{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:26:14Z","timestamp":1790760374428,"version":"4.1.0"},"reference-count":84,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["XDC07040200"],"award-info":[{"award-number":["XDC07040200"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018AAA0100300"],"award-info":[{"award-number":["2018AAA0100300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["WK2480000006"],"award-info":[{"award-number":["WK2480000006"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["WK9100000001"],"award-info":[{"award-number":["WK9100000001"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["WK9110000125"],"award-info":[{"award-number":["WK9110000125"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013804","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013804","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571768"],"award-info":[{"award-number":["81571768"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072018"],"award-info":[{"award-number":["82072018"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["91859121"],"award-info":[{"award-number":["91859121"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Biomaterialia"],"published-print":{"date-parts":[[2021,10]]},"DOI":"10.1016\/j.actbio.2021.07.013","type":"journal-article","created":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T02:37:18Z","timestamp":1626143838000},"page":"228-239","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":72,"special_numbering":"C","title":["Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply"],"prefix":"10.1016","volume":"134","author":[{"given":"Kun","family":"Du","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shibo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengpan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunguang","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianzhi","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bensheng","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiping","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.actbio.2021.07.013_bib0001","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/s41575-020-0269-9","article-title":"Gut microbiota and human NAFLD: disentangling microbial signatures from metabolic disorders","volume":"17","author":"Aron-Wisnewsky","year":"2020","journal-title":"Nat. Rev. Gastro. Hepat."},{"issue":"1","key":"10.1016\/j.actbio.2021.07.013_bib0002","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/s41575-020-00366-5","article-title":"Nonalcoholic steatohepatitis: the role of peroxisome proliferator-activated receptors","volume":"18","author":"Francque","year":"2021","journal-title":"Nat. Rev. Gastro. Hepat."},{"issue":"1","key":"10.1016\/j.actbio.2021.07.013_bib0003","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/nrgastro.2017.109","article-title":"Global burden of NAFLD and NASH: trends, predictions, risk factors and prevention","volume":"15","author":"Younossi","year":"2018","journal-title":"Nat. Rev. Gastro. Hepat."},{"issue":"1","key":"10.1016\/j.actbio.2021.07.013_bib0004","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/hep.28431","article-title":"Global epidemiology of nonalcoholic fatty liver disease-meta-analytic assessment of prevalence, incidence, and outcomes","volume":"64","author":"Younossi","year":"2016","journal-title":"Hepatology"},{"key":"10.1016\/j.actbio.2021.07.013_bib0005","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.phrs.2018.06.029","article-title":"Human-based systems: mechanistic NASH modelling just around the corner?","volume":"134","author":"Boeckmans","year":"2018","journal-title":"Pharmacol. Res."},{"issue":"1","key":"10.1016\/j.actbio.2021.07.013_bib0006","first-page":"117","article-title":"Advancing the understanding of NAFLD to hepatocellular carcinoma development: from experimental models to humans","volume":"1871","author":"Chen","year":"2019","journal-title":"Bba-Rev. Cancer"},{"key":"10.1016\/j.actbio.2021.07.013_bib0007","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejphar.2020.172913","article-title":"Challenges and opportunities in drug development for nonalcoholic steatohepatitis","volume":"870","author":"Ocker","year":"2020","journal-title":"Eur. J. Pharmacol."