{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:06:33Z","timestamp":1789581993895,"version":"build-2803163510"},"reference-count":39,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P42ES010337"],"award-info":[{"award-number":["P42ES010337"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["UL1TR001442"],"award-info":[{"award-number":["UL1TR001442"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ajp.amjpathol.org","clinicalkey.fr","clinicalkey.jp","clinicalkey.com.au","clinicalkey.es","clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The American Journal of Pathology"],"published-print":{"date-parts":[[2024,3]]},"DOI":"10.1016\/j.ajpath.2023.12.005","type":"journal-article","created":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T05:08:35Z","timestamp":1703740115000},"page":"353-368","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["Role of Hepatic Stellate and Liver Sinusoidal Endothelial Cells in a Human Primary Cell Three-Dimensional Model of Nonalcoholic Steatohepatitis"],"prefix":"10.1016","volume":"194","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2573-525X","authenticated-orcid":false,"given":"Philip K.","family":"Tan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Traci","family":"Ostertag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6548-9658","authenticated-orcid":false,"given":"Sara B.","family":"Rosenthal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisy","family":"Chilin-Fuentes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2434-0072","authenticated-orcid":false,"given":"Haylee","family":"Aidnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Linker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey N.","family":"Miner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Brenner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ajpath.2023.12.005_bib1","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/S2468-1253(22)00165-0","article-title":"The prevalence and incidence of NAFLD worldwide: a systematic review and meta-analysis","volume":"7","author":"Riazi","year":"2022","journal-title":"Lancet Gastroenterol Hepatol"},{"key":"10.1016\/j.ajpath.2023.12.005_bib2","article-title":"Non-alcoholic fatty liver disease (NAFLD): summary of NICE guidance","volume":"354","author":"Glen","year":"2016","journal-title":"BMJ"},{"key":"10.1016\/j.ajpath.2023.12.005_bib3","first-page":"14","article-title":"Health and economic burden of nonalcoholic fatty liver disease in the United States and its impact on veterans","volume":"36","author":"Shetty","year":"2019","journal-title":"Fed Pract"},{"key":"10.1016\/j.ajpath.2023.12.005_bib4","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1002\/hep.29367","article-title":"The diagnosis and management of nonalcoholic fatty liver disease: practice guidance from the American Association for the Study of Liver Diseases","volume":"67","author":"Chalasani","year":"2018","journal-title":"Hepatology"},{"key":"10.1016\/j.ajpath.2023.12.005_bib5","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1055\/s-0042-1755316","article-title":"Metabolic injury of hepatocytes promotes progression of NAFLD and AALD","volume":"42","author":"Carvalho-Gontijo","year":"2022","journal-title":"Semin Liver Dis"},{"key":"10.1016\/j.ajpath.2023.12.005_bib6","article-title":"Understanding the cellular interactome of non-alcoholic fatty liver disease","volume":"4","author":"Wallace","year":"2022","journal-title":"JHEP Rep"},{"key":"10.1016\/j.ajpath.2023.12.005_bib7","first-page":"395","article-title":"Emerging roles of liver sinusoidal endothelial cells in nonalcoholic steatohepatitis","volume":"9","author":"Furuta","year":"2020","journal-title":"Biology (Basel)"},{"key":"10.1016\/j.ajpath.2023.12.005_bib8","article-title":"Targeting liver sinusoidal endothelial cells: an attractive therapeutic strategy to control inflammation in nonalcoholic fatty liver disease","volume":"12","author":"Wang","year":"2021","journal-title":"Front Pharmacol"},{"key":"10.1016\/j.ajpath.2023.12.005_bib9","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1007\/s10620-015-3977-1","article-title":"Animal models of nonalcoholic steatohepatitis: eat, delete, and inflame","volume":"61","author":"Ibrahim","year":"2016","journal-title":"Dig Dis Sci"},{"key":"10.1016\/j.ajpath.2023.12.005_bib10","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-01951-7","article-title":"A 3D primary human cell-based in\u00a0vitro model of non-alcoholic steatohepatitis for efficacy testing of clinical drug candidates","volume":"11","author":"Str\u00f6bel","year":"2021","journal-title":"Sci Rep"},{"key":"10.1016\/j.ajpath.2023.12.005_bib11","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.jhep.2018.03.011","article-title":"A