{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:48:21Z","timestamp":1793062101517,"version":"4.5.0"},"reference-count":76,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"UK Research and Innovation Medical Research Council","doi-asserted-by":"publisher","award":["MR\/W019418\/1"],"award-info":[{"award-number":["MR\/W019418\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/S019367\/1"],"award-info":[{"award-number":["EP\/S019367\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/P025498\/1"],"award-info":[{"award-number":["EP\/P025498\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/L014904\/1"],"award-info":[{"award-number":["EP\/L014904\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/P025021\/1"],"award-info":[{"award-number":["EP\/P025021\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/R00661X\/1"],"award-info":[{"award-number":["EP\/R00661X\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000770","name":"University of Manchester","doi-asserted-by":"publisher","award":["204796\/Z\/16\/Z"],"award-info":[{"award-number":["204796\/Z\/16\/Z"]}],"id":[{"id":"10.13039\/501100000770","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Biomaterialia"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.actbio.2025.05.015","type":"journal-article","created":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T03:03:41Z","timestamp":1746587021000},"page":"378-389","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Suspension bioprinted whole intervertebral disc analogues enable regional stiffness- and hypoxia-regulated matrix secretion by primary human nucleus pulposus and annulus fibrosus cells"],"prefix":"10.1016","volume":"200","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0002-0197","authenticated-orcid":false,"given":"Matthew J.","family":"Kibble","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7529-6553","authenticated-orcid":false,"given":"Miguel J.S.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf H.","family":"Usta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7071-1487","authenticated-orcid":false,"given":"Guus G.H.","family":"van den Akker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel R.","family":"Moxon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Baird","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith A.","family":"Hoyland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco A.N.","family":"Domingos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7637-4135","authenticated-orcid":false,"given":"Stephen M.","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9859","key":"10.1016\/j.actbio.2025.05.015_bib0001","doi-asserted-by":"crossref","first-page":"2163","DOI":"10.1016\/S0140-6736(12)61729-2","article-title":"Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010","volume":"380","author":"Vos","year":"2012","journal-title":"Lancet"},{"key":"10.1016\/j.actbio.2025.05.015_bib0002","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1097\/00002517-200006000-00003","article-title":"The prevalence of low back pain: a systematic review of the literature from 1966 to 1998","author":"Walker","year":"2000","journal-title":"J. Spinal Disord."},{"issue":"9","key":"10.1016\/j.actbio.2025.05.015_bib0003","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1097\/BRS.0b013e3181a01b3f","article-title":"Prevalence and pattern of lumbar magnetic resonance imaging changes in a population study of one thousand forty-three individuals","volume":"34","author":"Cheung","year":"2009","journal-title":"Spine (Phila Pa 1976)"},{"issue":"5","key":"10.1016\/j.actbio.2025.05.015_bib0004","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1002\/jor.20789","article-title":"Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement","volume":"27","author":"Bron","year":"2009","journal-title":"J. Orthop. Res."},{"issue":"5","key":"10.1016\/j.actbio.2025.05.015_bib0005","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1002\/jor.1100170517","article-title":"Shear mechanical properties of human lumbar annulus fibrosus","volume":"17","author":"Iatridis","year":"1999","journal-title":"J. Orthop. Res."},{"issue":"10","key":"10.1016\/j.actbio.2025.05.015_bib0006","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1016\/S0021-9290(97)00069-9","article-title":"The viscoelastic behavior of the non-degenerate human lumbar nucleus pulposus in shear","volume":"30","author":"Iatridis","year":"1997","journal-title":"J. Biomech."