{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T01:29:40Z","timestamp":1793496580455,"version":"4.5.0"},"reference-count":27,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T00:00:00Z","timestamp":1642636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["ajog.org","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["American Journal of Obstetrics and Gynecology"],"published-print":{"date-parts":[[2022,8]]},"DOI":"10.1016\/j.ajog.2022.01.019","type":"journal-article","created":{"date-parts":[[2022,1,24]],"date-time":"2022-01-24T20:44:54Z","timestamp":1643057094000},"page":"259.e1-259.e14","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":57,"title":["Cell-free DNA screening for trisomies 21, 18, and 13 in pregnancies at low and high risk for aneuploidy with genetic confirmation"],"prefix":"10.1016","volume":"227","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9254-4261","authenticated-orcid":false,"given":"Pe\u2019er","family":"Dar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5079-2374","authenticated-orcid":false,"given":"Bo","family":"Jacobsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cora","family":"MacPherson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0708-7498","authenticated-orcid":false,"given":"Melissa","family":"Egbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fergal","family":"Malone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald J.","family":"Wapner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley S.","family":"Roman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Khalil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Revital","family":"Faro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajeevi","family":"Madankumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lance","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sina","family":"Haeri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Silver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi","family":"Vohra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon","family":"Hyett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garfield","family":"Clunie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8796-2381","authenticated-orcid":false,"given":"Zachary","family":"Demko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Rabinowitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Flood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1414-7279","authenticated-orcid":false,"given":"Ylva","family":"Carlsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5574-235X","authenticated-orcid":false,"given":"Georgios","family":"Doulaveris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3800-394X","authenticated-orcid":false,"given":"Ciara","family":"Malone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Hallingstrom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Klugman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Clifton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlly","family":"Kao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hakon","family":"Hakonarson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E.","family":"Norton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ajog.2022.01.019_bib1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1002\/pd.4136","article-title":"Noninvasive prenatal testing: the paradigm is shifting rapidly","volume":"33","author":"Chitty","year":"2013","journal-title":"Prenat Diagn"},{"key":"10.1016\/j.ajog.2022.01.019_bib2","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1056\/NEJMra1705345","article-title":"Sequencing of circulating cell-free DNA during pregnancy","volume":"379","author":"Bianchi","year":"2018","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.ajog.2022.01.019_bib3","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1002\/uog.17484","article-title":"Analysis of cell-free DNA in maternal blood in screening for aneuploidies: updated meta-analysis","volume":"50","author":"Gil","year":"2017","journal-title":"Ultrasound Obstet Gynecol"},{"key":"10.1016\/j.ajog.2022.01.019_bib4","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1056\/NEJMoa1407349","article-title":"Cell-free DNA analysis for noninvasive examination of trisomy","volume":"372","author":"Norton","year":"2015","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.ajog.2022.01.019_bib5","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1002\/pd.4608","article-title":"Position statement from the Chromosome Abnormality Screening Committee on behalf of the Board of the International Society for Prenatal Diagnosis","volume":"35","author":"Benn","year":"2015","journal-title":"Prenat Diagn"},{"key":"10.1016\/j.ajog.2022.01.019_bib6","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1056\/NEJMoa1311037","article-title":"DNA sequencing versus standard prenatal aneuploidy screening","volume":"370","author":"Bianchi","year":"2014","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.ajog.2022.01.019_bib7","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1038\/gim.2016.97","article-title":"Noninvasive prenatal screening for fetal aneuploidy, 2016 update: a position statement of the American College of Medical Genetics and Genomics","volume":"18","author":"Gregg","year":"2016","journal-title":"Genet Med"},{"key":"10.1016\/j.ajog.2022.01.019_bib8","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1097\/AOG.0000000000004107","article-title":"Screening for fetal chromosomal abnormalities: ACOG practice bulletin summary, Number 226","volume":"136","year":"2020","journal-title":"Obstet Gynecol"},{"key":"10.1016\/j.ajog.2022.01.019_bib9","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1002\/pd.4103","article-title":"Validation of targeted sequencing of single-nucleotide polymorphisms for non-invasive prenatal detection of aneuploidy of chromosomes 13, 18, 21, X, and Y","volume":"33","author":"Nicolaides","year":"2013","journal-title":"Prenat Diagn"},{"key":"10.1016\/j.ajog.2022.01.019_bib10","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1159\/000442931","article-title":"Validation of an enhanced version of a single-nucleotide polymorphism-based noninvasive prenatal test