{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:12:51Z","timestamp":1789495971328,"version":"build-2803163510"},"reference-count":114,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,23]],"date-time":"2026-12-23T00:00:00Z","timestamp":1797984000000},"content-version":"am","delay-in-days":356,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["5U01AA013510-20"],"award-info":[{"award-number":["5U01AA013510-20"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["2R24AA019431-11"],"award-info":[{"award-number":["2R24AA019431-11"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["1R01AA028735"],"award-info":[{"award-number":["1R01AA028735"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007472","name":"University of Kentucky","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007472","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100030643","name":"University of Kentucky Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100030643","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["P51OD011092"],"award-info":[{"award-number":["P51OD011092"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ajp.amjpathol.org","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The American Journal of Pathology"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.ajpath.2025.06.010","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T06:38:20Z","timestamp":1752734300000},"page":"326-343","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chronic Alcohol Consumption Enhances the Differentiation Capacity of Hematopoietic Stem and Progenitor Cells into Osteoclast Precursors"],"prefix":"10.1016","volume":"196","author":[{"given":"Hami","family":"Hemati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1515-1894","authenticated-orcid":false,"given":"Madison B.","family":"Blanton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jude","family":"Koura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupak","family":"Khadka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0380-7219","authenticated-orcid":false,"given":"Kathleen A.","family":"Grant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilhem","family":"Messaoudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ajpath.2025.06.010_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00198-011-1787-7","article-title":"Alcohol and bone: review of dose effects and mechanisms","volume":"23","author":"Maurel","year":"2012","journal-title":"Osteoporos Int"},{"key":"10.1016\/j.ajpath.2025.06.010_bib2","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.3390\/ijerph19031515","article-title":"Alcohol consumption, bone mineral density, and risk of osteoporotic fractures: a dose-response meta-analysis","volume":"19","author":"Godos","year":"2022","journal-title":"Int J Environ Res Public Health"},{"key":"10.1016\/j.ajpath.2025.06.010_bib3","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1097\/01.alc.0000192039.21305.55","article-title":"Alcohol-induced bone loss and deficient bone repair","volume":"29","author":"Chakkalakal","year":"2005","journal-title":"Alcohol Clin Exp Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib4","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.drugalcdep.2019.01.025","article-title":"The effect of alcohol on osteoporosis: a systematic review and meta-analysis","volume":"197","author":"Cheraghi","year":"2019","journal-title":"Drug Alcohol Depend"},{"key":"10.1016\/j.ajpath.2025.06.010_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.bone.2022.116510","article-title":"Altered bone and body composition in children and adolescents with confirmed prenatal alcohol exposure","volume":"164","author":"Young","year":"2022","journal-title":"Bone"},{"key":"10.1016\/j.ajpath.2025.06.010_bib6","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/s41586-019-1105-7","article-title":"Developmental origin, functional maintenance and genetic rescue of osteoclasts","volume":"568","author":"Jacome-Galarza","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.ajpath.2025.06.010_bib7","doi-asserted-by":"crossref","first-page":"127","DOI":"10.11005\/jbm.2023.30.2.127","article-title":"Origin of osteoclasts: osteoclast precursor cells","volume":"30","author":"Tsai","year":"2023","journal-title":"J Bone Metab"},{"key":"10.1016\/j.ajpath.2025.06.010_bib8","doi-asserted-by":"crossref","first-page":"54","DOI":"10.3389\/fcell.2017.00054","article-title":"Regulation of osteoclast growth and fusion by mTOR\/raptor and mTOR\/rictor\/Akt","volume":"5","author":"Tiedemann","year":"2017","journal-title":"Front Cell Dev Biol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib9","series-title":"Osteoimmunology","first-page":"301","article-title":"The