},{"key":"10.1016\/j.actbio.2025.05.015_bib0007","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1097\/00007632-199605150-00009","article-title":"Is the nucleus pulposus a solid or a fluid? Mechanical behaviors of the nucleus pulposus of the human intervertebral disc","author":"Iatridis","year":"1996","journal-title":"Spine (Phila Pa 1976)"},{"issue":"2","key":"10.1016\/j.actbio.2025.05.015_bib0008","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1007\/s00586-007-0538-0","article-title":"Aggrecan, versican and type VI collagen are components of annular translamellar crossbridges in the intervertebral disc","volume":"17","author":"Melrose","year":"2008","journal-title":"Eur. Spine J."},{"issue":"Suppl 3","key":"10.1016\/j.actbio.2025.05.015_bib0009","article-title":"Mechanical loading of the intervertebral disc: from the macroscopic to the cellular level","volume":"23","author":"Neidlinger-Wilke","year":"2014","journal-title":"Eur. Spine J."},{"key":"10.1016\/j.actbio.2025.05.015_bib0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmbbm.2017.01.037","article-title":"Biomechanics of the human intervertebral disc: a review of testing techniques and results","author":"Newell","year":"2017","journal-title":"J. Mech. Behav. Biomed."},{"issue":"3","key":"10.1016\/j.actbio.2025.05.015_bib0011","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1115\/1.1374202","article-title":"Anisotropic and inhomogeneous tensile behavior of the human anulus fibrosus: experimental measurement and material model predictions","volume":"123","author":"Elliott","year":"2001","journal-title":"J. Biomech. Eng."},{"issue":"1","key":"10.1016\/j.actbio.2025.05.015_bib0012","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3109\/03008208909103905","article-title":"Hierarchical structure of the intervertebral disc","volume":"23","author":"Cassidy","year":"1989","journal-title":"Connect. Tissue Res."},{"issue":"13","key":"10.1016\/j.actbio.2025.05.015_bib0013","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23136915","article-title":"Importance of matrix cues on intervertebral disc development, degeneration, and regeneration","volume":"23","author":"Kibble","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"8","key":"10.1016\/j.actbio.2025.05.015_bib0014","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1016\/j.spinee.2016.03.046","article-title":"Diffusion characteristics of human annulus fibrosus-a study documenting the dependence of annulus fibrosus on end plate for diffusion","volume":"16","author":"Naresh-Babu","year":"2016","journal-title":"Spine J."},{"issue":"2","key":"10.1016\/j.actbio.2025.05.015_bib0015","doi-asserted-by":"crossref","first-page":"E131","DOI":"10.1097\/BRS.0b013e3181d52b9e","article-title":"Effect of oxygen levels on proteoglycan synthesis by intervertebral disc cells","volume":"36","author":"Mwale","year":"2011","journal-title":"Spine (Phila Pa 1976)"},{"issue":"3","key":"10.1016\/j.actbio.2025.05.015_bib0016","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.bbrc.2017.05.086","article-title":"Hypoxia-inducible factor-lalpha mediates aggrecan and collagen pi expression via NOTCH1 signaling in nucleus pulposus cells during intervertebral disc degeneration","volume":"488","author":"Liu","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"5","key":"10.1016\/j.actbio.2025.05.015_bib0018","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1002\/art.30246","article-title":"Hypoxia activates the notch signaling pathway in cells of the intervertebral disc: implications in degenerative disc disease","volume":"63","author":"Hiyama","year":"2011","journal-title":"Arthritis Rheum."},{"issue":"3","key":"10.1016\/j.actbio.2025.05.015_bib0019","doi-asserted-by":"crossref","DOI":"10.1002\/jsp2.1026","article-title":"Mechanotransduction and cell biomechanics of the intervertebral disc","volume":"1","author":"Fearing","year":"2018","journal-title":"JOR. Spine"},{"key":"10.1016\/j.actbio.2025.05.015_bib0020","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.actbio.2024.04.017","article-title":"Viscoelastic hydrogels regulate adipose-derived mesenchymal stem cells for nucleus pulposus regeneration","volume":"180","author":"Yin","year":"2024","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2025.05.015_bib0021","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.jot.2022.03.006","article-title":"Injectable hydrogel with nucleus pulposus-matched viscoelastic property prevents intervertebral disc degeneration","volume":"33","author":"Jia","year":"2022","journal-title":"J. Orthop. Translat."},{"issue":"7822","key":"10.1016\/j.actbio.2025.05.015_bib0022","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1038\/s41586-020-2612-2","article-title":"Effects of extracellular matrix viscoelasticity on cellular behaviour","volume":"584","author":"Chaudhuri","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.actbio.2025.05.015_bib0023","doi-asserted-by":"crossref","DOI":"10.1038\/s41578-019-0169-1","article-title":"The stiffness of living tissues and its implications for tissue engineering","author":"Guimar\u00e3es","year":"2020","journal-title":"Nature Rev. Mater."