{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:39:33Z","timestamp":1792067973712,"version":"4.4.0"},"reference-count":93,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100013105","name":"Shanghai Rising-Star Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013105","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Biomaterialia"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1016\/j.actbio.2020.09.041","type":"journal-article","created":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T19:47:11Z","timestamp":1601408831000},"page":"400-417","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":175,"special_numbering":"C","title":["Biodegradable Zn\u2013Cu alloys show antibacterial activity against MRSA bone infection by inhibiting pathogen adhesion and biofilm formation"],"prefix":"10.1016","volume":"117","author":[{"given":"Xinhua","family":"Qu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0495-1554","authenticated-orcid":false,"given":"Hongtao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifeng","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7402-9979","authenticated-orcid":false,"given":"Yufeng","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerong","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.actbio.2020.09.041_bib0001","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1007\/s00402-013-1785-7","article-title":"Osteomyelitis","volume":"133","author":"Hogan","year":"2013","journal-title":"Arch. Orthop. Trauma Surg."},{"issue":"1","key":"10.1016\/j.actbio.2020.09.041_bib0002","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1186\/s12891-017-1506-4","article-title":"Incidence of infection following internal fixation of open and closed tibia fractures in India (INFINITI): a multi-centre observational cohort study","volume":"18","author":"Doshi","year":"2017","journal-title":"BMC Musculoskelet. Disord."},{"issue":"1","key":"10.1016\/j.actbio.2020.09.041_bib0003","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1186\/s13018-018-1003-y","article-title":"Incidence and predictors of surgical site infection after ORIF in calcaneus fractures, a retrospective cohort study","volume":"13","author":"Wang","year":"2018","journal-title":"J. Orthop. Surg. Res."},{"issue":"4 Suppl","key":"10.1016\/j.actbio.2020.09.041_bib0004","doi-asserted-by":"crossref","first-page":"31S","DOI":"10.1177\/2192568217752130","article-title":"Surgical site infections in spine surgery: preoperative prevention strategies to minimize risk","volume":"8","author":"Spina","year":"2018","journal-title":"Glob. Spine J."},{"issue":"3","key":"10.1016\/j.actbio.2020.09.041_bib0005","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.jinf.2019.07.003","article-title":"Timing of implant-removal in late acute periprosthetic joint infection: a multicenter observational study","volume":"79","author":"Wouthuyzen-Bakker","year":"2019","journal-title":"J. Infect."},{"issue":"2","key":"10.1016\/j.actbio.2020.09.041_bib0006","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1053\/jhin.2001.1052","article-title":"Implant infections: a haven for opportunistic bacteria","volume":"49","author":"Schierholz","year":"2001","journal-title":"J. Hosp. Infect."},{"issue":"9431","key":"10.1016\/j.actbio.2020.09.041_bib0007","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0140-6736(04)16727-5","article-title":"Osteomyelitis","volume":"364","author":"Lew","year":"2004","journal-title":"Lancet"},{"issue":"10","key":"10.1016\/j.actbio.2020.09.041_bib0008","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1172\/JCI200320365","article-title":"The application of biofilm science to the study and control of chronic bacterial infections","volume":"112","author":"Costerton","year":"2003","journal-title":"J. Clin. Investig."},{"key":"10.1016\/j.actbio.2020.09.041_bib0009","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1146\/annurev-biochem-060614-034516","article-title":"Mechanisms of methicillin resistance in staphylococcus aureus","volume":"84","author":"Peacock","year":"2015","journal-title":"Ann. Rev. Biochem."