role of the immune system in the bone loss of inflammatory arthritis","author":"Goldring","year":"2011"},{"key":"10.1016\/j.ajpath.2025.06.010_bib10","series-title":"Osteoimmunology","first-page":"411","article-title":"Osteoimmunology in the oral cavity (periodontal disease, lesions of endodontic origin and orthodontic tooth movement)","author":"Graves","year":"2011"},{"key":"10.1016\/j.ajpath.2025.06.010_bib11","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.jbo.2016.03.007","article-title":"The role of osteoblasts in bone metastasis","volume":"5","author":"Ottewell","year":"2016","journal-title":"J Bone Oncol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib12","series-title":"Osteoimmunology","first-page":"141","article-title":"The osteoclast: the pioneer of osteoimmunology","author":"Faccio","year":"2011"},{"key":"10.1016\/j.ajpath.2025.06.010_bib13","series-title":"Osteoimmunology","first-page":"7","article-title":"Origins of osteoclasts","author":"Galson","year":"2011"},{"key":"10.1016\/j.ajpath.2025.06.010_bib14","series-title":"Osteoimmunology","first-page":"369","article-title":"The Role of the immune system and bone cells in acute and chronic osteomyelitis","author":"Boyce","year":"2011"},{"key":"10.1016\/j.ajpath.2025.06.010_bib15","doi-asserted-by":"crossref","first-page":"1884","DOI":"10.1016\/j.stemcr.2023.08.001","article-title":"Integrated single cell analysis shows chronic alcohol drinking disrupts monocyte differentiation in the bone marrow","volume":"18","author":"Lewis","year":"2023","journal-title":"Stem Cell Rep"},{"key":"10.1016\/j.ajpath.2025.06.010_bib16","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.alcohol.2020.12.003","article-title":"Effects of graded increases in ethanol consumption on biochemical markers of bone turnover in young adult male cynomolgus macaques","volume":"91","author":"Sattgast","year":"2021","journal-title":"Alcohol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib17","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-65021-4","article-title":"Ethanol consumption in non-human primates alters plasma markers of bone turnover but not tibia architecture","volume":"14","author":"Zhakubayev","year":"2024","journal-title":"Sci Rep"},{"key":"10.1016\/j.ajpath.2025.06.010_bib18","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.bone.2014.10.025","article-title":"Twelve months of voluntary heavy alcohol consumption in male rhesus macaques suppresses intracortical bone remodeling","volume":"71","author":"Gaddini","year":"2015","journal-title":"Bone"},{"key":"10.1016\/j.ajpath.2025.06.010_bib19","doi-asserted-by":"crossref","first-page":"2494","DOI":"10.1111\/acer.14202","article-title":"Voluntary chronic heavy alcohol consumption in male rhesus macaques suppresses cancellous bone formation and increases bone marrow adiposity","volume":"43","author":"Kahler-Quesada","year":"2019","journal-title":"Alcohol Clin Exp Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib20","doi-asserted-by":"crossref","first-page":"117111","DOI":"10.1016\/j.bone.2024.117111","article-title":"Six months of voluntary alcohol consumption in male cynomolgus macaques reduces intracortical bone porosity without altering mineralization or mechanical properties","volume":"185","author":"Kuah","year":"2024","journal-title":"Bone"},{"key":"10.1016\/j.ajpath.2025.06.010_bib21","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.1111\/acer.12547","article-title":"Chronic alcohol self-administration in monkeys shows long-term quantity\/frequency categorical stability","volume":"38","author":"Baker","year":"2014","journal-title":"Alcohol Clin Exp Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib22","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1111\/j.1530-0277.2008.00765.x","article-title":"Drinking typography established by scheduled induction predicts chronic heavy drinking in a monkey model of ethanol self-administration","volume":"32","author":"Grant","year":"2008","journal-title":"Alcohol Clin Exp Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib23","doi-asserted-by":"crossref","first-page":"260","DOI":"10.3389\/fncel.2015.00260","article-title":"An ultrastructural analysis of the effects of ethanol self-administration on the hypothalamic paraventricular nucleus in rhesus macaques","volume":"9","author":"Jimenez","year":"2015","journal-title":"Front Cell Neurosci"},{"key":"10.1016\/j.ajpath.2025.06.010_bib24","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1038\/nprot.2013.092","article-title":"Large-scale gene function analysis with the PANTHER classification system","volume":"8","author":"Mi","year":"2013","journal-title":"Nat