{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T08:10:38Z","timestamp":1793779838591,"version":"4.6.1"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100008001","name":"Universit\u00e4t zu K\u00f6ln","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000921","name":"European Cooperation in Science and Technology","doi-asserted-by":"publisher","award":["BM1302"],"award-info":[{"award-number":["BM1302"]}],"id":[{"id":"10.13039\/501100000921","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["FOR2240"],"award-info":[{"award-number":["FOR2240"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ajo.com","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["American Journal of Ophthalmology"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1016\/j.ajo.2020.09.030","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T02:02:19Z","timestamp":1600653739000},"page":"292-301","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"special_numbering":"C","title":["Fibrillar Layer as a Marker for Areas of Pronounced Corneal Endothelial Cell Loss in Advanced Fuchs Endothelial Corneal Dystrophy"],"prefix":"10.1016","volume":"222","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5176-5738","authenticated-orcid":false,"given":"Agathe","family":"Hribek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Clahsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Horstmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Siebelmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas","family":"Loreck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludwig M.","family":"Heindl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn O.","family":"Bachmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus","family":"Cursiefen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Matthaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ajo.2020.09.030_bib1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1146\/annurev-vision-091718-014852","article-title":"Fuchs endothelial corneal dystrophy: clinical, genetic, pathophysiologic, and therapeutic aspects","volume":"5","author":"Matthaei","year":"2019","journal-title":"Annu Rev Vis Sci"},{"issue":"2","key":"10.1016\/j.ajo.2020.09.030_bib2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1001\/jamaophthalmol.2015.4776","article-title":"Global survey of corneal transplantation and eye banking","volume":"134","author":"Gain","year":"2016","journal-title":"JAMA Ophthalmol"},{"key":"10.1016\/j.ajo.2020.09.030_bib3","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.ajo.2018.03.036","article-title":"Evolution of consecutive Descemet membrane endothelial keratoplasty outcomes throughout a 5-year period performed by two experienced surgeons","volume":"190","author":"Schrittenlocher","year":"2018","journal-title":"Am J Ophthalmol"},{"issue":"4","key":"10.1016\/j.ajo.2020.09.030_bib4","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.ophtha.2016.12.017","article-title":"Incidence and clinical course of immune reactions after Descemet membrane endothelial keratoplasty: retrospective analysis of 1000 consecutive eyes","volume":"124","author":"Hos","year":"2017","journal-title":"Ophthalmology"},{"key":"10.1016\/j.ajo.2020.09.030_bib5","doi-asserted-by":"crossref","first-page":"100768","DOI":"10.1016\/j.preteyeres.2019.07.001","article-title":"Immune reactions after modern lamellar (DALK, DSAEK, DMEK) versus conventional penetrating corneal transplantation","volume":"73","author":"Hos","year":"2019","journal-title":"Prog Retin Eye Res"},{"issue":"12","key":"10.1016\/j.ajo.2020.09.030_bib6","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1097\/ICO.0000000000000270","article-title":"Long-term follow-up and complications of stripping descemet membrane without placement of graft in eyes with Fuchs endothelial dystrophy","volume":"33","author":"Arbelaez","year":"2014","journal-title":"Cornea"},{"issue":"10","key":"10.1016\/j.ajo.2020.09.030_bib7","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1097\/ICO.0000000000000915","article-title":"Treatment of Fuchs endothelial dystrophy by Descemet stripping without endothelial keratoplasty","volume":"35","author":"Borkar","year":"2016","journal-title":"Cornea"},{"issue":"1","key":"10.1016\/j.ajo.2020.09.030_bib8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.ophtha.2012.08.037","article-title":"Spontaneous corneal clearing after Descemet's stripping","volume":"120","author":"Bleyen","year":"2013","journal-title":"Ophthalmology"},{"issue":"2","key":"10.1016\/j.ajo.2020.09.030_bib9","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1097\/ICO.0b013e3181eeb2c7","article-title":"Persistent corneal edema after descemetorhexis without corneal graft implantation in a case of fuchs endothelial dystrophy","volume":"30","author":"Ham","year":"2011","journal-title":"Cornea"},{"issue":"4","key":"10.1016\/j.ajo.2020.09.030_bib10","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1097\/ICU.0000000000000579","article-title":"Descemet's