{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T09:35:05Z","timestamp":1793266505071,"version":"4.5.0"},"reference-count":102,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,16]],"date-time":"2026-12-16T00:00:00Z","timestamp":1797379200000},"content-version":"am","delay-in-days":349,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["P500PN210723"],"award-info":[{"award-number":["P500PN210723"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000070","name":"National Institute of Biomedical Imaging and Bioengineering","doi-asserted-by":"publisher","award":["K01-EB033870"],"award-info":[{"award-number":["K01-EB033870"]}],"id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["P30EY026877"],"award-info":[{"award-number":["P30EY026877"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY033363"],"award-info":[{"award-number":["R01EY033363"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01-EY035697"],"award-info":[{"award-number":["R01-EY035697"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["F31-EY034785"],"award-info":[{"award-number":["F31-EY034785"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DMR-2427971"],"award-info":[{"award-number":["DMR-2427971"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE-2146755"],"award-info":[{"award-number":["DGE-2146755"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["ECCS-2026822"],"award-info":[{"award-number":["ECCS-2026822"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008227","name":"Achievement Rewards for College Scientists Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008227","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Biomaterialia"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.actbio.2025.11.013","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:17:43Z","timestamp":1763036263000},"page":"167-182","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Dual-orientation of collagen fibers to guide cell alignment in 3D-printed constructs"],"prefix":"10.1016","volume":"209","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2827-6485","authenticated-orcid":false,"given":"Diya","family":"Singhal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7383-5875","authenticated-orcid":false,"given":"Fotis","family":"Christakopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0327-5635","authenticated-orcid":false,"given":"Lucia G.","family":"Brunel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suraj","family":"Borkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4800-3692","authenticated-orcid":false,"given":"Vanessa M.","family":"Doulames","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7927-024X","authenticated-orcid":false,"given":"Esther A.T.","family":"Mozipo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Myung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald G.","family":"Fuller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9801-6304","authenticated-orcid":false,"given":"Sarah C.","family":"Heilshorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"12","key":"10.1016\/j.actbio.2025.11.013_b1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/S1369-7021(06)71742-4","article-title":"Fibrous proteins and tissue engineering","volume":"9","author":"Wang","year":"2006","journal-title":"Mater. Today"},{"key":"10.1016\/j.actbio.2025.11.013_b2","doi-asserted-by":"crossref","DOI":"10.3389\/fams.2021.680029","article-title":"Collective Cell Migration in a Fibrous Environment: A Hybrid Multiscale Modelling Approach","volume":"7","author":"Suveges","year":"2021","journal-title":"Front. Appl. Math. Stat."},{"key":"10.1016\/j.actbio.2025.11.013_b3","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2024.1354132","article-title":"Modeling the extracellular matrix in cell migration and morphogenesis: a guide for the curious biologist","volume":"12","author":"Crossley","year":"2024","journal-title":"Front. Cell Dev. Biol."},{"issue":"5","key":"10.1016\/j.actbio.2025.11.013_b4","doi-asserted-by":"crossref","first-page":"3200","DOI":"10.1021\/la803736x","article-title":"Liquid crystalline collagen: A self-assembled morphology for the orientation of mammalian cells","volume":"25","author":"Kirkwood","year":"2009","journal-title":"Langmuir"},{"key":"10.1016\/j.actbio.2025.11.013_b5","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.exer.2014.07.018","article-title":"From nano to macro: Studying the hierarchical structure of the corneal extracellular matrix","volume":"133","author":"Quantock","year":"2015","journal-title":"Exp. Eye Res."