},{"issue":"2","key":"10.1016\/j.actbio.2021.07.013_bib0008","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1080\/17460441.2018.1410135","article-title":"Non-alcoholic fatty liver disease (NAFLD) models in drug discovery","volume":"13","author":"Cole","year":"2018","journal-title":"Expert Opin. Drug Dis."},{"issue":"3","key":"10.1016\/j.actbio.2021.07.013_bib0009","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.jhep.2016.05.005","article-title":"A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer","volume":"65","author":"Asgharpour","year":"2016","journal-title":"J. Hepatol."},{"key":"10.1016\/j.actbio.2021.07.013_bib0010","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.molmet.2019.01.013","article-title":"Pnpla3 silencing with antisense oligonucleotides ameliorates nonalcoholic steatohepatitis and fibrosis in Pnpla3 I148M knock-in mice","volume":"22","author":"Linden","year":"2019","journal-title":"Mol. Metab."},{"issue":"19","key":"10.1016\/j.actbio.2021.07.013_bib0011","doi-asserted-by":"crossref","first-page":"2300","DOI":"10.3748\/wjg.v18.i19.2300","article-title":"Animal models of nonalcoholic fatty liver disease\/nonalcoholic steatohepatitis","volume":"18","author":"Takahashi","year":"2012","journal-title":"World J. Gastroentero."},{"issue":"2","key":"10.1016\/j.actbio.2021.07.013_bib0012","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.jhep.2017.10.031","article-title":"Preclinical models of non-alcoholic fatty liver disease","volume":"68","author":"Santhekadur","year":"2018","journal-title":"J. Hepatol."},{"issue":"4","key":"10.1016\/j.actbio.2021.07.013_bib0013","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1002\/hep.28953","article-title":"Lipid zonation and phospholipid remodeling in nonalcoholic fatty liver disease","volume":"65","author":"Hall","year":"2017","journal-title":"Hepatology"},{"key":"10.1016\/j.actbio.2021.07.013_bib0014","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.taap.2016.04.007","article-title":"Advantageous use of HepaRG cells for the screening and mechanistic study of drug-induced steatosis","volume":"302","author":"Tolosa","year":"2016","journal-title":"Toxicol. Appl. Pharm."},{"key":"10.1016\/j.actbio.2021.07.013_bib0015","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.taap.2015.12.020","article-title":"A cellular model to study drug-induced liver injury in nonalcoholic fatty liver disease: application to acetaminophen","volume":"292","author":"Michaut","year":"2016","journal-title":"Toxicol. Appl. Pharm."},{"issue":"4","key":"10.1016\/j.actbio.2021.07.013_bib0016","first-page":"3659","article-title":"Influence of different concentrations of uric acid on oxidative stress in steatosis hepatocytes","volume":"15","author":"Cheng","year":"2018","journal-title":"Exp. Ther. Med."},{"issue":"4","key":"10.1016\/j.actbio.2021.07.013_bib0017","article-title":"Novel defatting strategies reduce lipid accumulation in primary human culture models of liver steatosis","volume":"13","author":"Aoudjehane","year":"2020","journal-title":"Dis. Model. Mech."},{"issue":"7","key":"10.1016\/j.actbio.2021.07.013_bib0018","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1016\/j.tiv.2015.07.010","article-title":"The interplay between hepatic stellate cells and hepatocytes in an in vitro model of NASH","volume":"29","author":"Barbero-Becerra","year":"2015","journal-title":"Toxicol. In Vitro"},{"issue":"2","key":"10.1016\/j.actbio.2021.07.013_bib0019","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.cmet.2019.05.007","article-title":"Modeling steatohepatitis in humans with pluripotent stem cell-derived organoids","volume":"30","author":"Ouchi","year":"2019","journal-title":"Cell Metab"},{"issue":"8","key":"10.1016\/j.actbio.2021.07.013_bib0020","article-title":"A human liver-on-a-chip platform for modeling nonalcoholic fatty liver disease","volume":"3","author":"Lasli","year":"2019","journal-title":"Adv. Biosyst."