Protoc"},{"key":"10.1016\/j.ajpath.2025.06.010_bib25","doi-asserted-by":"crossref","first-page":"D672","DOI":"10.1093\/nar\/gkad1025","article-title":"The Reactome Pathway Knowledgebase 2024","volume":"52","author":"Milacic","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib26","doi-asserted-by":"crossref","first-page":"2473","DOI":"10.1093\/bioinformatics\/btw191","article-title":"flowAI: automatic and interactive anomaly discerning tools for flow cytometry data","volume":"32","author":"Monaco","year":"2016","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ajpath.2025.06.010_bib27","doi-asserted-by":"crossref","first-page":"861","DOI":"10.21105\/joss.00861","article-title":"UMAP: Uniform Manifold Approximation and Projection","volume":"3","author":"McInnes","year":"2018","journal-title":"J Open Source Softw"},{"key":"10.1016\/j.ajpath.2025.06.010_bib28","doi-asserted-by":"crossref","first-page":"3775","DOI":"10.1038\/s41596-021-00550-0","article-title":"Analyzing high-dimensional cytometry data using FlowSOM","volume":"16","author":"Quintelier","year":"2021","journal-title":"Nat Protoc"},{"key":"10.1016\/j.ajpath.2025.06.010_bib29","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.cell.2015.05.047","article-title":"Data-driven phenotypic dissection of AML reveals progenitor-like cells that correlate with prognosis","volume":"162","author":"Levine","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.ajpath.2025.06.010_bib30","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1038\/s41587-019-0336-3","article-title":"Visualizing structure and transitions in high-dimensional biological data","volume":"37","author":"Moon","year":"2019","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib31","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1038\/nprot.2013.032","article-title":"Mouse model of chronic and binge ethanol feeding (the NIAAA model)","volume":"8","author":"Bertola","year":"2013","journal-title":"Nat Protoc"},{"key":"10.1016\/j.ajpath.2025.06.010_bib32","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1182\/blood-2005-03-0860","article-title":"Distinctive and indispensable roles of PU.1 in maintenance of hematopoietic stem cells and their differentiation","volume":"106","author":"Iwasaki","year":"2005","journal-title":"Blood"},{"key":"10.1016\/j.ajpath.2025.06.010_bib33","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.scr.2013.09.006","article-title":"HIF1[alpha] is a regulator of hematopoietic progenitor and stem cell development in hypoxic sites of the mouse embryo","volume":"12","author":"Imanirad","year":"2014","journal-title":"Stem Cell Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib34","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s10616-016-0013-z","article-title":"Down-regulation of Irf8 by Lyz2-cre\/loxP accelerates osteoclast differentiation in vitro","volume":"69","author":"Saito","year":"2017","journal-title":"Cytotechnology"},{"key":"10.1016\/j.ajpath.2025.06.010_bib35","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1161\/CIRCULATIONAHA.121.056019","article-title":"Retention of the NLRP3 inflammasome-primed neutrophils in the bone marrow is essential for myocardial infarction-induced granulopoiesis","volume":"145","author":"Sreejit","year":"2022","journal-title":"Circulation"},{"key":"10.1016\/j.ajpath.2025.06.010_bib36","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1016\/j.stemcr.2024.09.004","article-title":"Interleukin-1[beta] induces trained innate immunity in human hematopoietic progenitor cells in vitro","volume":"19","author":"Flores-Gomez","year":"2024","journal-title":"Stem Cell Reports"},{"key":"10.1016\/j.ajpath.2025.06.010_bib37","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1084\/jem.20161616","article-title":"CXCR1 remodels the vascular niche to promote hematopoietic stem and progenitor cell engraftment","volume":"214","author":"Blaser","year":"2017","journal-title":"J Exp Med"},{"key":"10.1016\/j.ajpath.2025.06.010_bib38","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1182\/blood.2019000495","article-title":"Bone marrow CX3CR1+ mononuclear cells relay a systemic microbiota signal to control hematopoietic progenitors in mice","volume":"134","author":"Lee","year":"2019","journal-title":"Blood"},{"key":"10.1016\/j.ajpath.2025.06.010_bib39","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1749-6632.2012.06559.x","article-title":"G protein-coupled receptor crosstalk and signaling in hematopoietic stem and progenitor cells","volume":"1266","author":"M\u00f6hle","year":"2012","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.ajpath.2025.06.010_bib40","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1089\/scd.2009.0387","article-title":"Bone marrow stem