},{"key":"10.1016\/j.actbio.2020.09.041_bib0010","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.biomaterials.2016.08.031","article-title":"Biodegradable Mg-Cu alloy implants with antibacterial activity for the treatment of osteomyelitis: In vitro and in vivo evaluations","volume":"106","author":"Li","year":"2016","journal-title":"Biomaterials"},{"issue":"12","key":"10.1016\/j.actbio.2020.09.041_bib0011","doi-asserted-by":"crossref","first-page":"7586","DOI":"10.1128\/AAC.03936-14","article-title":"Antibacterial properties of magnesium in vitro and in an in vivo model of implant-associated methicillin-resistant staphylococcus aureus infection","volume":"58","author":"Li","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.actbio.2020.09.041_bib0012","doi-asserted-by":"crossref","first-page":"27374","DOI":"10.1038\/srep27374","article-title":"Biodegradable Mg-Cu alloys with enhanced osteogenesis, angiogenesis, and long-lasting antibacterial effects","volume":"6","author":"Liu","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.actbio.2020.09.041_bib0013","doi-asserted-by":"crossref","DOI":"10.1016\/j.actbio.2019.05.069","article-title":"Corrosion resistance and antibacterial activity of zinc-loaded montmorillonite coatings on biodegradable magnesium alloy AZ31","author":"Zou","year":"2019","journal-title":"Acta biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0014","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.colsurfb.2016.02.004","article-title":"Mussel-inspired functionalization of PEO\/PCL composite coating on a biodegradable AZ31 magnesium alloy","volume":"141","author":"Tian","year":"2016","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.actbio.2020.09.041_bib0015","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1016\/j.surfcoat.2017.06.015","article-title":"Electrodeposition of TiO 2 layer-by-layer assembled composite coating and silane treatment on Mg alloy for corrosion resistance","volume":"324","author":"Cui","year":"2017","journal-title":"Surf. Coat. Technol."},{"issue":"12","key":"10.1016\/j.actbio.2020.09.041_bib0016","doi-asserted-by":"crossref","first-page":"4028","DOI":"10.1016\/S1003-6326(15)64052-3","article-title":"vitro corrosion and antibacterial properties of layer-by-layer assembled GS\/PSS coating on AZ31 magnesium alloys","volume":"25","author":"Zeng","year":"2015","journal-title":"Trans. Nonferrous Metals Soc. China"},{"key":"10.1016\/j.actbio.2020.09.041_bib0017","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/j.corsci.2016.05.039","article-title":"Comparative corrosion behavior of Zn with Fe and Mg in the course of immersion degradation in phosphate buffered saline","volume":"111","author":"Chen","year":"2016","journal-title":"Corros. Sci."},{"issue":"18","key":"10.1016\/j.actbio.2020.09.041_bib0018","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1002\/adma.201300226","article-title":"Zinc exhibits ideal physiological corrosion behavior for bioabsorbable stents","volume":"25","author":"Bowen","year":"2013","journal-title":"Adv. Mater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0019","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.actbio.2018.03.007","article-title":"In vitro and in vivo studies on zinc-hydroxyapatite composites as novel biodegradable metal matrix composite for orthopedic applications","volume":"71","author":"Yang","year":"2018","journal-title":"Acta Biomater."},{"issue":"2","key":"10.1016\/j.actbio.2020.09.041_bib0020","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1021\/acsbiomaterials.8b01137","article-title":"Enhanced osseointegration of Zn-Mg composites by tuning the release of Zn ions with sacrificial Mg-rich anode design","volume":"5","author":"Yang","year":"2018","journal-title":"ACS Biomater. Sci. Eng."},{"key":"10.1016\/j.actbio.2020.09.041_bib0021","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.biomaterials.2017.08.022","article-title":"Evolution of the degradation mechanism of pure zinc stent in the one-year study of rabbit abdominal aorta model","volume":"145","author":"Yang","year":"2017","journal-title":"Biomaterials"},{"issue":"1","key":"10.1016\/j.actbio.2020.09.041_bib0022","first-page":"1","article-title":"Alloying design of biodegradable zinc as promising bone implants for load-bearing applications","volume":"11","author":"Yang","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.actbio.2020.09.041_bib0023","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.jmbbm.2017.05.013","article-title":"Potential biodegradable Zn-Cu binary alloys developed for cardiovascular implant applications","volume":"72","author":"Tang","year":"2017","journal-title":"J. Mech. Behav. Biomed. Mater."},{"issue":"5","key":"10.1016\/j.actbio.2020.09.041_bib0024","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/adhm.201500712","article-title":"Toward a molecular understanding of the antibacterial mechanism of copper\u2010bearing titanium alloys against staphylococcus aureus","volume":"5","author":"Li","year":"2016","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0025","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/j.msec.2015.12.062","article-title":"Antibacterial titanium surfaces for medical implants","volume":"61","author":"Ferraris","year":"2016","journal-title":"Mater. Sci. Eng. C-Mater. Biol. Appl."