{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T15:49:34Z","timestamp":1784389774618,"version":"3.55.0"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"am","delay-in-days":372,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM142875"],"award-info":[{"award-number":["R35GM142875"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Biomaterialia"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.actbio.2025.02.052","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T07:27:21Z","timestamp":1745306841000},"page":"37-48","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Macromolecular crowding-based biofabrication utilizing unmodified extracellular matrix bioinks"],"prefix":"10.1016","volume":"198","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0550-5772","authenticated-orcid":false,"given":"Seyma Nayir","family":"Jordan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianmu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Rossello-Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7376-9935","authenticated-orcid":false,"given":"Zixie","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugh","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6881-8130","authenticated-orcid":false,"given":"Michael","family":"Mak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"24","key":"10.1016\/j.actbio.2025.02.052_bib0001","doi-asserted-by":"crossref","first-page":"4195","DOI":"10.1242\/jcs.023820","article-title":"The extracellular matrix at a glance","volume":"123","author":"Frantz","year":"2010","journal-title":"J. Cell Sci."},{"issue":"5","key":"10.1016\/j.actbio.2025.02.052_bib0002","doi-asserted-by":"crossref","first-page":"914","DOI":"10.3390\/cells11050914","article-title":"Into the tissues: extracellular matrix and its artificial substitutes: cell signalling mechanisms","volume":"11","author":"Bandzerewicz","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.actbio.2025.02.052_bib0003","doi-asserted-by":"crossref","first-page":"S20","DOI":"10.1097\/IJG.0000000000000108","article-title":"Biology of the extracellular matrix: an overview","volume":"23","author":"Yue","year":"2014","journal-title":"J. Glaucoma"},{"issue":"12","key":"10.1016\/j.actbio.2025.02.052_bib0004","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a005058","article-title":"Extracellular matrix degradation and remodeling in development and disease","volume":"3","author":"Lu","year":"2011","journal-title":"Cold Spring Harb. Perspect. Biol."},{"issue":"1","key":"10.1016\/j.actbio.2025.02.052_bib0005","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s13036-022-00292-x","article-title":"Extracellular matrix dynamics: tracking in biological systems and their implications","volume":"16","author":"Hu","year":"2022","journal-title":"J. Biol. Eng."},{"key":"10.1016\/j.actbio.2025.02.052_bib0006","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2020.621644","article-title":"Bearing my heart: the role of extracellular matrix on cardiac development, homeostasis, and injury response","volume":"8","author":"Silva","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.actbio.2025.02.052_bib0007","doi-asserted-by":"crossref","first-page":"253","DOI":"10.3389\/fphys.2020.00253","article-title":"Skeletal muscle extracellular matrix \u2013 What do we know about its composition, regulation, and physiological roles? A narrative review","volume":"11","author":"Csapo","year":"2020","journal-title":"Front. Physiol."},{"issue":"7","key":"10.1016\/j.actbio.2025.02.052_bib0008","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1089\/ars.2013.5697","article-title":"Extracellular matrix and liver disease","volume":"21","author":"Arriazu","year":"2014","journal-title":"Antioxid. Redox Signal."},{"issue":"9","key":"10.1016\/j.actbio.2025.02.052_bib0009","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/nrgastro.2017.76","article-title":"The extracellular matrix of the gastrointestinal tract: a regenerative medicine platform","volume":"14","author":"Hussey","year":"2017","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"issue":"Suppl 1","key":"10.1016\/j.actbio.2025.02.052_bib0010","doi-asserted-by":"crossref","first-page":"S23","DOI":"10.5213\/inj.1632600.318","article-title":"Extracellular matrix revisited: roles in tissue engineering","volume":"20","author":"Kim","year":"2016","journal-title":"Int. Neurourol. J."},{"issue":"11","key":"10.1016\/j.actbio.2025.02.052_bib0011","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1016\/j.biomaterials.2007.12.014","article-title":"Preparation and rheological characterization of a gel form of the porcine urinary bladder matrix","volume":"29","author":"Freytes","year":"2008","journal-title":"Biomaterials"},{"key":"10.1016\/j.actbio.2025.02.052_bib0012","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/bs.mcb.2017.08.007","article-title":"Cell-derived decellularized extracellular matrices","volume":"143","author":"Harris","year":"2018","journal-title":"Methods Cell Biol"},{"issue":"24","key":"10.1016\/j.actbio.2025.02.052_bib0013","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202000734","article-title":"Designing decellularized extracellular matrix-based bioinks for 3D bioprinting","volume":"9","author":"Abaci","year":"2020","journal-title":"Adv. Healthc. Mater."