},{"issue":"22","key":"10.1016\/j.actbio.2025.11.013_b6","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202301353","article-title":"Collagen Fibril Orientation Instructs Fibroblast Differentiation Via Cell Contractility","volume":"10","author":"Sapudom","year":"2023","journal-title":"Adv. Sci."},{"key":"10.1016\/j.actbio.2025.11.013_b7","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5090\/ab1a8b","article-title":"Shear-induced alignment of collagen fibrils using 3D cell printing for corneal stroma tissue engineering","volume":"11","author":"Kim","year":"2019","journal-title":"Biofabrication"},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b8","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1111\/os.12627","article-title":"Picrosirius-Polarization Method for Collagen Fiber Detection in Tendons: A Mini-Review","volume":"13","author":"Liu","year":"2021","journal-title":"Orthop. Surg."},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b9","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1016\/j.bpj.2017.06.046","article-title":"Dynamics of Tissue-Induced Alignment of Fibrous Extracellular Matrix","volume":"113","author":"Piotrowski-Daspit","year":"2017","journal-title":"Biophys. J."},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b10","doi-asserted-by":"crossref","first-page":"270","DOI":"10.18063\/ijb.v6i3.270","article-title":"Collagen as bioink for bioprinting: A comprehensive review","volume":"6","author":"Osidak","year":"2020","journal-title":"Int. J. Bioprinting"},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b11","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1021\/acsbiomaterials.2c00730","article-title":"Collagen-Based Biomaterials for Tissue Engineering","volume":"9","author":"Wang","year":"2023","journal-title":"ACS Biomater. Sci. Eng."},{"key":"10.1016\/j.actbio.2025.11.013_b12","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-82441-4","article-title":"Tissue growth as a mechanism for collagen fiber alignment in articular cartilage","volume":"14","author":"Peters","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.actbio.2025.11.013_b13","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1007\/s10439-007-9340-8","article-title":"An ultrastructural analysis of collagen in tissue engineered arteries","volume":"35","author":"Dahl","year":"2007","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.actbio.2025.11.013_b14","doi-asserted-by":"crossref","first-page":"7307","DOI":"10.1073\/pnas.111150598","article-title":"Corneal collagen fibril structure in three dimensions: Structural insights into fibril assembly, mechanical properties, and tissue organization","volume":"98","author":"Holmes","year":"2001","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.actbio.2025.11.013_b15","doi-asserted-by":"crossref","first-page":"3305","DOI":"10.1242\/jeb.202.23.3305","article-title":"Mechanical Design in Arteries","volume":"202","author":"Shadwick","year":"1999","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.actbio.2025.11.013_b16","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.actbio.2021.04.053","article-title":"Directional cues in the tumor microenvironment due to cell contraction against aligned collagen fibers","volume":"129","author":"Szulczewski","year":"2021","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2025.11.013_b17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41563-022-01259-5","article-title":"Self-generated gradients steer collective migration on viscoelastic collagen networks","volume":"21","author":"Clark","year":"2022","journal-title":"Nat. Mater."