},{"issue":"1-2","key":"10.1016\/j.actbio.2020.09.041_bib0026","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0010-8545(02)00086-3","article-title":"Copper complexes of non-steroidal anti-inflammatory drugs: an opportunity yet to be realized","volume":"232","author":"Weder","year":"2002","journal-title":"Coord. Chem. Rev."},{"key":"10.1016\/j.actbio.2020.09.041_bib0027","doi-asserted-by":"crossref","first-page":"29985","DOI":"10.1038\/srep29985","article-title":"Antibacterial effect of copper-bearing titanium alloy (Ti-Cu) against streptococcus mutans and porphyromonas gingivalis","volume":"6","author":"Liu","year":"2016","journal-title":"Sci. Rep."},{"issue":"8","key":"10.1016\/j.actbio.2020.09.041_bib0028","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1016\/j.dental.2018.04.007","article-title":"In vitro and in vivo studies of anti-bacterial copper-bearing titanium alloy for dental application","volume":"34","author":"Liu","year":"2018","journal-title":"Dent. Mater.: off. Publ. Acad. Dent. Mater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0029","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1016\/j.msec.2016.07.050","article-title":"Inhibition of Staphylococcus aureus biofilm by a copper-bearing 317L-Cu stainless steel and its corrosion resistance","volume":"69","author":"Sun","year":"2016","journal-title":"Mater. Sci. Eng.. C, Mater. Biol. Appl."},{"key":"10.1016\/j.actbio.2020.09.041_bib0030","doi-asserted-by":"crossref","first-page":"29244","DOI":"10.1038\/srep29244","article-title":"An investigation of the antibacterial ability and cytotoxicity of a novel cu-bearing 317L stainless steel","volume":"6","author":"Sun","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.actbio.2020.09.041_bib0031","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.colsurfb.2012.12.025","article-title":"Antibacterial activity against Porphyromonas gingivalis and biological characteristics of antibacterial stainless steel","volume":"105","author":"Zhang","year":"2013","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.actbio.2020.09.041_bib0032","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.msec.2016.11.126","article-title":"Preliminary study on the corrosion resistance, antibacterial activity and cytotoxicity of selective-laser-melted Ti6Al4V-xCu alloys","volume":"72","author":"Guo","year":"2017","journal-title":"Mater. Sci. Eng. C, Mater. Biol. Appl."},{"key":"10.1016\/j.actbio.2020.09.041_bib0033","doi-asserted-by":"crossref","first-page":"29985","DOI":"10.1038\/srep29985","article-title":"Antibacterial effect of copper-bearing titanium alloy (Ti-Cu) against streptococcus mutans and porphyromonas gingivalis","volume":"6","author":"Liu","year":"2016","journal-title":"Sci. Rep."},{"issue":"5","key":"10.1016\/j.actbio.2020.09.041_bib0034","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s10856-016-5698-1","article-title":"In vitro study on an antibacterial Ti-5Cu alloy for medical application","volume":"27","author":"Ma","year":"2016","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"10.1016\/j.actbio.2020.09.041_bib0035","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.actbio.2019.11.031","article-title":"Degradation behavior, cytotoxicity, hemolysis, and antibacterial properties of electro-deposited Zn\u2013Cu metal foams as potential biodegradable bone implants","volume":"102","author":"Tong","year":"2020","journal-title":"Acta Biomater."},{"issue":"5","key":"10.1016\/j.actbio.2020.09.041_bib0036","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1128\/JCM.02834-13","article-title":"Adjunctive mecA PCR for routine detection of methicillin susceptibility in clinical isolates of coagulase-negative staphylococci","volume":"52","author":"Nijjar","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.actbio.2020.09.041_bib0037","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.actbio.2015.04.011","article-title":"Recommendation for modifying current cytotoxicity testing standards for biodegradable magnesium-based materials","volume":"21","author":"Wang","year":"2015","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0038","series-title":"Biological Evaluation of Medical Devices. Part 5: Tests for In Vitro Cytotoxicity","year":"2009"},{"issue":"51","key":"10.1016\/j.actbio.2020.09.041_bib0039","doi-asserted-by":"crossref","first-page":"44332","DOI":"10.1021\/acsami.7b15975","article-title":"Degradation of bioresorbable Mg-4Zn-1Sr intramedullary pins and associated biological responses in vitro and in vivo","volume":"9","author":"Cipriano","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.actbio.2020.09.041_bib0040","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.biomaterials.2015.06.031","article-title":"In vitro and in vivo studies on the degradation of high-purity Mg (99.99wt.