{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T14:30:58Z","timestamp":1791469858241,"version":"4.3.4"},"reference-count":41,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,8,22]],"date-time":"2020-08-22T00:00:00Z","timestamp":1598054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["American Journal of Otolaryngology"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1016\/j.amjoto.2020.102681","type":"journal-article","created":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T12:20:59Z","timestamp":1597321259000},"page":"102681","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"title":["Association of pepsin and DNA damage in laryngopharyngeal reflux-related vocal fold polyps"],"prefix":"10.1016","volume":"41","author":[{"given":"Yuan-Feng","family":"Dai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Jie","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Qun","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ze-Hong","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Ping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.amjoto.2020.102681_bb0005","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.jvoice.2003.07.003","article-title":"Vocal fold nodule vs. vocal fold polyp: answer from surgical pathologist and voice pathologist point of view","volume":"18","author":"Wallis","year":"2004","journal-title":"J Voice"},{"key":"10.1016\/j.amjoto.2020.102681_bb0010","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1002\/lary.23743","article-title":"Expression of hypoxia inducible factor-1\u03b1 and vascular endothelia growth factor in vocal polyps","volume":"123","author":"Fang","year":"2013","journal-title":"Laryngoscope"},{"key":"10.1016\/j.amjoto.2020.102681_bb0015","doi-asserted-by":"crossref","first-page":"E329","DOI":"10.1002\/lary.27932","article-title":"Association between laryngopharyngeal reflux and benign vocal folds lesions: a systematic review","volume":"129","author":"Lechien","year":"2019","journal-title":"Laryngoscope"},{"key":"10.1016\/j.amjoto.2020.102681_bb0020","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.jvoice.2017.03.015","article-title":"Laryngopharyngeal reflux and voice disorders: a multifactorial model of etiology and pathophysiology","volume":"31","author":"Lechien","year":"2017","journal-title":"J Voice"},{"key":"10.1016\/j.amjoto.2020.102681_bb0025","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1177\/000348940811700911","article-title":"Mucin gene expression in human laryngeal epithelia: effect of laryngopharyngeal reflux","volume":"117","author":"Samuels","year":"2008","journal-title":"Ann Otol Rhinol Laryngol"},{"key":"10.1016\/j.amjoto.2020.102681_bb0030","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1177\/000348940511401204","article-title":"Laryngeal epithelial defenses against laryngopharyngeal reflux: investigations of E-cadherin, carbonic anhydrase isoenzyme III, and pepsin","volume":"114","author":"Gill","year":"2005","journal-title":"Ann Otol Rhinol Laryngol"},{"issue":"8","key":"10.1016\/j.amjoto.2020.102681_bb0035","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1007\/s00405-007-0568-6","article-title":"E-cadherin but not beta-catenin expression is decreased in laryngeal biopsies from patients with laryngopharyngeal reflux","volume":"265","author":"Mayr","year":"2008","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"10.1016\/j.amjoto.2020.102681_bb0040","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1002\/lary.27591","article-title":"Clinical outcomes of laryngopharyngeal reflux treatment: a systematic review and meta-analysis","volume":"129","author":"Lechien","year":"2019","journal-title":"Laryngoscope"},{"key":"10.1016\/j.amjoto.2020.102681_bb0045","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1067\/mhn.2000.109935","article-title":"Prevalence of reflux in 113 consecutive patients with laryngeal and voice disorders","volume":"123","author":"Koufman","year":"2000","journal-title":"Otolaryngol Head Neck Surg"},{"key":"10.1016\/j.amjoto.2020.102681_bb0050","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1007\/s00405-011-1572-4","article-title":"Double probe pH-monitoring findings in patients with benign lesions of the true vocal folds: comparison with typical GERD and the effect of smoking","volume":"268","author":"Beltsis","year":"2011","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"10.1016\/j.amjoto.2020.102681_bb0055","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1177\/0194599816676471","article-title":"Association between laryngeal pepsin levels and the presence of vocal fold polyps","volume":"156","author":"Wang","year":"2017","journal-title":"Otolaryngol Head Neck Surg"},{"key":"10.1016\/j.amjoto.2020.102681_bb0060","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1177\/000348941011900310","article-title":"Pepsin as a marker of extraesophageal reflux","volume":"119","author":"Samuels","year":"2010","journal-title":"Ann Otol Rhinol