{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T09:11:19Z","timestamp":1787821879781,"version":"build-2784847793"},"reference-count":42,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["amjmed.com","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The American Journal of Medicine"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.amjmed.2025.09.031","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:36:41Z","timestamp":1760661401000},"page":"350-360","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["TRAKJAK: a complete blood count-based prediction of polycythemia vera at initial erythrocytosis workup to reduce financial and ecological costs"],"prefix":"10.1016","volume":"139","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0254-3394","authenticated-orcid":false,"given":"Etienne","family":"Rivi\u00e8re","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Mansier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Guy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Benoit","family":"Corcuff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Rivi\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Contis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Duffau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyril Le","family":"Moal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mence","family":"Mediavilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Sibon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estibaliz","family":"Lazaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9","family":"James","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Viallard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.amjmed.2025.09.031_bib0001","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1111\/j.1365-2141.1995.tb08411.x","article-title":"Interpretation of measured red cell mass and plasma volume in adults: expert Panel on Radionuclides of the International Council for Standardization in Haematology","volume":"89","author":"Pearson","year":"1995","journal-title":"Br J Haematol"},{"issue":"1","key":"10.1016\/j.amjmed.2025.09.031_bib0002","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/j.1600-0609.1984.tb02212.x","article-title":"Interpretation of measured red cell mass and plasma volume in males with elevated venous PCV values","volume":"33","author":"Pearson","year":"1984","journal-title":"Scandinav J Haematol"},{"issue":"2","key":"10.1016\/j.amjmed.2025.09.031_bib0003","doi-asserted-by":"crossref","first-page":"e107","DOI":"10.1111\/bjh.16747","article-title":"Can absolute polycythaemia be identified without measurement of the red cell mass?","volume":"190","author":"Grenier","year":"2020","journal-title":"Br J Haematol"},{"issue":"2","key":"10.1016\/j.amjmed.2025.09.031_bib0004","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1001\/jama.2024.20377","article-title":"Diagnosis and treatment of Polycythemia Vera: a review","volume":"333","author":"Tremblay","year":"2025","journal-title":"JAMA"},{"issue":"7037","key":"10.1016\/j.amjmed.2025.09.031_bib0005","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1038\/nature03546","article-title":"A unique clonal JAK2 mutation leading to constitutive signalling causes polycythaemia vera","volume":"434","author":"James","year":"2005","journal-title":"Nature"},{"issue":"5","key":"10.1016\/j.amjmed.2025.09.031_bib0006","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1056\/NEJMoa065202","article-title":"JAK2 Exon 12 mutations in polycythemia vera and idiopathic erythrocytosis","volume":"356","author":"Scott","year":"2007","journal-title":"N Engl J Med"},{"issue":"25","key":"10.1016\/j.amjmed.2025.09.031_bib0007","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1056\/NEJMoa1311347","article-title":"Somatic mutations of calreticulin in myeloproliferative neoplasms","volume":"369","author":"Klampfl","year":"2013","journal-title":"N Engl J Med"},{"issue":"25","key":"10.1016\/j.amjmed.2025.09.031_bib0008","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1056\/NEJMoa1312542","article-title":"Somatic CALR mutations in myeloproliferative neoplasms with nonmutated