%) screw with femoral intracondylar fractured rabbit model","volume":"64","author":"Han","year":"2015","journal-title":"Biomaterials"},{"issue":"3","key":"10.1016\/j.actbio.2020.09.041_bib0041","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1073\/pnas.1518238113","article-title":"Long-term clinical study and multiscale analysis of in vivo biodegradation mechanism of Mg alloy","volume":"113","author":"Lee","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.actbio.2020.09.041_bib0042","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.actbio.2014.09.041","article-title":"Effect of the addition of low rare earth elements (lanthanum, neodymium, cerium) on the biodegradation and biocompatibility of magnesium","volume":"11","author":"Willbold","year":"2015","journal-title":"Acta Biomater."},{"issue":"17","key":"10.1016\/j.actbio.2020.09.041_bib0043","doi-asserted-by":"crossref","first-page":"3557","DOI":"10.1016\/j.biomaterials.2004.09.049","article-title":"In vivo corrosion of four magnesium alloys and the associated bone response","volume":"26","author":"Witte","year":"2005","journal-title":"Biomaterials"},{"key":"10.1016\/j.actbio.2020.09.041_bib0044","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.biomaterials.2015.11.038","article-title":"Vascularized bone grafting fixed by biodegradable magnesium screw for treating osteonecrosis of the femoral head","volume":"81","author":"Zhao","year":"2016","journal-title":"Biomaterials"},{"key":"10.1016\/j.actbio.2020.09.041_bib0045","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.biomaterials.2016.10.017","article-title":"Current status on clinical applications of magnesium-based orthopaedic implants: a review from clinical translational perspective","volume":"112","author":"Zhao","year":"2017","journal-title":"Biomaterials"},{"issue":"6","key":"10.1016\/j.actbio.2020.09.041_bib0046","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1016\/j.actbio.2012.02.018","article-title":"In vitro and in vivo studies on a Mg-Sr binary alloy system developed as a new kind of biodegradable metal","volume":"8","author":"Gu","year":"2012","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0047","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.actbio.2016.03.041","article-title":"Intramedullary Mg2Ag nails augment callus formation during fracture healing in mice","volume":"36","author":"Jahn","year":"2016","journal-title":"Acta Biomater."},{"issue":"10","key":"10.1016\/j.actbio.2020.09.041_bib0048","doi-asserted-by":"crossref","first-page":"8548","DOI":"10.1016\/j.actbio.2012.08.028","article-title":"Long-term in vivo degradation behaviour and biocompatibility of the magnesium alloy ZEK100 for use as a biodegradable bone implant","volume":"9","author":"Dziuba","year":"2013","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0049","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.actbio.2015.07.025","article-title":"In vivo evaluation of a magnesium-based degradable intramedullary nailing system in a sheep model","volume":"25","author":"Rossig","year":"2015","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0050","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/1475-925X-12-62","article-title":"Biodegradable magnesium-based screw clinically equivalent to titanium screw in hallux valgus surgery: short term results of the first prospective, randomized, controlled clinical pilot study","volume":"12","author":"Windhagen","year":"2013","journal-title":"Biomed. Eng. Online"},{"key":"10.1016\/j.actbio.2020.09.041_bib0051","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.actbio.2015.02.010","article-title":"In vivo study of magnesium plate and screw degradation and bone fracture healing","volume":"18","author":"Chaya","year":"2015","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0052","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.actbio.2015.11.014","article-title":"An in vivo study on the metabolism and osteogenic activity of bioabsorbable Mg-1Sr alloy","volume":"29","author":"Tie","year":"2016","journal-title":"Acta Biomater."