{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:30:48Z","timestamp":1786984248607,"version":"build-2736575974"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["ajo.com","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["American Journal of Ophthalmology"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1016\/j.ajo.2020.07.004","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T22:21:08Z","timestamp":1594419668000},"page":"121-131","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"special_numbering":"C","title":["Three-Year Outcomes of Tri-Folded Endothelium-In Descemet Membrane Endothelial Keratoplasty With Pull-Through Technique"],"prefix":"10.1016","volume":"219","author":[{"given":"Angeli Christy","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Myerscough","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossella","family":"Spena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiorella","family":"Fusco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2844-774X","authenticated-orcid":false,"given":"Sergiu","family":"Socea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Furiosi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"De Rosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Bovone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3635-3521","authenticated-orcid":false,"given":"Massimo","family":"Busin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.ajo.2020.07.004_bib1","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.transproceed.2014.10.040","article-title":"Corneal transplantation activity over 7 years: changing trends for indications, patient demographics and surgical techniques from the Corneal Transplant Epidemiological Study (CORTES)","volume":"47","author":"Frigo","year":"2015","journal-title":"Transplant Proc"},{"issue":"2","key":"10.1016\/j.ajo.2020.07.004_bib3","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.ophtha.2017.08.015","article-title":"Descemet membrane endothelial keratoplasty: safety and outcomes","volume":"125","author":"Deng","year":"2018","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2020.07.004_bib4","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.2147\/OPTH.S212871","article-title":"Perceived difficulties and barriers to uptake of Descemet's membrane endothelial keratoplasty among surgeons","volume":"13","author":"Zafar","year":"2019","journal-title":"Clin Ophthalmol"},{"issue":"3","key":"10.1016\/j.ajo.2020.07.004_bib5","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.ophtha.2015.10.050","article-title":"Contact lens-assisted pull-through techinique for delivery of tri-folded (endothelium in) DMEK grafts minimizes surgical time and cell loss","volume":"123","author":"Busin","year":"2016","journal-title":"Ophthalmology"},{"issue":"5","key":"10.1016\/j.ajo.2020.07.004_bib6","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/j.ajo.2013.05.041","article-title":"Novel technique for the preparation of corneal grafts for Descemet membrane endothelial keratoplasty","volume":"156","author":"Muraine","year":"2013","journal-title":"Am J Ophthalmol"},{"issue":"9","key":"10.1016\/j.ajo.2020.07.004_bib7","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1097\/ICO.0000000000001650","article-title":"Endothelium-in versus endothelium-out insertion with Descemet membrane endothelial keratoplasty","volume":"37","author":"Price","year":"2018","journal-title":"Cornea"},{"issue":"3","key":"10.1016\/j.ajo.2020.07.004_bib8","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1097\/ICO.0000000000002194","article-title":"Quantitative and qualitative differences in endothelial cell loss between endothelium-in versus endothelium-out loading in Descemet membrane endothelial keratoplasty","volume":"39","author":"Ho","year":"2020","journal-title":"Cornea"},{"issue":"1","key":"10.1016\/j.ajo.2020.07.004_bib9","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1097\/ICO.0000000000002014","article-title":"Evaluation of total donor endothelial viability after endothelium-inward versus endothelium-outward loading and insertion in Descemet membrane endothelial keratoplasty","volume":"39","author":"Chong","year":"2020","journal-title":"Cornea"},{"issue":"5","key":"10.1016\/j.ajo.2020.07.004_bib10","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1097\/ICO.0b013e31825de8fa","article-title":"Comparison of manual and automated endothelial cell density analysis in normal eyes and DSEK eyes","volume":"32","author":"Price","year":"2013","journal-title":"Cornea"},{"key":"10.1016\/j.ajo.2020.07.004_bib11","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.ajo.2016.08.038","article-title":"Midterm results of Descemet membrane endothelial keratoplasty: 4 to 7 years clinical outcome","volume":"171","author":"Ham","year":"2016","journal-title":"Am J