},{"issue":"7","key":"10.1016\/j.actbio.2021.07.013_bib0021","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.3390\/ijms20071629","article-title":"Human multilineage 3D spheroids as a model of liver steatosis and fibrosis","volume":"20","author":"Pingitore","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.actbio.2021.07.013_bib0022","doi-asserted-by":"crossref","first-page":"14297","DOI":"10.1038\/s41598-018-32722-6","article-title":"Human hepatic 3D spheroids as a model for steatosis and insulin resistance","volume":"8","author":"Kozyra","year":"2018","journal-title":"Sci. Rep."},{"issue":"24","key":"10.1016\/j.actbio.2021.07.013_bib0023","article-title":"In vitro human liver model of nonalcoholic steatohepatitis by coculturing hepatocytes, endothelial cells, and kupffer cells","volume":"8","author":"Suurmond","year":"2019","journal-title":"Adv. Healthc. Mater."},{"issue":"6","key":"10.1016\/j.actbio.2021.07.013_bib0024","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1016\/j.biomaterials.2013.11.021","article-title":"A review of microfabrication and hydrogel engineering for micro-organs on chips","volume":"35","author":"Verhulsel","year":"2014","journal-title":"Biomaterials"},{"issue":"8","key":"10.1016\/j.actbio.2021.07.013_bib0025","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1038\/nbt.2989","article-title":"Microfluidic organs-on-chips","volume":"32","author":"Bhatia","year":"2014","journal-title":"Nat. Biotechnol."},{"issue":"17","key":"10.1016\/j.actbio.2021.07.013_bib0026","doi-asserted-by":"crossref","first-page":"6402","DOI":"10.1039\/D0CS00705F","article-title":"Microfluidic lumen-based systems for advancing tubular organ modeling","volume":"49","author":"Virumbrales-Munoz","year":"2020","journal-title":"Chem. Soc. Rev."},{"issue":"7","key":"10.1016\/j.actbio.2021.07.013_bib0027","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0159729","article-title":"Investigating nonalcoholic fatty liver disease in a liver-on-a-chip microfluidic device","volume":"11","author":"Gori","year":"2016","journal-title":"Plos One"},{"issue":"1","key":"10.1016\/j.actbio.2021.07.013_bib0028","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1002\/bit.27557","article-title":"Quercetin and hydroxytyrosol as modulators of hepatic steatosis: a NAFLD-on-a-chip study","volume":"118","author":"Gori","year":"2021","journal-title":"Biotechnol. Bioeng."},{"issue":"2","key":"10.1016\/j.actbio.2021.07.013_bib0029","doi-asserted-by":"crossref","first-page":"204","DOI":"10.3748\/wjg.v23.i2.204","article-title":"Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease","volume":"23","author":"Kostrzewski","year":"2017","journal-title":"World J. Gastroentero."},{"issue":"18","key":"10.1016\/j.actbio.2021.07.013_bib0030","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1039\/C9LC00354A","article-title":"A microfluidic patterned model of non-alcoholic fatty liver disease: applications to disease progression and zonation","volume":"19","author":"Bututoglu","year":"2019","journal-title":"Lab. Chip"},{"issue":"20","key":"10.1016\/j.actbio.2021.07.013_bib0031","doi-asserted-by":"crossref","first-page":"e90954","DOI":"10.1172\/jci.insight.90954","article-title":"Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis","volume":"1","author":"Feaver","year":"2016","journal-title":"JCI Insight"},{"key":"10.1016\/j.actbio.2021.07.013_bib0032","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.tiv.2016.11.014","article-title":"Pharmacotoxicology of clinically-relevant concentrations of obeticholic acid in an organotypic human hepatocyte system","volume":"39","author":"Dash","year":"2017","journal-title":"Toxicol. In Vitro"},{"issue":"23","key":"10.1016\/j.actbio.2021.07.013_bib0033","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.201900075","article-title":"Implantable vascularized liver chip for cross-validation of disease treatment with animal model","volume":"29","author":"Lee","year":"2019","journal-title":"Adv. Funct. Mater."},{"issue":"1","key":"10.1016\/j.actbio.2021.07.013_bib0034","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.jhep.2016.07.009","article-title":"Liver sinusoidal endothelial cells: physiology and role in liver diseases","volume":"66","author":"Poisson","year":"2017","journal-title":"J. Hepatol."