},{"issue":"1","key":"10.1016\/j.actbio.2025.05.015_bib0024","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1186\/s12967-023-04590-w","article-title":"Matrix stiffness induces Drp1-mediated mitochondrial fission through Piezo1 mechanotransduction in human intervertebral disc degeneration","volume":"21","author":"Ke","year":"2023","journal-title":"J. Transl. Med."},{"issue":"2","key":"10.1016\/j.actbio.2025.05.015_bib0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.yexcr.2022.113230","article-title":"Nucleus pulposus cell senescence is regulated by substrate stiffness and is alleviated by LOX possibly through the integrin beta1-p38 MAPK signaling pathway","volume":"417","author":"Zhao","year":"2022","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.actbio.2025.05.015_bib0026","article-title":"Mechanosensitive ion channel Piezo1 activated by matrix stiffness regulates oxidative stress-induced senescence and apoptosis in Human intervertebral disc degeneration","volume":"2021","author":"Wang","year":"2021","journal-title":"Oxid. Med. Cell Longev."},{"key":"10.1016\/j.actbio.2025.05.015_bib0027","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.actbio.2019.05.013","article-title":"Substrate stiffness-and topography-dependent differentiation of annulus fibrosus-derived stem cells is regulated by Yes-associated protein","volume":"92","author":"Chu","year":"2019","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2025.05.015_bib0028","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.actbio.2015.09.039","article-title":"Modulation of the gene expression of annulus fibrosus-derived stem cells using poly (ether carbonate urethane) urea scaffolds of tunable elasticity","volume":"29","author":"Zhu","year":"2016","journal-title":"Acta Biomater."},{"issue":"2","key":"10.1016\/j.actbio.2025.05.015_bib0029","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.matbio.2010.10.008","article-title":"Substrate stiffness regulates apoptosis and the mRNA expression of extracellular matrix regulatory genes in the rat annular cells","volume":"30","author":"Zhang","year":"2011","journal-title":"Matrix Biol."},{"issue":"3","key":"10.1016\/j.actbio.2025.05.015_bib0030","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/s00441-019-03136-1","article-title":"Mechanobiology of annulus fibrosus and nucleus pulposus cells in intervertebral discs","volume":"379","author":"Molladavoodi","year":"2020","journal-title":"Cell Tissue Res."},{"issue":"5","key":"10.1016\/j.actbio.2025.05.015_bib0017","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1093\/neuros\/nyx149","article-title":"The role of hypoxia in angiogenesis and extracellular matrix regulation of intervertebral disc cells during inflammatory reactions","volume":"81","author":"Kwon","year":"2017","journal-title":"Neurosurgery."},{"issue":"2","key":"10.1016\/j.actbio.2025.05.015_bib0031","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.yexmp.2011.06.001","article-title":"Variations in aggrecan localization and gene expression patterns characterize increasing stages of human intervertebral disk degeneration","volume":"91","author":"Gruber","year":"2011","journal-title":"Exp. Mol. Pathol."},{"issue":"10","key":"10.1016\/j.actbio.2025.05.015_bib0032","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1097\/01.brs.0000162624.95262.73","article-title":"Human intervertebral disc aggrecan inhibits endothelial cell adhesion and cell migration in vitro","volume":"30","author":"Johnson","year":"2005","journal-title":"Spine (Phila Pa 1976)"},{"key":"10.1016\/j.actbio.2025.05.015_bib0033","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/978-3-7091-1535-0_26","article-title":"Tissue engineering of the intervertebral disc","author":"Kandel","year":"2014","journal-title":"Intervertebral Disc"},{"issue":"1","key":"10.1016\/j.actbio.2025.05.015_bib0034","first-page":"179","article-title":"A 3D-bioprinted dual growth factor-releasing intervertebral disc scaffold induces nucleus pulposus and annulus fibrosus reconstruction","volume":"6","author":"Sun","year":"2021","journal-title":"Bioact. Mater."},{"issue":"7","key":"10.1016\/j.actbio.2025.05.015_bib0035","doi-asserted-by":"crossref","DOI":"10.1002\/smll.201702582","article-title":"Reverse reconstruction and bioprinting of bacterial cellulose-based functional total intervertebral disc for therapeutic implantation","volume":"14","author":"Yang","year":"2018","journal-title":"Small."