cells contribute to alcohol liver fibrosis in humans","volume":"19","author":"Dalakas","year":"2010","journal-title":"Stem Cells Dev"},{"key":"10.1016\/j.ajpath.2025.06.010_bib41","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1634\/stemcells.21-2-236","article-title":"Interleukin-3 promotes proliferation and differentiation of human hematopoietic stem cells but reduces their repopulation potential in NOD\/SCID mice","volume":"21","author":"Nitsche","year":"2003","journal-title":"Stem Cells"},{"key":"10.1016\/j.ajpath.2025.06.010_bib42","article-title":"IRF8 impacts self-renewal of hematopoietic stem cells by regulating TLR9 signaling pathway of innate immune cells","volume":"8","author":"Li","year":"2021","journal-title":"Adv Sci (Weinh)"},{"key":"10.1016\/j.ajpath.2025.06.010_bib43","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1182\/blood-2014-09-600833","article-title":"IRF8 acts in lineage-committed rather than oligopotent progenitors to control neutrophil vs monocyte production","volume":"125","author":"Ya\u00f1ez","year":"2015","journal-title":"Blood"},{"key":"10.1016\/j.ajpath.2025.06.010_bib44","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/j.immuni.2017.10.021","article-title":"Granulocyte-monocyte progenitors and monocyte-dendritic cell progenitors independently produce functionally distinct monocytes","volume":"47","author":"Ya\u00f1ez","year":"2017","journal-title":"Immunity"},{"key":"10.1016\/j.ajpath.2025.06.010_bib45","doi-asserted-by":"crossref","first-page":"262","DOI":"10.4049\/immunohorizons.1900038","article-title":"Cx3CR1 expression identifies distinct macrophage populations that contribute differentially to inflammation and repair","volume":"3","author":"Burgess","year":"2019","journal-title":"Immunohorizons"},{"key":"10.1016\/j.ajpath.2025.06.010_bib46","doi-asserted-by":"crossref","first-page":"3215","DOI":"10.1038\/s41467-019-11053-8","article-title":"Deletion of a Csf1r enhancer selectively impacts CSF1R expression and development of tissue macrophage populations","volume":"10","author":"Rojo","year":"2019","journal-title":"Nat Commun"},{"key":"10.1016\/j.ajpath.2025.06.010_bib47","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1038\/s12276-020-0484-z","article-title":"The M-CSF receptor in osteoclasts and beyond","volume":"52","author":"Mun","year":"2020","journal-title":"Exp Mol Med"},{"key":"10.1016\/j.ajpath.2025.06.010_bib48","doi-asserted-by":"crossref","DOI":"10.1016\/j.phrs.2022.106566","article-title":"Targeting CSF-1R represents an effective strategy in modulating inflammatory diseases","volume":"187","author":"Xiang","year":"2023","journal-title":"Pharmacol Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib49","doi-asserted-by":"crossref","first-page":"3154","DOI":"10.4049\/jimmunol.2000835","article-title":"A transgenic line that reports CSF1R protein expression provides a definitive marker for the mouse mononuclear phagocyte system","volume":"205","author":"Grabert","year":"2020","journal-title":"J Immunol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib50","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/978-0-387-72009-8_13","article-title":"The enigmatic function of TREM-2 in osteoclastogenesis","volume":"602","author":"Colonna","year":"2007","journal-title":"Adv Exp Med Biol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib51","doi-asserted-by":"crossref","first-page":"2612","DOI":"10.4049\/jimmunol.1102836","article-title":"TREM2 and [beta]-catenin regulate bone homeostasis by controlling the rate of osteoclastogenesis","volume":"188","author":"Otero","year":"2012","journal-title":"J Immunol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib52","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/s41577-019-0178-8","article-title":"Osteoimmunology: evolving concepts in bone-immune interactions in health and disease","volume":"19","author":"Tsukasaki","year":"2019","journal-title":"Nat Rev Immunol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib53","doi-asserted-by":"crossref","first-page":"4592","DOI":"10.1172\/JCI60920","article-title":"Inflammatory arthritis increases mouse osteoclast precursors with myeloid suppressor function","volume":"122","author":"Charles","year":"2012","journal-title":"J Clin Invest"},{"key":"10.1016\/j.ajpath.2025.06.010_bib54","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1359\/JBMR.051007","article-title":"Identification of multiple osteoclast precursor populations in murine bone marrow","volume":"21","author":"Jacquin","year":"2006","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib55","doi-asserted-by":"crossref","first-page":"788","DOI":"10.3389\/fendo.2018.00788","article-title":"Interleukin-6 