{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T17:30:34Z","timestamp":1719941434046},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["CBB - APQ-01009-11"],"award-info":[{"award-number":["CBB - APQ-01009-11"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","doi-asserted-by":"publisher","award":["23038000825\/2011-63"],"award-info":[{"award-number":["23038000825\/2011-63"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Phoneutria Biotecnologia e Servi\u00e7os"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Anaerobe"],"published-print":{"date-parts":[[2018,2]]},"DOI":"10.1016\/j.anaerobe.2017.12.004","type":"journal-article","created":{"date-parts":[[2017,12,12]],"date-time":"2017-12-12T18:59:54Z","timestamp":1513105194000},"page":"48-52","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Immunization with a nontoxic naturally occurring Clostridium perfringens alpha toxin induces neutralizing antibodies in rabbits"],"prefix":"10.1016","volume":"49","author":[{"given":"Fl\u00e1via de Faria","family":"Siqueira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo Ot\u00e1vio Silveira","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson Oliveira","family":"do Carmo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara Bruna Ribeiro","family":"de Oliveira-Mendes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Campolina Rebello","family":"Horta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Carlos Faria","family":"Lobato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evanguedes","family":"Kalapothakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.anaerobe.2017.12.004_bib1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.2217\/fmb.13.168","article-title":"Towards an understanding of the role of Clostridium perfringens toxins in human and animal disease","volume":"9","author":"Uzal","year":"2014","journal-title":"Future Microbiol."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib2","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.2217\/fmb.09.72","article-title":"Clostridial toxins","volume":"4","author":"Bouvet","year":"2009","journal-title":"Future Microbiol."},{"issue":"3","key":"10.1016\/j.anaerobe.2017.12.004_bib3","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/S0966-842X(98)01430-9","article-title":"Clostridium perfringens: toxinotype and genotype","volume":"7","author":"Petit","year":"1999","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib4","first-page":"44","article-title":"The synergistic necrohemorrhagic action of Clostridium perfringens perfringolysin and alpha toxin in the bovine intestine and against bovine endothelial cells","volume":"19","author":"Verherstraeten","year":"2013","journal-title":"Vet. Res."},{"issue":"1","key":"10.1016\/j.anaerobe.2017.12.004_bib5","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s13567-016-0336-y","article-title":"The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis","volume":"47","author":"Goossens","year":"2016","journal-title":"Vet. Res."},{"issue":"12","key":"10.1016\/j.anaerobe.2017.12.004_bib6","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1016\/0264-410X(93)90051-X","article-title":"A\u00a0genetically engineered vaccine against the alpha-toxin of Clostridium perfringens protects mice against experimental gas gangrene","volume":"11","author":"Williamson","year":"1993","journal-title":"Vaccine"},{"key":"10.1016\/j.anaerobe.2017.12.004_bib7","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1086\/422691","article-title":"Immunization with the C-domain of \u03b1-toxin prevents lethal infection, localizes tissue injury, and promotes host response to challenge with Clostridium perfringens","volume":"190","author":"Stevens","year":"2004","journal-title":"J.\u00a0Infect. Dis."},{"issue":"7","key":"10.1016\/j.anaerobe.2017.12.004_bib8","doi-asserted-by":"crossref","first-page":"2440","DOI":"10.1128\/IAI.64.7.2440-2444.1996","article-title":"Use of site-directed mutagenesis to probe structure-function relationships of alpha-toxin from Clostridium perfringens","volume":"64","author":"Guillouard","year":"1996","journal-title":"Infect. Immun."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib9","doi-asserted-by":"crossref","first-page":"3489","DOI":"10.1128\/IAI.65.8.3489-3492.1997","article-title":"Site-specific mutagenesis of Clostridium perfringens alpha-toxin: replacement of Asp-56, Asp-130, or Glu-152 causes loss of enzymatic and hemolytic activities","volume":"65","author":"Nagahama","year":"1997","journal-title":"Infect. Immun."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib10","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0022-2836(02)00290-5","article-title":"Crystal structure of the C. perfringens alpha-toxin with the active site closed by a flexible loop region","volume":"319","author":"Eaton","year":"2002","journal-title":"J.\u00a0Mol. Biol."},{"issue":"11","key":"10.1016\/j.anaerobe.2017.12.004_bib11","doi-asserted-by":"crossref","first-page":"7194","DOI":"10.1128\/IAI.69.11.7194-7196.2001","article-title":"Naturally occurring Clostridium perfringens nontoxic alpha-toxin variant as a potential vaccine candidate against alpha-toxin-associated diseases","volume":"69","author":"Schoepe","year":"2001","journal-title":"Infect. Immun."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib12","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.anaerobe.2005.06.003","article-title":"Immunization with an alphatoxin variant 121A\/91-R212H protects mice against Clostridium perfringens alphatoxin","volume":"12","author":"Schoepe","year":"2006","journal-title":"Anaerobe"},{"issue":"2","key":"10.1016\/j.anaerobe.2017.12.004_bib13","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1590\/S0103-84782004000200016","article-title":"Efic\u00e1cia de vacinas comerciais contra clostridioses frente ao desafio com Clostridium sordellii","volume":"34","author":"Lobato","year":"2004","journal-title":"Ci\u00eancia Rural."