},{"issue":"21","key":"10.1016\/j.actbio.2025.11.013_b18","doi-asserted-by":"crossref","first-page":"11858","DOI":"10.1002\/anie.202016084","article-title":"Dynamically re-organized collagen fiber bundles transmit mechanical signals and induce strongly correlated cell migration and self-organization","volume":"60","author":"Fan","year":"2021","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.actbio.2025.11.013_b19","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.actbio.2025.03.007","article-title":"Spontaneous bone regeneration achieved through one-step alignment of human mesenchymal stem cell-embedded collagen","volume":"196","author":"Heo","year":"2025","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2025.11.013_b20","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5090\/aa8fb8","article-title":"Effect of scaffold morphology and cell co-culture on tenogenic differentiation of HADMSC on centrifugal melt electrospun poly (Llactic acid) fibrous meshes","volume":"9","author":"Wu","year":"2017","journal-title":"Biofabrication"},{"key":"10.1016\/j.actbio.2025.11.013_b21","doi-asserted-by":"crossref","DOI":"10.1016\/j.msec.2020.111787","article-title":"The effects of alignment and diameter of electrospun fibers on the cellular behaviors and osteogenesis of BMSCs","volume":"120","author":"Xie","year":"2021","journal-title":"Mater. Sci. Eng.: C"},{"key":"10.1016\/j.actbio.2025.11.013_b22","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1039\/C6IB00030D","article-title":"Local extracellular matrix alignment directs cellular protrusion dynamics and migration through Rac1 and FAK","volume":"8","author":"Carey","year":"2016","journal-title":"Integr. Biol. (United Kingdom)"},{"key":"10.1016\/j.actbio.2025.11.013_b23","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.actbio.2020.05.027","article-title":"Remodeling of aligned fibrous extracellular matrix by encapsulated cells under mechanical stretching","volume":"112","author":"Pei","year":"2020","journal-title":"Acta Biomater."},{"issue":"34","key":"10.1016\/j.actbio.2025.11.013_b24","doi-asserted-by":"crossref","first-page":"9031","DOI":"10.1016\/j.biomaterials.2010.08.021","article-title":"The effect of nanofiber-guided cell alignment on the preferential differentiation of neural stem cells","volume":"31","author":"Lim","year":"2010","journal-title":"Biomaterials"},{"issue":"1","key":"10.1016\/j.actbio.2025.11.013_b25","article-title":"Smooth muscle cell alignment and phenotype control by melt spun polycaprolactone fibers for seeding of tissue engineered blood vessels","volume":"2015","author":"Agrawal","year":"2015","journal-title":"Int. J. Biomater."},{"issue":"28","key":"10.1016\/j.actbio.2025.11.013_b26","doi-asserted-by":"crossref","first-page":"5728","DOI":"10.1039\/C8SM02605J","article-title":"Microextrusion printing cell-laden networks of type i collagen with patterned fiber alignment and geometry","volume":"15","author":"Nerger","year":"2019","journal-title":"Soft Matter"},{"key":"10.1016\/j.actbio.2025.11.013_b27","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.actbio.2016.12.041","article-title":"Fabrication of macromolecular gradients in aligned fiber scaffolds using a combination of in-line blending and air-gap electrospinning","volume":"56","author":"Kishan","year":"2017","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2025.11.013_b28","doi-asserted-by":"crossref","first-page":"8313","DOI":"10.1016\/j.biomaterials.2010.07.066","article-title":"Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal reconstruction and regeneration","volume":"31","author":"Builles","year":"2010","journal-title":"Biomaterials"},{"key":"10.1016\/j.actbio.2025.11.013_b29","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5090\/ac7824","article-title":"Local extensional flows promote long-range fiber alignment in 3D collagen hydrogels","volume":"14","author":"Ahmed","year":"2022","journal-title":"Biofabrication"},{"issue":"11","key":"10.1016\/j.actbio.2025.11.013_b30","doi-asserted-by":"crossref","first-page":"2546","DOI":"10.1016\/j.bpj.2014.10.035","article-title":"3D collagen alignment limits protrusions to enhance breast cancer cell persistence","volume":"107","author":"Riching","year":"2015","journal-title":"Biophys. J."},{"key":"10.1016\/j.actbio.2025.11.013_b31","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.202308071","article-title":"Microengineering 3D collagen matrices with tumor-mimetic gradients in fiber alignment","volume":"34","author":"Joshi","year":"2024","journal-title":"Adv. Funct. Mater."},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b32","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1007\/s10529-017-2494-z","article-title":"Aligned electrospun fibers for neural patterning","volume":"40","author":"Soliman","year":"2018","journal-title":"Biotechnol. Lett."