interweaves the bone marrow microenvironment, bone loss, and multiple myeloma","volume":"9","author":"Harmer","year":"2018","journal-title":"Front Endocrinol (Lausanne)"},{"key":"10.1016\/j.ajpath.2025.06.010_bib56","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.4049\/jimmunol.2100168","article-title":"IL-6 generated from human hematopoietic stem and progenitor cells through TLR4 signaling promotes emergency granulopoiesis by regulating transcription factor expression","volume":"207","author":"Sasaki","year":"2021","journal-title":"J Immunol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-019-0320-5","article-title":"Interleukin-6 signaling regulates hematopoietic stem cell emergence","volume":"51","author":"Tie","year":"2019","journal-title":"Exp Mol Med"},{"key":"10.1016\/j.ajpath.2025.06.010_bib58","doi-asserted-by":"crossref","first-page":"58","DOI":"10.3389\/fimmu.2020.00058","article-title":"Osteoimmunology: a current update of the interplay between bone and the immune system","volume":"11","author":"Guder","year":"2020","journal-title":"Front Immunol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib59","doi-asserted-by":"crossref","first-page":"R131","DOI":"10.1530\/EJE-15-0118","article-title":"Management of endocrine disease: Secondary osteoporosis: pathophysiology and management","volume":"173","author":"Mirza","year":"2015","journal-title":"Eur J Endocrinol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib60","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/S0025-6196(11)62214-3","article-title":"Secondary causes of osteoporosis","volume":"77","author":"Fitzpatrick","year":"2002","journal-title":"Mayo Clin Proc"},{"key":"10.1016\/j.ajpath.2025.06.010_bib61","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0889-8529(02)00062-2","article-title":"Secondary osteoporosis","volume":"32","author":"Stein","year":"2003","journal-title":"Endocrinol Metab Clin North Am"},{"key":"10.1016\/j.ajpath.2025.06.010_bib62","doi-asserted-by":"crossref","first-page":"2382","DOI":"10.3390\/jcm11092382","article-title":"Secondary osteoporosis and metabolic bone diseases","volume":"11","author":"Sobh","year":"2022","journal-title":"J Clin Med"},{"key":"10.1016\/j.ajpath.2025.06.010_bib63","doi-asserted-by":"crossref","DOI":"10.1155\/2016\/3797139","article-title":"The influence of alcohol consumption in conjunction with sex hormone deficiency on Ca\/P ratio in rats","volume":"2016","author":"Lodi","year":"2016","journal-title":"Int J Endocrinol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib64","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/j.beem.2014.07.002","article-title":"Secondary osteoporosis: pathophysiology & diagnosis","volume":"28","author":"Emkey","year":"2014","journal-title":"Best Pract Res Clin Endocrinol Metab"},{"key":"10.1016\/j.ajpath.2025.06.010_bib65","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/nrrheum.2012.93","article-title":"Recognizing and treating secondary osteoporosis","volume":"8","author":"Walker-Bone","year":"2012","journal-title":"Nat Rev Rheumatol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib66","doi-asserted-by":"crossref","first-page":"4443","DOI":"10.1007\/s00018-017-2585-y","article-title":"Cellular and molecular mechanisms of alcohol-induced osteopenia","volume":"74","author":"Luo","year":"2017","journal-title":"Cell Mol Life Sci"},{"key":"10.1016\/j.ajpath.2025.06.010_bib67","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1172\/JCI10483","article-title":"Chronic alcohol ingestion induces osteoclastogenesis and bone loss through IL-6 in mice","volume":"106","author":"Dai","year":"2000","journal-title":"J Clin Invest"},{"key":"10.1016\/j.ajpath.2025.06.010_bib68","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.neuropharm.2017.01.037","article-title":"Alcohol, stress, and glucocorticoids: from risk to dependence and relapse in alcohol use disorders","volume":"122","author":"Blaine","year":"2017","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.ajpath.2025.06.010_bib69","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1111\/j.1530-0277.2010.01298.x","article-title":"The importance of glucocorticoids in alcohol dependence and neurotoxicity","volume":"34","author":"Rose","year":"2010","journal-title":"Alcohol Clin Exp Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib70","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcp.2021.114669","article-title":"Parathyroid hormone and its related peptides in bone metabolism","volume":"192","author":"Chen","year":"2021","journal-title":"Biochem Pharmacol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib71","doi-asserted-by":"crossref","first-page":"S70","DOI":"10.1007\/BF02555094","article-title":"Alcohol