Ophthalmol"},{"issue":"10","key":"10.1016\/j.ajo.2020.07.004_bib12","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1016\/j.ophtha.2018.03.050","article-title":"Five-year graft survival of Descemet membrane endothelial keratoplasty (EK) versus Descemet stripping EK and the effect of donor sex matching","volume":"125","author":"Price","year":"2018","journal-title":"Ophthalmology"},{"issue":"6","key":"10.1016\/j.ajo.2020.07.004_bib13","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1097\/ICO.0000000000000417","article-title":"Cornea preservation time study: methods and potential impact on the cornea donor pool in the United States","volume":"34","author":"Lass","year":"2015","journal-title":"Cornea"},{"issue":"5","key":"10.1016\/j.ajo.2020.07.004_bib14","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1097\/ICO.0000000000002268","article-title":"Descemet membrane endothelial keratoplasty with a pull-through insertion device: surgical technique, endothelial cell loss, and early clinical results","volume":"39","author":"Tan","year":"2020","journal-title":"Cornea"},{"issue":"5","key":"10.1016\/j.ajo.2020.07.004_bib15","doi-asserted-by":"crossref","first-page":"e155","DOI":"10.1016\/j.jcjo.2017.03.006","article-title":"Pull-through technique for delivery of a larger diameter DMEK graft using endothelium-in method","volume":"52","author":"Romano","year":"2017","journal-title":"Can J Ophthalmol"},{"issue":"11","key":"10.1016\/j.ajo.2020.07.004_bib16","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1001\/jamaophthalmol.2015.3064","article-title":"Endothelial survival after Descemet membrane endothelial keratoplasty effect of surgical indication and graft adherence status","volume":"133","author":"Baydoun","year":"2015","journal-title":"JAMA Ophthalmol"},{"issue":"6","key":"10.1016\/j.ajo.2020.07.004_bib17","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1097\/ICO.0000000000001176","article-title":"Two-year clinical outcome of 500 consecutive cases undergoing Descemet membrane endothelial keratoplasty","volume":"36","author":"Peraza-Nieves","year":"2017","journal-title":"Cornea"},{"issue":"12","key":"10.1016\/j.ajo.2020.07.004_bib18","doi-asserted-by":"crossref","first-page":"8409","DOI":"10.1167\/iovs.14-15683","article-title":"A mathematical model to predict endothelial cell density following penetrating keratoplasty with selective dropout from graft failure","volume":"55","author":"Riddlesworth","year":"2014","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"10","key":"10.1016\/j.ajo.2020.07.004_bib19","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1097\/ICO.0b013e3181d21d07","article-title":"Long-term graft survival in penetrating keratoplasty: the biexponential model of chronic endothelial cell loss revisited","volume":"29","author":"B\u00f6hringer","year":"2010","journal-title":"Cornea"},{"issue":"3","key":"10.1016\/j.ajo.2020.07.004_bib20","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1097\/ICO.0000000000002120","article-title":"Five-year graft survival and clinical outcomes of 500 consecutive cases after Descemet membrane endothelial keratoplasty","volume":"39","author":"Birbal","year":"2020","journal-title":"Cornea"},{"key":"10.1016\/j.ajo.2020.07.004_bib21","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ajo.2019.10.017","article-title":"Parameters associated with endothelial cell density variability after Descemet membrane endothelial keratoplasty","volume":"211","author":"Oellerich","year":"2020","journal-title":"Am J Ophthalmol"},{"key":"10.1016\/j.ajo.2020.07.004_bib22","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.ajo.2018.06.013","article-title":"Clinical outcomes of preloaded Descemet membrane endothelial keratoplasty grafts with endothelium tri-folded inwards","volume":"193","author":"Busin","year":"2018","journal-title":"Am J Ophthalmol"},{"issue":"4","key":"10.1016\/j.ajo.2020.07.004_bib23","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.ophtha.2010.08.012","article-title":"Descemet's stripping endothelial keratoplasty five-year graft survival and endothelial cell loss","volume":"118","author":"Price","year":"2011","journal-title":"Ophthalmology"},{"issue":"1","key":"10.1016\/j.ajo.2020.07.004_bib24","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1055\/s-2008-1027127","article-title":"Prognosis in repeat keratoplasty: per indication analysis in a large monocentric cohort","volume":"225","author":"B\u00f6hringer","year":"2008","journal-title":"Klin Monbl Augenheilkd"},{"issue":"6","key":"10.1016\/j.ajo.2020.07.004_bib25","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1001\/jamaophthalmol.2014.352","article-title":"Descemet stripping automated endothelial keratoplasty after failed penetrating keratoplasty survival, rejection risk, and visual outcome","volume":"132","author":"Mitry","year":"2014","journal-title":"JAMA Ophthalmol"},{"issue":"5","key":"10.1016\/j.ajo.2020.07.004_bib26","doi-asserted-by":"crossref","first-page":"2279","DOI":"10.1167\/iovs.10-6187","article-title":"Corneal