{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T22:45:04Z","timestamp":1772923504488,"version":"3.50.1"},"reference-count":24,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004263","name":"FAPERGS","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Anaerobe"],"published-print":{"date-parts":[[2016,8]]},"DOI":"10.1016\/j.anaerobe.2016.05.012","type":"journal-article","created":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T19:52:27Z","timestamp":1464205947000},"page":"58-62","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":32,"special_numbering":"C","title":["Protective potential of recombinant non-purified botulinum neurotoxin serotypes C and D"],"prefix":"10.1016","volume":"40","author":[{"suffix":"Jr.","given":"Cl\u00f3vis","family":"Moreira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9199-0764","authenticated-orcid":false,"given":"Carlos Eduardo Pouey","family":"da Cunha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo Mar\u00e7al Schmidt Garcia","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo","family":"Mendon\u00e7a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe Masiero","family":"Salvarani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c2ngela Nunes","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabricio Rochedo","family":"Concei\u00e7\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"Suppl 4","key":"10.1016\/j.anaerobe.2016.05.012_bib1","doi-asserted-by":"crossref","first-page":"D33","DOI":"10.1016\/j.vaccine.2009.08.059","article-title":"Botulism and vaccines for its prevention","volume":"27","author":"Smith","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.anaerobe.2016.05.012_bib2","doi-asserted-by":"crossref","first-page":"4467","DOI":"10.1111\/j.1742-4658.2011.08183.x","article-title":"Molecular structures and functional relationships in clostridial neurotoxins","volume":"278","author":"Swaminathan","year":"2011","journal-title":"FEBS J."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib3","first-page":"1","article-title":"Molecular characterization of a novel botulinum neurotoxin type H gene","author":"Dover","year":"2014","journal-title":"J.\u00a0Infect. Dis. Adv."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib4","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.toxicon.2012.11.011","article-title":"Botulinum neurotoxins: mechanism of action","volume":"67","author":"Tighe","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.anaerobe.2016.05.012_bib5","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/S0968-0004(02)02177-1","article-title":"Botulinum and tetanus neurotoxins: structure, function and therapeutic utility","volume":"27","author":"Turton","year":"2002","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib6","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1615\/CritRevImmunol.v30.i2.50","article-title":"Mode of action of botulinum neurotoxins: current vaccination strategies and molecular immune recognition","volume":"30","author":"Aoki","year":"2010","journal-title":"Crit. Rev. Immunol."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib7","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1128\/IAI.01025-07","article-title":"Subunit vaccine against the seven serotypes of botulism","volume":"76","author":"Baldwin","year":"2008","journal-title":"Infect. Immun."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib8","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1590\/S0100-736X2001000200002","article-title":"Surtos de botulismo em bovinos no Brasil associados \u00e0 ingest\u00e3o de \u00e1gua contaminada","volume":"21","author":"Dutra","year":"2001","journal-title":"Pesq. Vet. Bras."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib9","first-page":"2068","article-title":"Botulism in dairy cattle in Southern Minas Gerais, Brazil","volume":"38","author":"Da Costa","year":"2008","journal-title":"Ci\u00eancia Rural."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib10","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1590\/S0103-84782008000400046","article-title":"Botulismo em ruminantes causado pela ingest\u00e3o de cama-de-frango","volume":"38","author":"Lobato","year":"2008","journal-title":"Ci\u00eancia Rural."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib11","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1590\/S0100-736X2005000200009","article-title":"Botulismo em bovinos de corte e leite alimentados com cama de frango","volume":"25","author":"Dutra","year":"2005","journal-title":"Pesqui. Veterin\u00e1ria Bras."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib12","first-page":"962","article-title":"Ocorr\u00eancia De Botulismo Em Bovinos Confinados No Rio Grande Do Sul","volume":"11","author":"Maboni","year":"2010","journal-title":"Ci\u00eancia Anim. Bras."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib13","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1128\/CDLI.11.3.496-502.2004","article-title":"Vaccination with recombinant whole heavy chain fragments of Clostridium botulinum type C and D Neurotoxins","volume":"11","author":"Arimitsu","year":"2004","journal-title":"Clin. Diagn Lab. Immunol."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib14","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/S0300-9084(00)01173-1","article-title":"Development of vaccines for prevention of botulism","volume":"82","author":"Byrne","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.anaerobe.2016.05.012_bib15","first-page":"701","article-title":"Preparation and Assay of the international standards for Clostridium botulinum types A, B, C, D and E antitoxins","volume":"29","author":"Bowmer","year":"1963","journal-title":"Bull. Wld Hlth Org."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib16","doi-asserted-by":"crossref","first-page":"e69692","DOI":"10.1371\/journal.pone.0069692","article-title":"Production and Evaluation of a recombinant chimeric vaccine against clostridium botulinum neurotoxin types C and D","volume":"8","author":"Gil","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.anaerobe.2016.05.012_bib17","series-title":"Molecular Cloning: a Laboratory Manual","author":"Green","year":"2014"},{"key":"10.1016\/j.anaerobe.2016.05.012_bib18","series-title":"Minist\u00e9rio da Agricultura","author":"Brasil","year":"2002"},{"key":"10.1016\/j.anaerobe.2016.05.012_bib19","doi-asserted-by":"crossref","first-page":"7636","DOI":"10.1016\/j.vaccine.2007.08.051","article-title":"Cattle immune response to botulinum type D toxoid: results of a vaccination study","volume":"25","author":"Steinman","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.anaerobe.2016.05.012_bib20","first-page":"35","article-title":"Pot\u00eancia de tox\u00f3ides botul\u00ednicos bivalentes tipos C e D produzidos e comercializados no Brasil","volume":"20","author":"Lobato","year":"1998","journal-title":"Rev. Bras. Med. Veterin\u00e1ria"},{"key":"10.1016\/j.anaerobe.2016.05.012_bib21","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.mib.2012.05.009","article-title":"Vaccines against botulism","volume":"15","author":"Karalewitz","year":"2012","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib22","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s00449-011-0579-y","article-title":"High level expression of recombinant BoNT\/A-Hc by high cell density cultivation of Escherichia coli","volume":"35","author":"Yari","year":"2012","journal-title":"Bioprocess Biosyst. Eng."},{"key":"10.1016\/j.anaerobe.2016.05.012_bib23","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.vaccine.2013.11.025","article-title":"Vaccination of cattle with a recombinant bivalent toxoid against botulism serotypes C and D","volume":"32","author":"Cunha","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.anaerobe.2016.05.012_bib24","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.tvjl.2007.11.009","article-title":"Veterinary vaccine development from an industrial perspective","volume":"178","author":"Heldens","year":"2008","journal-title":"Vet. J."}],"container-title":["Anaerobe"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1075996416300658?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1075996416300658?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T04:30:48Z","timestamp":1600662648000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1075996416300658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8]]},"references-count":24,"alternative-id":["S1075996416300658"],"URL":"https:\/\/doi.org\/10.1016\/j.anaerobe.2016.05.012","relation":{},"ISSN":["1075-9964"],"issn-type":[{"value":"1075-9964","type":"print"}],"subject":[],"published":{"date-parts":[[2016,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protective potential of recombinant non-purified botulinum neurotoxin serotypes C and D","name":"articletitle","label":"Article Title"},{"value":"Anaerobe","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.anaerobe.2016.05.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}