{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:58:51Z","timestamp":1790355531834,"version":"4.1.0"},"reference-count":39,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["ajo.com","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["American Journal of Ophthalmology"],"published-print":{"date-parts":[[2007,6]]},"DOI":"10.1016\/j.ajo.2007.03.005","type":"journal-article","created":{"date-parts":[[2007,4,25]],"date-time":"2007-04-25T07:10:09Z","timestamp":1177485009000},"page":"945-953","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":77,"title":["Factors Influencing Outcomes in Cultivated Limbal Epithelial Transplantation for Chronic Cicatricial Ocular Surface Disorders"],"prefix":"10.1016","volume":"143","author":[{"given":"Jun","family":"Shimazaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunari","family":"Higa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumito","family":"Morito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murat","family":"Dogru","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Kawakita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Satake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeto","family":"Shimmura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Tsubota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ajo.2007.03.005_bib1","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/S0140-6736(96)11188-0","article-title":"Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium","volume":"349","author":"Pellegrini","year":"1997","journal-title":"Lancet"},{"key":"10.1016\/j.ajo.2007.03.005_bib2","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1056\/NEJM200007133430202","article-title":"Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells","volume":"343","author":"Tsai","year":"2000","journal-title":"N Engl J Med"},{"key":"10.1016\/j.ajo.2007.03.005_bib3","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1097\/00003226-200007000-00003","article-title":"Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease","volume":"19","author":"Schwab","year":"2000","journal-title":"Cornea"},{"key":"10.1016\/j.ajo.2007.03.005_bib4","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1097\/00007890-200111150-00002","article-title":"Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency","volume":"72","author":"Rama","year":"2001","journal-title":"Transplantation"},{"key":"10.1016\/j.ajo.2007.03.005_bib5","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1016\/S0161-6420(01)00694-7","article-title":"Cultivated corneal epithelial stem cell transplantation in ocular surface disorders","volume":"108","author":"Koizumi","year":"2001","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2007.03.005_bib6","first-page":"298","article-title":"Cultivated corneal epithelial transplantation for ocular surface reconstruction in acute phase of Stevens-Johnson syndrome","volume":"119","author":"Koizumi","year":"2001","journal-title":"Arch Ophthalmol"},{"key":"10.1016\/j.ajo.2007.03.005_bib7","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1016\/S0161-6420(02)01089-8","article-title":"Transplantation of human limbal epithelium cultivated on amniotic membrane for the treatment of severe ocular surface disorders","volume":"109","author":"Shimazaki","year":"2002","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2007.03.005_bib8","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1136\/bjo.86.4.463","article-title":"Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures","volume":"86","author":"Meller","year":"2002","journal-title":"Br J Ophthalmol"},{"key":"10.1016\/j.ajo.2007.03.005_bib9","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1016\/S0161-6420(02)01105-3","article-title":"Phenotypic study of a case with successful transplantation of ex vivo expanded human limbal epithelium for unilateral total limbal stem cell deficiency","volume":"109","author":"Grueterich","year":"2002","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2007.03.005_bib10","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1097\/00003226-200307000-00016","article-title":"Use of autologous cultured limbal and conjunctival epithelium in a patient with severe bilateral ocular surface disease induced by acid injury: a case report of unique application","volume":"22","author":"Sangwan","year":"2003","journal-title":"Cornea"},{"key":"10.1016\/j.ajo.2007.03.005_bib11","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1097\/01.TP.0000110320.45678.30","article-title":"Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface","volume":"77","author":"Nishida","year":"2004","journal-title":"Transplantation"},{"key":"10.1016\/j.ajo.2007.03.005_bib12","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1097\/01.ico.0000151508.49565.8a","article-title":"Cultivated corneal epithelial transplantation for severe ocular surface disease in vernal keratoconjunctivitis","volume":"24","author":"Sangwan","year":"2005","journal-title":"Cornea"},{"key":"10.1016\/j.ajo.2007.03.005_bib13","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1016\/S0161-6420(02)01081-3","article-title":"Long-term outcomes of keratolimbal allograft for the treatment of severe ocular surface disorders","volume":"109","author":"Ilari","year":"2002","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2007.03.005_bib14","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1016\/S0161-6420(00)00214-1","article-title":"Association of preoperative tear function with surgical outcome in severe Stevens-Johnson syndrome","volume":"107","author":"Shimazaki","year":"2000","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2007.03.005_bib15","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/S0161-6420(02)00994-6","article-title":"Limbal stem cell transplantation in chronic inflammatory eye disease","volume":"109","author":"Samson","year":"2002","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2007.03.005_bib16","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.1016\/S0161-6420(99)90412-8","article-title":"Treatment of persistent corneal epithelial defect by autologous serum application","volume":"106","author":"Tsubota","year":"1999","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2007.03.005_bib17","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.ophtha.2004.09.023","article-title":"Outcomes and DNA analysis of ex vivo expanded stem cell allograft for ocular surface reconstruction","volume":"112","author":"Daya","year":"2005","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2007.03.005_bib18","first-page":"635","article-title":"Functional reconstruction of rabbit corneal epithelium by human limbal cells cultured on amniotic membrane","volume":"8","author":"Du","year":"2003","journal-title":"Mol Vis"},{"key":"10.1016\/j.ajo.2007.03.005_bib19","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1136\/bjo.87.12.1509","article-title":"Corneal stromal changes following reconstruction by ex vivo expanded limbal epithelial cells in rabbits with total limbal stem cell deficiency","volume":"87","author":"Espana","year":"2003","journal-title":"Br