{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:20:37Z","timestamp":1790252437622,"version":"4.1.0"},"reference-count":49,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,12,13]],"date-time":"2019-12-13T00:00:00Z","timestamp":1576195200000},"content-version":"vor","delay-in-days":1045,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"Department of Laboratory Medicine"}],"content-domain":{"domain":["clinicalkey.jp","journals.elsevierhealth.com","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The American Journal of Pathology"],"published-print":{"date-parts":[[2017,2]]},"DOI":"10.1016\/j.ajpath.2016.10.007","type":"journal-article","created":{"date-parts":[[2016,12,18]],"date-time":"2016-12-18T15:45:32Z","timestamp":1482075932000},"page":"304-317","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"title":["Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting"],"prefix":"10.1016","volume":"187","author":[{"given":"Jonathan R.","family":"Fromm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anju","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent L.","family":"Wood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ajpath.2016.10.007_bib1","series-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","first-page":"323","article-title":"Nodular lymphocyte predominant Hodgkin lymphoma","author":"Poppema","year":"2008"},{"key":"10.1016\/j.ajpath.2016.10.007_bib2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1146\/annurev.pathol.4.110807.092209","article-title":"Pathogenesis of classical and lymphocyte-predominant Hodgkin lymphoma","volume":"4","author":"Schmitz","year":"2009","journal-title":"Annu Rev Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib3","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1309\/38RK238FCDCH5R22","article-title":"Nodular lymphocyte predominant Hodgkin lymphoma. An immunophenotypic reappraisal based on a single-institution experience","volume":"119","author":"Uherova","year":"2003","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib4","series-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","first-page":"326","article-title":"Classical Hodgkin Lymphoma, introduction","author":"Stein","year":"2008"},{"key":"10.1016\/j.ajpath.2016.10.007_bib5","first-page":"220","article-title":"Hodgkin's lymphomas: classification, diagnosis, and grading","volume":"36","author":"Harris","year":"1999","journal-title":"Semin Hematol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib6","first-page":"159","article-title":"Hodgkin's disease: classification and differential diagnosis","volume":"12","author":"Harris","year":"1999","journal-title":"Mod Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib7","series-title":"Henry's Clinical Diagnosis and Management by Laboratory Methods","first-page":"656","article-title":"The flow cytometric evaluation of hematopoietic neoplasia","author":"Wood","year":"2011"},{"key":"10.1016\/j.ajpath.2016.10.007_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-61737-950-5_1","article-title":"Flow cytometry: an introduction","volume":"699","author":"Givan","year":"2011","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib9","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1309\/AJCP0Q1SVOXBHMAM","article-title":"A six-color flow cytometry assay for immunophenotyping classical Hodgkin lymphoma in lymph nodes","volume":"141","author":"Fromm","year":"2014","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib10","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1309\/AJCPW3UN9DYLDSPB","article-title":"Flow cytometry can diagnose classical hodgkin lymphoma in lymph nodes with high sensitivity and specificity","volume":"131","author":"Fromm","year":"2009","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib11","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/978-1-62703-269-8_2","article-title":"Flow cytometry for non-Hodgkin and classical Hodgkin lymphoma","volume":"971","author":"Wu","year":"2013","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib12","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1309\/7371XK6F6P7474XX","article-title":"Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting","volume":"126","author":"Fromm","year":"2006","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib13","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.ymeth.2012.03.028","article-title":"Strategies for immunophenotyping and purifying classical Hodgkin lymphoma cells from lymph nodes by flow cytometry and flow cytometric cell sorting","volume":"57","author":"Fromm","year":"2012","journal-title":"Methods"},{"key":"10.1016\/j.ajpath.2016.10.007_bib14","series-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","first-page":"322","article-title":"Hodgkin lymphoma-introduction","author":"Stein","year":"2008"},{"key":"10.1016\/j.ajpath.2016.10.007_bib15","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0140-6736(74)91602-X","article-title":"Letter: Origin of Hodgkin's cell","volume":"2","author":"Kadin","year":"1974","journal-title":"Lancet"},{"key":"10.1016\/j.ajpath.2016.10.007_bib16","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/S0140-6736(77)90281-1","article-title":"Reed-Sternberg-cell\/lymphocyte interaction","volume":"2","author":"Payne","year":"1977","journal-title":"Lancet"},{"key":"10.1016\/j.ajpath.2016.10.007_bib17","first-page":"7","article-title":"The Reed-Sternberg cell\/lymphocyte interaction: ultrastructure and characteristics of