stripping without endothelial keratoplasty","volume":"30","author":"Garcerant","year":"2019","journal-title":"Curr Opin Ophthalmol"},{"issue":"1","key":"10.1016\/j.ajo.2020.09.030_bib11","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1097\/00003226-198801000-00002","article-title":"Fuchs' dystrophy","volume":"7","author":"Wilson","year":"1988","journal-title":"Cornea"},{"issue":"12","key":"10.1016\/j.ajo.2020.09.030_bib12","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1001\/archopht.1982.01030040932013","article-title":"The ultrastructure of Descemets membrane .3. Fuchs dystrophy","volume":"100","author":"Bourne","year":"1982","journal-title":"Arch Ophthalmol"},{"issue":"1","key":"10.1016\/j.ajo.2020.09.030_bib13","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1001\/archopht.1982.01030030124015","article-title":"Posterior collagenous layer of the cornea. Ultrastructural classification of abnormal collagenous tissue posterior to Descemet's membrane in 30 cases","volume":"100","author":"Waring","year":"1982","journal-title":"Arch Ophthalmol"},{"issue":"1","key":"10.1016\/j.ajo.2020.09.030_bib14","first-page":"9","article-title":"Electron microscopic studies on Fuchs combined dystrophy .1. Posterior portion of cornea","volume":"10","author":"Iwamoto","year":"1971","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"6","key":"10.1016\/j.ajo.2020.09.030_bib15","doi-asserted-by":"crossref","first-page":"3700","DOI":"10.1167\/iovs.14-14154","article-title":"Extracellular matrix alterations in late-onset Fuchs' corneal dystrophy","volume":"55","author":"Weller","year":"2014","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"1","key":"10.1016\/j.ajo.2020.09.030_bib16","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1167\/iovs.13-12689","article-title":"Endothelial cell microRNA expression in human late-onset Fuchs' dystrophy","volume":"55","author":"Matthaei","year":"2014","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"11","key":"10.1016\/j.ajo.2020.09.030_bib17","doi-asserted-by":"crossref","first-page":"2036","DOI":"10.1001\/archopht.1978.03910060424004","article-title":"Corneal endothelial dystrophy. A study of 64 families","volume":"96","author":"Krachmer","year":"1978","journal-title":"Arch Ophthalmol"},{"issue":"1","key":"10.1016\/j.ajo.2020.09.030_bib18","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1097\/ICO.0b013e31821c9b8f","article-title":"A multicenter study to map genes for Fuchs endothelial corneal dystrophy: baseline characteristics and heritability","volume":"31","author":"Louttit","year":"2012","journal-title":"Cornea"},{"issue":"4","key":"10.1016\/j.ajo.2020.09.030_bib19","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1080\/08820538.2019.1632355","article-title":"Imaging the corneal endothelium in Fuchs corneal endothelial dystrophy","volume":"34","author":"Ong Tone","year":"2019","journal-title":"Semin Ophthalmol"},{"issue":"1","key":"10.1016\/j.ajo.2020.09.030_bib20","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1001\/archopht.1981.03930010082007","article-title":"Endothelial mosaic in Fuchs' dystrophy. A qualitative evaluation with the specular microscope","volume":"99","author":"Laing","year":"1981","journal-title":"Arch Ophthalmol"},{"issue":"9","key":"10.1016\/j.ajo.2020.09.030_bib21","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1007\/s00347-018-0743-8","article-title":"Technique of Descemet membrane endothelial keratoplasty (DMEK): Video article [in German]","volume":"115","author":"Matthaei","year":"2018","journal-title":"Ophthalmologe"},{"issue":"7","key":"10.1016\/j.ajo.2020.09.030_bib22","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat Methods"},{"key":"10.1016\/j.ajo.2020.09.030_bib23","doi-asserted-by":"crossref","first-page":"23096","DOI":"10.1038\/srep23096","article-title":"The ultrastructures and mechanical properties of the Descement\u2018s membrane in Fuchs endothelial corneal dystrophy","volume":"6","author":"Xia","year":"2016","journal-title":"Sci Rep"},{"issue":"2","key":"10.1016\/j.ajo.2020.09.030_bib24","first-page":"200","article-title":"Type I collagen and fibronectin synthesis by retrocorneal fibrous membrane","volume":"22","author":"Kay","year":"1982","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"12","key":"10.1016\/j.ajo.2020.09.030_bib25","doi-asserted-by":"crossref","first-page":"4504","DOI":"10.1167\/iovs.05-0497","article-title":"Fuchs corneal dystrophy: aberrant collagen distribution in an L450W mutant of the COL8A2 gene","volume":"46","author":"Gottsch","year":"2005","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"2","key":"10.1016\/j.ajo.2020.09.030_bib26","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0039-6257(93)90099-S","article-title":"Fuchs endothelial dystrophy of the cornea","volume":"38","author":"Adamis","year":"1993","journal-title":"Surv