JAK2","volume":"369","author":"Nangalia","year":"2013","journal-title":"N Engl J Med"},{"issue":"7","key":"10.1016\/j.amjmed.2025.09.031_bib0009","doi-asserted-by":"crossref","first-page":"e270","DOI":"10.1371\/journal.pmed.0030270","article-title":"MPLW515L Is a novel somatic activating mutation in myelofibrosis with myeloid metaplasia","volume":"3","author":"Pikman","year":"2006","journal-title":"PLOS Medicine"},{"issue":"9","key":"10.1016\/j.amjmed.2025.09.031_bib0010","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1002\/ajh.27002","article-title":"Polycythemia vera: 2024 update on diagnosis, risk-stratification, and management","volume":"98","author":"Tefferi","year":"2023","journal-title":"Am J Hematol"},{"issue":"1","key":"10.1016\/j.amjmed.2025.09.031_bib0011","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1046\/j.1365-2141.2002.03386.x","article-title":"Serum erythropoietin values in erythrocytoses and in primary thrombocythaemia","volume":"117","author":"Messinezy","year":"2002","journal-title":"Br J Haematol"},{"issue":"10","key":"10.1016\/j.amjmed.2025.09.031_bib0012","first-page":"1194","article-title":"Diagnostic value of serum erythropoietin level in patients with absolute erythrocytosis","volume":"89","author":"Mossuz","year":"2004","journal-title":"Haematologica"},{"issue":"3","key":"10.1016\/j.amjmed.2025.09.031_bib0013","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/bjh.13047","article-title":"The role of serum erythropoietin level and JAK2 V617F allele burden in the diagnosis of polycythaemia vera","volume":"167","author":"Ancochea","year":"2014","journal-title":"Br J Haematol"},{"key":"10.1016\/j.amjmed.2025.09.031_bib0014","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcmd.2019.102355","article-title":"The role of a low erythropoietin level for the polycythemia vera diagnosis","volume":"80","author":"Lupak","year":"2020","journal-title":"Blood Cells Mol Dis"},{"issue":"3","key":"10.1016\/j.amjmed.2025.09.031_bib0015","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1111\/bjh.17848","article-title":"Revisiting diagnostic performances of serum erythropo\u00efetin level and JAK2 mutation for polycythemias: analysis of a cohort of 1090 patients with red cell mass measurement","volume":"196","author":"Maslah","year":"2022","journal-title":"Br J Haematol"},{"issue":"2","key":"10.1016\/j.amjmed.2025.09.031_bib0016","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1002\/cam4.4958","article-title":"Clinical features of acquired erythrocytosis: low levels of serum erythropoietin in a subset of non-neoplastic erythrocytosis patients","volume":"12","author":"Mori","year":"2023","journal-title":"Cancer Med"},{"key":"10.1016\/j.amjmed.2025.09.031_bib0017","doi-asserted-by":"crossref","unstructured":"Chin-Yee B., Cheong I., Matyashin M., et al. Serum erythropoietin levels in 696 patients investigated for erythrocytosis with JAK2 mutation analysis. doi:10.1002\/ajh.26471","DOI":"10.1002\/ajh.26471"},{"key":"10.1016\/j.amjmed.2025.09.031_bib0018","doi-asserted-by":"crossref","DOI":"10.1016\/j.greeac.2023.100051","article-title":"Carbon footprint of the analytical laboratory and the three-dimensional approach to its reduction","volume":"4","author":"Nowak","year":"2023","journal-title":"Green Anal Chem"},{"key":"10.1016\/j.amjmed.2025.09.031_bib0019","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.biochi.2024.01.013","article-title":"Amplifying PCR productivity and environmental sustainability through shortened cycling protocols","volume":"221","author":"Pedlar","year":"2024","journal-title":"Biochimie"},{"issue":"1","key":"10.1016\/j.amjmed.2025.09.031_bib0020","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pclm.0000561","article-title":"The direct emissions related to Global Warming Potential of different types of diagnostic tests at different phases of the COVID pandemic: a climate-focused life-cycle assessment","volume":"4","author":"Courdier","year":"2025","journal-title":"PLOS Climate"},{"issue":"5","key":"10.1016\/j.amjmed.2025.09.031_bib0021","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.jmoldx.2011.05.007","article-title":"Uses and abuses of JAK2 and MPL mutation