},{"issue":"11","key":"10.1016\/j.actbio.2025.11.013_b33","doi-asserted-by":"crossref","first-page":"7710","DOI":"10.1021\/acsabm.4c01198","article-title":"Electrospinning Aligned SF\/Magnetic Nanoparticles-Blend Nanofiber Scaffolds for Inducing Skeletal Myoblast Alignment and Differentiation","volume":"7","author":"Yang","year":"2024","journal-title":"ACS Appl. Bio Mater."},{"issue":"10","key":"10.1016\/j.actbio.2025.11.013_b34","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1016\/S0008-8846(03)00108-X","article-title":"Fiber alignment and property direction dependency of FRC extrudate","volume":"33","author":"Qian","year":"2003","journal-title":"Cem. Concr. Res."},{"key":"10.1016\/j.actbio.2025.11.013_b35","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.actbio.2014.10.012","article-title":"Directing collagen fibers using counter-rotating cone extrusion","volume":"12","author":"Hoogenkamp","year":"2015","journal-title":"Acta Biomater."},{"issue":"8","key":"10.1016\/j.actbio.2025.11.013_b36","doi-asserted-by":"crossref","first-page":"2967","DOI":"10.1021\/acsbiomaterials.8b00384","article-title":"Collagen Fiber Orientation Regulates 3D Vascular Network Formation and Alignment","volume":"4","author":"McCoy","year":"2018","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"24","key":"10.1016\/j.actbio.2025.11.013_b37","doi-asserted-by":"crossref","first-page":"eaaz7748","DOI":"10.1126\/sciadv.aaz7748","article-title":"Marangoni flows drive the alignment of fibrillar cell-laden hydrogels","volume":"6","author":"Nerger","year":"2020","journal-title":"Sci. Adv."},{"issue":"9","key":"10.1016\/j.actbio.2025.11.013_b38","doi-asserted-by":"crossref","first-page":"eaaw2459","DOI":"10.1126\/sciadv.aaw2459","article-title":"Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels","volume":"5","author":"Skylar-Scott","year":"2019","journal-title":"Sci. Adv."},{"key":"10.1016\/j.actbio.2025.11.013_b39","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/s41563-020-00803-5","article-title":"Recapitulating macro-scale tissue self-organization through organoid bioprinting","volume":"20","author":"Brassard","year":"2021","journal-title":"Nat. Mater."},{"issue":"21\u201322","key":"10.1016\/j.actbio.2025.11.013_b40","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1089\/ten.tea.2017.0533","article-title":"Biofabricated Structures Reconstruct Functional Urinary Bladders in Radiation-Injured Rat Bladders","volume":"24","author":"Imamura","year":"2018","journal-title":"Tissue Eng. - Part A"},{"issue":"6452","key":"10.1016\/j.actbio.2025.11.013_b41","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1126\/science.aav9051","article-title":"3D bioprinting of collagen to rebuild components of the human heart","volume":"365","author":"Lee","year":"2019","journal-title":"Science"},{"issue":"17","key":"10.1016\/j.actbio.2025.11.013_b42","doi-asserted-by":"crossref","first-page":"eadu5905","DOI":"10.1126\/sciadv.adu5905","article-title":"3D bioprinting of collagen-based high-resolution internally perfusable scaffolds for engineering fully biologic tissue systems","volume":"11","author":"Shiwarski","year":"2025","journal-title":"Sci. Adv."},{"key":"10.1016\/j.actbio.2025.11.013_b43","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.3390\/bioengineering10091052","article-title":"Three-Dimensional Bioprinting of Human Organs and Tissues: Bioethical and Medico-Legal Implications Examined through a Scoping Review","volume":"10","author":"Ricci","year":"2023","journal-title":"Bioengineering"},{"key":"10.1016\/j.actbio.2025.11.013_b44","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1038\/s44222-024-00268-0","article-title":"Organoid bioprinting: from cells to functional tissues","volume":"3","author":"Huang","year":"2025","journal-title":"Nat. Rev. Bioeng."},{"key":"10.1016\/j.actbio.2025.11.013_b45","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1038\/nbt.2958","article-title":"3D bioprinting of tissues and organs","volume":"32","author":"Murphy","year":"2014","journal-title":"Nature Biotechnol."},{"issue":"5","key":"10.1016\/j.actbio.2025.11.013_b46","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/BF01412215","article-title":"Drawing and extrusion of semi-crystalline polymers","volume":"265","author":"Peterlin","year":"1987","journal-title":"Colloid Polym. Sci."},{"key":"10.1016\/j.actbio.2025.11.013_b47","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1007\/BF00541391","article-title":"Hydrostatic extrusion of solid polymers - Part 5 Structure and molecular orientation of extruded polyethylene","volume":"10","author":"Nakayama","year":"1975","journal-title":"J. Mater. Sci."