binding","volume":"100","author":"Payne","year":"1980","journal-title":"Am J Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib18","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1002\/path.1711220205","article-title":"Rosetting and other reactions of the Reed-Sternberg cell","volume":"122","author":"Stuart","year":"1977","journal-title":"J Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib19","first-page":"37","article-title":"Molecular pathways of adhesion in spontaneous rosetting of T-lymphocytes to the Hodgkin's cell line L428","volume":"48","author":"Sanders","year":"1988","journal-title":"Cancer Res"},{"key":"10.1016\/j.ajpath.2016.10.007_bib20","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1002\/1097-0142(19850215)55:4<683::AID-CNCR2820550402>3.0.CO;2-O","article-title":"Morphologic, immunologic, enzymehistochemical and chromosomal analysis of a cell line derived from Hodgkin's disease. Evidence for a B-cell origin of Sternberg-Reed cells","volume":"55","author":"Poppema","year":"1985","journal-title":"Cancer"},{"key":"10.1016\/j.ajpath.2016.10.007_bib21","first-page":"42","article-title":"Hodgkin's disease derived cell lines: a review","volume":"5","author":"Drexler","year":"1992","journal-title":"Hum Cell"},{"key":"10.1016\/j.ajpath.2016.10.007_bib22","doi-asserted-by":"crossref","first-page":"4918","DOI":"10.1182\/blood.V87.11.4918b.bloodjournal87114918b","article-title":"CD40: a sensitive marker of Reed-Sternberg cells","volume":"87","author":"Carbone","year":"1996","journal-title":"Blood"},{"key":"10.1016\/j.ajpath.2016.10.007_bib23","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1182\/blood.V85.3.780.bloodjournal853780","article-title":"Expression of functional CD40 antigen on Reed-Sternberg cells and Hodgkin's disease cell lines","volume":"85","author":"Carbone","year":"1995","journal-title":"Blood"},{"key":"10.1016\/j.ajpath.2016.10.007_bib24","first-page":"1281","article-title":"L and H cells of nodular lymphocyte predominant Hodgkin's disease show immunoglobulin light-chain restriction","volume":"139","author":"Schmid","year":"1991","journal-title":"Am J Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib25","first-page":"812","article-title":"Lymphocyte predominance Hodgkin's disease. Evidence for a kappa light chain-restricted monotypic B-cell neoplasm","volume":"146","author":"Stoler","year":"1995","journal-title":"Am J Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib26","doi-asserted-by":"crossref","first-page":"862","DOI":"10.5858\/2002-126-0862-BCMILP","article-title":"B-cell markers in lymphocyte predominance Hodgkin disease","volume":"126","author":"Torlakovic","year":"2002","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.ajpath.2016.10.007_bib27","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0950-3536(96)80019-9","article-title":"Pathophysiology of Hodgkin's disease: functional and molecular aspects","volume":"9","author":"Gruss","year":"1996","journal-title":"Baillieres Clin Haematol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib28","doi-asserted-by":"crossref","first-page":"3462","DOI":"10.1158\/1078-0432.CCR-13-0855","article-title":"PD-L1 expression is characteristic of a subset of aggressive B-cell lymphomas and virus-associated malignancies","volume":"19","author":"Chen","year":"2013","journal-title":"Clin Cancer Res"},{"key":"10.1016\/j.ajpath.2016.10.007_bib29","doi-asserted-by":"crossref","first-page":"2517","DOI":"10.1056\/NEJMe1205943","article-title":"Tumor immunotherapy directed at PD-1","volume":"366","author":"Ribas","year":"2012","journal-title":"N Engl J Med"},{"key":"10.1016\/j.ajpath.2016.10.007_bib30","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1309\/57G0-1BNF-KB9R-L4HN","article-title":"Lack of surface immunoglobulin light chain expression by flow cytometric immunophenotyping can help diagnose peripheral B-cell lymphoma","volume":"118","author":"Li","year":"2002","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib31","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1097\/01.pas.0000194741.87798.45","article-title":"IgD positive L&H cells identify a unique subset of nodular lymphocyte predominant Hodgkin lymphoma","volume":"30","author":"Prakash","year":"2006","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib32","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1080\/1042819031000149421","article-title":"Diffuse large B-cell lymphoma arising in nodular lymphocyte predominant Hodgkin lymphoma: a report of 21 cases from the Nebraska Lymphoma Study Group","volume":"45","author":"Huang","year":"2004","journal-title":"Leuk Lymphoma"},{"key":"10.1016\/j.ajpath.2016.10.007_bib33","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1097\/PAS.0b013e31822832de","article-title":"Nodular lymphocyte-predominant hodgkin lymphoma with atypical T cells: a morphologic variant mimicking peripheral T-cell lymphoma","volume":"35","author":"Sohani","year":"2011","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib34","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1038\/leu.2010.302","article-title":"The isotype of the BCR as a surrogate for the GCB and ABC molecular subtypes in diffuse large B-cell lymphoma","volume":"25","author":"Ruminy","year":"2011","journal-title":"Leukemia"},{"key":"10.1016\/j.ajpath.2016.10.007_bib35","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1038\/sj.leu.2404736","article-title":"Conventional cytogenetics of nodular lymphocyte-predominant Hodgkin's