{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:35:57Z","timestamp":1792650957937,"version":"4.5.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T00:00:00Z","timestamp":1653264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["015.013.010"],"award-info":[{"award-number":["015.013.010"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Biomaterialia"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1016\/j.actbio.2022.05.031","type":"journal-article","created":{"date-parts":[[2022,5,21]],"date-time":"2022-05-21T01:14:14Z","timestamp":1653095654000},"page":"50-62","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":67,"special_numbering":"C","title":["An in vitro model of fibrosis using crosslinked native extracellular matrix-derived hydrogels to modulate biomechanics without changing composition"],"prefix":"10.1016","volume":"147","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3663-4107","authenticated-orcid":false,"given":"Mehmet","family":"Nizamoglu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6931-2323","authenticated-orcid":false,"given":"Roderick H.J.","family":"de Hilster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenghua","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prashant K.","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo","family":"Borghuis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7128-2741","authenticated-orcid":false,"given":"Martin C.","family":"Harmsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9868-9966","authenticated-orcid":false,"given":"Janette K.","family":"Burgess","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.actbio.2022.05.031_bib0001","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.addr.2015.11.001","article-title":"Extracellular matrix structure","volume":"97","author":"Theocharis","year":"2016","journal-title":"Adv. Drug. Deliv. Rev."},{"issue":"Pt 24","key":"10.1016\/j.actbio.2022.05.031_bib0002","doi-asserted-by":"crossref","first-page":"4195","DOI":"10.1242\/jcs.023820","article-title":"The extracellular matrix at a glance","volume":"123","author":"Frantz","year":"2010","journal-title":"J. Cell Sci."},{"issue":"7","key":"10.1016\/j.actbio.2022.05.031_bib0003","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1016\/j.bbadis.2012.11.022","article-title":"Molecular assembly and mechanical properties of the extracellular matrix: a fibrous protein perspective","volume":"1832","author":"Muiznieks","year":"2013","journal-title":"Biochim. Biophys. Acta (BBA) Mol. Basis Dis."},{"issue":"4","key":"10.1016\/j.actbio.2022.05.031_bib0004","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1002\/path.4808","article-title":"The extracellular matrix - the under-recognized element in lung disease?","volume":"240","author":"Burgess","year":"2016","journal-title":"J. Pathol."},{"issue":"1","key":"10.1016\/j.actbio.2022.05.031_bib0005","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1172\/JCI93561","article-title":"Mechanosensing and fibrosis","volume":"128","author":"Tschumperlin","year":"2018","journal-title":"J. Clin. Invest."},{"issue":"4","key":"10.1016\/j.actbio.2022.05.031_bib0006","doi-asserted-by":"crossref","first-page":"L698","DOI":"10.1152\/ajplung.00451.2019","article-title":"Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue","volume":"318","author":"de Hilster","year":"2020","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"issue":"20","key":"10.1016\/j.actbio.2022.05.031_bib0007","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.1042\/CS20190893","article-title":"Regulation of cellular senescence by extracellular matrix during chronic fibrotic diseases","volume":"134","author":"Blokland","year":"2020","journal-title":"Clin. Sci."},{"issue":"4","key":"10.1016\/j.actbio.2022.05.031_bib0008","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1002\/path.5632","article-title":"Macrophage-stroma interactions in fibrosis: biochemical, biophysical, and cellular perspectives","volume":"254","author":"Vasse","year":"2021","journal-title":"J. Pathol."},{"key":"10.1016\/j.actbio.2022.05.031_bib0009","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.matbio.2017.12.004","article-title":"Matrix biomechanics and dynamics in pulmonary fibrosis","volume":"73","author":"Haak","year":"2018","journal-title":"Matrix Biol."},{"issue":"1","key":"10.1016\/j.actbio.2022.05.031_bib0010","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1172\/JCI93557","article-title":"Extracellular matrix as a driver of progressive fibrosis","volume":"128","author":"Herrera","year":"2018","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.actbio.2022.05.031_bib0011","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.matbio.2013.11.002","article-title":"Extracellular matrix proteins: a positive feedback loop in lung fibrosis?","volume":"34","author":"Blaauboer","year":"2014","journal-title":"Matrix Biol."