and bone","volume":"49 Suppl","author":"Laitinen","year":"1991","journal-title":"Calcif Tissue Int"},{"key":"10.1016\/j.ajpath.2025.06.010_bib72","doi-asserted-by":"crossref","first-page":"44","DOI":"10.3390\/ijms23010044","article-title":"Environmental factors that affect parathyroid hormone and calcitonin levels","volume":"23","author":"Babi\u0107 Leko","year":"2021","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.ajpath.2025.06.010_bib73","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1111\/j.1530-0277.1993.tb00821.x","article-title":"Alcohol-induced bone disease: relationship to age and parathyroid hormone levels","volume":"17","author":"Bikle","year":"1993","journal-title":"Alcohol Clin Exp Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib74","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.psychres.2016.10.051","article-title":"Vitamin D and alcohol: a review of the current literature","volume":"248","author":"Tardelli","year":"2017","journal-title":"Psychiatry Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib75","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1093\/alcalc\/agq098","article-title":"Vitamin D and nutritional status are related to bone fractures in alcoholics","volume":"46","author":"Gonz\u00e1lez-Reimers","year":"2011","journal-title":"Alcohol Alcohol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib76","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0741-8329(00)00115-4","article-title":"Osteopenia assessed by body composition analysis is related to malnutrition in alcoholic patients","volume":"22","author":"Santolaria","year":"2000","journal-title":"Alcohol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib77","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/j.bbamcr.2010.11.002","article-title":"Calcium signaling in osteoclasts","volume":"1813","author":"Hwang","year":"2011","journal-title":"Biochim Biophys Acta"},{"key":"10.1016\/j.ajpath.2025.06.010_bib78","first-page":"190","article-title":"Alcohol\u2019s harmful effects on bone","volume":"22","author":"Sampson","year":"1998","journal-title":"Alcohol Health Res World"},{"key":"10.1016\/j.ajpath.2025.06.010_bib79","first-page":"338","article-title":"Concurrent gut transcriptome and microbiota profiling following chronic ethanol consumption in nonhuman primates","volume":"9","author":"Barr","year":"2018","journal-title":"Gut Microbes"},{"key":"10.1016\/j.ajpath.2025.06.010_bib80","doi-asserted-by":"crossref","first-page":"794","DOI":"10.3389\/fendo.2018.00794","article-title":"Osteocalcin\u2014a versatile bone-derived hormone","volume":"9","author":"Moser","year":"2018","journal-title":"Front Endocrinol (Lausanne)"},{"key":"10.1016\/j.ajpath.2025.06.010_bib81","doi-asserted-by":"crossref","first-page":"3425","DOI":"10.1007\/s00213-014-3590-6","article-title":"Adrenal steroid hormones and ethanol self-administration in male rhesus macaques","volume":"231","author":"Helms","year":"2014","journal-title":"Psychopharmacology (Berl)"},{"key":"10.1016\/j.ajpath.2025.06.010_bib82","article-title":"Dose-response effects of alcohol on biochemical markers of bone turnover in non-human primates: effects of species, sex and age of onset of drinking","volume":"16","author":"Benton","year":"2022","journal-title":"Bone Rep"},{"key":"10.1016\/j.ajpath.2025.06.010_bib83","doi-asserted-by":"crossref","first-page":"159","DOI":"10.35946\/arcr.v37.2.01","article-title":"Alcohol\u2019s effect on host defense","volume":"37","author":"Szabo","year":"2015","journal-title":"Alcohol Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib84","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/j.ebiom.2019.04.027","article-title":"Chronic heavy drinking drives distinct transcriptional and epigenetic changes in splenic macrophages","volume":"43","author":"Sureshchandra","year":"2019","journal-title":"EBioMedicine"},{"key":"10.1016\/j.ajpath.2025.06.010_bib85","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0159295","article-title":"Transcriptome profiling reveals disruption of innate immunity in chronic heavy ethanol consuming female rhesus macaques","volume":"11","author":"Sureshchandra","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.ajpath.2025.06.010_bib86","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.3390\/ijms20061272","article-title":"Causes and mechanisms of hematopoietic stem cell aging","volume":"20","author":"Lee","year":"2019","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.ajpath.2025.06.010_bib87","doi-asserted-by":"crossref","first-page":"2672","DOI":"10.1182\/blood-2024-211006","article-title":"Combinatorial effect of chronic alcohol drinking and age perpetuates