},{"issue":"9","key":"10.1016\/j.actbio.2021.07.013_bib0035","doi-asserted-by":"crossref","first-page":"222","DOI":"10.3390\/ph13090222","article-title":"Nonalcoholic fatty liver disease (NAFLD) and hepatic cytochrome P450 (CYP) enzymes","volume":"13","author":"Jamwal","year":"2020","journal-title":"Pharmaceuticals"},{"issue":"10","key":"10.1016\/j.actbio.2021.07.013_bib0036","doi-asserted-by":"crossref","first-page":"751","DOI":"10.2165\/00003088-200241100-00005","article-title":"Enterohepatic circulation - physiological, pharmacokinetic and clinical implications","volume":"41","author":"Roberts","year":"2002","journal-title":"Clin. Pharmacokinet."},{"issue":"23","key":"10.1016\/j.actbio.2021.07.013_bib0037","doi-asserted-by":"crossref","first-page":"10371","DOI":"10.1073\/pnas.0909374107","article-title":"Prediction and validation of cell alignment along microvessels as order principle to restore tissue architecture in liver regeneration","volume":"107","author":"Hoehme","year":"2010","journal-title":"P. Natl. Acad. Sci. USA"},{"issue":"2","key":"10.1016\/j.actbio.2021.07.013_bib0038","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.cmet.2019.06.017","article-title":"Generation of human fatty livers using custom-engineered induced pluripotent stem cells with modifiable SIRT1 metabolism","volume":"30","author":"de I'Hortet","year":"2019","journal-title":"Cell Metab."},{"issue":"23","key":"10.1016\/j.actbio.2021.07.013_bib0039","doi-asserted-by":"crossref","first-page":"4974","DOI":"10.1021\/ac980656z","article-title":"Rapid prototyping of microfluidic systems in poly(dimethylsiloxane)","volume":"70","author":"Duffy","year":"1998","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.actbio.2021.07.013_bib0040","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/(SICI)1522-2683(20000101)21:1<27::AID-ELPS27>3.0.CO;2-C","article-title":"Fabrication of microfluidic systems in poly(dimethylsiloxane)","volume":"21","author":"McDonald","year":"2000","journal-title":"Electrophoresis"},{"issue":"7","key":"10.1016\/j.actbio.2021.07.013_bib0041","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1038\/nprot.2012.051","article-title":"Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels","volume":"7","author":"Shin","year":"2012","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.actbio.2021.07.013_bib0042","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.actbio.2020.08.041","article-title":"Microfluidic organ-on-a-chip models of human liver tissue","volume":"116","author":"Moradi","year":"2020","journal-title":"Acta Biomater"},{"issue":"10","key":"10.1016\/j.actbio.2021.07.013_bib0043","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1038\/ni.2691","article-title":"Immune surveillance by the liver","volume":"14","author":"Jenne","year":"2013","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.actbio.2021.07.013_bib0044","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2021.120904","article-title":"A scalable and sensitive steatosis chip with long-term perfusion of in situ differentiated HepaRG organoids","volume":"275","author":"Teng","year":"2021","journal-title":"Biomaterials"},{"key":"10.1016\/j.actbio.2021.07.013_bib0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2020.120611","article-title":"3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model","volume":"269","author":"Cuvellier","year":"2021","journal-title":"Biomaterials"},{"key":"10.1016\/j.actbio.2021.07.013_bib0046","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.fct.2019.04.057","article-title":"Development of a two-layer transwell co-culture model for the in vitro investigation of pyrrolizidine alkaloid-induced hepatic sinusoidal damage","volume":"129","author":"Lu","year":"2019","journal-title":"Food Chem. Toxicol."