{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:18:50Z","timestamp":1792804730646,"version":"4.5.0"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2016,8,16]],"date-time":"2016-08-16T00:00:00Z","timestamp":1471305600000},"content-version":"am","delay-in-days":350,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DMR 0955873"],"award-info":[{"award-number":["DMR 0955873"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DMR 1410341"],"award-info":[{"award-number":["DMR 1410341"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Biomaterialia"],"published-print":{"date-parts":[[2015,9]]},"DOI":"10.1016\/j.actbio.2015.06.028","type":"journal-article","created":{"date-parts":[[2015,6,24]],"date-time":"2015-06-24T17:24:51Z","timestamp":1435166691000},"page":"220-227","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":86,"special_numbering":"C","title":["Designing a fibrotic microenvironment to investigate changes in human liver sinusoidal endothelial cell function"],"prefix":"10.1016","volume":"24","author":[{"given":"Andrew J.","family":"Ford","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaurav","family":"Jain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Padmavathy","family":"Rajagopalan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.actbio.2015.06.028_b0005","first-page":"149","article-title":"Noninvasive biochemical markers of liver fibrosis","volume":"15","author":"Grigorescu","year":"2006","journal-title":"J. Gastrointest. Liver Dis."},{"key":"10.1016\/j.actbio.2015.06.028_b0010","series-title":"The Liver: Biology and Pathobiology","author":"Arias","year":"2001"},{"key":"10.1016\/j.actbio.2015.06.028_b0015","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1172\/JCI24282","article-title":"Liver fibrosis","volume":"115","author":"Bataller","year":"2005","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.actbio.2015.06.028_b0020","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/BF01724682","article-title":"Connective tissue components of the normal and fibrotic liver. I. Structure, local distribution and metabolism of connective tissue components in the normal liver and changes in chronic liver diseases","volume":"59","author":"Rauterberg","year":"1981","journal-title":"Klin. Wochenschr."},{"key":"10.1016\/j.actbio.2015.06.028_b0025","doi-asserted-by":"crossref","first-page":"G1147","DOI":"10.1152\/ajpgi.00032.2007","article-title":"Increased stiffness of the rat liver precedes matrix deposition: implications for fibrosis","volume":"293","author":"Georges","year":"2007","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"10.1016\/j.actbio.2015.06.028_b0030","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1002\/hep.23312","article-title":"Diagnosis of fibrosis and cirrhosis using liver stiffness measurement in nonalcoholic fatty liver disease","volume":"51","author":"Wong","year":"2010","journal-title":"Hepatology"},{"key":"10.1016\/j.actbio.2015.06.028_b0035","doi-asserted-by":"crossref","first-page":"49","DOI":"10.2147\/HMER.S7394","article-title":"Liver stiffness: a novel parameter for the diagnosis of liver disease","volume":"2","author":"Mueller","year":"2010","journal-title":"Hepatic Med.: Evid. Res."},{"key":"10.1016\/j.actbio.2015.06.028_b0040","series-title":"Endothelial Cells of the Hepatic Sinusoids: A Review. Liver Diseases and Hepatic Sinusoidal Cells","author":"Wisse","year":"1999"},{"key":"10.1016\/j.actbio.2015.06.028_b0045","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1476-5926-1-1","article-title":"Structural and functional aspects of liver sinusoidal endothelial cell fenestrae: a review","volume":"1","author":"Braet","year":"2002","journal-title":"Comp. Hepatol."},{"issue":"Suppl. 1","key":"10.1016\/j.actbio.2015.06.028_b0050","doi-asserted-by":"crossref","first-page":"S22","DOI":"10.1186\/1476-5926-2-S1-S22","article-title":"Clearance function of scavenger endothelial cells","volume":"3","author":"Smedsrod","year":"2004","journal-title":"Comp. Hepatol."},{"key":"10.1016\/j.actbio.2015.06.028_b0055","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1136\/gut.35.11.1509","article-title":"Cell biology of liver endothelial and Kupffer cells","volume":"35","author":"Smedsrod","year":"1994","journal-title":"Gut"},{"key":"10.1016\/j.actbio.2015.06.028_b0060","first-page":"G1187","article-title":"Rat liver endothelial cells isolated by anti-CD31 immunomagnetic separation lack fenestrae and sieve plates","volume":"291","author":"DeLeve","year":"2006","journal-title":"Am. J. Physiol.-Gastr. L."