myeloid-biased hematopoiesis via hematopoietic stem cell senescence","volume":"144 Suppl 1","author":"Yuda","year":"2024","journal-title":"Blood"},{"issue":"Suppl 1","key":"10.1016\/j.ajpath.2025.06.010_bib88","doi-asserted-by":"crossref","first-page":"2676","DOI":"10.1182\/blood-2023-190008","article-title":"Chronic alcohol exposure causes myeloid-biased hematopoiesis via Double-strand RNA sensors and Type 1 interferon response","volume":"142","author":"Bea","year":"2023","journal-title":"Blood"},{"key":"10.1016\/j.ajpath.2025.06.010_bib89","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.abb.2008.03.018","article-title":"Functions of RANKL\/RANK\/OPG in bone modeling and remodeling","volume":"473","author":"Boyce","year":"2008","journal-title":"Arch Biochem Biophys"},{"key":"10.1016\/j.ajpath.2025.06.010_bib90","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.stem.2019.05.019","article-title":"TNF-[alpha] coordinates hematopoietic stem cell survival and myeloid regeneration","volume":"25","author":"Yamashita","year":"2019","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.ajpath.2025.06.010_bib91","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21207717","article-title":"Osteoclast fusion: physiological regulation of multinucleation through heterogeneity-potential implications for drug sensitivity","volume":"21","author":"S\u00f8e","year":"2020","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.ajpath.2025.06.010_bib92","doi-asserted-by":"crossref","first-page":"236","DOI":"10.3389\/fendo.2019.00236","article-title":"Updates on osteoimmunology: what\u2019s new on the cross-talk between bone and immune system","volume":"10","author":"Ponzetti","year":"2019","journal-title":"Front Endocrinol (Lausanne)"},{"key":"10.1016\/j.ajpath.2025.06.010_bib93","doi-asserted-by":"crossref","DOI":"10.1172\/JCI140214","article-title":"Bone marrow adipogenic lineage precursors promote osteoclastogenesis in bone remodeling and pathologic bone loss","volume":"131","author":"Yu","year":"2021","journal-title":"J Clin Invest"},{"key":"10.1016\/j.ajpath.2025.06.010_bib94","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.jot.2024.06.010","article-title":"Osteostaticytes: a novel osteoclast subset couples bone resorption and bone formation","volume":"47","author":"Wei","year":"2024","journal-title":"J Orthop Translat"},{"key":"10.1016\/j.ajpath.2025.06.010_bib95","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1038\/s42255-020-00318-y","article-title":"Stepwise cell fate decision pathways during osteoclastogenesis at single-cell resolution","volume":"2","author":"Tsukasaki","year":"2020","journal-title":"Nat Metab"},{"key":"10.1016\/j.ajpath.2025.06.010_bib96","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s41232-022-00213-x","article-title":"Osteoclast biology in the single-cell era","volume":"42","author":"Tsukasaki","year":"2022","journal-title":"Inflamm Regen"},{"key":"10.1016\/j.ajpath.2025.06.010_bib97","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.3390\/ph3051394","article-title":"Effects of NSAIDs on differentiation and function of human and murine osteoclasts\u2014crucial \u2018human osteoclastology.","volume":"3","author":"Kotake","year":"2010","journal-title":"Pharmaceuticals (Basel)"},{"key":"10.1016\/j.ajpath.2025.06.010_bib98","series-title":"Osteoporosis Pathophysiology and Clinical Management","author":"Wein","year":"2020"},{"key":"10.1016\/j.ajpath.2025.06.010_bib99","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1196\/annals.1346.047","article-title":"Interactive effect of interleukin-6 and prostaglandin E2 on osteoclastogenesis via the OPG\/RANKL\/RANK system","volume":"1068","author":"Liu","year":"2006","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.ajpath.2025.06.010_bib100","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1038\/s41420-018-0134-6","article-title":"Epigenetic regulation of NfatC1 transcription and osteoclastogenesis by nicotinamide phosphoribosyl transferase in the pathogenesis of arthritis","volume":"5","author":"Li","year":"2019","journal-title":"Cell Death Discov"},{"key":"10.1016\/j.ajpath.2025.06.010_bib101","doi-asserted-by":"crossref","DOI":"10.1016\/j.redox.2023.102711","article-title":"NFATc1-mediated expression of SLC7A11 drives sensitivity to TXNRD1 inhibitors in osteoclast precursors","volume":"63","author":"Zhong","year":"2023","journal-title":"Redox Biol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib102","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1515\/bc.2011.015","article-title":"Pumping lipids with P4-ATPases","volume":"392","author":"L\u00f3pez-Marqu\u00e9s","year":"2011","journal-title":"Biol