},{"issue":"4","key":"10.1016\/j.actbio.2021.07.013_bib0047","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1002\/hep.21536","article-title":"Transdifferentiation of hepatocyte-like cells from the human hepatoma HepaRG cell line through bipotent progenitor","volume":"45","author":"Cerec","year":"2007","journal-title":"Hepatology"},{"issue":"24","key":"10.1016\/j.actbio.2021.07.013_bib0048","doi-asserted-by":"crossref","first-page":"15655","DOI":"10.1073\/pnas.232137699","article-title":"Infection of a human hepatoma cell line by hepatitis B virus","volume":"99","author":"Gripon","year":"2002","journal-title":"P. Natl. Acad. Sci. USA"},{"issue":"44","key":"10.1016\/j.actbio.2021.07.013_bib0049","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.201909553","article-title":"Engineering liver microtissues for disease modeling and regenerative medicine","volume":"30","author":"Huang","year":"2020","journal-title":"Adv. Funct. Mater."},{"issue":"5","key":"10.1016\/j.actbio.2021.07.013_bib0050","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1039\/C6LC01374K","article-title":"Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip","volume":"17","author":"Du","year":"2017","journal-title":"Lab. Chip"},{"issue":"17","key":"10.1016\/j.actbio.2021.07.013_bib0051","doi-asserted-by":"crossref","first-page":"2479","DOI":"10.1039\/C8LC00357B","article-title":"Microphysiological flux balance platform unravels the dynamics of drug induced steatosis","volume":"18","author":"Ehrlich","year":"2018","journal-title":"Lab. Chip"},{"issue":"4","key":"10.1016\/j.actbio.2021.07.013_bib0052","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0193664","article-title":"A practice-changing culture method relying on shaking substantially increases mitochondrial energy metabolism and functionality of human liver cell lines","volume":"13","author":"Adam","year":"2018","journal-title":"Plos One"},{"issue":"3","key":"10.1016\/j.actbio.2021.07.013_bib0053","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1124\/dmd.112.049098","article-title":"Phase 1 and phase 2 drug metabolism and bile acid production of HepaRG cells in a bioartificial liver in absence of dimethyl sulfoxide","volume":"41","author":"Hoekstra","year":"2013","journal-title":"Drug Metab. Dispos."},{"issue":"2018","key":"10.1016\/j.actbio.2021.07.013_bib0054","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1038\/s41467-018-02969-8","article-title":"3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection","volume":"9","author":"Ortega-Prieto","year":"2018","journal-title":"Nat. Commun."},{"issue":"36","key":"10.1016\/j.actbio.2021.07.013_bib0055","doi-asserted-by":"crossref","DOI":"10.1002\/adma.201701545","article-title":"Scaffold-free liver-on-a-chip with multiscale organotypic cultures","volume":"29","author":"Weng","year":"2017","journal-title":"Adv. Mater."},{"key":"10.1016\/j.actbio.2021.07.013_bib0056","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biomaterials.2015.11.026","article-title":"Novel human hepatic organoid model enables testing of drug-induced liver fibrosis in vitro","volume":"78","author":"Leite","year":"2016","journal-title":"Biomaterials"},{"issue":"9","key":"10.1016\/j.actbio.2021.07.013_bib0057","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1002\/jcp.24550","article-title":"Protective effect of the Y220C mutant p53 against steatosis: good news?","volume":"229","author":"Gori","year":"2014","journal-title":"J. Cell. Physiol."},{"issue":"10215","key":"10.1016\/j.actbio.2021.07.013_bib0058","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1016\/S0140-6736(19)33041-7","article-title":"Obeticholic acid for the treatment of non-alcoholic steatohepatitis: interim analysis from a multicentre, randomised, placebo-controlled phase 3 trial","volume":"394","author":"Younossi","year":"2019","journal-title":"Lancet"},{"issue":"1","key":"10.1016\/j.actbio.2021.07.013_bib0059","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1093\/bmb\/ldaa006","article-title":"Obeticholic acid-a new therapy in PBC and NASH","volume":"133","author":"Chapman","year":"2020","journal-title":"Brit. Med. Bull."},{"issue":"6","key":"10.1016\/j.actbio.2021.07.013_bib0060","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1111\/cts.12421","article-title":"Modeling and experimental studies of obeticholic acid exposure and the impact of cirrhosis stage","volume":"9","author":"Edwards","year":"2016","journal-title":"CTS-Clin. Transl. Sci."