Laryngol"},{"issue":"3","key":"10.1016\/j.amjoto.2020.102681_bb0065","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.otohns.2009.05.033","article-title":"The significance of laryngopharyngeal reflux in benign vocal mucosal lesions","volume":"141","author":"Chung","year":"2009","journal-title":"Otolaryngol Head Neck Surg"},{"key":"10.1016\/j.amjoto.2020.102681_bb0070","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1007\/s11883-014-0452-y","article-title":"8-Hydroxy-2\u2032-deoxyguanosine and cardiovascular disease: a systematic review","volume":"16","author":"Kroese","year":"2014","journal-title":"Curr Atheroscler Rep"},{"key":"10.1016\/j.amjoto.2020.102681_bb0075","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1111\/resp.12378","article-title":"Feasibility of 8-OHdG formation and hOGG1 induction in PBMCs for assessing oxidative DNA damage in the lung of COPD patients","volume":"19","author":"Yang","year":"2014","journal-title":"Respirology"},{"key":"10.1016\/j.amjoto.2020.102681_bb0080","doi-asserted-by":"crossref","first-page":"G863","DOI":"10.1152\/ajpgi.00321.2013","article-title":"Role of intracellular calcium and NADPH oxidase NOX5-S in acid-induced DNA damage in Barrett\u2019s cells and Barrett\u2019s esophageal adenocarcinoma cells","volume":"306","author":"Li","year":"2014","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"10.1016\/j.amjoto.2020.102681_bb0085","first-page":"1161","article-title":"Prevention of DNA damage in Barrett\u2019s esophageal cells exposed to acidic bile salts","volume":"37","author":"Bhardwaj","year":"2016","journal-title":"Carcinogenesis"},{"key":"10.1016\/j.amjoto.2020.102681_bb0090","doi-asserted-by":"crossref","first-page":"9956","DOI":"10.1038\/s41598-017-09620-4","article-title":"Activation of NADPH oxidases leads to DNA damage in esophageal cells","volume":"7","author":"Bhardwaj","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.amjoto.2020.102681_bb0095","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/0963689720908198","article-title":"Establishment of immortalized laryngeal epithelial cells transfected with Bmi1","volume":"29","author":"Tan","year":"2020","journal-title":"Cell Transplant"},{"key":"10.1016\/j.amjoto.2020.102681_bb0100","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s12935-019-0772-7","article-title":"Pepsin promotes IL-8 signaling-induced epithelial\u2013mesenchymal transition in laryngeal carcinoma","volume":"19","author":"Tan","year":"2019","journal-title":"Cancer Cell Int"},{"key":"10.1016\/j.amjoto.2020.102681_bb0105","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/978-1-62703-529-3_17","article-title":"The comet assay: assessment of in vitro and in vivo DNA damage","volume":"1044","author":"Bajpayee","year":"2013","journal-title":"Methods Mol Biol"},{"issue":"6","key":"10.1016\/j.amjoto.2020.102681_bb0110","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1097\/MLG.0b013e31804154c3","article-title":"Activity\/stability of human pepsin: implications for reflux attributed laryngeal disease","volume":"117","author":"Johnston","year":"2007","journal-title":"Laryngoscope"},{"key":"10.1016\/j.amjoto.2020.102681_bb0115","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s00405-008-0770-1","article-title":"Pathophysiological mechanisms of extraesophageal reflux in otolaryngeal disorders","volume":"266","author":"Schreiber","year":"2009","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"10.1016\/j.amjoto.2020.102681_bb0120","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1177\/000348940911800913","article-title":"Pepsin in nonacidic refluxate can damage hypopharyngeal epithelial cells","volume":"118","author":"Johnston","year":"2009","journal-title":"Ann Otol Rhinol Laryngol"},{"key":"10.1016\/j.amjoto.2020.102681_bb0125","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jvoice.2017.02.011","article-title":"Clinical significance of contralateral reactive lesion in vocal fold polyp and cyst","volume":"32","author":"Cho","year":"2018","journal-title":"J Voice"},{"key":"10.1016\/j.amjoto.2020.102681_bb0130","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jvoice.2009.05.002","article-title":"Vocal polyps: clinical, morphological, and immunohistochemical aspects","volume":"25","author":"Martins","year":"2011","journal-title":"J Voice"},{"key":"10.1016\/j.amjoto.2020.102681_bb0135","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/S1808-8694(15)30596-6","article-title":"Incidence of structural vocal fold abnormalities associated with vocal fold polyps","volume":"74","author":"Eckley","year":"2008","journal-title":"Braz J Otorhinolaryngol"},{"key":"10.1016\/j.amjoto.2020.102681_bb0140","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1080\/00016480410017279","article-title":"Extracellular matrix of Reinke\u2019s space in some pathological conditions","volume":"124","author":"Voli\u0107","year":"2004","journal-title":"Acta Otolaryngol"},{"key":"10.1016\/j.amjoto.2020.102681_bb0145","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1097\/01.moo.0000122306.42961.44","article-title":"Aerodynamics, voice