},{"issue":"5","key":"10.1016\/j.actbio.2020.09.041_bib0053","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1016\/j.actbio.2013.12.040","article-title":"Magnesium alloys as a biomaterial for degradable craniofacial screws","volume":"10","author":"Henderson","year":"2014","journal-title":"Acta Biomater"},{"issue":"3","key":"10.1016\/j.actbio.2020.09.041_bib0054","first-page":"343","article-title":"Biocompatibility of magnesium-zinc alloy in biodegradable orthopedic implants","volume":"28","author":"Chen","year":"2011","journal-title":"Int. J. Mol. Med."},{"issue":"3","key":"10.1016\/j.actbio.2020.09.041_bib0055","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1002\/jbm.a.32132","article-title":"In vivo evaluation of biodegradable magnesium alloy bone implant in the first 6 months implantation","volume":"90","author":"Zhang","year":"2009","journal-title":"J. Biomed. Mater. Res. A"},{"issue":"23","key":"10.1016\/j.actbio.2020.09.041_bib0056","doi-asserted-by":"crossref","first-page":"21525","DOI":"10.1021\/am506543a","article-title":"Enhancement of osteogenesis and biodegradation control by brushite coating on Mg-Nd-Zn-Zr alloy for mandibular bone repair","volume":"6","author":"Guan","year":"2014","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.actbio.2020.09.041_bib0057","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.jmbbm.2012.02.002","article-title":"Microstructure, mechanical properties, biocorrosion behavior, and cytotoxicity of as-extruded Mg-Nd-Zn-Zr alloy with different extrusion ratios","volume":"9","author":"Zhang","year":"2012","journal-title":"J. Mech. Behav. Biomed. Mater."},{"issue":"3","key":"10.1016\/j.actbio.2020.09.041_bib0058","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1016\/j.actbio.2010.10.031","article-title":"Biomechanical testing and degradation analysis of MgCa0.8 alloy screws: a comparative in vivo study in rabbits","volume":"7","author":"Erdmann","year":"2011","journal-title":"Acta Biomater."},{"issue":"5","key":"10.1016\/j.actbio.2020.09.041_bib0059","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1016\/j.actbio.2013.12.020","article-title":"Loss of mechanical properties in vivo and bone-implant interface strength of AZ31B magnesium alloy screws with Si-containing coating","volume":"10","author":"Tan","year":"2014","journal-title":"Acta Biomater."},{"issue":"10","key":"10.1016\/j.actbio.2020.09.041_bib0060","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1016\/j.biomaterials.2007.12.021","article-title":"The development of binary Mg-Ca alloys for use as biodegradable materials within bone","volume":"29","author":"Li","year":"2008","journal-title":"Biomaterials"},{"issue":"38","key":"10.1016\/j.actbio.2020.09.041_bib0061","doi-asserted-by":"crossref","first-page":"9863","DOI":"10.1016\/j.biomaterials.2013.08.052","article-title":"In vivo stimulation of bone formation by aluminum and oxygen plasma surface-modified magnesium implants","volume":"34","author":"Wong","year":"2013","journal-title":"Biomaterials"},{"key":"10.1016\/j.actbio.2020.09.041_bib0062","doi-asserted-by":"crossref","DOI":"10.1016\/j.actbio.2019.04.045","article-title":"A pH-sensitive self-healing coating for biodegradable magnesium implants","author":"Xiong","year":"2019","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0063","doi-asserted-by":"crossref","DOI":"10.1016\/j.actbio.2019.04.046","article-title":"Additive manufacturing of biodegradable metals: current research status and future perspectives","author":"Qin","year":"2019","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0064","article-title":"In vitro and in vivo studies of Mg-30Sc alloys with different phase structure for potential usage within bone","author":"Liu","year":"2019","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0065","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.actbio.2018.08.030","article-title":"Advances in functionalized polymer coatings on biodegradable magnesium alloys - a review","volume":"79","author":"Li","year":"2018","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0066","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.actbio.2017.08.021","article-title":"Study on the Mg-Li-Zn ternary alloy system with improved mechanical properties, good degradation performance and different responses to cells","volume":"62","author":"Liu","year":"2017","journal-title":"Acta Biomater."},{"issue":"3","key":"10.1016\/j.actbio.2020.09.041_bib0067","article-title":"Long-term clinical study and multiscale analysis of in vivo biodegradation mechanism of Mg alloy","volume":"113","author":"Lee","year":"2016","journal-title":"Proceed. Natl. Acad. Sci. United States Am."