},{"key":"10.1016\/j.actbio.2021.07.013_bib0061","article-title":"Non-alcoholic fatty liver disease and steatohepatitis: state of the art on effective therapeutics based on the gold standard method for diagnosis","author":"Mahjoubin-Tehran","year":"2020","journal-title":"Mol. Metab."},{"issue":"5","key":"10.1016\/j.actbio.2021.07.013_bib0062","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1053\/j.gastro.2016.01.038","article-title":"Elafibranor, an agonist of the peroxisome proliferator-activated receptor-alpha and -delta, induces resolution of nonalcoholic steatohepatitis without fibrosis worsening","volume":"150","author":"Ratziu","year":"2016","journal-title":"Gastroenterology"},{"key":"10.1016\/j.actbio.2021.07.013_bib0063","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.phrs.2019.04.016","article-title":"Elafibranor restricts lipogenic and inflammatory responses in a human skin stem cell-derived model of NASH","volume":"144","author":"Boeckmans","year":"2019","journal-title":"Pharmacol. Res."},{"issue":"9","key":"10.1016\/j.actbio.2021.07.013_bib0064","doi-asserted-by":"crossref","DOI":"10.1242\/dmm.033530","article-title":"A nonalcoholic fatty liver disease model in human induced pluripotent stem cell-derived hepatocytes, created by endoplasmic reticulum stress-induced steatosis","volume":"11","author":"Parafati","year":"2018","journal-title":"Dis. Model. Mech."},{"issue":"11","key":"10.1016\/j.actbio.2021.07.013_bib0065","doi-asserted-by":"crossref","first-page":"2817","DOI":"10.1002\/bit.26793","article-title":"Gut-liver on a chip toward an in vitro model of hepatic steatosis","volume":"115","author":"Lee","year":"2018","journal-title":"Biotechnol. Bioeng."},{"issue":"3","key":"10.1016\/j.actbio.2021.07.013_bib0066","doi-asserted-by":"crossref","first-page":"446","DOI":"10.3109\/03602530903491881","article-title":"Sandwich-cultured hepatocytes: an in vitro model to evaluate hepatobiliary transporter-based drug interactions and hepatotoxicity","volume":"42","author":"Swift","year":"2010","journal-title":"Drug Metab. Rev."},{"issue":"2017","key":"10.1016\/j.actbio.2021.07.013_bib0067","article-title":"Design and fabrication of a scalable liver-lobule-on-a-chip microphysiological platform","volume":"9","author":"Banaeiyan","year":"2017","journal-title":"Biofabrication"},{"issue":"1","key":"10.1016\/j.actbio.2021.07.013_bib0068","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1093\/toxsci\/kfv041","article-title":"Comparative localization and functional activity of the main hepatobiliary transporters in HepaRG cells and primary human hepatocytes","volume":"145","author":"Bachour-El Azzi","year":"2015","journal-title":"Toxicol. Sci."},{"issue":"2","key":"10.1016\/j.actbio.2021.07.013_bib0069","doi-asserted-by":"crossref","first-page":"187","DOI":"10.2165\/00003088-200544020-00004","article-title":"The hepatic sinusoid in aging and cirrhosis: effects on hepatic substrate disposition and drug clearance","volume":"44","author":"Le Couteur","year":"2005","journal-title":"Clin. Pharmacokinet."},{"issue":"14","key":"10.1016\/j.actbio.2021.07.013_bib0070","article-title":"Bioengineered multicellular liver microtissues for modeling advanced hepatic fibrosis driven through non-alcoholic fatty liver disease","volume":"17","author":"Cho","year":"2021","journal-title":"Small"},{"issue":"6","key":"10.1016\/j.actbio.2021.07.013_bib0071","first-page":"643","article-title":"Nonalcoholic fatty liver disease\/nonalcoholic steatohepatitis: histological diagnostic criteria and scoring systems","volume":"22","author":"Tiniakos","year":"2010","journal-title":"Eur. J. Gastroen. Hepat."},{"issue":"10","key":"10.1016\/j.actbio.2021.07.013_bib0072","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1124\/dmd.109.027466","article-title":"Hepatic cytochrome P450 enzyme alterations in humans with progressive stages of nonalcoholic fatty liver disease","volume":"37","author":"Fisher","year":"2009","journal-title":"Drug Metab. Dispos."