},{"key":"10.1016\/j.actbio.2025.05.015_bib0036","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.actbio.2020.05.039","article-title":"Fabrication, maturation, and implantation of composite tissue-engineered total discs formed from native and mesenchymal stem cell combinations","volume":"114","author":"Kim","year":"2020","journal-title":"Acta Biomater."},{"issue":"32","key":"10.1016\/j.actbio.2025.05.015_bib0037","doi-asserted-by":"crossref","first-page":"13106","DOI":"10.1073\/pnas.1107094108","article-title":"Tissue-engineered intervertebral discs produce new matrix, maintain disc height, and restore biomechanical function to the rodent spine","volume":"108","author":"Bowles","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.actbio.2025.05.015_bib0038","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.msec.2018.11.087","article-title":"Engineering a biomimetic integrated scaffold for intervertebral disc replacement","volume":"96","author":"Du","year":"2019","journal-title":"Mater. Sci. Eng. C. Mater. Biol. Appl."},{"issue":"2","key":"10.1016\/j.actbio.2025.05.015_bib0039","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1080\/03008207.2019.1650037","article-title":"Customised multiphasic nucleus\/annulus scaffold for intervertebral disc repair\/regeneration","volume":"61","author":"Gloria","year":"2020","journal-title":"Connect. Tissue Res."},{"issue":"4","key":"10.1016\/j.actbio.2025.05.015_bib0040","doi-asserted-by":"crossref","DOI":"10.1002\/sstr.202200254","article-title":"Biomimetic design of 3D fibrous mesh reinforced hydrogel replicating the form and function of the intervertebral disc","volume":"4","author":"Lu","year":"2023","journal-title":"Small. Struct."},{"issue":"7","key":"10.1016\/j.actbio.2025.05.015_bib0041","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1002\/jbm.b.34323","article-title":"The establishment and biological assessment of a whole tissue-engineered intervertebral disc with PBST fibers and a chitosan hydrogel in vitro and in vivo","volume":"107","author":"Yuan","year":"2019","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"issue":"19","key":"10.1016\/j.actbio.2025.05.015_bib0042","doi-asserted-by":"crossref","first-page":"10793","DOI":"10.1021\/acs.chemrev.0c00008","article-title":"Inkjet Bioprinting of biomaterials","volume":"120","author":"Li","year":"2020","journal-title":"Chem. Rev."},{"issue":"5","key":"10.1016\/j.actbio.2025.05.015_bib0043","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1039\/C7BM00765E","article-title":"Bioinks for 3D bioprinting: an overview","volume":"6","author":"Gungor-Ozkerim","year":"2018","journal-title":"Biomater. Sci."},{"issue":"1","key":"10.1016\/j.actbio.2025.05.015_bib0044","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.cell.2020.12.002","article-title":"Bioprinting for the biologist","volume":"184","author":"Daly","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.actbio.2025.05.015_bib0045","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.mtcomm.2018.01.011","article-title":"Engineering patient-specific bioprinted constructs for treatment of degenerated intervertebral disc","volume":"19","author":"Costa","year":"2019","journal-title":"Mater. Today Commun."},{"issue":"4","key":"10.1016\/j.actbio.2025.05.015_bib0046","doi-asserted-by":"crossref","first-page":"e1117","DOI":"10.1002\/jsp2.1117","article-title":"Electrospinning and 3D bioprinting for intervertebral disc tissue engineering","volume":"3","author":"De Pieri","year":"2020","journal-title":"JOR. Spine"},{"key":"10.1016\/j.actbio.2025.05.015_bib0047","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.matlet.2018.03.204","article-title":"3D bioprinting of cell-laden scaffolds for intervertebral disc regeneration","volume":"223","author":"Hu","year":"2018","journal-title":"Mater. Lett."},{"issue":"13\u201314","key":"10.1016\/j.actbio.2025.05.015_bib0048","article-title":"3D bioprinting whole intervertebral discs to understand development & inform regenerative therapies","volume":"29","author":"Kibble","year":"2023","journal-title":"Tissue Eng. Pt A"},{"key":"10.1016\/j.actbio.2025.05.015_bib0049","doi-asserted-by":"crossref","first-page":"10661","DOI":"10.2147\/IJN.S469302","article-title":"Revolutionizing intervertebral disc regeneration: advances and future directions in three-dimensional bioprinting of Hydrogel scaffolds","volume":"19","author":"Zhang","year":"2024","journal-title":"Int. J. Nanomed."},{"issue":"1","key":"10.1016\/j.actbio.2025.05.015_bib0050","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5090\/ad8379","article-title":"3D bioprinting of an intervertebral disc tissue analogue with a highly aligned annulus fibrosus via suspended layer additive manufacture","volume":"17","author":"Moxon","year":"2024","journal-title":"Biofabrication."