},{"key":"10.1016\/j.actbio.2020.09.041_bib0068","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.biomaterials.2016.08.031","article-title":"Biodegradable Mg-Cu alloy implants with antibacterial activity for the treatment of osteomyelitis: in vitro and in vivo evaluations","volume":"106","author":"Li","year":"2016","journal-title":"Biomaterials"},{"key":"10.1016\/j.actbio.2020.09.041_bib0069","article-title":"In vitro and in vivo studies on zinc-hydroxyapatite composites as novel biodegradable metal matrix composite for orthopedic applications","author":"Yang","year":"2018","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0070","doi-asserted-by":"crossref","unstructured":"H. Yang, X. Qu, W. Lin, D. Chen, D. Zhu, K. Dai, Y.J.A.B.S. Zheng, Engineering, enhanced osseointegration of Zn-Mg composites by tuning the release of Zn ions with sacrificial Mg rich anode design, (2018).","DOI":"10.1021\/acsbiomaterials.8b01137"},{"key":"10.1016\/j.actbio.2020.09.041_bib0071","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.matdes.2015.05.089","article-title":"Design and characterizations of novel biodegradable ternary Zn-based alloys with IIA nutrient alloying elements Mg, Ca and Sr","volume":"83","author":"Li","year":"2015","journal-title":"Mater. Des."},{"key":"10.1016\/j.actbio.2020.09.041_bib0072","doi-asserted-by":"crossref","DOI":"10.1016\/j.actbio.2020.03.009","article-title":"In vitro and in vivo studies of Zn-Mn biodegradable metals designed for orthopedic applications","author":"Jia","year":"2020","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0073","article-title":"Challenges in the use of zinc and its alloys as biodegradable metals: perspective from biomechanical compatibility","author":"Li","year":"2019","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0074","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.actbio.2020.02.017","article-title":"A biodegradable Zn-1Cu-0.1Ti alloy with antibacterial properties for orthopedic applications","volume":"106","author":"Lin","year":"2020","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2020.09.041_bib0075","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.actbio.2019.11.031","article-title":"Degradation behavior, cytotoxicity, hemolysis, and antibacterial properties of electro-deposited Zn-Cu metal foams as potential biodegradable bone implants","volume":"102","author":"Tong","year":"2020","journal-title":"Acta Biomater."},{"issue":"1","key":"10.1016\/j.actbio.2020.09.041_bib0076","article-title":"Effect of Ni and Zn elements on the microstructure and antibacterial properties of Cu coatings","volume":"142","author":"Alathel","year":"2020","journal-title":"J. Eng. Mater. Technol.Trans. Asme"},{"key":"10.1016\/j.actbio.2020.09.041_bib0077","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.jmbbm.2017.05.013","article-title":"Potential biodegradable Zn-Cu binary alloys developed for cardiovascular implant applications","volume":"72","author":"Tang","year":"2017","journal-title":"J. Mech. Behav. Biomed. Mater."},{"issue":"6","key":"10.1016\/j.actbio.2020.09.041_bib0078","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.bioorg.2009.09.001","article-title":"Phosphorylated hydroxyethylamines as novel inhibitors of the bacterial cell wall biosynthesis enzymes MurC to MurF","volume":"37","author":"Sova","year":"2009","journal-title":"Bioorg. Chem."},{"issue":"7","key":"10.1016\/j.actbio.2020.09.041_bib0079","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/s41579-018-0019-y","article-title":"Implant infections: adhesion, biofilm formation and immune evasion","volume":"16","author":"Arciola","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.actbio.2020.09.041_bib0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.phrs.2020.105067","article-title":"Antibacterial and antibiofilm effects of flufenamic acid against methicillin-resistant staphylococcus aureus","volume":"160","author":"Zhang","year":"2020","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.actbio.2020.09.041_bib0081","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.actbio.2016.03.011","article-title":"Preparation of copper-containing bioactive glass\/eggshell membrane nanocomposites for improving angiogenesis, antibacterial activity and wound healing","volume":"36","author":"Li","year":"2016","journal-title":"Acta Biomater."},{"issue":"5","key":"10.1016\/j.actbio.2020.09.041_bib0082","doi-asserted-by":"crossref","first-page":"445","DOI":"10.4161\/viru.2.5.17724","article-title":"Staphylococcus aureus biofilms: properties, regulation, and roles in human disease","volume":"2","author":"Archer","year":"2011","journal-title":"Virulence"},{"issue":"6","key":"10.1016\/j.actbio.2020.09.041_bib0083","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1099\/jmm.0.000059","article-title":"Effects of erythromycin on the phenotypic and genotypic biofilm expression in two clinical Staphylococcus capitis subspecies and a functional analysis of Ica proteins in S. capitis","volume":"64","author":"Cui","year":"2015","journal-title":"J. Med. Microbiol."