{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:54:41Z","timestamp":1790891681052,"version":"4.1.0"},"reference-count":95,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003134","name":"Fund for Research and Training in Industry and Agriculture","doi-asserted-by":"publisher","award":["FC 47333"],"award-info":[{"award-number":["FC 47333"]}],"id":[{"id":"10.13039\/501100003134","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007146","name":"Allgemeine Unfallversicherungsanstalt","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007146","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002661","name":"Fund for Scientific Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Biomaterialia"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.actbio.2026.07.048","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:19:55Z","timestamp":1785424795000},"page":"353-365","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Aging modifies microstructure and material properties of mineralized cartilage and subchondral bone in the murine knee"],"prefix":"10.1016","volume":"222","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0383-4257","authenticated-orcid":false,"given":"Laura","family":"M\u00fcller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6575-8443","authenticated-orcid":false,"given":"St\u00e9phane","family":"Blouin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1675-6309","authenticated-orcid":false,"given":"Edoardo","family":"Pedrinazzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8303-4959","authenticated-orcid":false,"given":"G. Harry","family":"van Lenthe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7968-6795","authenticated-orcid":false,"given":"Alexandre","family":"Hego","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Weinkamer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6046-0365","authenticated-orcid":false,"given":"Markus A.","family":"Hartmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3714-7594","authenticated-orcid":false,"given":"Davide","family":"Ruffoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.actbio.2026.07.048_bib0001","doi-asserted-by":"crossref","first-page":"085","DOI":"10.1055\/s-0032-1319782","article-title":"The cartilage-bone interface","volume":"25","author":"Hoemann","year":"2012","journal-title":"J. Knee Surg."},{"key":"10.1016\/j.actbio.2026.07.048_bib0002","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.659654","article-title":"Macro, micro, and molecular. Changes of the osteochondral interface in osteoarthritis development","volume":"9","author":"Fan","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.actbio.2026.07.048_bib0003","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1089\/ten.teb.2018.0122","article-title":"Beyond cartilage repair: the role of the osteochondral unit in joint health and disease","volume":"25","author":"Lepage","year":"2019","journal-title":"Tissue Eng. B Rev."},{"key":"10.1016\/j.actbio.2026.07.048_bib0004","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202001008","article-title":"Innovative tissue-engineered strategies for osteochondral defect repair and regeneration: current progress and challenges","volume":"9","author":"Zhou","year":"2020","journal-title":"Adv. Healthcare Mater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0005","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/S1369-7021(11)70056-6","article-title":"Artful interfaces within biological materials","volume":"14","author":"Dunlop","year":"2011","journal-title":"Mater. Today"},{"key":"10.1016\/j.actbio.2026.07.048_bib0006","series-title":"Structural Interfaces and Attachments in Biology","author":"Thomopoulos","year":"2012"},{"key":"10.1016\/j.actbio.2026.07.048_bib0007","doi-asserted-by":"crossref","first-page":"11702","DOI":"10.1021\/acs.nanolett.3c03564","article-title":"Understanding the mechanics of the temporomandibular joint osteochondral interface from micro- and nanoscopic perspectives","volume":"23","author":"Su","year":"2023","journal-title":"Nano Lett."},{"key":"10.1016\/j.actbio.2026.07.048_bib0008","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202407649","article-title":"Nonlinear stress-induced transformations in collagen fibrillar organization, disorder and strain mechanisms in the bone-cartilage unit","volume":"12","author":"Badar","year":"2025","journal-title":"Adv. Sci."