tests in myeloproliferative neoplasms","volume":"13","author":"Tefferi","year":"2011","journal-title":"J Molecul Diagnost"},{"issue":"20","key":"10.1016\/j.amjmed.2025.09.031_bib0022","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1182\/blood-2016-03-643544","article-title":"The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia","volume":"127","author":"Arber","year":"2016","journal-title":"Blood"},{"issue":"4","key":"10.1016\/j.amjmed.2025.09.031_bib0023","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1182\/blood.2018834044","article-title":"How I treat polycythemia vera","volume":"134","author":"Spivak","year":"2019","journal-title":"Blood"},{"issue":"1","key":"10.1016\/j.amjmed.2025.09.031_bib0024","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1159\/000358580","article-title":"Changing concepts of diagnostic criteria of myeloproliferative disorders and the molecular etiology and classification of myeloproliferative neoplasms: from Dameshek 1950 to Vainchenker 2005 and beyond","volume":"133","author":"Michiels","year":"2014","journal-title":"Acta Haematol"},{"issue":"3","key":"10.1016\/j.amjmed.2025.09.031_bib0025","doi-asserted-by":"crossref","first-page":"e95","DOI":"10.3324\/haematol.2018.215582","article-title":"Masked polycythemia vera: analysis of a single center cohort of 2480 red cell masses","volume":"105","author":"Maslah","year":"2020","journal-title":"Haematologica"},{"issue":"1","key":"10.1016\/j.amjmed.2025.09.031_bib0026","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1038\/s41408-024-01176-7","article-title":"Neutrophil-to-lymphocyte ratio as a prognostic indicator of mortality in Polycythemia Vera: insights from a prospective cohort analysis","volume":"14","author":"Barbui","year":"2024","journal-title":"Blood Cancer J"},{"issue":"6","key":"10.1016\/j.amjmed.2025.09.031_bib0027","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1182\/blood-2006-01-013540","article-title":"The JAK2-V617F mutation is frequently present at diagnosis in patients with essential thrombocythemia and polycythemia vera","volume":"108","author":"Lippert","year":"2006","journal-title":"Blood"},{"issue":"6","key":"10.1016\/j.amjmed.2025.09.031_bib0028","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1111\/j.1600-0609.2007.00960.x","article-title":"The JAK2 V617F allele burden in essential thrombocythemia, polycythemia vera and primary myelofibrosis\u2013impact on disease phenotype","volume":"79","author":"Larsen","year":"2007","journal-title":"Eur J Haematol"},{"issue":"3","key":"10.1016\/j.amjmed.2025.09.031_bib0029","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.jmoldx.2010.12.002","article-title":"Nested high-resolution melting curve analysis a highly sensitive, reliable, and simple method for detection of JAK2 exon 12 mutations\u2013clinical relevance in the monitoring of polycythemia","volume":"13","author":"Carillo","year":"2011","journal-title":"J Mol Diagn"},{"issue":"11\u201312","key":"10.1016\/j.amjmed.2025.09.031_bib0030","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1007\/s00508-022-02027-w","article-title":"High platelet-to-lymphocyte ratio may differentiate polycythemia vera from secondary polycythemia","volume":"134","author":"Kre\u010dak","year":"2022","journal-title":"Wien Klin Wochenschr"},{"issue":"15","key":"10.1016\/j.amjmed.2025.09.031_bib0031","doi-asserted-by":"crossref","first-page":"4459","DOI":"10.3390\/jcm13154459","article-title":"Systemic inflammatory index in polycythemia vera and its prognostic implications","volume":"13","author":"Krecak","year":"2024","journal-title":"J Clin Med"},{"issue":"8","key":"10.1016\/j.amjmed.2025.09.031_bib0032","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1007\/s11606-022-07963-x","article-title":"A rational approach to JAK2 mutation testing in patients with elevated hemoglobin: results from the JAK2 prediction cohort (JAKPOT) study","volume":"38","author":"Chin-Yee","year":"2023","journal-title":"J Gen Intern Med"},{"issue":"4","key":"10.1016\/j.amjmed.2025.09.031_bib0033","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.clml.2020.11.002","article-title":"Diagnostic performance of