},{"issue":"49","key":"10.1016\/j.actbio.2025.05.015_bib0051","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.201904845","article-title":"Fabrication of complex hydrogel structures using suspended layer additive manufacturing (SLAM)","volume":"29","author":"Senior","year":"2019","journal-title":"Adv. Funct. Mater."},{"issue":"19","key":"10.1016\/j.actbio.2025.05.015_bib0052","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21197143","article-title":"Regenerative response of degenerate Human nucleus pulposus cells to GDF6 stimulation","volume":"21","author":"Hodgkinson","year":"2020","journal-title":"Int. J. Mol. Sci."},{"issue":"12","key":"10.1016\/j.actbio.2025.05.015_bib0053","doi-asserted-by":"crossref","first-page":"3695","DOI":"10.1002\/art.27710","article-title":"Characterization of the human nucleus pulposus cell phenotype and evaluation of novel marker gene expression to define adult stem cell differentiation","volume":"62","author":"Minogue","year":"2010","journal-title":"Arthritis Rheum."},{"issue":"3","key":"10.1016\/j.actbio.2025.05.015_bib0054","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s10237-004-0053-8","article-title":"Single lamellar mechanics of the human lumbar anulus fibrosus","volume":"3","author":"Holzapfel","year":"2005","journal-title":"Biomech. Model. Mechanobiol."},{"issue":"3","key":"10.1016\/j.actbio.2025.05.015_bib0055","doi-asserted-by":"crossref","first-page":"R135","DOI":"10.1186\/ar4597","article-title":"Novel immortal human cell lines reveal subpopulations in the nucleus pulposus","volume":"16","author":"van den Akker","year":"2014","journal-title":"Arthritis Res. Ther."},{"issue":"1","key":"10.1016\/j.actbio.2025.05.015_bib0056","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0144497","article-title":"Novel immortal cell lines support cellular heterogeneity in the Human annulus fibrosus","volume":"11","author":"van den Akker","year":"2016","journal-title":"PLoS. One"},{"issue":"2","key":"10.1016\/j.actbio.2025.05.015_bib0057","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1136\/mp.55.2.91","article-title":"Expression of chondrocyte markers by cells of normal and degenerate intervertebral discs","volume":"55","author":"Sive","year":"2002","journal-title":"Mol. Pathol."},{"issue":"5","key":"10.1016\/j.actbio.2025.05.015_bib0058","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1038\/nmeth.3839","article-title":"A practical guide to hydrogels for cell culture","volume":"13","author":"Caliari","year":"2016","journal-title":"Nat. Methods"},{"issue":"3","key":"10.1016\/j.actbio.2025.05.015_bib0059","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/BF02427846","article-title":"Comparison of the structure of human intervertebral discs in the cervical, thoracic and lumbar regions of the spine","volume":"8","author":"Pooni","year":"1986","journal-title":"Surg. Radiol. Anat."},{"issue":"1","key":"10.1016\/j.actbio.2025.05.015_bib0060","article-title":"Effects of extrusion pressure and printing speed of 3D bioprinted construct on the fibroblast cells viability","volume":"440","author":"Fakhruddin","year":"2018","journal-title":"IOP Conf. Ser.: Mater. Sci. Eng."},{"issue":"1","key":"10.1016\/j.actbio.2025.05.015_bib0061","first-page":"70","article-title":"A review on cell damage, viability, and functionality during 3D bioprinting","volume":"9","author":"Xu","year":"2022","journal-title":"Mil. Med. Res."},{"issue":"4","key":"10.1016\/j.actbio.2025.05.015_bib0062","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1039\/C6BM00861E","article-title":"Microvalve-based bioprinting - process, bio-inks and applications","volume":"5","author":"Ng","year":"2017","journal-title":"Biomater. Sci."},{"key":"10.1016\/j.actbio.2025.05.015_bib0063","doi-asserted-by":"crossref","DOI":"10.1016\/j.msec.2019.109904","article-title":"Blended alginate\/collagen hydrogels promote neurogenesis and neuronal maturation","volume":"104","author":"Moxon","year":"2019","journal-title":"Mater. Sci. Eng. C. Mater. Biol. Appl."},{"key":"10.1016\/j.actbio.2025.05.015_bib0064","article-title":"Hydrogel-based 3D bioprinting: a comprehensive review on cell-laden hydrogels, bioink formulations, and future perspectives","volume":"18","author":"Unagolla","year":"2020","journal-title":"Appl. Mater. Today"},{"issue":"4","key":"10.1016\/j.actbio.2025.05.015_bib0065","doi-asserted-by":"crossref","DOI":"10.1063\/5.0061361","article-title":"A suspended layer additive manufacturing approach to the bioprinting of tri-layered skin equivalents","volume":"5","author":"Moakes","year":"2021","journal-title":"APL. Bioeng."},{"issue":"8","key":"10.1016\/j.actbio.2025.05.015_bib0066","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1038\/s41563-019-0307-6","article-title":"Local nascent protein deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels","volume":"18","author":"Loebel","year":"2019","journal-title":"Nat. Mater."