},{"key":"10.1016\/j.actbio.2026.07.048_bib0009","doi-asserted-by":"crossref","first-page":"4389","DOI":"10.1016\/j.actbio.2012.07.042","article-title":"Nanomechanical mapping of the osteochondral interface with contact resonance force microscopy and nanoindentation","volume":"8","author":"Campbell","year":"2012","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0010","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.1021\/acs.nanolett.1c04649","article-title":"Identification of an ultrathin osteochondral interface tissue with specific nanostructure at the Human knee joint","volume":"22","author":"Wang","year":"2022","journal-title":"Nano Lett."},{"key":"10.1016\/j.actbio.2026.07.048_bib0011","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/s43246-025-00972-3","article-title":"3D full-field lacunar morphology and deformation of calcified fibrocartilage in the loaded Achilles enthesis of a mouse","volume":"6","author":"Moayedi","year":"2025","journal-title":"Commun. Mater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0012","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.actbio.2023.04.018","article-title":"Structural and functional heterogeneity of mineralized fibrocartilage at the Achilles tendon-bone insertion","volume":"166","author":"Tits","year":"2023","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0013","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-95917-4","article-title":"Local anisotropy in mineralized fibrocartilage and subchondral bone beneath the tendon-bone interface","volume":"11","author":"Tits","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.actbio.2026.07.048_bib0014","article-title":"Joining soft tissues to bone: insights from modeling and simulations","volume":"14","author":"Tits","year":"2021","journal-title":"Bone Rep."},{"key":"10.1016\/j.actbio.2026.07.048_bib0015","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1111\/joa.13728","article-title":"Structural clues to articular calcified cartilage function: a descriptive review of this crucial interface tissue","volume":"241","author":"Evans","year":"2022","journal-title":"J. Anat."},{"key":"10.1016\/j.actbio.2026.07.048_bib0016","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/s41551-019-0477-1","article-title":"In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography","volume":"4","author":"Madi","year":"2020","journal-title":"Nat. Biomed. Eng."},{"key":"10.1016\/j.actbio.2026.07.048_bib0017","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1002\/jor.20883","article-title":"In situ measurement of transport between subchondral bone and articular cartilage","volume":"27","author":"Pan","year":"2009","journal-title":"J. Orthop. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0018","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/S1047-8477(02)00635-4","article-title":"Characteristics of mineral particles in the human bone\/cartilage interface","volume":"141","author":"Zizak","year":"2003","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.actbio.2026.07.048_bib0019","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.1002\/jbmr.4642","article-title":"Mineral crystal thickness in calcified cartilage and subchondral bone in healthy and osteoarthritic Human knees","volume":"37","author":"Finnil\u00e4","year":"2020","journal-title":"J. Bone Miner. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0020","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.jsb.2004.10.010","article-title":"Two different correlations between nanoindentation modulus and mineral content in the bone-cartilage interface","volume":"149","author":"Gupta","year":"2005","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.actbio.2026.07.048_bib0021","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.jsb.2009.08.010","article-title":"Histomorphometric analysis of adult articular calcified cartilage zone","volume":"168","author":"Wang","year":"2009","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.actbio.2026.07.048_bib0022","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1046\/j.1469-7580.2003.00193.x","article-title":"Nanomechanical properties and mineral concentration in articular calcified cartilage and subchondral bone","volume":"203","author":"Ferguson","year":"2003","journal-title":"J. Anat."},{"key":"10.1016\/j.actbio.2026.07.048_bib0023","doi-asserted-by":"crossref","first-page":"242","DOI":"10.22203\/eCM.v019a23","article-title":"Combined nanoindentation testing and scanning electron microscopy of bone and articular calcified cartilage in an equine fracture predilection site","volume":"19","author":"Doube","year":"2010","journal-title":"Eur. Cell Mater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0024","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.actbio.2025.01.047","article-title":"Zonal characteristics of collagen ultrastructure and responses to mechanical loading in articular cartilage","volume":"195","author":"Hu","year":"2025","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0025","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1302\/2046-3758.148.BJR-2024-0396.R1","article-title":"Type-I and -II collagens from bone and cartilage colocalize at the osteochondral cement line","volume":"14","author":"Hayat","year":"2025","journal-title":"Bone Jt. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0026","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/joa.12756","article-title":"Morphological, biochemical and mechanical properties of articular cartilage and subchondral bone in rat tibial plateau are age related","volume":"232","author":"Ren","year":"2018","journal-title":"J. Anat."},{"key":"10.1016\/j.actbio.2026.07.048_bib0027","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1016\/j.joca.2007.04.003","article-title":"Variations in articular calcified cartilage by site and exercise in the 18-month-old equine distal metacarpal condyle","volume":"15","author":"Doube","year":"2007","journal-title":"OsteoArthritis Cartilage"},{"key":"10.1016\/j.actbio.2026.07.048_bib0028","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.bone.2007.10.021","article-title":"Bone mineralization density distribution in health and disease","volume":"42","author":"Roschger","year":"2008","journal-title":"Bone"},{"key":"10.1016\/j.actbio.2026.07.048_bib0029","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1016\/j.bone.2007.01.012","article-title":"The bone mineralization density distribution as a fingerprint of the mineralization process","volume":"40","author":"Ruffoni","year":"2007","journal-title":"Bone"},{"key":"10.1016\/j.actbio.2026.07.048_bib0030","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s00223-021-00866-9","article-title":"The bone cartilage interface and osteoarthritis","volume":"109","author":"Boyde","year":"2021","journal-title":"Calcif. Tissue Int."},{"key":"10.1016\/j.actbio.2026.07.048_bib0031","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.actbio.2025.06.002","article-title":"Gradients in lacunar morphology and cartilage mineralization reflect the mechanical function of the mouse femoral head epiphysis","volume":"201","author":"Tang","year":"2025","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0032","article-title":"Comparative anatomy and morphology of the knee in translational models for articular cartilage disorders. Part II: small animals","volume":"234","author":"Ol\u00e1h","year":"2021","journal-title":"Ann. Anat. - Anat. Anz."},{"key":"10.1016\/j.actbio.2026.07.048_bib0033","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1002\/jor.23824","article-title":"Preclinical models for orthopedic research and bone tissue engineering","volume":"36","author":"Schindeler","year":"2018","journal-title":"J. Orthop. Res. Off. Publ. Orthop. Res. Soc."},{"key":"10.1016\/j.actbio.2026.07.048_bib0034","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1302\/2046-3758.34.2000238","article-title":"Animal models of cartilage repair","volume":"3","author":"Cook","year":"2014","journal-title":"Bone Jt. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0035","first-page":"1431","article-title":"The laboratory rat: age and body weight matter","volume":"20","author":"Ghasemi","year":"2021","journal-title":"EXCLI. J."},{"key":"10.1016\/j.actbio.2026.07.048_bib0036","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1177\/002215540305100312","article-title":"Temporal analysis of rat growth plates: cessation of growth with age despite presence of a physis","volume":"51","author":"Roach","year":"2003","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.actbio.2026.07.048_bib0037","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1002\/jbmr.141","article-title":"Guidelines for assessment of bone microstructure in rodents using micro-computed tomography","volume":"25","author":"Bouxsein","year":"2010","journal-title":"J. Bone Miner. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0038","doi-asserted-by":"crossref","DOI":"10.1016\/j.bone.2019.115043","article-title":"Effects of ex vivo ionizing radiation on collagen structure and whole-bone mechanical properties of mouse vertebrae","volume":"128","author":"Pendleton","year":"2019","journal-title":"Bone"},{"key":"10.1016\/j.actbio.2026.07.048_bib0039","article-title":"Influence of X-rays and gamma-rays on the mechanical performance of human bone factoring out intraindividual bone structure and composition indices","volume":"13","author":"Schmidt","year":"2022","journal-title":"Mater. Today Bio."