},{"key":"10.1016\/j.actbio.2025.11.013_b48","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5090\/ab1d44","article-title":"Achieving molecular orientation in thermally extruded 3D printed objects","volume":"11","author":"Ghodbane","year":"2019","journal-title":"Biofabrication"},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b49","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s40964-022-00347-x","article-title":"Stress-adapted fiber orientation along the principal stress directions for continuous fiber-reinforced material extrusion","volume":"8","author":"Heitkamp","year":"2023","journal-title":"Prog. Addit. Manuf."},{"issue":"8","key":"10.1016\/j.actbio.2025.11.013_b50","doi-asserted-by":"crossref","first-page":"3606","DOI":"10.1002\/pen.26787","article-title":"Solid-state extrusion of nascent disentangled ultra-high molecular weight polyethylene","volume":"64","author":"Christakopoulos","year":"2024","journal-title":"Polym. Eng. Sci."},{"key":"10.1016\/j.actbio.2025.11.013_b51","doi-asserted-by":"crossref","first-page":"43401","DOI":"10.1038\/srep43401","article-title":"3D-Printing of Meso-structurally Ordered Carbon Fiber\/Polymer Composites with Unprecedented Orthotropic Physical Properties","volume":"7","author":"Lewicki","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.actbio.2025.11.013_b52","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.ohx.2018.02.001","article-title":"Large volume syringe pump extruder for desktop 3D printers","volume":"3","author":"Pusch","year":"2018","journal-title":"HardwareX"},{"issue":"13","key":"10.1016\/j.actbio.2025.11.013_b53","doi-asserted-by":"crossref","first-page":"eade7880","DOI":"10.1126\/sciadv.ade7880","article-title":"3D bioprinting of dynamic hydrogel bioinks enabled by small molecule modulators","volume":"9","author":"Hull","year":"2023","journal-title":"Sci. Adv."},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b54","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1167\/tvst.9.3.26","article-title":"Reproducible derivation and expansion of corneal mesenchymal stromal cells for therapeutic applications","volume":"9","author":"Jabbehdari","year":"2020","journal-title":"Transl. Vis. Sci. Technol."},{"issue":"1","key":"10.1016\/j.actbio.2025.11.013_b55","doi-asserted-by":"crossref","DOI":"10.1117\/1.JBO.19.1.016007","article-title":"Computational segmentation of collagen fibers from second-harmonic generation images of breast cancer","volume":"19","author":"Bredfeldt","year":"2014","journal-title":"J. Biomed. Opt."},{"issue":"37","key":"10.1016\/j.actbio.2025.11.013_b56","article-title":"Collagen-based bioinks for regenerative medicine: Fabrication, application and prospective","volume":"17","author":"Li","year":"2023","journal-title":"Med. Nov. Technol. Devices"},{"issue":"1","key":"10.1016\/j.actbio.2025.11.013_b57","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1021\/acsbiomaterials.4c01826","article-title":"FRESH 3D Bioprinting of Collagen Types I, II, and III","volume":"11","author":"Moss","year":"2025","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b58","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.jsb.2004.07.001","article-title":"Assembly of collagen into microribbons: Effects of pH and electrolytes","volume":"148","author":"Jiang","year":"2004","journal-title":"J. Struct. Biol."},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b59","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1002\/bdrc.20130","article-title":"Collagen fibrillogenesis in tendon development: Current models and regulation of fibril assembly","volume":"84","author":"Banos","year":"2008","journal-title":"Birth Defects Res. Part C - Embryo Today: Rev."},{"issue":"7","key":"10.1016\/j.actbio.2025.11.013_b60","doi-asserted-by":"crossref","first-page":"2330","DOI":"10.1021\/la3048104","article-title":"Type i collagen self-assembly: The roles of substrate and concentration","volume":"29","author":"Fang","year":"2013","journal-title":"Langmuir"},{"issue":"40","key":"10.1016\/j.actbio.2025.11.013_b61","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2209524119","article-title":"Discovering design principles of collagen molecular stability using a genetic algorithm, deep learning, and experimental validation","volume":"119","author":"Khare","year":"2022","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.actbio.2025.11.013_b62","doi-asserted-by":"crossref","DOI":"10.1016\/j.jnnfm.2024.105352","article-title":"Enhancing polymer fiber orientation with 3D-printed shell\u2013core structures","volume":"335","author":"Lou","year":"2025","journal-title":"J. Non-Newton. Fluid Mech."