},{"issue":"4","key":"10.1016\/j.actbio.2022.05.031_bib0012","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1172\/JCI71386","article-title":"Fibrotic extracellular matrix activates a profibrotic positive feedback loop","volume":"124","author":"Parker","year":"2014","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.actbio.2022.05.031_bib0013","article-title":"The multi-faceted extracellular matrix: unlocking its secrets for understanding the perpetuation of lung fibrosis","author":"Nizamoglu","year":"2022","journal-title":"Curr. Tissue Microenviron. Rep."},{"key":"10.1016\/j.actbio.2022.05.031_bib0014","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actbio.2016.11.068","article-title":"Extracellular matrix hydrogels from decellularized tissues: structure and function","volume":"49","author":"Saldin","year":"2017","journal-title":"Acta Biomater."},{"issue":"9","key":"10.1016\/j.actbio.2022.05.031_bib0015","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1164\/rccm.201204-0754OC","article-title":"Acellular normal and fibrotic human lung matrices as a culture system for in vitro investigation","volume":"186","author":"Booth","year":"2012","journal-title":"Am. J. Respir. Crit. Care Med."},{"issue":"31","key":"10.1016\/j.actbio.2022.05.031_bib0016","doi-asserted-by":"crossref","first-page":"6814","DOI":"10.1039\/D0TB00613K","article-title":"Clickable decellularized extracellular matrix as a new tool for building hybrid-hydrogels to model chronic fibrotic diseases in vitro","volume":"8","author":"Petrou","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.actbio.2022.05.031_bib0017","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.actbio.2020.11.029","article-title":"A photo-crosslinkable cartilage-derived extracellular matrix bioink for auricular cartilage tissue engineering","volume":"121","author":"Visscher","year":"2021","journal-title":"Acta Biomater."},{"issue":"21","key":"10.1016\/j.actbio.2022.05.031_bib0018","doi-asserted-by":"crossref","first-page":"5536","DOI":"10.1016\/j.biomaterials.2010.03.064","article-title":"Cell-laden microengineered gelatin methacrylate hydrogels","volume":"31","author":"Nichol","year":"2010","journal-title":"Biomaterials"},{"issue":"9","key":"10.1016\/j.actbio.2022.05.031_bib0019","doi-asserted-by":"crossref","first-page":"3475","DOI":"10.1021\/acs.biomac.9b00769","article-title":"Injectable shape-holding collagen hydrogel for cell encapsulation and delivery cross-linked using thiol-michael addition click reaction","volume":"20","author":"Pupkaite","year":"2019","journal-title":"Biomacromolecules"},{"issue":"4","key":"10.1016\/j.actbio.2022.05.031_bib0020","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.tibtech.2018.01.008","article-title":"Discovering cell-adhesion peptides in tissue engineering: beyond RGD","volume":"36","author":"Huettner","year":"2018","journal-title":"Trends Biotechnol."},{"issue":"1","key":"10.1016\/j.actbio.2022.05.031_bib0021","doi-asserted-by":"crossref","first-page":"10674","DOI":"10.1038\/s41598-019-47059-x","article-title":"Genipin and EDC crosslinking of extracellular matrix hydrogel derived from human umbilical cord for neural tissue repair","volume":"9","author":"Vyborny","year":"2019","journal-title":"Sci. Rep."},{"issue":"6","key":"10.1016\/j.actbio.2022.05.031_bib0022","doi-asserted-by":"crossref","DOI":"10.1002\/mabi.201900098","article-title":"Visible light cross-linking of gelatin hydrogels offers an enhanced cell microenvironment with improved light penetration depth","volume":"19","author":"Lim","year":"2019","journal-title":"Macromol. Biosci."},{"issue":"1","key":"10.1016\/j.actbio.2022.05.031_bib0023","doi-asserted-by":"crossref","DOI":"10.1063\/1.5067301","article-title":"Matrix crosslinking enhances macrophage adhesion, migration, and inflammatory activation","volume":"3","author":"Hsieh","year":"2019","journal-title":"APL Bioeng."},{"key":"10.1016\/j.actbio.2022.05.031_bib0024","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.actbio.2019.01.034","article-title":"Selective stiffening of fibrin hydrogels with micron resolution via photocrosslinking","volume":"87","author":"Keating","year":"2019","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2022.05.031_bib0025","article-title":"Light-activated decellularized extracellular matrix-based bioinks for volumetric tissue analogs at the centimeter scale","author":"Kim","year":"2021","journal-title":"Adv. Funct. Mater."