},{"key":"10.1016\/j.actbio.2026.07.048_bib0040","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1109\/TSMC.1979.4310076","article-title":"A threshold selection method from gray-level histograms","volume":"9","author":"Otsu","year":"1979","journal-title":"IEEE Trans. Syst. Man Cybern."},{"key":"10.1016\/j.actbio.2026.07.048_bib0041","doi-asserted-by":"crossref","DOI":"10.1515\/jib-2017-0015","article-title":"Comparison of different approaches for measuring tibial cartilage thickness","volume":"14","author":"Maier","year":"2017","journal-title":"J. Integr. Bioinforma."},{"key":"10.1016\/j.actbio.2026.07.048_bib0042","doi-asserted-by":"crossref","DOI":"10.1002\/adma.202206110","article-title":"Curvature in biological systems: its quantification, emergence, and implications across the scales","volume":"35","author":"Schamberger","year":"2023","journal-title":"Adv. Mater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0043","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1046\/j.1365-2818.1997.1340694.x","article-title":"A new method for the model-independent assessment of thickness in three-dimensional images","volume":"185","author":"Hildebrand","year":"1997","journal-title":"J. Microsc."},{"key":"10.1016\/j.actbio.2026.07.048_bib0044","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S8756-3282(98)00112-4","article-title":"Validation of quantitative backscattered electron imaging for the measurement of mineral density distribution in human bone biopsies","volume":"23","author":"Roschger","year":"1998","journal-title":"Bone"},{"key":"10.1016\/j.actbio.2026.07.048_bib0045","first-page":"361","article-title":"Formation of ultracracks in methacrylate-embedded undecalcified bone samples by exposure to aqueous solutions","volume":"3","author":"Roschger","year":"1993","journal-title":"Cells Mater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0046","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1007\/s00223-021-00832-5","article-title":"Quantitative backscattered electron imaging of bone using a thermionic or a field emission electron source","volume":"109","author":"Hartmann","year":"2021","journal-title":"Calcif. Tissue Int."},{"key":"10.1016\/j.actbio.2026.07.048_bib0047","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1007\/s10955-011-0209-8","article-title":"The heterogeneous mineral content of bone\u2014using stochastic arguments and simulations to overcome experimental limitations","volume":"144","author":"Lukas","year":"2011","journal-title":"J. Stat. Phys."},{"key":"10.1016\/j.actbio.2026.07.048_bib0048","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202100113","article-title":"3D Interrelationship between osteocyte network and forming mineral during Human bone remodeling","volume":"10","author":"Ayoubi","year":"2021","journal-title":"Adv. Healthcare Mater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0049","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.1557\/jmr.2006.0259","article-title":"Quasistatic and dynamic nanomechanical properties of cancellous bone tissue relate to collagen content and organization","volume":"21","author":"Donnelly","year":"2006","journal-title":"J. Mater. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0050","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1002\/jbm.a.30633","article-title":"Effects of surface roughness and maximum load on the mechanical properties of cancellous bone measured by nanoindentation","volume":"77A","author":"Donnelly","year":"2006","journal-title":"J. Biomed. Mater. Res., Part A"},{"key":"10.1016\/j.actbio.2026.07.048_bib0051","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1557\/s43578-022-00541-1","article-title":"Accurate measurement of thin film mechanical properties using nanoindentation","volume":"37","author":"Zak","year":"2022","journal-title":"J. Mater. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0052","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1016\/j.jmps.2013.05.007","article-title":"Oliver\u2013Pharr indentation method in determining elastic moduli of shape memory alloys\u2014A phase transformable material","volume":"61","author":"Kan","year":"2013","journal-title":"J. Mech. Phys. Solids."},{"key":"10.1016\/j.actbio.2026.07.048_bib0053","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1529\/biophysj.104.047308","article-title":"Interpreting second-harmonic generation images of collagen I fibrils","volume":"88","author":"Williams","year":"2005","journal-title":"Biophys. J."