},{"issue":"25","key":"10.1016\/j.actbio.2025.11.013_b63","doi-asserted-by":"crossref","first-page":"eadw0808","DOI":"10.1126\/sciadv.adw0808","article-title":"Why are soft collagenous tissues so tough?","volume":"11","author":"Tang","year":"2025","journal-title":"Sci. Adv."},{"issue":"5","key":"10.1016\/j.actbio.2025.11.013_b64","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/j.joca.2021.01.009","article-title":"Quantitative three-dimensional collagen orientation analysis of human meniscus posterior horn in health and osteoarthritis using micro-computed tomography","volume":"29","author":"Karjalainen","year":"2021","journal-title":"Osteoarthr. Cartil."},{"issue":"7","key":"10.1016\/j.actbio.2025.11.013_b65","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1007\/s10439-012-0525-4","article-title":"Multi-scale structural and tensile mechanical response of annulus fibrosus to osmotic loading","volume":"40","author":"Han","year":"2012","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.actbio.2025.11.013_b66","article-title":"Extrusion width critically affects fibre orientation in short fibre reinforced material extrusion additive manufacturing","volume":"49","author":"Yan","year":"2022","journal-title":"Addit. Manuf."},{"key":"10.1016\/j.actbio.2025.11.013_b67","series-title":"Rheology: Principles, Measurements, and Applications","author":"Macosko","year":"1994"},{"key":"10.1016\/j.actbio.2025.11.013_b68","doi-asserted-by":"crossref","DOI":"10.1016\/j.compositesb.2023.110793","article-title":"Influence of printing conditions on the extrudate shape and fiber orientation in extrusion deposition additive manufacturing","volume":"261","author":"Pibulchinda","year":"2023","journal-title":"Compos. Part B: Eng."},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b69","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5090\/ac6bbe","article-title":"Engineered assistive materials for 3D bioprinting: support baths and sacrificial inks","volume":"14","author":"Brunel","year":"2022","journal-title":"Biofabrication"},{"key":"10.1016\/j.actbio.2025.11.013_b70","article-title":"Aqueous Two-Phase Enabled Low Viscosity 3D (LoV3D) Bioprinting of Living Matter","volume":"10","author":"Becker","year":"2023","journal-title":"Adv. Sci."},{"issue":"6","key":"10.1016\/j.actbio.2025.11.013_b71","doi-asserted-by":"crossref","first-page":"2589","DOI":"10.1021\/acsbiomaterials.2c00269","article-title":"One-step FRESH bioprinting of low-viscosity silk fibroin inks","volume":"8","author":"Sakai","year":"2022","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"8","key":"10.1016\/j.actbio.2025.11.013_b72","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.1500655","article-title":"Writing in the granular gel medium","volume":"1","author":"Bhattacharjee","year":"2015","journal-title":"Sci. Adv."},{"key":"10.1016\/j.actbio.2025.11.013_b73","article-title":"Embedded 3D bioprinting of collagen inks into mikrogel baths to control hydrogel microstructure and cell spreading","volume":"13","author":"Brunel","year":"2024","journal-title":"Adv. Heal. Mater."},{"issue":"28","key":"10.1016\/j.actbio.2025.11.013_b74","doi-asserted-by":"crossref","first-page":"32561","DOI":"10.1021\/acsami.2c08047","article-title":"Suppression of Filament Defects in Embedded 3D Printing","volume":"14","author":"Friedrich","year":"2022","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"10","key":"10.1016\/j.actbio.2025.11.013_b75","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1021\/acsbiomaterials.6b00184","article-title":"Stability of High Speed 3D Printing in Liquid-Like Solids","volume":"2","author":"Leblanc","year":"2016","journal-title":"ACS Biomater. Sci. Eng."