},{"issue":"1","key":"10.1016\/j.actbio.2025.02.052_bib0014","doi-asserted-by":"crossref","first-page":"5658","DOI":"10.1038\/s41467-019-13605-4","article-title":"Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture","volume":"10","author":"Giobbe","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.actbio.2025.02.052_bib0015","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.actbio.2016.01.013","article-title":"Tailoring mechanical properties of decellularized extracellular matrix bioink by vitamin B2-induced photo-crosslinking","volume":"33","author":"Jang","year":"2016","journal-title":"Acta Biomater"},{"issue":"2","key":"10.1016\/j.actbio.2025.02.052_bib0016","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5090\/ab5d80","article-title":"Decellularized extracellular matrix-based bio-ink with enhanced 3D printability and mechanical properties","volume":"12","author":"Kim","year":"2020","journal-title":"Biofabrication"},{"key":"10.1016\/j.actbio.2025.02.052_bib0017","unstructured":"L. Lian et al., \u201cRapid volumetric bioprinting of decellularized extracellular matrix bioinks,\u201d Adv. Mater., vol. n\/a, no. n\/a, p. 2304846, 10.1002\/adma.202304846."},{"issue":"29","key":"10.1016\/j.actbio.2025.02.052_bib0018","doi-asserted-by":"crossref","first-page":"7028","DOI":"10.1016\/j.biomaterials.2012.06.051","article-title":"A hydrogel derived from decellularized dermal extracellular matrix","volume":"33","author":"Wolf","year":"2012","journal-title":"Biomaterials"},{"issue":"4","key":"10.1016\/j.actbio.2025.02.052_bib0019","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1039\/C7SM01933E","article-title":"Control of collagen gel mechanical properties through manipulation of gelation conditions near the sol\u2013gel transition","volume":"14","author":"Holder","year":"2018","journal-title":"Soft Matter."},{"issue":"4","key":"10.1016\/j.actbio.2025.02.052_bib0020","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.mattod.2016.12.005","article-title":"Tissue engineering by decellularization and 3D bioprinting","volume":"20","author":"Garreta","year":"2017","journal-title":"Mater. Today"},{"issue":"2","key":"10.1016\/j.actbio.2025.02.052_bib0021","doi-asserted-by":"crossref","DOI":"10.3390\/gels7020070","article-title":"Tunable Human myocardium derived decellularized extracellular matrix for 3D bioprinting and cardiac tissue engineering","volume":"7","author":"Basara","year":"2021","journal-title":"Gels"},{"key":"10.1016\/j.actbio.2025.02.052_bib0022","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.actbio.2020.11.006","article-title":"3D bioprinting of mechanically tuned bioinks derived from cardiac decellularized extracellular matrix","volume":"119","author":"Shin","year":"2021","journal-title":"Acta Biomater"},{"issue":"20","key":"10.1016\/j.actbio.2025.02.052_bib0023","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202203044","article-title":"3D Printing of extracellular matrix-based multicomponent, all-natural, highly elastic, and functional materials toward vascular tissue engineering","volume":"12","author":"Isik","year":"2023","journal-title":"Adv. Healthc. Mater."},{"issue":"11","key":"10.1016\/j.actbio.2025.02.052_bib0024","doi-asserted-by":"crossref","first-page":"5288","DOI":"10.1021\/acsbiomaterials.1c00812","article-title":"Decellularized extracellular matrix composite hydrogel bioinks for the development of 3D bioprinted head and neck in vitro tumor models","volume":"7","author":"Kort-Mascort","year":"2021","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"14","key":"10.1016\/j.actbio.2025.02.052_bib0025","doi-asserted-by":"crossref","first-page":"10577","DOI":"10.1074\/jbc.R100005200","article-title":"The influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological media *","volume":"276","author":"Minton","year":"2001","journal-title":"J. Biol. Chem."},{"issue":"4","key":"10.1016\/j.actbio.2025.02.052_bib0026","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1006\/jmbi.2001.5166","article-title":"Crowding and hydration effects on protein conformation: a study with sol-gel encapsulated proteins1","volume":"314","author":"Eggers","year":"2001","journal-title":"J. Mol. Biol."},{"issue":"48","key":"10.1016\/j.actbio.2025.02.052_bib0027","doi-asserted-by":"crossref","first-page":"9269","DOI":"10.1039\/C5TB02139A","article-title":"Macromolecular crowding and hydrophobic effects on fmoc-diphenylalanine hydrogel formation in PEG : water mixtures","volume":"3","author":"Hassan","year":"2015","journal-title":"J. Mater. Chem. B"},{"issue":"40","key":"10.1016\/j.actbio.2025.02.052_bib0028","doi-asserted-by":"crossref","first-page":"6344","DOI":"10.1039\/C8TB02201A","article-title":"Macromolecular crowding for materials-directed controlled self-assembly","volume":"6","author":"Hata","year":"2018","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.actbio.2025.02.052_bib0029","article-title":"Instant assembly of collagen for scaffolding","author":"Gong","year":"2023","journal-title":"Tissue Eng., Bioprint."