},{"issue":"1","key":"10.1016\/j.actbio.2020.09.041_bib0084","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.ejcb.2009.10.005","article-title":"Structure, function and contribution of polysaccharide intercellular adhesin (PIA) to Staphylococcus epidermidis biofilm formation and pathogenesis of biomaterial-associated infections","volume":"89","author":"Rohde","year":"2010","journal-title":"Eur. J. Cell Biol."},{"issue":"5","key":"10.1016\/j.actbio.2020.09.041_bib0085","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1093\/nar\/gkm1176","article-title":"Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation in Staphylococcus epidermidis","volume":"36","author":"Jeng","year":"2008","journal-title":"Nucleic. Acids Res."},{"issue":"4","key":"10.1016\/j.actbio.2020.09.041_bib0086","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1128\/IAI.72.4.2177-2185.2004","article-title":"Role of biofilm-associated protein bap in the pathogenesis of bovine Staphylococcus aureus","volume":"72","author":"Cucarella","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1016\/j.actbio.2020.09.041_bib0087","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.3389\/fmicb.2017.01401","article-title":"Pathogenic mechanisms and host interactions in staphylococcus epidermidis device-related infection","volume":"8","author":"Sabate Bresco","year":"2017","journal-title":"Front Microbiol."},{"issue":"15","key":"10.1016\/j.actbio.2020.09.041_bib0088","doi-asserted-by":"crossref","first-page":"4767","DOI":"10.1128\/JB.00348-09","article-title":"The Staphylococcus aureus LytSR two-component regulatory system affects biofilm formation","volume":"191","author":"Sharma-Kuinkel","year":"2009","journal-title":"J. Bacteriol."},{"issue":"15","key":"10.1016\/j.actbio.2020.09.041_bib0089","doi-asserted-by":"crossref","first-page":"5486","DOI":"10.1128\/JB.187.15.5486-5492.2005","article-title":"Global regulation of gene expression by ArlRS, a two-component signal transduction regulatory system of staphylococcus aureus","volume":"187","author":"Liang","year":"2005","journal-title":"J. Bacteriol."},{"issue":"7","key":"10.1016\/j.actbio.2020.09.041_bib0090","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s10856-019-6277-z","article-title":"In vitro study on cytocompatibility and osteogenesis ability of Ti-Cu alloy","volume":"30","author":"Liu","year":"2019","journal-title":"J. Mater. Sci. Mater. Med."},{"issue":"11","key":"10.1016\/j.actbio.2020.09.041_bib0091","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1016\/j.jmst.2019.04.028","article-title":"Osteogenesis stimulation by copper-containing 316L stainless steel via activation of akt cell signaling pathway and Runx2 upregulation","volume":"35","author":"Yuan","year":"2019","journal-title":"J. Mater. Sci. Technol."},{"issue":"7","key":"10.1016\/j.actbio.2020.09.041_bib0092","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1002\/jbm.b.33318","article-title":"Osteogenic ability of Cu-bearing stainless steel","volume":"103","author":"Ren","year":"2015","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater"},{"issue":"11","key":"10.1016\/j.actbio.2020.09.041_bib0093","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1002\/adhm.201500824","article-title":"Graphene oxide-copper nanocomposite-coated porous CaP scaffold for vascularized bone regeneration via activation of hif-1alpha","volume":"5","author":"Zhang","year":"2016","journal-title":"Adv. Healthc. Mater."}],"container-title":["Acta Biomaterialia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S174270612030564X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S174270612030564X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T17:34:52Z","timestamp":1761672892000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S174270612030564X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":93,"alternative-id":["S174270612030564X"],"URL":"https:\/\/doi.org\/10.1016\/j.actbio.2020.09.041","relation":{},"ISSN":["1742-7061"],"issn-type":[{"value":"1742-7061","type":"print"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Biodegradable Zn\u2013Cu alloys show antibacterial activity against MRSA bone infection by inhibiting pathogen adhesion and biofilm formation","name":"articletitle","label":"Article Title"},{"value":"Acta Biomaterialia","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actbio.2020.09.041","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}