},{"key":"10.1016\/j.actbio.2025.11.013_b76","series-title":"The Extracellular Matrix: An Overview","first-page":"77","article-title":"Collagens, suprastructures, and collagen fibril assembly","author":"Birk","year":"2011"},{"key":"10.1016\/j.actbio.2025.11.013_b77","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2021.644595","article-title":"Mimicking the Hierarchical Organization of Natural Collagen: Toward the Development of Ideal Scaffolding Material for Tissue Regeneration","volume":"9","author":"Salvatore","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"2","key":"10.1016\/j.actbio.2025.11.013_b78","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.jsb.2010.01.019","article-title":"Three-dimensional reconstruction of collagen-proteoglycan interactions in the mouse corneal stroma by electron tomography","volume":"170","author":"Parfitt","year":"2010","journal-title":"J. Struct. Biol."},{"issue":"266","key":"10.1016\/j.actbio.2025.11.013_b79","doi-asserted-by":"crossref","first-page":"266ra172","DOI":"10.1126\/scitranslmed.3009644","article-title":"Human limbal biopsy-derived stromal stem cells prevent corneal scarring","volume":"6","author":"Basu","year":"2014","journal-title":"Sci. Transl. Med."},{"issue":"5","key":"10.1016\/j.actbio.2025.11.013_b80","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1002\/stem.2781","article-title":"Cornea-Derived Mesenchymal Stromal Cells Therapeutically Modulate Macrophage Immunophenotype and Angiogenic Function","volume":"36","author":"Eslani","year":"2018","journal-title":"Stem Cells"},{"key":"10.1016\/j.actbio.2025.11.013_b81","first-page":"414","article-title":"In situ UNIversal Orthogonal Network (UNION) bioink deposition for direct delivery of corneal stromal stem cells to corneal wounds","volume":"48","author":"Brunel","year":"2025","journal-title":"Bioact. Mater."},{"key":"10.1016\/j.actbio.2025.11.013_b82","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1634\/stemcells.2004-0256","article-title":"Multipotent stem cells in human corneal stroma","volume":"23","author":"Du","year":"2005","journal-title":"Stem Cells"},{"issue":"5","key":"10.1016\/j.actbio.2025.11.013_b83","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.exer.2006.05.011","article-title":"Aldehyde dehydrogenase (ALDH) 3A1 expression by the human keratocyte and its repair phenotypes","volume":"83","author":"Pei","year":"2006","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.actbio.2025.11.013_b84","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/s43586-021-00073-8","article-title":"3D extrusion bioprinting","volume":"1","author":"Zhang","year":"2021","journal-title":"Nat. Rev. Methods Prim."},{"key":"10.1016\/j.actbio.2025.11.013_b85","first-page":"65","article-title":"Cellulose nanofibers and other biopolymers for biomedical applications. A review","volume":"10","author":"Moohan","year":"2020","journal-title":"Appl. Sci. (Switzerland)"},{"issue":"9","key":"10.1016\/j.actbio.2025.11.013_b86","doi-asserted-by":"crossref","first-page":"3690","DOI":"10.1021\/acsbiomaterials.2c00786","article-title":"Recent advances in designing fibrous biomaterials for the domain of biomedical, clinical, and environmental applications","volume":"8","author":"Mamidi","year":"2022","journal-title":"ACS Biomater. Sci. Eng."},{"key":"10.1016\/j.actbio.2025.11.013_b87","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.actbio.2025.01.009","article-title":"Interpenetrating networks of fibrillar and amorphous collagen promote cell spreading and hydrogel stability","volume":"193","author":"Brunel","year":"2025","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2025.11.013_b88","series-title":"Design Data for Reinforced Plastics","first-page":"96","article-title":"Mechanical properties of aligned fibre composites","author":"Hancox","year":"1994"},{"key":"10.1016\/j.actbio.2025.11.013_b89","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1002\/pc.750140503","article-title":"Fiber orientation and mechanical properties of short-fiber-reinforced injection-molded composites: Simulated and experimental results","volume":"14","author":"Gupta","year":"1993","journal-title":"Polym. Compos."},{"key":"10.1016\/j.actbio.2025.11.013_b90","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1021\/am404624z","article-title":"Cellulose nanowhiskers and fiber alignment greatly improve mechanical properties of electrospun prolamin protein fibers","volume":"6","author":"Wang","year":"2014","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.actbio.2025.11.013_b91","article-title":"Effect of fibre alignment on mechanical properties of natural fibre reinforced polymer composites","volume":"vol. 912","author":"Kamaraj","year":"2020"},{"key":"10.1016\/j.actbio.2025.11.013_b92","doi-asserted-by":"crossref","DOI":"10.1016\/j.compositesb.2021.109467","article-title":"Highly aligned aramid nanofibrillar nanocomposites for enhanced dynamic mechanical properties","volume":"229","author":"Lee","year":"2022","journal-title":"Compos. Part B: Eng."