J Ophthalmol"},{"key":"10.1016\/j.ajo.2007.03.005_bib20","doi-asserted-by":"crossref","first-page":"4230","DOI":"10.1167\/iovs.02-0943","article-title":"Modulation of keratin and connexin expression in limbal epithelium expanded on denuded amniotic membrane with and without a 3T3 fibroblast feeder layer","volume":"44","author":"Grueterich","year":"2003","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.ajo.2007.03.005_bib21","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.exer.2004.02.015","article-title":"Phenotypic characterization of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures","volume":"79","author":"Kim","year":"2004","journal-title":"Exp Eye Res"},{"key":"10.1016\/j.ajo.2007.03.005_bib22","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1097\/00003226-200001000-00013","article-title":"Amniotic membrane as a substrate for cultivating limbal corneal epithelial cells for autologous transplantation in rabbits","volume":"19","author":"Koizumi","year":"2000","journal-title":"Cornea"},{"key":"10.1016\/j.ajo.2007.03.005_bib23","first-page":"2114","article-title":"An evaluation of cultivated corneal limbal epithelial cells, using cell-suspension culture","volume":"43","author":"Koizumi","year":"2002","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.ajo.2007.03.005_bib24","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1097\/00003226-200207000-00013","article-title":"A fibrin-based bioengineered ocular surface with human corneal epithelial stem cells","volume":"21","author":"Han","year":"2002","journal-title":"Cornea"},{"key":"10.1016\/j.ajo.2007.03.005_bib25","first-page":"2584","article-title":"Factors affecting outcome following transplantation of ex vivo expanded limbal epithelium on amniotic membrane for total limbal deficiency in rabbits","volume":"43","author":"Ti","year":"2002","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.ajo.2007.03.005_bib26","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1136\/bjo.2003.026054","article-title":"Correlation of long- term phenotypic and clinical outcomes following limbal epithelial transplantation cultivated on amniotic membrane in rabbits","volume":"88","author":"Ti","year":"2004","journal-title":"Br J Ophthalmol"},{"key":"10.1016\/j.ajo.2007.03.005_bib27","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1056\/NEJM199906033402201","article-title":"Treatment of severe ocular-surface disorders with corneal epithelial stem-cell transplantation","volume":"340","author":"Tsubota","year":"1999","journal-title":"N Engl J Med"},{"key":"10.1016\/j.ajo.2007.03.005_bib28","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/S0161-6420(02)00960-0","article-title":"Long-term outcome of keratolimbal allograft with or without penetrating keratoplasty for total limbal stem cell deficiency","volume":"109","author":"Solomon","year":"2002","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2007.03.005_bib29","first-page":"63","article-title":"Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane","volume":"43","author":"Grueterich","year":"2002","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.ajo.2007.03.005_bib30","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1001\/archopht.120.6.783","article-title":"Human limbal progenitor cells expanded on intact amniotic membrane ex vivo","volume":"120","author":"Grueterich","year":"2002","journal-title":"Arch Ophthalmol"},{"key":"10.1016\/j.ajo.2007.03.005_bib31","first-page":"2506","article-title":"Cultivation of corneal epithelial cells on intact and denuded human amniotic membrane","volume":"41","author":"Koizumi","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.ajo.2007.03.005_bib32","first-page":"891","article-title":"Cultured corneal epithelia for ocular surface disease","volume":"97","author":"Schwab","year":"1999","journal-title":"Trans Am Ophthalmol Soc"},{"key":"10.1016\/j.ajo.2007.03.005_bib33","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0092-8674(75)80001-8","article-title":"Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells","volume":"6","author":"Rheinwald","year":"1975","journal-title":"Cell"},{"key":"10.1016\/j.ajo.2007.03.005_bib34","first-page":"2672","article-title":"In vitro propagation of human ocular surface epithelial cells for transplantation","volume":"34","author":"Lindberg","year":"1993","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.ajo.2007.03.005_bib35","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1038\/269489a0","article-title":"Cultured epithelial cells of cornea, conjunctiva and skin: absence of marked intrinsic divergence of their differentiated states","volume":"269","author":"Sun","year":"1977","journal-title":"Nature"},{"key":"10.1016\/j.ajo.2007.03.005_bib36","doi-asserted-by":"crossref","first-page":"4698","DOI":"10.1167\/iovs.03-0272","article-title":"Propagation and phenotypic preservation of rabbit limbal epithelial cells on amniotic membrane","volume":"44","author":"Wang","year":"2003","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.ajo.2007.03.005_bib37","first-page":"1814","article-title":"In vitro growth and differentiation of rabbit bulbar, fornix, and palpebral conjunctival epithelia","volume":"34","author":"Wei","year":"1993","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.ajo.2007.03.005_bib38","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/S0014-4835(03)00033-2","article-title":"Comparison of ultra-structure, tight junction-related protein expression and barrier function of human corneal epithelial cells cultivated on amniotic membrane with and without air-lifting","volume":"76","author":"Ban","year":"2003","journal-title":"Exp Eye Res"},{"key":"10.1016\/j.ajo.2007.03.005_bib39","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1167\/iovs.03-1361","article-title":"Development of a conjunctival epithelial equivalent with improved proliferative properties using a multi-step serum-free culture system","volume":"45","author":"Ang","year":"2004","journal-title":"Invest Ophthalmol Vis Sci"}],"container-title":["American Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002939407002449?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002939407002449?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T12:54:01Z","timestamp":1757595241000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0002939407002449"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["S0002939407002449"],"URL":"https:\/\/doi.org\/10.1016\/j.ajo.2007.03.005","relation":{},"ISSN":["0002-9394"],"issn-type":[{"value":"0002-9394","type":"print"}],"subject":[],"published":{"date-parts":[[2007,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Factors Influencing Outcomes in Cultivated Limbal Epithelial Transplantation for Chronic Cicatricial Ocular Surface Disorders","name":"articletitle","label":"Article Title"},{"value":"American Journal of Ophthalmology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ajo.2007.03.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2007 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}