endothelial toxicity of air and SF6","volume":"52","author":"Landry","year":"2011","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"3","key":"10.1016\/j.ajo.2020.07.004_bib27","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1097\/ICO.0000000000001485","article-title":"Comparison of sulfur hexafluoride (SF6) and air tamponade in noniridectomized Descemet membrane endothelial keratoplasty","volume":"37","author":"Einan-Lifshitz","year":"2018","journal-title":"Cornea"},{"issue":"9","key":"10.1016\/j.ajo.2020.07.004_bib28","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1016\/j.ophtha.2015.05.013","article-title":"Comparison of sulfur hexafluoride 20% versus air tamponade in Descemet membrane endothelial keratoplasty","volume":"122","author":"G\u00fcell","year":"2015","journal-title":"Ophthalmology"},{"issue":"7","key":"10.1016\/j.ajo.2020.07.004_bib29","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1136\/bjophthalmol-2016-309653","article-title":"One-year outcome after Descemet membrane endothelial keratoplasty (DMEK) comparing sulfur hexafluoride (SF6) 20% versus 100% air for anterior chamber tamponade","volume":"101","author":"Schaub","year":"2017","journal-title":"Br J Ophthalmol"},{"issue":"9","key":"10.1016\/j.ajo.2020.07.004_bib30","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1097\/ICO.0000000000001999","article-title":"Five-year outcomes of ultrathin Descemet stripping automated endothelial keratoplasty","volume":"38","author":"Madi","year":"2019","journal-title":"Cornea"},{"issue":"2","key":"10.1016\/j.ajo.2020.07.004_bib31","first-page":"245","article-title":"Descemet stripping automated endothelial keratoplasty three-year graft and endothelial cell survival compared with penetrating keratoplasty","volume":"120","author":"Price","year":"2013","journal-title":"Ophthalmology"},{"issue":"4","key":"10.1016\/j.ajo.2020.07.004_bib32","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.ophtha.2016.11.001","article-title":"Cystoid macular edema after Descemet's stripping automated endothelial keratoplasty","volume":"124","author":"Kitazawa","year":"2017","journal-title":"Ophthalmology"},{"issue":"5","key":"10.1016\/j.ajo.2020.07.004_bib33","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/j.ajo.2011.10.004","article-title":"Graft rejection following Descemet stripping automated endothelial keratoplasty: features, risk factors, and outcomes","volume":"153","author":"Wu","year":"2012","journal-title":"Am J Ophthalmol"},{"issue":"1","key":"10.1016\/j.ajo.2020.07.004_bib34","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1136\/bjophthalmol-2014-305124","article-title":"Cystoid macular oedema following Descemet membrane endothelial keratoplasty","volume":"99","author":"Heinzelmann","year":"2015","journal-title":"Br J Ophthalmol"},{"issue":"3","key":"10.1016\/j.ajo.2020.07.004_bib35","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S0161-6420(00)00544-3","article-title":"The profile of repeated corneal transplantation","volume":"108","author":"Bersudsky","year":"2001","journal-title":"Ophthalmology"},{"issue":"1","key":"10.1016\/j.ajo.2020.07.004_bib36","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ophtha.2018.05.019","article-title":"Descemet endothelial thickness comparison trial: a randomized trial comparing ultrathin Descemet stripping automated endothelial keratoplasty with Descemet membrane endothelial keratoplasty","volume":"126","author":"Chamberlain","year":"2019","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2020.07.004_bib37","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1016\/j.ophtha.2020.02.029","article-title":"Descemet membrane endothelial keratoplasty versus ultrathin Descemet stripping automated endothelial keratoplasty: a multicenter randomized controlled clinical trial","volume":"127","author":"Dunker","year":"2020","journal-title":"Ophthalmology"}],"container-title":["American Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000293942030341X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000293942030341X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T13:42:49Z","timestamp":1757598169000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S000293942030341X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":36,"alternative-id":["S000293942030341X"],"URL":"https:\/\/doi.org\/10.1016\/j.ajo.2020.07.004","relation":{},"ISSN":["0002-9394"],"issn-type":[{"value":"0002-9394","type":"print"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Three-Year Outcomes of Tri-Folded Endothelium-In Descemet Membrane Endothelial Keratoplasty With Pull-Through Technique","name":"articletitle","label":"Article Title"},{"value":"American Journal of Ophthalmology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ajo.2020.07.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}