},{"key":"10.1016\/j.actbio.2025.11.013_b93","doi-asserted-by":"crossref","first-page":"7885","DOI":"10.1016\/j.matpr.2020.12.614","article-title":"Study on the effects of fiber orientation on the mechanical properties of natural fiber reinforced epoxy composite by finite element method","volume":"45","author":"Gupta","year":"2021","journal-title":"Mater. Today Proc."},{"key":"10.1016\/j.actbio.2025.11.013_b94","article-title":"Multi-layered hydrogels for biomedical applications","volume":"6","author":"Liu","year":"2018","journal-title":"Front. Chem."},{"key":"10.1016\/j.actbio.2025.11.013_b95","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.matt.2021.11.018","article-title":"Continuously growing multi-layered hydrogel structures with seamless interlocked interface","volume":"5","author":"Xu","year":"2022","journal-title":"Matter"},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b96","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1016\/j.actbio.2010.10.016","article-title":"Biomaterial guides for lymphatic endothelial cell alignment and migration","volume":"7","author":"Bouta","year":"2011","journal-title":"Acta Biomater."},{"issue":"2","key":"10.1016\/j.actbio.2025.11.013_b97","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1002\/bit.22697","article-title":"Modulation of alignment and differentiation of skeletal myoblasts by submicron ridges\/grooves surface structure","volume":"106","author":"Wang","year":"2010","journal-title":"Biotechnol. Bioeng."},{"issue":"3","key":"10.1016\/j.actbio.2025.11.013_b98","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1002\/bit.22268","article-title":"Alignment of skeletal muscle myoblasts and myotubes using linear micropatterned surfaces ground with abrasives","volume":"103","author":"Shimizu","year":"2009","journal-title":"Biotechnol. Bioeng."},{"issue":"9","key":"10.1016\/j.actbio.2025.11.013_b99","doi-asserted-by":"crossref","DOI":"10.3390\/polym12091986","article-title":"3D printing of a reactive hydrogel bio-ink using a static mixing tool","volume":"12","author":"Puertas-Bartolom\u00e9","year":"2020","journal-title":"Polymers"},{"issue":"8","key":"10.1016\/j.actbio.2025.11.013_b100","doi-asserted-by":"crossref","DOI":"10.3390\/ma18081765","article-title":"Influence of print speed on the mechanical performance of 3D-printed bio-polymer polylactic acid","volume":"18","author":"Lorkowski","year":"2025","journal-title":"Materials"},{"issue":"15","key":"10.1016\/j.actbio.2025.11.013_b101","doi-asserted-by":"crossref","DOI":"10.3390\/polym17152090","article-title":"The impact of elevated printing speeds and filament color on the dimensional precision and tensile properties of FDM-printed PLA specimens","volume":"17","author":"Ardeljan","year":"2025","journal-title":"Polymers"},{"key":"10.1016\/j.actbio.2025.11.013_b102","doi-asserted-by":"crossref","first-page":"4404","DOI":"10.1021\/acs.biomac.5c00398","article-title":"Reinforcement of fibrillar collagen hydrogels with bioorthogonal covalent crosslinks","volume":"26","author":"Brunel","year":"2025","journal-title":"Biomacromolecules"}],"container-title":["Acta Biomaterialia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1742706125008360?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1742706125008360?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T08:55:18Z","timestamp":1793264118000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1742706125008360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":102,"alternative-id":["S1742706125008360"],"URL":"https:\/\/doi.org\/10.1016\/j.actbio.2025.11.013","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.05.20.654730","asserted-by":"object"}]},"ISSN":["1742-7061"],"issn-type":[{"value":"1742-7061","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dual-orientation of collagen fibers to guide cell alignment in 3D-printed constructs","name":"articletitle","label":"Article Title"},{"value":"Acta Biomaterialia","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actbio.2025.11.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Acta Materialia Inc. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}