},{"issue":"18","key":"10.1016\/j.actbio.2022.05.031_bib0026","doi-asserted-by":"crossref","DOI":"10.3390\/polym13183113","article-title":"Architecture and composition dictate viscoelastic properties of organ-derived extracellular matrix hydrogels","volume":"13","author":"Martinez-Garcia","year":"2021","journal-title":"Polymers"},{"issue":"8","key":"10.1016\/j.actbio.2022.05.031_bib0027","doi-asserted-by":"crossref","first-page":"1922","DOI":"10.1002\/jbm.a.35726","article-title":"Development and characterization of a naturally derived lung extracellular matrix hydrogel","volume":"104","author":"Pouliot","year":"2016","journal-title":"J. Biomed. Mater. Res. A"},{"issue":"6","key":"10.1016\/j.actbio.2022.05.031_bib0028","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1089\/ten.tec.2020.0042","article-title":"Porcine lung-derived extracellular matrix hydrogel properties are dependent on pepsin digestion time","volume":"26","author":"Pouliot","year":"2020","journal-title":"Tissue Eng. Part C Methods"},{"issue":"5","key":"10.1016\/j.actbio.2022.05.031_bib0029","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/j.exer.2011.08.009","article-title":"A comparative study on the viscoelastic properties of human and animal lenses","volume":"93","author":"Sharma","year":"2011","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.actbio.2022.05.031_bib0030","series-title":"Materials Science and Engineering: An Introduction","author":"Callister","year":"2017"},{"issue":"18","key":"10.1016\/j.actbio.2022.05.031_bib0031","doi-asserted-by":"crossref","first-page":"10153","DOI":"10.3390\/ijms221810153","article-title":"Adipose tissue-derived stromal cells alter the mechanical stability and viscoelastic properties of gelatine methacryloyl hydrogels","volume":"22","author":"Martinez-Garcia","year":"2021","journal-title":"Int. J. Mol. Sci."},{"issue":"3","key":"10.1016\/j.actbio.2022.05.031_bib0032","doi-asserted-by":"crossref","DOI":"10.26508\/lsa.202000880","article-title":"A FIJI macro for quantifying pattern in extracellular matrix","volume":"4","author":"Wershof","year":"2021","journal-title":"Life Sci Alliance"},{"issue":"7","key":"10.1016\/j.actbio.2022.05.031_bib0033","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.2005970","article-title":"CellProfiler 3.0: next-generation image processing for biology","volume":"16","author":"Mcquin","year":"2018","journal-title":"PLoS Biol."},{"issue":"4","key":"10.1016\/j.actbio.2022.05.031_bib0034","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1165\/rcmb.2012-0335OC","article-title":"Matrices of physiologic stiffness potently inactivate idiopathic pulmonary fibrosis fibroblasts","volume":"48","author":"Marinkovi\u0107","year":"2013","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"issue":"10","key":"10.1016\/j.actbio.2022.05.031_bib0035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0204765","article-title":"Cross-platform mechanical characterization of lung tissue","volume":"13","author":"Polio","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.actbio.2022.05.031_bib0036","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1038\/nmat4489","article-title":"Hydrogels with tunable stress relaxation regulate stem cell fate and activity","author":"Chaudhuri","year":"2016","journal-title":"Nat. Mater."},{"key":"10.1016\/j.actbio.2022.05.031_bib0037","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00497-13","article-title":"A distinguishable role of eDNA in the viscoelastic relaxation of biofilms","author":"Peterson","year":"2013","journal-title":"mBio"},{"issue":"163","key":"10.1016\/j.actbio.2022.05.031_bib0038","doi-asserted-by":"crossref","DOI":"10.1183\/16000617.0202-2021","article-title":"Chronic lung diseases: entangled in extracellular matrix","volume":"31","author":"Burgess","year":"2022","journal-title":"Eur. Respir. Rev."},{"issue":"11","key":"10.1016\/j.actbio.2022.05.031_bib0039","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1242\/dmm.030114","article-title":"Lysyl oxidases regulate fibrillar collagen remodelling in idiopathic pulmonary fibrosis","volume":"10","author":"Tjin","year":"2017","journal-title":"Dis. Model. Mech."},{"key":"10.1016\/j.actbio.2022.05.031_bib0040","doi-asserted-by":"crossref","DOI":"10.1002\/path.4808","article-title":"The extracellular matrix \u2013 the under-recognized element in lung disease?","author":"Burgess","year":"2016","journal-title":"J. Pathol."