Chem"},{"key":"10.1016\/j.ajpath.2025.06.010_bib103","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.644986","article-title":"Membrane transport proteins in osteoclasts: the ins and outs","volume":"9","author":"Ribet","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib104","doi-asserted-by":"crossref","first-page":"6836","DOI":"10.1038\/srep06836","article-title":"Identification of a mammalian vesicular polyamine transporter","volume":"4","author":"Hiasa","year":"2014","journal-title":"Sci Rep"},{"key":"10.1016\/j.ajpath.2025.06.010_bib105","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s13023-015-0235-8","article-title":"Impaired osteoblast and osteoclast function characterize the osteoporosis of Snyder-Robinson syndrome","volume":"10","author":"Albert","year":"2015","journal-title":"Orphanet J Rare Dis"},{"key":"10.1016\/j.ajpath.2025.06.010_bib106","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/nature12984","article-title":"The bone marrow niche for haematopoietic stem cells","volume":"505","author":"Morrison","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.ajpath.2025.06.010_bib107","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1084\/jem.20101890","article-title":"Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization","volume":"208","author":"Miyamoto","year":"2011","journal-title":"J Exp Med"},{"key":"10.1016\/j.ajpath.2025.06.010_bib108","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1002\/hep.1840130211","article-title":"Circulating tumor necrosis factor, interleukin-1 and interleukin-6 concentrations in chronic alcoholic patients","volume":"13","author":"Khoruts","year":"1991","journal-title":"Hepatology"},{"key":"10.1016\/j.ajpath.2025.06.010_bib109","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1093\/alcalc\/agh059","article-title":"Alcohol misuse increases serum antibodies to oxidized LDL and C-reactive protein","volume":"39","author":"Alho","year":"2004","journal-title":"Alcohol Alcohol"},{"key":"10.1016\/j.ajpath.2025.06.010_bib110","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1111\/j.1530-0277.2010.01364.x","article-title":"Individual differences in hyperlipidemia and vitamin E status in response to chronic alcohol self-administration in cynomolgus monkeys","volume":"35","author":"Lebold","year":"2011","journal-title":"Alcohol Clin Exp Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib111","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s13075-015-0563-z","article-title":"Role of C-reactive protein in osteoclastogenesis in rheumatoid arthritis","volume":"17","author":"Kim","year":"2015","journal-title":"Arthritis Res Ther"},{"key":"10.1016\/j.ajpath.2025.06.010_bib112","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/s41413-022-00206-z","article-title":"Specific inflammatory osteoclast precursors induced during chronic inflammation give rise to highly active osteoclasts associated with inflammatory bone loss","volume":"10","author":"Meirow","year":"2022","journal-title":"Bone Res"},{"key":"10.1016\/j.ajpath.2025.06.010_bib113","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/nature04247","article-title":"Stem cell engraftment at the endosteal niche is specified by the calcium-sensing receptor","volume":"439","author":"Adams","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.ajpath.2025.06.010_bib114","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/nature25154","article-title":"Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells","volume":"553","author":"Garaycoechea","year":"2018","journal-title":"Nature"}],"container-title":["The American Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002944025002445?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002944025002445?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T10:21:27Z","timestamp":1773051687000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0002944025002445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":114,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["S0002944025002445"],"URL":"https:\/\/doi.org\/10.1016\/j.ajpath.2025.06.010","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.02.05.636743","asserted-by":"object"}]},"ISSN":["0002-9440"],"issn-type":[{"value":"0002-9440","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Chronic Alcohol Consumption Enhances the Differentiation Capacity of Hematopoietic Stem and Progenitor Cells into Osteoclast Precursors","name":"articletitle","label":"Article Title"},{"value":"The American Journal of Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ajpath.2025.06.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 American Society for Investigative Pathology. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}