},{"issue":"5","key":"10.1016\/j.actbio.2021.07.013_bib0073","doi-asserted-by":"crossref","first-page":"487","DOI":"10.3390\/mi11050487","article-title":"The influence of chronic liver diseases on hepatic vasculature: a liver-on-a-chip review","volume":"11","author":"Ozkan","year":"2020","journal-title":"Micromachines"},{"issue":"2","key":"10.1016\/j.actbio.2021.07.013_bib0074","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1002\/hep.29554","article-title":"Zooming in on liver zonation","volume":"67","author":"Felmlee","year":"2018","journal-title":"Hepatology"},{"issue":"7","key":"10.1016\/j.actbio.2021.07.013_bib0075","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1038\/s41591-018-0104-9","article-title":"Mechanisms of NAFLD development and therapeutic strategies","volume":"24","author":"Friedman","year":"2018","journal-title":"Nat. Med."},{"key":"10.1016\/j.actbio.2021.07.013_bib0076","doi-asserted-by":"crossref","first-page":"8951","DOI":"10.1038\/s41598-018-27179-6","article-title":"Metabolic patterning on a chip: towards in vitro liver zonation of primary rat and human hepatocytes","volume":"8","author":"Kang","year":"2018","journal-title":"Sci. Rep."},{"issue":"4","key":"10.1016\/j.actbio.2021.07.013_bib0077","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1053\/j.gastro.2014.07.056","article-title":"Pathological features of fatty liver disease","volume":"147","author":"Yeh","year":"2014","journal-title":"Gastroenterology"},{"issue":"26","key":"10.1016\/j.actbio.2021.07.013_bib0078","doi-asserted-by":"crossref","first-page":"8491","DOI":"10.3748\/wjg.v20.i26.8491","article-title":"Liver zonation: novel aspects of its regulation and its impact on homeostasis","volume":"20","author":"Gebhardt","year":"2014","journal-title":"World J. Gastroentero."},{"issue":"1","key":"10.1016\/j.actbio.2021.07.013_bib0079","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/laban0108-41","article-title":"A portal vein cannulation technique for drug discovery in mice","volume":"37","author":"Shen","year":"2008","journal-title":"Lab Animal"},{"issue":"5","key":"10.1016\/j.actbio.2021.07.013_bib0080","article-title":"Advanced biomaterials and processing methods for liver regeneration: state-of-the-art and future trends","volume":"9","author":"Morais","year":"2020","journal-title":"Adv. Healthc. Mater."},{"issue":"46","key":"10.1016\/j.actbio.2021.07.013_bib0081","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.201703524","article-title":"InVADE: integrated vasculature for assessing dynamic events","volume":"27","author":"Lai","year":"2017","journal-title":"Adv. Funct. Mater."},{"issue":"43","key":"10.1016\/j.actbio.2021.07.013_bib0082","doi-asserted-by":"crossref","DOI":"10.1002\/adma.201801621","article-title":"Growth of epithelial organoids in a defined hydrogel","volume":"30","author":"Broguiere","year":"2018","journal-title":"Adv. Mater."},{"issue":"399","key":"10.1016\/j.actbio.2021.07.013_bib0083","doi-asserted-by":"crossref","first-page":"eaah5505","DOI":"10.1126\/scitranslmed.aah5505","article-title":"In situ expansion of engineered human liver tissue in a mouse model of chronic liver disease","volume":"9","author":"Stevens","year":"2017","journal-title":"Sci. Transl. Med."},{"issue":"18","key":"10.1016\/j.actbio.2021.07.013_bib0084","doi-asserted-by":"crossref","first-page":"4565","DOI":"10.1016\/j.biomaterials.2012.03.034","article-title":"Tissue specific synthetic ECM hydrogels for 3-D in vitro maintenance of hepatocyte function","volume":"33","author":"Skardal","year":"2012","journal-title":"Biomaterials"}],"container-title":["Acta Biomaterialia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S174270612100444X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S174270612100444X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T17:47:52Z","timestamp":1761673672000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S174270612100444X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10]]},"references-count":84,"alternative-id":["S174270612100444X"],"URL":"https:\/\/doi.org\/10.1016\/j.actbio.2021.07.013","relation":{},"ISSN":["1742-7061"],"issn-type":[{"value":"1742-7061","type":"print"}],"subject":[],"published":{"date-parts":[[2021,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply","name":"articletitle","label":"Article Title"},{"value":"Acta Biomaterialia","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actbio.2021.07.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}