simple diet- and chemical-induced murine NASH model with rapid progression of steatohepatitis, fibrosis and liver cancer","volume":"69","author":"Tsuchida","year":"2018","journal-title":"J Hepatol"},{"key":"10.1016\/j.ajpath.2023.12.005_bib12","series-title":"Self assembling cell aggregates and methods of making engineered tissue using the same","author":"Forgacs","year":"2012"},{"key":"10.1016\/j.ajpath.2023.12.005_bib13","series-title":"Self assembling cell aggregates and methods of making engineered tissue using the same","author":"Forgacs","year":"2014"},{"key":"10.1016\/j.ajpath.2023.12.005_bib14","series-title":"Devices, systems, and methods for the fabrication of tissue","author":"Murphy","year":"2015"},{"key":"10.1016\/j.ajpath.2023.12.005_bib15","series-title":"Engineered liver tissues, arrays thereof, and methods of making the same","author":"Shepherd","year":"2015"},{"key":"10.1016\/j.ajpath.2023.12.005_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0158674","article-title":"Bioprinted 3D primary liver tissues allow assessment of organ-level response to clinical drug induced toxicity in\u00a0vitro","volume":"11","author":"Nguyen","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.ajpath.2023.12.005_bib17","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1093\/toxsci\/kfw169","article-title":"Editor's highlight: modeling compound-induced fibrogenesis in\u00a0vitro using three-dimensional bioprinted human liver tissues","volume":"154","author":"Norona","year":"2016","journal-title":"Toxicol Sci"},{"key":"10.1016\/j.ajpath.2023.12.005_bib18","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0208958","article-title":"Bioprinted liver provides early insight into the role of Kupffer cells in TGF-[beta]1 and methotrexate-induced fibrogenesis","volume":"14","author":"Norona","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.ajpath.2023.12.005_bib19","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1089\/aivt.2018.0015","article-title":"Modeling liver biology and the tissue response to injury in bioprinted human liver tissues","volume":"4","author":"Retting","year":"2018","journal-title":"Appl In\u00a0Vitro Toxicol"},{"key":"10.1016\/j.ajpath.2023.12.005_bib20","doi-asserted-by":"crossref","DOI":"10.1088\/1758-5082\/2\/2\/022001","article-title":"Tissue engineering by self-assembly and bio-printing of living cells","volume":"2","author":"Jakab","year":"2010","journal-title":"Biofabrication"},{"key":"10.1016\/j.ajpath.2023.12.005_bib21","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.isci.2018.03.015","article-title":"Bioprinted 3D primary human intestinal tissues model aspects of native physiology and ADME\/Tox functions","volume":"2","author":"Madden","year":"2018","journal-title":"iScience"},{"key":"10.1016\/j.ajpath.2023.12.005_bib22","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1002\/cpt.569","article-title":"3D bioengineered tissues: from advancements in in\u00a0vitro safety to new horizons in disease modeling","volume":"101","author":"Hardwick","year":"2017","journal-title":"Clin Pharmacol Ther"},{"key":"10.1016\/j.ajpath.2023.12.005_bib23","doi-asserted-by":"crossref","first-page":"e1356","DOI":"10.1210\/clinem\/dgab897","article-title":"Fibrogenesis marker PRO-C3 is higher in advanced liver fibrosis and improves in patients undergoing bariatric surgery","volume":"107","author":"Bel Lassen","year":"2022","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.ajpath.2023.12.005_bib24","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3892\/br.2016.681","article-title":"Correlation analysis between four serum biomarkers of liver fibrosis and liver function in infants with cholestasis","volume":"5","author":"Tang","year":"2016","journal-title":"Biomed Rep"},{"key":"10.1016\/j.ajpath.2023.12.005_bib25","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/hepr.12710","article-title":"Classification of patients with non-alcoholic fatty liver disease using rapid immunoassay of serum type IV collagen compared with liver histology and other fibrosis markers","volume":"47","author":"Mizuno","year":"2017","journal-title":"Hepatol Res"},{"key":"10.1016\/j.ajpath.2023.12.005_bib26","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1016\/j.jhep.2021.08.016","article-title":"Comparison of ADAPT, FIB-4 and APRI as non-invasive predictors of liver fibrosis and NASH within the CENTAUR screening population","volume":"75","author":"Nielsen","year":"2021","journal-title":"J Hepatol"},{"key":"10.1016\/j.ajpath.2023.12.005_bib27","article-title":"Determining a healthy reference range and factors potentially influencing PRO-C3 \u2013 a biomarker of liver