},{"key":"10.1016\/j.actbio.2015.06.028_b0065","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1016\/S0002-9440(10)63487-6","article-title":"Capillarization of hepatic sinusoid by liver endothelial cell-reactive autoantibodies in patients with cirrhosis and chronic hepatitis","volume":"163","author":"Xu","year":"2003","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.actbio.2015.06.028_b0070","doi-asserted-by":"crossref","first-page":"238","DOI":"10.3748\/wjg.v10.i2.238","article-title":"Dynamic changes of capillarization and peri-sinusoid fibrosis in alcoholic liver diseases","volume":"10","author":"Xu","year":"2004","journal-title":"World J. Gastroenterol."},{"key":"10.1016\/j.actbio.2015.06.028_b0075","doi-asserted-by":"crossref","DOI":"10.1053\/j.gastro.2011.12.017","article-title":"Role of differentiation of liver sinusoidal endothelial cells in progression and regression of hepatic fibrosis in rats","volume":"142","author":"Xie","year":"2012","journal-title":"Gastroenterology"},{"key":"10.1016\/j.actbio.2015.06.028_b0080","doi-asserted-by":"crossref","first-page":"993","DOI":"10.2353\/ajpath.2008.070720","article-title":"Prevention of hepatic fibrosis in a murine model of metabolic syndrome with nonalcoholic steatohepatitis","volume":"173","author":"DeLeve","year":"2008","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.actbio.2015.06.028_b0085","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1002\/hep.22193","article-title":"The role of matrix stiffness in regulating cell behavior","volume":"47","author":"Wells","year":"2008","journal-title":"Hepatology"},{"key":"10.1016\/j.actbio.2015.06.028_b0090","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1089\/ten.2006.0437","article-title":"Maintenance of hepatic sinusoidal endothelial cell phenotype in vitro using organ-specific extracellular matrix scaffolds","volume":"13","author":"Sellaro","year":"2007","journal-title":"Tissue Eng."},{"key":"10.1016\/j.actbio.2015.06.028_b0095","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1002\/hep.23085","article-title":"Microenvironmental regulation of the sinusoidal endothelial cell phenotype in vitro","volume":"50","author":"March","year":"2009","journal-title":"Hepatology"},{"key":"10.1016\/j.actbio.2015.06.028_b0100","first-page":"46","article-title":"Extracellular matrix rigidity controls podosome induction in microvascular endothelial cells","volume":"105","author":"Juin","year":"2013","journal-title":"Biol. Cell (under the auspices of the European Cell Biology Organization)"},{"key":"10.1016\/j.actbio.2015.06.028_b0105","first-page":"G757","article-title":"Rat liver sinusoidal endothelial cell phenotype is maintained by paracrine and autocrine regulation","volume":"287","author":"DeLeve","year":"2004","journal-title":"Am. J. Physiol.-Gastr. L."},{"key":"10.1016\/j.actbio.2015.06.028_b0110","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1016\/S0168-8278(00)80239-1","article-title":"Hepatocyte-supported serum-free culture of rat liver sinusoidal endothelial cells","volume":"32","author":"Krause","year":"2000","journal-title":"J. Hepatol."},{"key":"10.1016\/j.actbio.2015.06.028_b0115","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/0168-8278(95)80204-5","article-title":"Experimental biliary fibrosis correlates with increased numbers of fat-storing and Kupffer cells, and portal endotoxemia","volume":"23","author":"Grinko","year":"1995","journal-title":"J. Hepatol."},{"key":"10.1016\/j.actbio.2015.06.028_b0120","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1189\/jlb.0113051","article-title":"Kupffer cells potentiate liver sinusoidal endothelial cell injury in sepsis by ligating programmed cell death ligand-1","volume":"94","author":"Hutchins","year":"2013","journal-title":"J. Leukocyte Biol."},{"key":"10.1016\/j.actbio.2015.06.028_b0125","doi-asserted-by":"crossref","first-page":"2317","DOI":"10.1021\/am100346k","article-title":"Cell migration at the interface of a dual chemical\u2013mechanical gradient","volume":"2","author":"Hale","year":"2010","journal-title":"ACS App. Mater. Interfaces"},{"key":"10.1016\/j.actbio.2015.06.028_b0130","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1115\/1.2795998","article-title":"The fibroblast-populated collagen microsphere assay of cell traction force","volume":"117","author":"Barocas","year":"1995","journal-title":"J. Biomech. Eng."},{"key":"10.1016\/j.actbio.2015.06.028_b0135","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0022-5320(70)90150-4","article-title":"An electron microscopic study of the fenestrated endothelial lining of rat liver sinusoids","volume":"31","author":"Wisse","year":"1970","journal-title":"J. Ultrastruct. Res."