},{"key":"10.1016\/j.actbio.2026.07.048_bib0054","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1038\/nprot.2012.009","article-title":"Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure","volume":"7","author":"Chen","year":"2012","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.actbio.2026.07.048_bib0055","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1002\/jbio.201500150","article-title":"Analysis of forward and backward Second Harmonic Generation images to probe the nanoscale structure of collagen within bone and cartilage","volume":"8","author":"Houle","year":"2015","journal-title":"J. Biophotonics."},{"key":"10.1016\/j.actbio.2026.07.048_bib0056","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.jsb.2013.04.010","article-title":"Unremodeled endochondral bone is a major architectural component of the cortical bone of the rat (Rattus norvegicus)","volume":"183","author":"Shipov","year":"2013","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.actbio.2026.07.048_bib0057","first-page":"923","article-title":"Age-related changes in the 3D hierarchical structure of rat tibia cortical bone characterized by high-resolution micro-CT","volume":"114","author":"Jast","year":"2013","journal-title":"J. Appl. Physiol. Bethesda Md"},{"key":"10.1016\/j.actbio.2026.07.048_bib0058","first-page":"278","article-title":"Topographical variations in articular cartilage and subchondral bone of the normal rat knee are age-related","volume":"196","author":"Hamann","year":"2014","journal-title":"Ann. Anat. Anat. Anz. Off. Organ Anat. Ges."},{"key":"10.1016\/j.actbio.2026.07.048_bib0059","doi-asserted-by":"crossref","DOI":"10.1016\/j.bone.2019.115037","article-title":"Age-related histological changes in calcified cartilage and subchondral bone in femoral heads from healthy humans","volume":"129","author":"Nielsen","year":"2019","journal-title":"Bone"},{"key":"10.1016\/j.actbio.2026.07.048_bib0060","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.bone.2018.12.003","article-title":"Spatially matching morphometric assessment of cartilage and subchondral bone in osteoarthritic human knee joint with micro-computed tomography","volume":"120","author":"Gatenholm","year":"2019","journal-title":"Bone"},{"key":"10.1016\/j.actbio.2026.07.048_bib0061","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1097\/00002281-199805000-00017","article-title":"The importance of subchondral bone in osteoarthrosis","volume":"10","author":"Burr","year":"1998","journal-title":"Curr. Opin. Rheumatol."},{"key":"10.1016\/j.actbio.2026.07.048_bib0062","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1159\/000213866","article-title":"Do subchondral bone changes exacerbate or precede articular cartilage destruction in osteoarthritis of the elderly?","volume":"43","author":"Bailey","year":"2009","journal-title":"Gerontology"},{"key":"10.1016\/j.actbio.2026.07.048_bib0063","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1002\/jor.23459","article-title":"BMI-related microstructural changes in the tibial subchondral trabecular bone of patients with knee osteoarthritis","volume":"35","author":"Reina","year":"2017","journal-title":"J. Orthop. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0064","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.bone.2015.03.022","article-title":"Multi-level femoral morphology and mechanical properties of rats of different ages","volume":"76","author":"Zhang","year":"2015","journal-title":"Bone"},{"key":"10.1016\/j.actbio.2026.07.048_bib0065","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1359\/jbmr.080711","article-title":"Effect of temporal changes in bone turnover on the bone mineralization density distribution: a computer simulation study","volume":"23","author":"Ruffoni","year":"2008","journal-title":"J. Bone Miner. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0066","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1002\/(SICI)1097-0185(199609)246:1<39::AID-AR5>3.0.CO;2-A","article-title":"Trabecular and endocortical bone surfaces in the rat: modeling or remodeling?","volume":"246","author":"Erben","year":"1996","journal-title":"Anat. Rec."},{"key":"10.1016\/j.actbio.2026.07.048_bib0067","doi-asserted-by":"crossref","DOI":"10.1016\/j.tice.2022.101993","article-title":"The chondro-osseous junction of articular cartilage","volume":"80","author":"Zecca","year":"2023","journal-title":"Tissue Cell"},{"key":"10.1016\/j.actbio.2026.07.048_bib0068","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.actbio.2024.11.015","article-title":"Endochondral ossification: insights into the cartilage mineralization processes achieved by an anhydrous freeze substitution protocol","volume":"191","author":"Boonrungsiman","year":"2025","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0069","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioadv.2025.214320","article-title":"Understanding the structure and mechanics of the sheep calcaneal enthesis: a relevant animal model to design scaffolds for tissue engineering applications","volume":"175","author":"Sensini","year":"2025","journal-title":"Biomater. Adv."