},{"issue":"3","key":"10.1016\/j.actbio.2025.02.052_bib0030","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1073\/pnas.76.3.1274","article-title":"Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro","volume":"76","author":"Bell","year":"1979","journal-title":"Proc. Natl. Acad. Sci"},{"issue":"9","key":"10.1016\/j.actbio.2025.02.052_bib0031","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.1500758","article-title":"Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels","volume":"1","author":"Hinton","year":"2015","journal-title":"Sci. Adv."},{"issue":"9","key":"10.1016\/j.actbio.2025.02.052_bib0032","doi-asserted-by":"crossref","DOI":"10.3390\/cells10092443","article-title":"Fibronectin: molecular structure, fibrillar structure and mechanochemical signaling","volume":"10","author":"Dalton","year":"2021","journal-title":"Cells"},{"issue":"1","key":"10.1016\/j.actbio.2025.02.052_bib0033","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0022-2836(76)80035-6","article-title":"The macromolecular organization of the elastin fibril","volume":"100","author":"Serafini-Fracassini","year":"1976","journal-title":"J. Mol. Biol."},{"issue":"8","key":"10.1016\/j.actbio.2025.02.052_bib0034","article-title":"The mechanics of fibrillar collagen extracellular matrix","volume":"2","author":"Sun","year":"2021","journal-title":"Cell Rep. Phys. Sci."},{"issue":"4","key":"10.1016\/j.actbio.2025.02.052_bib0035","doi-asserted-by":"crossref","first-page":"240","DOI":"10.3390\/jfb13040240","article-title":"Preparation and use of decellularized extracellular matrix for tissue engineering","volume":"13","author":"McInnes","year":"2022","journal-title":"J. Funct. Biomater."},{"key":"10.1016\/j.actbio.2025.02.052_bib0036","first-page":"15","article-title":"Decellularized extracellular matrix scaffolds: recent trends and emerging strategies in tissue engineering","volume":"10","author":"Zhang","year":"2022","journal-title":"Bioact. Mater."},{"issue":"1","key":"10.1016\/j.actbio.2025.02.052_bib0037","doi-asserted-by":"crossref","first-page":"14933","DOI":"10.1038\/s41598-019-49575-2","article-title":"The impact of decellularization methods on extracellular matrix derived hydrogels","volume":"9","author":"Fern\u00e1ndez-P\u00e9rez","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.actbio.2025.02.052_bib0038","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2022.805299","article-title":"Decellularization in tissue engineering and regenerative medicine: evaluation, modification, and application methods","volume":"10","author":"Neishabouri","year":"2022","journal-title":"Front. Bioeng. Biotechnol"},{"issue":"2","key":"10.1016\/j.actbio.2025.02.052_bib0039","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1086\/340729","article-title":"A history of pepsin and related enzymes","volume":"77","author":"Fruton","year":"2002","journal-title":"Q. Rev. Biol."},{"key":"10.1016\/j.actbio.2025.02.052_bib0040","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2022\/6884370","article-title":"Proteomic analysis of decellularized extracellular matrix: achieving a competent biomaterial for osteogenesis","volume":"2022","author":"Monteiro-Lobato","year":"2022","journal-title":"BioMed. Res. Int."},{"issue":"10","key":"10.1016\/j.actbio.2025.02.052_bib0041","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1002\/adhm.201500035","article-title":"Partially digested adult cardiac extracellular matrix promotes cardiomyocyte proliferation In vitro","volume":"4","author":"Williams","year":"2015","journal-title":"Adv. Healthc. Mater."}],"container-title":["Acta Biomaterialia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1742706125001473?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1742706125001473?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T04:02:31Z","timestamp":1762056151000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1742706125001473"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":41,"alternative-id":["S1742706125001473"],"URL":"https:\/\/doi.org\/10.1016\/j.actbio.2025.02.052","relation":{},"ISSN":["1742-7061"],"issn-type":[{"value":"1742-7061","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Macromolecular crowding-based biofabrication utilizing unmodified extracellular matrix bioinks","name":"articletitle","label":"Article Title"},{"value":"Acta Biomaterialia","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actbio.2025.02.052","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Acta Materialia Inc. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}