lymphoma","volume":"21","author":"Stamatoullas","year":"2007","journal-title":"Leukemia"},{"key":"10.1016\/j.ajpath.2016.10.007_bib36","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.biocel.2010.09.001","article-title":"CD48: a co-stimulatory receptor of immunity","volume":"43","author":"Elishmereni","year":"2011","journal-title":"Int J Biochem Cell Biol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib37","doi-asserted-by":"crossref","first-page":"259","DOI":"10.2174\/156652412799218903","article-title":"The PD-1\/PD-L1 pathway in human pathology","volume":"12","author":"Saresella","year":"2012","journal-title":"Curr Mol Med"},{"key":"10.1016\/j.ajpath.2016.10.007_bib38","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1097\/01.pas.0000209855.28282.ce","article-title":"Programmed death-1 (PD-1) is a marker of germinal center-associated T cells and angioimmunoblastic T-cell lymphoma","volume":"30","author":"Dorfman","year":"2006","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib39","doi-asserted-by":"crossref","first-page":"3220","DOI":"10.1182\/blood-2007-05-085159","article-title":"PD-1-PD-1 ligand interaction contributes to immunosuppressive microenvironment of Hodgkin lymphoma","volume":"111","author":"Yamamoto","year":"2008","journal-title":"Blood"},{"key":"10.1016\/j.ajpath.2016.10.007_bib40","doi-asserted-by":"crossref","first-page":"5064","DOI":"10.1158\/1078-0432.CCR-13-3271","article-title":"Association of PD-1, PD-1 ligands, and other features of the tumor immune microenvironment with response to anti-PD-1 therapy","volume":"20","author":"Taube","year":"2014","journal-title":"Clin Cancer Res"},{"key":"10.1016\/j.ajpath.2016.10.007_bib41","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1056\/NEJMoa1200690","article-title":"Safety, activity, and immune correlates of anti-PD-1 antibody in cancer","volume":"366","author":"Topalian","year":"2012","journal-title":"N Engl J Med"},{"key":"10.1016\/j.ajpath.2016.10.007_bib42","doi-asserted-by":"crossref","first-page":"2530","DOI":"10.1182\/blood-2011-01-330357","article-title":"Fc gamma receptor IIb on target B cells promotes rituximab internalization and reduces clinical efficacy","volume":"118","author":"Lim","year":"2011","journal-title":"Blood"},{"key":"10.1016\/j.ajpath.2016.10.007_bib43","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1309\/Y8KD32QGRYFN1XQX","article-title":"A double-positive CD4+CD8+ T-cell population is commonly found in nodular lymphocyte predominant Hodgkin lymphoma","volume":"126","author":"Rahemtullah","year":"2006","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib44","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1080\/10428190802308728","article-title":"Beyond the lymphocyte predominant cell: CD4+CD8+ T-cells in nodular lymphocyte predominant Hodgkin lymphoma","volume":"49","author":"Rahemtullah","year":"2008","journal-title":"Leuk Lymphoma"},{"key":"10.1016\/j.ajpath.2016.10.007_bib45","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1002\/(SICI)1521-4141(199808)28:08<2424::AID-IMMU2424>3.0.CO;2-R","article-title":"Amplification of TCRbeta gene rearrangements from micromanipulated single cells: T cells rosetting around Hodgkin and Reed-Sternberg cells in Hodgkin's disease are polyclonal","volume":"28","author":"Roers","year":"1998","journal-title":"Eur J Immunol"},{"key":"10.1016\/j.ajpath.2016.10.007_bib46","doi-asserted-by":"crossref","first-page":"352","DOI":"10.3324\/haematol.2009.016931","article-title":"T-cell\/histiocyte-rich large B-cell lymphoma","volume":"95","author":"Pittaluga","year":"2010","journal-title":"Haematologica"},{"key":"10.1016\/j.ajpath.2016.10.007_bib47","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1634\/theoncologist.11-4-384","article-title":"T-cell\/histiocyte-rich B-cell lymphoma: biology, diagnosis, and management","volume":"11","author":"Abramson","year":"2006","journal-title":"Oncologist"},{"key":"10.1016\/j.ajpath.2016.10.007_bib48","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/s00428-011-1165-z","article-title":"T cell\/histiocyte-rich large B-cell lymphoma: an update on its biology and classification","volume":"459","author":"Tousseyn","year":"2011","journal-title":"Virchows Arch"},{"key":"10.1016\/j.ajpath.2016.10.007_bib49","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1046\/j.1365-2559.2002.01466.x","article-title":"T-cell\/histiocyte-rich large B-cell lymphoma is a disseminated aggressive neoplasm: differential diagnosis from Hodgkin's lymphoma","volume":"41","author":"Fraga","year":"2002","journal-title":"Histopathology"}],"container-title":["The American Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000294401630462X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000294401630462X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T19:31:57Z","timestamp":1658086317000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S000294401630462X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["S000294401630462X"],"URL":"https:\/\/doi.org\/10.1016\/j.ajpath.2016.10.007","relation":{},"ISSN":["0002-9440"],"issn-type":[{"value":"0002-9440","type":"print"}],"subject":[],"published":{"date-parts":[[2017,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting","name":"articletitle","label":"Article Title"},{"value":"The American Journal of Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ajpath.2016.10.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 American Society for Investigative Pathology. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}