erythropoietin levels in Polycythemia Vera: experience at a comprehensive cancer center","volume":"21","author":"Davila-Gonzalez","year":"2021","journal-title":"Clin Lymphoma Myeloma Leuk"},{"issue":"2","key":"10.1016\/j.amjmed.2025.09.031_bib0034","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/ajh.23617","article-title":"Masked polycythemia vera diagnosed according to WHO and BCSH classification","volume":"89","author":"Barbui","year":"2014","journal-title":"Am J Hematol"},{"issue":"6","key":"10.1016\/j.amjmed.2025.09.031_bib0035","first-page":"1989","article-title":"Role of red cell mass evaluation in myeloproliferative neoplasms with splanchnic vein thrombosis and normal hemoglobin value: a study of the France Intergroupe des Syndromes myeloprolif\u00e9ratifs","volume":"109","author":"Galtier","year":"2024","journal-title":"Haematologica"},{"issue":"2","key":"10.1016\/j.amjmed.2025.09.031_bib0036","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/ijlh.12967","article-title":"A CBC algorithm combined with immature platelet fraction is able to identify JAK2 V617F mutation-positive polycythaemia vera patients","volume":"41","author":"Johnson","year":"2019","journal-title":"Int J Lab Hematol"},{"issue":"2","key":"10.1016\/j.amjmed.2025.09.031_bib0037","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1136\/jclinpath-2018-205527","article-title":"JAK2-tree: a simple CBC-based decision rule to guide appropriate JAK2 V617F mutation testing","volume":"72","author":"Mahe","year":"2019","journal-title":"J Clin Pathol"},{"issue":"1","key":"10.1016\/j.amjmed.2025.09.031_bib0038","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/s41598-020-80459-y","article-title":"Development and validation of a sequential two-step algorithm for the screening of individuals with potential polycythaemia vera","volume":"11","author":"Piris-Villaespesa","year":"2021","journal-title":"Sci Rep"},{"issue":"1","key":"10.1016\/j.amjmed.2025.09.031_bib0039","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jmoldx.2024.10.003","article-title":"Analysis of molecular testing for suspected myeloproliferative neoplasm at a hybrid community-Academic Health system","volume":"27","author":"Stone","year":"2025","journal-title":"J Mol Diagn"},{"issue":"8","key":"10.1016\/j.amjmed.2025.09.031_bib0040","doi-asserted-by":"crossref","first-page":"377","DOI":"10.5694\/mja2.50583","article-title":"The carbon footprint of pathology testing","volume":"212","author":"McAlister","year":"2020","journal-title":"Med J Aust"},{"issue":"4","key":"10.1016\/j.amjmed.2025.09.031_bib0041","doi-asserted-by":"crossref","first-page":"e285","DOI":"10.1055\/a-2165-1249","article-title":"Impact of COVID-19 Pandemic on temporal trends of hemostasis test in France: a retrospective analysis of 9 years of national health data","volume":"7","author":"Bentounes","year":"2023","journal-title":"TH Open"},{"key":"10.1016\/j.amjmed.2025.09.031_bib42","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1111\/joim.13019","article-title":"Incidence of myeloproliferative neoplasms \u2013 trends by subgroup and age in a population-based study in Sweden","volume":"287","author":"Hultcrantz","year":"2020","journal-title":"J Intern Med"}],"container-title":["The American Journal of Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000293432500703X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000293432500703X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T04:37:52Z","timestamp":1771216672000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S000293432500703X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["S000293432500703X"],"URL":"https:\/\/doi.org\/10.1016\/j.amjmed.2025.09.031","relation":{},"ISSN":["0002-9343"],"issn-type":[{"value":"0002-9343","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"TRAKJAK: a complete blood count-based prediction of polycythemia vera at initial erythrocytosis workup to reduce financial and ecological costs","name":"articletitle","label":"Article Title"},{"value":"The American Journal of Medicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.amjmed.2025.09.031","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}