fibrosis","volume":"3","author":"Erhardtsen","year":"2021","journal-title":"JHEP Rep"},{"key":"10.1016\/j.ajpath.2023.12.005_bib28","doi-asserted-by":"crossref","DOI":"10.1093\/database\/baz046","article-title":"PanglaoDB: a web server for exploration of mouse and human single-cell RNA sequencing data","volume":"2019","author":"Franz\u00e9n","year":"2019","journal-title":"Database (Oxford)"},{"key":"10.1016\/j.ajpath.2023.12.005_bib29","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1002\/hep.31743","article-title":"Heterogeneity of HSCs in a mouse model of NASH","volume":"74","author":"Rosenthal","year":"2021","journal-title":"Hepatology"},{"key":"10.1016\/j.ajpath.2023.12.005_bib30","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/d41573-019-00074-z","article-title":"Trends in clinical success rates and therapeutic focus","volume":"18","author":"Dowden","year":"2019","journal-title":"Nat Rev Drug Discov"},{"key":"10.1016\/j.ajpath.2023.12.005_bib31","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.add3949","article-title":"An autocrine signaling circuit in hepatic stellate cells underlies advanced fibrosis in nonalcoholic steatohepatitis","volume":"15","author":"Wang","year":"2023","journal-title":"Sci Transl Med"},{"key":"10.1016\/j.ajpath.2023.12.005_bib32","doi-asserted-by":"crossref","first-page":"8760","DOI":"10.1038\/s41598-019-45114-1","article-title":"Liver sinusoidal endothelial cells (LSECs) modifications in patients with chronic hepatitis C","volume":"9","author":"Baiocchini","year":"2019","journal-title":"Sci Rep"},{"key":"10.1016\/j.ajpath.2023.12.005_bib33","doi-asserted-by":"crossref","first-page":"929","DOI":"10.3390\/cells9040929","article-title":"The endothelium as a driver of liver fibrosis and regeneration","volume":"9","author":"Lafoz","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.ajpath.2023.12.005_bib34","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.671081","article-title":"Capillarized liver sinusoidal endothelial cells undergo partial endothelial-mesenchymal transition to actively deposit sinusoidal ECM in liver fibrosis","volume":"9","author":"Ruan","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"10.1016\/j.ajpath.2023.12.005_bib35","doi-asserted-by":"crossref","first-page":"G492","DOI":"10.1152\/ajpgi.00428.2016","article-title":"A small population of liver endothelial cells undergoes endothelial-to-mesenchymal transition in response to chronic liver injury","volume":"313","author":"Ribera","year":"2017","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"10.1016\/j.ajpath.2023.12.005_bib36","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.1002\/hep.27376","article-title":"Liver sinusoidal endothelial cells in hepatic fibrosis","volume":"61","author":"DeLeve","year":"2015","journal-title":"Hepatology"},{"key":"10.1016\/j.ajpath.2023.12.005_bib37","doi-asserted-by":"crossref","first-page":"30684","DOI":"10.1074\/jbc.M115.671735","article-title":"Exosome adherence and internalization by hepatic stellate cells triggers sphingosine 1-phosphate-dependent migration","volume":"290","author":"Wang","year":"2015","journal-title":"J Biol Chem"},{"key":"10.1016\/j.ajpath.2023.12.005_bib38","article-title":"Roles of hepatic stellate cells in NAFLD: from the perspective of inflammation and fibrosis","volume":"13","author":"Wang","year":"2022","journal-title":"Front Pharmacol"},{"key":"10.1016\/j.ajpath.2023.12.005_bib39","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.7150\/ijbs.47143","article-title":"Kupffer cells in non-alcoholic fatty liver disease: friend or foe?","volume":"16","author":"Chen","year":"2020","journal-title":"Int J Biol Sci"}],"container-title":["The American Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002944023004844?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002944023004844?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T01:42:11Z","timestamp":1740793331000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0002944023004844"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["S0002944023004844"],"URL":"https:\/\/doi.org\/10.1016\/j.ajpath.2023.12.005","relation":{},"ISSN":["0002-9440"],"issn-type":[{"value":"0002-9440","type":"print"}],"subject":[],"published":{"date-parts":[[2024,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Role of Hepatic Stellate and Liver Sinusoidal Endothelial Cells in a Human Primary Cell Three-Dimensional Model of Nonalcoholic Steatohepatitis","name":"articletitle","label":"Article Title"},{"value":"The American Journal of Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ajpath.2023.12.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 American Society for Investigative Pathology. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}