Ophthalmol"},{"issue":"6","key":"10.1016\/j.ajo.2020.09.030_bib27","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/S0161-6420(82)34746-6","article-title":"The corneal endothelium. Normal and pathologic structure and function","volume":"89","author":"Waring","year":"1982","journal-title":"Ophthalmology"},{"issue":"3","key":"10.1016\/j.ajo.2020.09.030_bib28","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1097\/01.ico.0000148288.53323.b2","article-title":"A morphologic study of Fuchs dystrophy and bullous keratopathy","volume":"24","author":"Yuen","year":"2005","journal-title":"Cornea"},{"issue":"6","key":"10.1016\/j.ajo.2020.09.030_bib29","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1016\/j.ajo.2010.11.032","article-title":"Myofibroblast metaplasia after descemet membrane endothelial keratoplasty","volume":"151","author":"Heindl","year":"2011","journal-title":"Am J Ophthalmol"},{"issue":"9","key":"10.1016\/j.ajo.2020.09.030_bib30","first-page":"2501","article-title":"Corneal endothelial cell apoptosis in patients with Fuchs\u2018 dystrophy","volume":"41","author":"Borderie","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"8","key":"10.1016\/j.ajo.2020.09.030_bib31","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1001\/jamaophthalmol.2018.2031","article-title":"Association of the gutta-induced microenvironment with corneal endothelial cell behavior and demise in Fuchs endothelial corneal dystrophy","volume":"136","author":"Kocaba","year":"2018","journal-title":"JAMA Ophthalmol"},{"issue":"22","key":"10.1016\/j.ajo.2020.09.030_bib32","doi-asserted-by":"crossref","first-page":"2896","DOI":"10.1002\/adhm.201600848","article-title":"In\u00a0vitro topographical model of Fuchs dystrophy for evaluation of corneal endothelial cell monolayer formation","volume":"5","author":"Rizwan","year":"2016","journal-title":"Adv Healthc Mater"},{"issue":"13","key":"10.1016\/j.ajo.2020.09.030_bib33","doi-asserted-by":"crossref","first-page":"5880","DOI":"10.1167\/iovs.17-22286","article-title":"Label-free in\u00a0vivo imaging of corneal lymphatic vessels using microscopic optical coherence tomography","volume":"58","author":"Horstmann","year":"2017","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"1","key":"10.1016\/j.ajo.2020.09.030_bib34","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.exer.2011.10.018","article-title":"Culture of human corneal endothelial cells isolated from corneas with Fuchs endothelial corneal dystrophy","volume":"94","author":"Zaniolo","year":"2012","journal-title":"Exp Eye Res"},{"issue":"11","key":"10.1016\/j.ajo.2020.09.030_bib35","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1056\/NEJMoa1712770","article-title":"Injection of cultured cells with a ROCK inhibitor for bullous keratopathy","volume":"378","author":"Kinoshita","year":"2018","journal-title":"N Engl J Med"},{"issue":"7","key":"10.1016\/j.ajo.2020.09.030_bib36","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1097\/ICO.0000000000001578","article-title":"Outcomes of hemi-Descemet membrane endothelial keratoplasty for Fuchs endothelial corneal dystrophy","volume":"37","author":"Birbal","year":"2018","journal-title":"Cornea"},{"issue":"10","key":"10.1016\/j.ajo.2020.09.030_bib37","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1136\/bjophthalmol-2017-311398","article-title":"Quarter-Descemet membrane endothelial keratoplasty (Quarter-DMEK) for Fuchs endothelial corneal dystrophy: 6\u00a0months clinical outcome","volume":"102","author":"Zygoura","year":"2018","journal-title":"Br J Ophthalmol"},{"issue":"6","key":"10.1016\/j.ajo.2020.09.030_bib38","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1016\/S0161-6420(00)00087-7","article-title":"Keratopathy in pseudoexfoliation syndrome as a cause of corneal endothelial decompensation: a clinicopathologic study","volume":"107","author":"Naumann","year":"2000","journal-title":"Ophthalmology"}],"container-title":["American Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002939420305286?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002939420305286?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:00:58Z","timestamp":1777384858000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0002939420305286"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":38,"alternative-id":["S0002939420305286"],"URL":"https:\/\/doi.org\/10.1016\/j.ajo.2020.09.030","relation":{},"ISSN":["0002-9394"],"issn-type":[{"value":"0002-9394","type":"print"}],"subject":[],"published":{"date-parts":[[2021,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Fibrillar Layer as a Marker for Areas of Pronounced Corneal Endothelial Cell Loss in Advanced Fuchs Endothelial Corneal Dystrophy","name":"articletitle","label":"Article Title"},{"value":"American Journal of Ophthalmology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ajo.2020.09.030","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}