},{"key":"10.1016\/j.actbio.2026.07.048_bib0070","doi-asserted-by":"crossref","DOI":"10.1002\/anbr.202100162","article-title":"A 3D network of nanochannels for possible ion and molecule transit in mineralizing bone and cartilage","volume":"2","author":"Tang","year":"2022","journal-title":"Adv. Nanobiomed. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0071","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-47545-2","article-title":"Concentric lamellae \u2013 novel microanatomical structures in the articular calcified cartilage of mice","volume":"9","author":"Keenan","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.actbio.2026.07.048_bib0072","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s10522-006-9076-0","article-title":"Variation in articular cartilage in rats between 3 and 32 months old. A histomorphometric and scanning electron microscopy study","volume":"8","author":"Oda","year":"2007","journal-title":"Biogerontology"},{"key":"10.1016\/j.actbio.2026.07.048_bib0073","doi-asserted-by":"crossref","first-page":"R70","DOI":"10.1530\/EC-19-0531","article-title":"Phenomenon of osteocyte lacunar mineralization: indicator of former osteocyte death and a novel marker of impaired bone quality?","volume":"9","author":"Milovanovic","year":"2020","journal-title":"Endocr. Connect."},{"key":"10.1016\/j.actbio.2026.07.048_bib0074","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.actbio.2022.11.051","article-title":"Osteocyte lacunae in transiliac bone biopsy samples across life span","volume":"157","author":"Blouin","year":"2023","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0075","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s10856-018-6066-0","article-title":"Zone-dependent mechanical properties of human articular cartilage obtained by indentation measurements","volume":"29","author":"Antons","year":"2018","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"10.1016\/j.actbio.2026.07.048_bib0076","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.msec.2010.12.005","article-title":"Dynamic nanoindentation of articular porcine cartilage","volume":"31","author":"Franke","year":"2011","journal-title":"Mater. Sci. Eng. C"},{"key":"10.1016\/j.actbio.2026.07.048_bib0077","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1186\/1471-2474-15-205","article-title":"Viscoelastic properties of bovine knee joint articular cartilage: dependency on thickness and loading frequency","volume":"15","author":"Espino","year":"2014","journal-title":"BMC. Musculoskelet. Disord."},{"key":"10.1016\/j.actbio.2026.07.048_bib0078","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3390\/jfb11040085","article-title":"Hardness, an important indicator of bone quality, and the role of collagen in bone Hardness","volume":"11","author":"Ibrahim","year":"2020","journal-title":"J. Funct. Biomater."},{"issue":"527","key":"10.1016\/j.actbio.2026.07.048_bib0079","article-title":"On wear mechanisms and microstructural changes in nano-scratches of fcc metals","volume":"526-527","author":"Rojacz","year":"2023","journal-title":"Wear"},{"key":"10.1016\/j.actbio.2026.07.048_bib0080","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1038\/s41514-024-00156-2","article-title":"Multiscale and multidisciplinary analysis of aging processes in bone","volume":"10","author":"Ravazzano","year":"2024","journal-title":"NPJ. Aging"},{"key":"10.1016\/j.actbio.2026.07.048_bib0081","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1002\/jor.20337","article-title":"Age-related factors affecting the postyield energy dissipation of human cortical bone","volume":"25","author":"Nyman","year":"2007","journal-title":"J. Orthop. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0082","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1111\/j.1469-7580.2012.01479.x","article-title":"A multi-modal multiphoton investigation of microstructure in the deep zone and calcified cartilage","volume":"220","author":"Mansfield","year":"2012","journal-title":"J. Anat."