{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:52:10Z","timestamp":1791067930667,"version":"4.1.0"},"reference-count":18,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003810","name":"Hospital de Cl\u00ednicas de Porto Alegre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003810","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordination of Higher Education Personnel Improvement","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Histochemica"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.acthis.2026.152375","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:42:01Z","timestamp":1790419321000},"page":"152375","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of decalcification with 10% nitric acid solution and EDTA on Safranin O staining and aggrecan immunohistochemistry"],"prefix":"10.1016","volume":"128","author":[{"given":"Eduarda Correa","family":"Freitas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Visioli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiza Falc\u00e3o","family":"Vanzella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diorlon Nunes","family":"Machado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexia Antunes","family":"Deluca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Henrique Baroni","family":"Zortea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily Ferreira Salles","family":"Pilar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.acthis.2026.152375_bib1","series-title":"Handbook of histology methods for bone and cartilage","year":"2003"},{"issue":"9","key":"10.1016\/j.acthis.2026.152375_bib2","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1369\/0022155415591599","article-title":"The bone-inflammation-cartilage (BIC) stain: a novel staining method combining Safranin O and Van Gieson\u2019s stains","volume":"63","author":"Bergmann","year":"2015","journal-title":"J. Histochem. Cytochem."},{"issue":"8","key":"10.1016\/j.acthis.2026.152375_bib3","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1369\/0022155419850099","article-title":"Tissue morphology and antigenicity in mouse and rat tibia: comparing 12 different decalcification conditions","volume":"67","author":"Bogoevski","year":"2019","journal-title":"J. Histochem. Cytochem."},{"issue":"2","key":"10.1016\/j.acthis.2026.152375_bib4","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/BF00489922","article-title":"Limitations of safranin \u201cO\u201d staining in proteoglycan-depleted cartilage demonstrated with monoclonal antibodies","volume":"89","author":"Camplejohn","year":"1988","journal-title":"Histochemistry"},{"issue":"4","key":"10.1016\/j.acthis.2026.152375_bib5","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1302\/2046-3758.34.2000238","article-title":"Animal models of cartilage repair","volume":"3","author":"Cook","year":"2014","journal-title":"Bone Jt. Res."},{"key":"10.1016\/j.acthis.2026.152375_bib6","first-page":"1","article-title":"Evaluation of EDTA and nitric acid solutions for decalcification of joints in AG\/WT, BALB\/c, C57, DBA1\/J mice, and in Wistar rats","author":"Freitas","year":"2021","journal-title":"Biotech. Histochem."},{"key":"10.1016\/j.acthis.2026.152375_bib7","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/764621","article-title":"A review of translational animal models for knee osteoarthritis","volume":"2012","author":"Gregory","year":"2012","journal-title":"Arthritis"},{"issue":"6","key":"10.1016\/j.acthis.2026.152375_bib8","doi-asserted-by":"crossref","first-page":"264","DOI":"10.3109\/10520290009085130","article-title":"Safranin O staining using a microwave oven","volume":"75","author":"Kahveci","year":"2000","journal-title":"Biotech. Histochem."},{"issue":"4","key":"10.1016\/j.acthis.2026.152375_bib9","doi-asserted-by":"crossref","first-page":"818S","DOI":"10.1177\/1947603520932199","article-title":"Altered expression of aggrecan, FAM20B, B3GALT6, and EXTL2 in patients with osteoarthritis and Kashin-Beck disease","volume":"12","author":"Lei","year":"2021","journal-title":"Cartilage"},{"key":"10.1016\/j.acthis.2026.152375_bib10","article-title":"Evaluation of decalcification techniques for rat femurs using HE and immunohistochemical staining","volume":"2017","author":"Liu","year":"2017","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.acthis.2026.152375_bib11","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1038\/nri2094","article-title":"Cytokines in the pathogenesis of rheumatoid arthritis","volume":"7","author":"McInnes","year":"2007","journal-title":"Nat. Rev. Immunol."},{"issue":"8","key":"10.1016\/j.acthis.2026.152375_bib12","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1038\/s41379-020-0503-6","article-title":"Effect of decalcification protocols on immunohistochemistry and molecular analyses of bone samples","volume":"33","author":"Miquelestorena-Standley","year":"2020","journal-title":"Mod. Pathol."},{"key":"10.1016\/j.acthis.2026.152375_bib13","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/s12891-016-0944-8","article-title":"Aggrecan heterogeneity in articular cartilage from patients with osteoarthritis","volume":"17","author":"Mort","year":"2016","journal-title":"BMC Musculoskelet. Disord."},{"issue":"2","key":"10.1016\/j.acthis.2026.152375_bib14","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.4081\/ejh.2014.2371","article-title":"Histochemistry as a unique approach for investigating normal and osteoarthritic cartilage","volume":"58","author":"Musumeci","year":"2014","journal-title":"Eur. J. Histochem."},{"issue":"12","key":"10.1016\/j.acthis.2026.152375_bib15","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1369\/0022155417733708","article-title":"Comparison of different decalcification methods using rat mandibles as a model","volume":"65","author":"Savi","year":"2017","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.acthis.2026.152375_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.joca.2010.05.026","article-title":"Basic methods in histopathology of joint tissues","volume":"18","author":"Schmitz","year":"2010","journal-title":"Osteoarthr. Cartil."},{"issue":"4","key":"10.1016\/j.acthis.2026.152375_bib17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0196682","article-title":"Muscle wasting in osteoarthritis model induced by anterior cruciate ligament transection","volume":"13","author":"Silva","year":"2018","journal-title":"PLoS. One"},{"key":"10.1016\/j.acthis.2026.152375_bib18","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1007\/s10354-012-0168-y","article-title":"Osteoarthritis: histology and pathogenesis","volume":"163","author":"Sulzbacher","year":"2013","journal-title":"Wien. Med. Wochenschr."}],"container-title":["Acta Histochemica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0065128126000607?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0065128126000607?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T21:56:34Z","timestamp":1791064594000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0065128126000607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["S0065128126000607"],"URL":"https:\/\/doi.org\/10.1016\/j.acthis.2026.152375","relation":{},"ISSN":["0065-1281"],"issn-type":[{"value":"0065-1281","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effects of decalcification with 10% nitric acid solution and EDTA on Safranin O staining and aggrecan immunohistochemistry","name":"articletitle","label":"Article Title"},{"value":"Acta Histochemica","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.acthis.2026.152375","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier GmbH. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"152375"}}