},{"key":"10.1016\/j.actbio.2015.06.028_b0140","doi-asserted-by":"crossref","first-page":"G375","DOI":"10.1152\/ajpgi.00288.2011","article-title":"Lipids promote survival, proliferation, and maintenance of differentiation of rat liver sinusoidal endothelial cells in vitro","volume":"302","author":"Hang","year":"2012","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"10.1016\/j.actbio.2015.06.028_b0145","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1083\/jcb.114.5.1059","article-title":"Molecular and cellular properties of PECAM-1 (endoCAM\/CD31): a novel vascular cell\u2013cell adhesion molecule","volume":"114","author":"Albelda","year":"1991","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.actbio.2015.06.028_b0150","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/S0168-8278(05)80072-8","article-title":"The cell adhesion molecules of hepatic sinusoidal endothelial cells","volume":"20","author":"Scoazec","year":"1994","journal-title":"J. Hepatol."},{"key":"10.1016\/j.actbio.2015.06.028_b0155","doi-asserted-by":"crossref","first-page":"2564","DOI":"10.1096\/fj.06-7473com","article-title":"Rat liver sinusoidal endothelial cells survive without exogenous VEGF in 3D perfused co-cultures with hepatocytes","volume":"21","author":"Hwa","year":"2007","journal-title":"FASEB J."},{"key":"10.1016\/j.actbio.2015.06.028_b0160","first-page":"738","article-title":"Expression of cell\u2013cell and cell\u2013matrix adhesion proteins by sinusoidal endothelial cells in the normal and cirrhotic human liver","volume":"143","author":"Couvelard","year":"1993","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.actbio.2015.06.028_b0165","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1016\/0046-8177(95)90274-0","article-title":"Focal nodular hyperplasia of the liver: composition of the extracellular matrix and expression of cell\u2013cell and cell\u2013matrix adhesion molecules","volume":"26","author":"Scoazec","year":"1995","journal-title":"Hum. Pathol."},{"key":"10.1016\/j.actbio.2015.06.028_b0170","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1053\/gast.2001.20947","article-title":"Immunology of the healthy liver: old questions and new insights","volume":"120","author":"Mehal","year":"2001","journal-title":"Gastroenterology"},{"key":"10.1016\/j.actbio.2015.06.028_b0175","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/j.jhep.2010.04.021","article-title":"Activation of human liver sinusoidal endothelial cell by human platelets induces hepatocyte proliferation","volume":"53","author":"Kawasaki","year":"2010","journal-title":"J. Hepatol."},{"key":"10.1016\/j.actbio.2015.06.028_b0180","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1002\/hep.22351","article-title":"Sinusoidal endothelial cells prevent rat stellate cell activation and promote reversion to quiescence","volume":"48","author":"Deleve","year":"2008","journal-title":"Hepatology"},{"key":"10.1016\/j.actbio.2015.06.028_b0185","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1472-6793-8-9","article-title":"Decrease of PECAM-1-gene-expression induced by proinflammatory cytokines IFN-gamma and IFN-alpha is reversed by TGF-beta in sinusoidal endothelial cells and hepatic mononuclear phagocytes","volume":"8","author":"Neubauer","year":"2008","journal-title":"BMC Physiol."},{"key":"10.1016\/j.actbio.2015.06.028_b0190","doi-asserted-by":"crossref","first-page":"5429","DOI":"10.3748\/wjg.v12.i34.5429","article-title":"Human hepatic sinusoidal endothelial cells can be distinguished by expression of phenotypic markers related to their specialised functions in vivo","volume":"12","author":"Lalor","year":"2006","journal-title":"World J. Gastroenterol.: WJG"},{"key":"10.1016\/j.actbio.2015.06.028_b0195","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/labinvest.3700114","article-title":"Rho modulates hepatic sinusoidal endothelial fenestrae via regulation of the actin cytoskeleton in rat endothelial cells","volume":"84","author":"Yokomori","year":"2004","journal-title":"Lab. Invest. J. Tech. Meth. Pathol."},{"key":"10.1016\/j.actbio.2015.06.028_b0200","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1242\/jcs.044487","article-title":"Looking beyond death: a morphogenetic role for the TNF signalling pathway","volume":"122","author":"Mathew","year":"2009","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.actbio.2015.06.028_b0205","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1023\/A:1021304227369","article-title":"Expression of cell adhesion molecules, their ligands and tumour necrosis factor alpha in the liver of patients with metastatic gastrointestinal carcinomas","volume":"34","author":"Gulubova","year":"2002","journal-title":"Histochem. J."