},{"key":"10.1016\/j.actbio.2026.07.048_bib0083","doi-asserted-by":"crossref","first-page":"2826","DOI":"10.1038\/s41598-018-21005-9","article-title":"Quantitative morphometry for osteochondral tissues using second harmonic generation microscopy and image texture information","volume":"8","author":"Saitou","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.actbio.2026.07.048_bib0084","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.actbio.2025.01.047","article-title":"Zonal characteristics of collagen ultrastructure and responses to mechanical loading in articular cartilage","volume":"195","author":"Hu","year":"2025","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0085","article-title":"Cement lines are stiffer and harder than bone but exhibit different mineral\u2013Mechanics relationships due to thicker and shorter mineral particles","volume":"7","author":"Cantamessa","year":"2026","journal-title":"Small Struct."},{"key":"10.1016\/j.actbio.2026.07.048_bib0086","doi-asserted-by":"crossref","DOI":"10.1093\/jbmrpl\/ziaf114","article-title":"The mineralization of osteonal cement line depends on where the osteon is formed","volume":"9","author":"Cantamessa","year":"2025","journal-title":"JBMR. Plus."},{"key":"10.1016\/j.actbio.2026.07.048_bib0087","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.actbio.2020.11.002","article-title":"Focused ion beam-SEM 3D analysis of mineralized osteonal bone: lamellae and cement sheath structures","volume":"121","author":"Raguin","year":"2021","journal-title":"Acta Biomater."},{"key":"10.1016\/j.actbio.2026.07.048_bib0088","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.jsb.2010.11.014","article-title":"The organization of the osteocyte network mirrors the extracellular matrix orientation in bone","volume":"173","author":"Kerschnitzki","year":"2011","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.actbio.2026.07.048_bib0089","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40634-015-0037-x","article-title":"The benefits and limitations of animal models for translational research in cartilage repair","volume":"3","author":"Moran","year":"2016","journal-title":"J. Exp. Orthop."},{"key":"10.1016\/j.actbio.2026.07.048_bib0090","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1089\/ten.teb.2009.0452","article-title":"Animal models for cartilage regeneration and repair","volume":"16","author":"Chu","year":"2010","journal-title":"Tissue Eng. B Rev."},{"key":"10.1016\/j.actbio.2026.07.048_bib0091","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1007\/s00223-024-01289-y","article-title":"The lacunocanalicular network is denser in C57BL\/6 compared to BALB\/c mice","volume":"115","author":"Rummler","year":"2024","journal-title":"Calcif. Tissue Int."},{"key":"10.1016\/j.actbio.2026.07.048_bib0092","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1007\/s00223-015-9977-5","article-title":"The role of water compartments in the material properties of cortical bone","volume":"97","author":"Granke","year":"2015","journal-title":"Calcif. Tissue Int."},{"key":"10.1016\/j.actbio.2026.07.048_bib0093","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1557\/jmr.2004.19.1.249","article-title":"Nanoindentation of bone: comparison of specimens tested in liquid and embedded in polymethylmethacrylate","volume":"19","author":"Bushby","year":"2004","journal-title":"J. Mater. Res."},{"key":"10.1016\/j.actbio.2026.07.048_bib0094","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.jbiomech.2013.11.022","article-title":"The effect of hydration on mechanical anisotropy, topography and fibril organization of the osteonal lamellae","volume":"47","author":"Faingold","year":"2014","journal-title":"J. Biomech."},{"key":"10.1016\/j.actbio.2026.07.048_bib0095","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/j.jbiomech.2005.01.012","article-title":"The influence of water removal on the strength and toughness of cortical bone","volume":"39","author":"Nyman","year":"2006","journal-title":"J. Biomech."}],"container-title":["Acta Biomaterialia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1742706126004988?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1742706126004988?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:58:21Z","timestamp":1790888301000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1742706126004988"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":95,"alternative-id":["S1742706126004988"],"URL":"https:\/\/doi.org\/10.1016\/j.actbio.2026.07.048","relation":{},"ISSN":["1742-7061"],"issn-type":[{"value":"1742-7061","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Aging modifies microstructure and material properties of mineralized cartilage and subchondral bone in the murine knee","name":"articletitle","label":"Article Title"},{"value":"Acta Biomaterialia","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.actbio.2026.07.048","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Acta Materialia Inc. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}