for detection of fetal aneuploidies","volume":"40","author":"Ryan","year":"2016","journal-title":"Fetal Diagn Ther"},{"key":"10.1016\/j.ajog.2022.01.019_bib11","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1002\/uog.11083","article-title":"Non-invasive prenatal diagnosis for Down syndrome: the paradigm will shift, but slowly","volume":"39","author":"Benn","year":"2012","journal-title":"Ultrasound Obstet Gynecol"},{"key":"10.1016\/j.ajog.2022.01.019_bib12","doi-asserted-by":"crossref","first-page":"527.e1","DOI":"10.1016\/j.ajog.2014.08.006","article-title":"Clinical experience and follow-up with large scale single-nucleotide polymorphism-based noninvasive prenatal aneuploidy testing","volume":"211","author":"Dar","year":"2014","journal-title":"Am J Obstet Gynecol"},{"key":"10.1016\/j.ajog.2022.01.019_bib13","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1002\/uog.14792","article-title":"Non-invasive prenatal testing for trisomies 21, 18 and 13: clinical experience from 146,958 pregnancies","volume":"45","author":"Zhang","year":"2015","journal-title":"Ultrasound Obstet Gynecol"},{"key":"10.1016\/j.ajog.2022.01.019_bib14","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1056\/NEJMe1502441","article-title":"Use of cell-free DNA to screen for Down\u2019s syndrome","volume":"372","author":"Chitty","year":"2015","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.ajog.2022.01.019_bib15","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1097\/AOG.0000000000000363","article-title":"Single-nucleotide polymorphism-based noninvasive prenatal screening in a high-risk and low-risk cohort","volume":"124","author":"Pergament","year":"2014","journal-title":"Obstet Gynecol"},{"key":"10.1016\/j.ajog.2022.01.019_bib16","unstructured":"Google Inc., Mountain View, CA. Available at: https:\/\/www.usenix.org\/system\/files\/conference\/osdi16\/osdi16-abadi.pdf."},{"key":"10.1016\/j.ajog.2022.01.019_bib18","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1056\/NEJMoa043693","article-title":"First-trimester or second-trimester screening, or both, for Down\u2019s syndrome","volume":"353","author":"Malone","year":"2005","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.ajog.2022.01.019_bib19","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1086\/301800","article-title":"Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis","volume":"62","author":"Lo","year":"1998","journal-title":"Am J Hum Genet"},{"key":"10.1016\/j.ajog.2022.01.019_bib20","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1002\/pd.4119","article-title":"Gestational age and maternal weight effects on fetal cell-free DNA in maternal plasma","volume":"33","author":"Wang","year":"2013","journal-title":"Prenat Diagn"},{"key":"10.1016\/j.ajog.2022.01.019_bib21","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1002\/pd.4126","article-title":"The impact of maternal plasma DNA fetal fraction on next generation sequencing tests for common fetal aneuploidies","volume":"33","author":"Canick","year":"2013","journal-title":"Prenat Diagn"},{"key":"10.1016\/j.ajog.2022.01.019_bib22","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/uog.19176","article-title":"Fetal fraction-based risk algorithm for non-invasive prenatal testing: screening for trisomies 13 and 18 and triploidy in women with low cell-free fetal DNA","volume":"53","author":"McKanna","year":"2019","journal-title":"Ultrasound Obstet Gynecol"},{"key":"10.1016\/j.ajog.2022.01.019_bib23","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/uog.1923","article-title":"Placental volume measured by three-dimensional ultrasound at 11 to 13\u00a0+ 6 weeks of gestation: relation to chromosomal defects","volume":"26","author":"Wegrzyn","year":"2005","journal-title":"Ultrasound Obstet Gynecol"},{"key":"10.1016\/j.ajog.2022.01.019_bib24","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1002\/pd.4541","article-title":"Circulating cell free DNA testing: are some test failures informative?","volume":"35","author":"Palomaki","year":"2015","journal-title":"Prenat Diagn"},{"key":"10.1016\/j.ajog.2022.01.019_bib25","unstructured":"Organization for Economic Co-operation and Development. OECD family database. 2021. Available at: http:\/\/www.oecd.org\/els\/family\/database.htm. Accessed January 20, 2021."},{"key":"10.1016\/j.ajog.2022.01.019_bib26","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1046\/j.1469-0705.1999.13030167.x","article-title":"Maternal age- and gestation-specific risk for trisomy 21","volume":"13","author":"Snijders","year":"1999","journal-title":"Ultrasound Obstet Gynecol"},{"key":"10.1016\/j.ajog.2022.01.019_bib27","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1002\/(SICI)1097-0223(199902)19:2<142::AID-PD486>3.0.CO;2-7","article-title":"Fetal loss in Down syndrome pregnancies","volume":"19","author":"Morris","year":"1999","journal-title":"Prenat Diagn"},{"key":"10.1016\/j.ajog.2022.01.019_bib28","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1002\/ajmg.a.32220","article-title":"The risk of fetal loss following a prenatal diagnosis of trisomy 13 or trisomy 18","volume":"146A","author":"Morris","year":"2008","journal-title":"Am J Med Genet A"}],"container-title":["American Journal of Obstetrics and Gynecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002937822000412?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002937822000412?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T11:37:05Z","timestamp":1757590625000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0002937822000412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["S0002937822000412"],"URL":"https:\/\/doi.org\/10.1016\/j.ajog.2022.01.019","relation":{},"ISSN":["0002-9378"],"issn-type":[{"value":"0002-9378","type":"print"}],"subject":[],"published":{"date-parts":[[2022,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cell-free DNA screening for trisomies 21, 18, and 13 in pregnancies at low and high risk for aneuploidy with genetic confirmation","name":"articletitle","label":"Article Title"},{"value":"American Journal of Obstetrics and Gynecology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ajog.2022.01.019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}