},{"key":"10.1016\/j.actbio.2015.06.028_b0210","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1007\/s005950200130","article-title":"Cellular localization of tumor necrosis factor-alpha mRNA and interleukin-6 mRNA in the rat liver after hemorrhagic shock","volume":"32","author":"Yamashita","year":"2002","journal-title":"Surg. Today"},{"key":"10.1016\/j.actbio.2015.06.028_b0215","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1016\/S0016-5085(99)70408-7","article-title":"Impaired endothelial nitric oxide synthase activity associated with enhanced caveolin binding in experimental cirrhosis in the rat","volume":"117","author":"Shah","year":"1999","journal-title":"Gastroenterology"},{"key":"10.1016\/j.actbio.2015.06.028_b0220","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/S0016-5085(98)70487-1","article-title":"Reduced nitric oxide production by endothelial cells in cirrhotic rat liver: endothelial dysfunction in portal hypertension","volume":"114","author":"Rockey","year":"1998","journal-title":"Gastroenterology"},{"key":"10.1016\/j.actbio.2015.06.028_b0225","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1471-2121-3-7","article-title":"The new anti-actin agent dihydrohalichondramide reveals fenestrae-forming centers in hepatic endothelial cells","volume":"3","author":"Braet","year":"2002","journal-title":"BMC Cell Biol."},{"key":"10.1016\/j.actbio.2015.06.028_b0230","first-page":"143","article-title":"Rac1, caveolin-1 and vascular endothelial growth factor -mediated liver sinusoidal endothelial cell angiogenesis","volume":"29","author":"Braet","year":"2009","journal-title":"Liver Int. Off. J. Int. Assoc. Study Liver"},{"key":"10.1016\/j.actbio.2015.06.028_b0235","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1002\/(SICI)1097-4652(199807)176:1<150::AID-JCP17>3.0.CO;2-B","article-title":"Regulation of TNF-alpha-induced reorganization of the actin cytoskeleton and cell\u2013cell junctions by Rho, Rac, and Cdc42 in human endothelial cells","volume":"176","author":"Wojciak-Stothard","year":"1998","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.actbio.2015.06.028_b0240","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1007\/s00249-012-0851-3","article-title":"Endothelial cells undergo morphological, biomechanical, and dynamic changes in response to tumor necrosis factor-alpha","volume":"41","author":"Stroka","year":"2012","journal-title":"Eur. Biophys. J.: EBJ"},{"key":"10.1016\/j.actbio.2015.06.028_b0245","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1007\/BF03402127","article-title":"TNF-alpha induces actin cytoskeleton reorganization in glomerular epithelial cells involving tyrosine phosphorylation of paxillin and focal adhesion kinase","volume":"5","author":"Koukouritaki","year":"1999","journal-title":"Mol. Med."},{"key":"10.1016\/j.actbio.2015.06.028_b0250","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1242\/jcs.067009","article-title":"Mechanical signaling through the cytoskeleton regulates cell proliferation by coordinated focal adhesion and Rho GTPase signaling","volume":"124","author":"Provenzano","year":"2011","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.actbio.2015.06.028_b0255","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/S0006-3495(04)74140-5","article-title":"Substrate compliance versus ligand density in cell on gel responses","volume":"86","author":"Engler","year":"2004","journal-title":"Biophys. J."}],"container-title":["Acta Biomaterialia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1742706115300015?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1742706115300015?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T16:46:30Z","timestamp":1761669990000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1742706115300015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":51,"alternative-id":["S1742706115300015"],"URL":"https:\/\/doi.org\/10.1016\/j.actbio.2015.06.028","relation":{},"ISSN":["1742-7061"],"issn-type":[{"value":"1742-7061","type":"print"}],"subject":[],"published":{"date-parts":[[2015,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Designing a fibrotic microenvironment to investigate changes in human liver sinusoidal endothelial cell function","name":"articletitle","label":"Article Title"},{"value":"Acta Biomaterialia","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actbio.2015.06.028","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}