quality, and laryngeal image analysis of normal and pathologic voices","volume":"12","author":"Yumoto","year":"2004","journal-title":"Curr Opin Otolaryngol Head Neck Surg"},{"key":"10.1016\/j.amjoto.2020.102681_bb0150","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1016\/j.cell.2008.03.039","article-title":"Oncogene-induced senescence relayed by an interleukin-dependent inflammatory network","volume":"133","author":"Kuilman","year":"2008","journal-title":"Cell"},{"key":"10.1016\/j.amjoto.2020.102681_bb0155","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.molmed.2010.03.003","article-title":"Inflammatory networks during cellular senescence: causes and consequences","volume":"16","author":"Freund","year":"2010","journal-title":"Trends Mol Med"},{"key":"10.1016\/j.amjoto.2020.102681_bb0160","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1038\/ncb1909","article-title":"Persistent DNA damage signalling triggers senescence-associated inflammatory cytokine secretion","volume":"11","author":"Rodier","year":"2009","journal-title":"Nat Cell Biol"},{"key":"10.1016\/j.amjoto.2020.102681_bb0165","first-page":"120","article-title":"8-Hydroxy-2\u2032 -deoxyguanosine (8-OHdG): a critical biomarker of oxidative stress and carcinogenesis","volume":"27","author":"Valavanidis","year":"2009","journal-title":"J Environ Sci Health C Environ Carcinog Ecotoxicol Rev"},{"key":"10.1016\/j.amjoto.2020.102681_bb0170","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.canlet.2005.07.016","article-title":"Does gammaH2AX foci formation depend on the presence of DNA double strand breaks?","volume":"229","author":"Takahashi","year":"2005","journal-title":"Cancer Lett"},{"issue":"11","key":"10.1016\/j.amjoto.2020.102681_bb0175","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1007\/s11883-014-0452-y","article-title":"8-Hydroxy-2\u2032-deoxyguanosine and cardiovascular disease: a systematic review","volume":"16","author":"Kroese","year":"2014","journal-title":"Curr Atheroscler Rep"},{"issue":"8","key":"10.1016\/j.amjoto.2020.102681_bb0180","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1111\/resp.12378","article-title":"Feasibility of 8-OHdG formation and hOGG1 induction in PBMCs for assessing oxidative DNA damage in the lung of COPD patients","volume":"19","author":"Yang","year":"2014","journal-title":"Respirology"},{"issue":"10","key":"10.1016\/j.amjoto.2020.102681_bb0185","doi-asserted-by":"crossref","first-page":"G863","DOI":"10.1152\/ajpgi.00321.2013","article-title":"Role of intracellular calcium and NADPH oxidase NOX5-S in acid-induced DNA damage in Barrett\u2019s cells and Barrett\u2019s esophageal adenocarcinoma cell","volume":"306","author":"Li","year":"2014","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"issue":"12","key":"10.1016\/j.amjoto.2020.102681_bb0190","first-page":"1161","article-title":"Prevention of DNA damage in Barrett\u2019s esophageal cells exposed to acidic bile salts","volume":"37","author":"Bhardwaj","year":"2016","journal-title":"Carcinogenesis"},{"key":"10.1016\/j.amjoto.2020.102681_bb0195","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.coi.2017.11.002","article-title":"DNA damage signaling and polyploid macrophages in chronic inflammation","volume":"50","author":"Horn","year":"2018","journal-title":"Curr Opin Immunol"},{"issue":"3","key":"10.1016\/j.amjoto.2020.102681_bb0200","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.cmet.2013.08.011","article-title":"DNA damage triggers a chronic autoinflammatory response, leading to fat depletion in NER progeria","volume":"18","author":"Karakasilioti","year":"2013","journal-title":"Cell Metab"},{"key":"10.1016\/j.amjoto.2020.102681_bb0205","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1177\/0963689719838478","article-title":"Optimized generation of primary human epithelial cells from larynx and hypopharynx: a site-specific epithelial model for reflux research","volume":"28","author":"Mo","year":"2019","journal-title":"Cell Transplant"}],"container-title":["American Journal of Otolaryngology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0196070920303756?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0196070920303756?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T05:07:54Z","timestamp":1759295274000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0196070920303756"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":41,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["S0196070920303756"],"URL":"https:\/\/doi.org\/10.1016\/j.amjoto.2020.102681","relation":{},"ISSN":["0196-0709"],"issn-type":[{"value":"0196-0709","type":"print"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Association of pepsin and DNA damage in laryngopharyngeal reflux-related vocal fold polyps","name":"articletitle","label":"Article Title"},{"value":"American Journal of Otolaryngology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.amjoto.2020.102681","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"102681"}}