240","first-page":"688","article-title":"Rickettsia","volume":"Vol. I","author":"Weiss","year":"1984"},{"issue":"10","key":"10.1016\/j.actatropica.2018.02.021_bib0245","doi-asserted-by":"crossref","first-page":"3375","DOI":"10.1093\/nar\/gkm251","article-title":"LOMETS: A local meta-threading-server for protein structure prediction","volume":"35","author":"Wu","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0250","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1002\/prot.21018","article-title":"Prediction of protein subcellular localization","volume":"64","author":"Yu","year":"2006","journal-title":"Proteins"},{"key":"10.1016\/j.actatropica.2018.02.021_bib0255","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1093\/bioinformatics\/btq249","article-title":"PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes","volume":"26","author":"Yu","year":"2010","journal-title":"Bioinformatics"},{"issue":"April (4)","key":"10.1016\/j.actatropica.2018.02.021_bib0260","article-title":"Fatal human infection with Rickettsia rickettsii, Yucatan, Mexico","volume":"12","author":"Zavala-Castro","year":"2006","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.actatropica.2018.02.021_bib0265","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1093\/nar\/gkn858","article-title":"DEG 5.0: a database of essential genes in both prokaryotes and eukaryotes","volume":"37","author":"Zhang","year":"2009","journal-title":"Nucleic Acids Res."}],"container-title":["Acta Tropica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X17313475?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0001706X17313475?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:12:16Z","timestamp":1757549536000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0001706X17313475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6]]},"references-count":53,"alternative-id":["S0001706X17313475"],"URL":"https:\/\/doi.org\/10.1016\/j.actatropica.2018.02.021","relation":{},"ISSN":["0001-706X"],"issn-type":[{"value":"0001-706X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparative genomic analysis of Rickettsia rickettsii for identification of drug and vaccine targets: tolC as a proposed candidate for case study","name":"articletitle","label":"Article Title"},{"value":"Acta Tropica","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actatropica.2018.02.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}