},{"issue":"5","key":"10.1016\/j.actbio.2022.05.031_bib0041","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1165\/rcmb.2016-0379OC","article-title":"Extracellular matrix cross-linking enhances fibroblast growth and protects against matrix proteolysis in lung fibrosis","volume":"58","author":"Philp","year":"2018","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"issue":"1","key":"10.1016\/j.actbio.2022.05.031_bib0042","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1117\/1.JBO.25.1.014505","article-title":"Probing ECM remodeling in idiopathic pulmonary fibrosis via second harmonic generation microscopy analysis of macro\/supramolecular collagen structure","volume":"25","author":"James","year":"2019","journal-title":"J. Biomed. Opt."},{"issue":"9","key":"10.1016\/j.actbio.2022.05.031_bib0043","doi-asserted-by":"crossref","DOI":"10.14814\/phy2.13281","article-title":"Matrix stiffness regulates migration of human lung fibroblasts","volume":"5","author":"Asano","year":"2017","journal-title":"Physiol. Rep."},{"issue":"4","key":"10.1016\/j.actbio.2022.05.031_bib0044","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1083\/jcb.201004082","article-title":"Feedback amplification of fibrosis through matrix stiffening and COX-2 suppression","volume":"190","author":"Liu","year":"2010","journal-title":"J. Cell Biol."},{"issue":"1","key":"10.1016\/j.actbio.2022.05.031_bib0045","doi-asserted-by":"crossref","first-page":"L59","DOI":"10.1152\/ajplung.00030.2018","article-title":"Greater cellular stiffness in fibroblasts from patients with idiopathic pulmonary fibrosis","volume":"315","author":"Jaffar","year":"2018","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"issue":"1","key":"10.1016\/j.actbio.2022.05.031_bib0046","doi-asserted-by":"crossref","DOI":"10.1186\/s12931-014-0155-5","article-title":"Increased constitutive \u03b1SMA and Smad2\/3 expression in idiopathic pulmonary fibrosis myofibroblasts is KCa3.1-dependent","volume":"15","author":"Roach","year":"2014","journal-title":"Respir. Res."},{"issue":"37","key":"10.1016\/j.actbio.2022.05.031_bib0047","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abb5069","article-title":"Microengineered 3D pulmonary interstitial mimetics highlight a critical role for matrix degradation in myofibroblast differentiation","volume":"6","author":"Matera","year":"2020","journal-title":"Sci. Adv."},{"issue":"1","key":"10.1016\/j.actbio.2022.05.031_bib0048","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-27346-9","article-title":"An immortalised mesenchymal stem cell line maintains mechano-responsive behaviour and can be used as a reporter of substrate stiffness","volume":"8","author":"Galarza Torre","year":"2018","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.actbio.2022.05.031_bib0049","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/nrm2594","article-title":"Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus","volume":"10","author":"Wang","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"6149","key":"10.1016\/j.actbio.2022.05.031_bib0050","doi-asserted-by":"crossref","DOI":"10.1126\/science.1240104","article-title":"Nuclear Lamin-A scales with tissue stiffness and enhances matrix-directed differentiation","volume":"341","author":"Swift","year":"2013","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.actbio.2022.05.031_bib0051","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ajpath.2020.09.008","article-title":"Mechanical feed-forward loops contribute to idiopathic pulmonary fibrosis","volume":"191","author":"Freeberg","year":"2021","journal-title":"Am. J. Pathol."},{"issue":"5","key":"10.1016\/j.actbio.2022.05.031_bib0052","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00497-13","article-title":"A distinguishable role of eDNA in the viscoelastic relaxation of biofilms","volume":"4","author":"Peterson","year":"2013","journal-title":"MBio"}],"container-title":["Acta Biomaterialia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1742706122003075?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1742706122003075?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T17:53:59Z","timestamp":1761674039000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1742706122003075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":52,"alternative-id":["S1742706122003075"],"URL":"https:\/\/doi.org\/10.1016\/j.actbio.2022.05.031","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.02.02.478812","asserted-by":"object"}]},"ISSN":["1742-7061"],"issn-type":[{"value":"1742-7061","type":"print"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An in vitro model of fibrosis using crosslinked native extracellular matrix-derived hydrogels to modulate biomechanics without changing composition","name":"articletitle","label":"Article Title"},{"value":"Acta Biomaterialia","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actbio.2022.05.031","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Ltd on behalf of Acta Materialia Inc.","name":"copyright","label":"Copyright"}]}}