},{"issue":"3","key":"10.1016\/j.actbio.2025.05.015_bib0067","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1038\/s41596-021-00652-9","article-title":"Metabolic labeling of secreted matrix to investigate cell-material interactions in tissue engineering and mechanobiology","volume":"17","author":"Loebel","year":"2022","journal-title":"Nat. Protoc."},{"issue":"8","key":"10.1016\/j.actbio.2025.05.015_bib0068","doi-asserted-by":"crossref","first-page":"734","DOI":"10.3349\/ymj.2021.62.8.734","article-title":"Hypoxia regulates the extracellular matrix via mitogen-activated protein kinases pathway in cells retrieved from the Human intervertebral disc","volume":"62","author":"Kang","year":"2021","journal-title":"Yonsei. Med. J."},{"key":"10.1016\/j.actbio.2025.05.015_bib0069","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.addr.2014.06.009","article-title":"Advancing the cellular and molecular therapy for intervertebral disc disease","volume":"84","author":"Sakai","year":"2015","journal-title":"Adv. Drug Deliv. Rev."},{"issue":"2","key":"10.1016\/j.actbio.2025.05.015_bib0070","doi-asserted-by":"crossref","first-page":"C621","DOI":"10.1152\/ajpcell.00538.2006","article-title":"Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk","volume":"293","author":"Agrawal","year":"2007","journal-title":"Am. J. Physiol. Cell Physiol."},{"issue":"18","key":"10.1016\/j.actbio.2025.05.015_bib0071","doi-asserted-by":"crossref","first-page":"12654","DOI":"10.1074\/jbc.M112.448860","article-title":"Hypoxia-inducible factor (HIF)-1alpha and CCN2 form a regulatory circuit in hypoxic nucleus pulposus cells: CCN2 suppresses HIF-1alpha level and transcriptional activity","volume":"288","author":"Tran","year":"2013","journal-title":"J. Biol. Chem."},{"issue":"15","key":"10.1016\/j.actbio.2025.05.015_bib0072","first-page":"7144","article-title":"Influence of angiopoietin treatment with hypoxia and normoxia on Human intervertebral disc progenitor cell's proliferation","volume":"11","author":"Bischof","year":"2021","journal-title":"Metabolic Activ. Phenotype, Appl. Sci.-Basel"},{"key":"10.1016\/j.actbio.2025.05.015_bib0074","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.actbio.2019.05.004","article-title":"Graphene oxide containing self-assembling peptide hybrid hydrogels as a potential 3D injectable cell delivery platform for intervertebral disc repair applications","volume":"92","author":"Ligorio","year":"2019","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2025.05.015_bib0075","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.actbio.2021.03.077","article-title":"TGF-beta3-loaded graphene oxide - self-assembling peptide hybrid hydrogels as functional 3D scaffolds for the regeneration of the nucleus pulposus","volume":"127","author":"Ligorio","year":"2021","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2025.05.015_bib0076","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2022.842525","article-title":"Current insights into the maintenance of structure and function of intervertebral disc: a review of the regulatory role of growth and differentiation factor-5","volume":"13","author":"Lv","year":"2022","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.actbio.2025.05.015_bib0077","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2022.121405","article-title":"Integrating melt electrowriting and inkjet bioprinting for engineering structurally organized articular cartilage","volume":"283","author":"Dufour","year":"2022","journal-title":"Biomaterials"}],"container-title":["Acta Biomaterialia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S174270612500340X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S174270612500340X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T08:53:12Z","timestamp":1774860792000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S174270612500340X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":76,"alternative-id":["S174270612500340X"],"URL":"https:\/\/doi.org\/10.1016\/j.actbio.2025.05.015","relation":{},"ISSN":["1742-7061"],"issn-type":[{"value":"1742-7061","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Suspension bioprinted whole intervertebral disc analogues enable regional stiffness- and hypoxia-regulated matrix secretion by primary human nucleus pulposus and annulus fibrosus cells","name":"articletitle","label":"Article Title"},{"value":"Acta Biomaterialia","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actbio.2025.05.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc. on behalf of Acta Materialia Inc.","name":"copyright","label":"Copyright"}]}}