},{"issue":"1","key":"10.1016\/j.anaerobe.2017.12.004_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S0102-09352004000100001","article-title":"Avalia\u00e7\u00e3o de vacinas contra Clostridium novyi tipo B","volume":"56","author":"Nascimento","year":"2004","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"issue":"2\/3","key":"10.1016\/j.anaerobe.2017.12.004_bib15","first-page":"74","article-title":"Avalia\u00e7\u00e3o da pot\u00eancia de vacinas contra Clostridium tetani comercializadas no Brasil","volume":"10","author":"Lobato","year":"2007","journal-title":"Cienc Vet Trop"},{"issue":"38","key":"10.1016\/j.anaerobe.2017.12.004_bib16","doi-asserted-by":"crossref","first-page":"6125","DOI":"10.1016\/j.vaccine.2010.07.046","article-title":"Potency against enterotoxemia of a recombinant Clostridium perfringens type D epsilon toxoid in ruminants","volume":"28","author":"Lobato","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.anaerobe.2017.12.004_bib17","doi-asserted-by":"crossref","first-page":"5459","DOI":"10.1016\/j.vaccine.2011.05.059","article-title":"Potential protective immunogenicity of recombinant Clostridium perfringens \u03b1-\u03b22-\u03b21 fusion toxin in mice, sows and cows","volume":"29","author":"Zeng","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.anaerobe.2017.12.004_bib18","doi-asserted-by":"crossref","first-page":"4152","DOI":"10.1016\/j.vaccine.2013.06.094","article-title":"Vaccination with recombinant Clostridium perfringens toxoids \u03b1 and \u03b2 promotes elevated antepartum and passive humoral immunity in swine","volume":"31","author":"Salvarani","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.anaerobe.2017.12.004_bib19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep22816","article-title":"Immunogenicity of a trivalent recombinant vaccine against Clostridium perfringens alpha, beta, and epsilon toxins in Farm ruminants","volume":"6","author":"Moreira","year":"2016","journal-title":"Sci. Rep."},{"issue":"3\u20134","key":"10.1016\/j.anaerobe.2017.12.004_bib20","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.vetmic.2012.04.012","article-title":"Characterization of polymorphisms and isoforms of the Clostridium perfringens phospholipase C gene (plc) reveals high genetic diversity","volume":"159","author":"Siqueira","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib21","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.anaerobe.2017.12.004_bib22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002693","article-title":"Molecular, immunological, and biological characterization of tityus serrulatus venom hyaluronidase: New insights into its role in envenomation","volume":"8","author":"Horta","year":"2014","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib23","first-page":"02","article-title":"Conditional licenses for products containing Clostridium perfringens type A","author":"United States Department of Agriculture (USDA)","year":"2002","journal-title":"Cent. Vet. Biol. Not."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib24","first-page":"493","article-title":"A\u00a0simple method of estimating fifty per cent end points","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Hyg."},{"issue":"8","key":"10.1016\/j.anaerobe.2017.12.004_bib25","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1038\/1447","article-title":"Structure of the key toxin in gas gangrene","volume":"5","author":"Naylor","year":"1998","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib26","doi-asserted-by":"crossref","first-page":"6253","DOI":"10.1021\/bi012015v","article-title":"The first strain of Clostridium perfringens isolated from an avian source has an alpha-toxin with divergent structural and kinetic properties","volume":"41","author":"Justin","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.anaerobe.2017.12.004_bib27","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1081\/TXR-200038417","article-title":"The bacterial zinc-metallophospholipases, C","volume":"23","author":"Titball","year":"2004","journal-title":"J.\u00a0Toxicol. - Toxin Rev."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib28","doi-asserted-by":"crossref","first-page":"D44","DOI":"10.1016\/j.vaccine.2009.07.047","article-title":"Clostridium perfringens vaccines","volume":"27","author":"Titball","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.anaerobe.2017.12.004_bib29","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.vetmic.2009.07.003","article-title":"Clostridia as agents of zoonotic disease","volume":"140","author":"Songer","year":"2010","journal-title":"Vet. Microbiol."},{"issue":"1","key":"10.1016\/j.anaerobe.2017.12.004_bib30","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1128\/CMR.3.1.66","article-title":"Toxigenic clostridia","volume":"3","author":"Hatheway","year":"1990","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib31","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.vetmic.2004.03.002","article-title":"Efficacy of vaccines against bacterial diseases in swine: what can we expect","volume":"100","author":"Haesebrouck","year":"2004","journal-title":"Vet. Microbiol."},{"issue":"5","key":"10.1016\/j.anaerobe.2017.12.004_bib32","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1111\/j.1348-0421.1979.tb00468.x","article-title":"Effect of carbohydrates and control of culture pH on beta toxin production by C. perfringens type C","volume":"23","author":"Sakurai","year":"1979","journal-title":"Microbiol. Immunol."},{"issue":"4","key":"10.1016\/j.anaerobe.2017.12.004_bib33","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1128\/MMBR.55.4.621-648.1991","article-title":"Molecular genetics and pathogenesis of Clostridium perfringens","volume":"55","author":"Rood","year":"1991","journal-title":"Microbiol. Rev."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib34","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1007\/s00203-009-0510-y","article-title":"Selection of a Clostridium perfringens type D epsilon toxin producer via dot-blot test","volume":"191","author":"Gon\u00e7alves","year":"2009","journal-title":"Arch. Microbiol."},{"issue":"11","key":"10.1016\/j.anaerobe.2017.12.004_bib35","doi-asserted-by":"crossref","first-page":"5257","DOI":"10.1128\/IAI.00686-08","article-title":"Recombinant Bacillus subtilis expressing the Clostridium perfringens alpha toxoid is a candidate orally delivered vaccine against Necrotic Enteritis","volume":"76","author":"Hoang","year":"2008","journal-title":"Infect. Immun."},{"issue":"5","key":"10.1016\/j.anaerobe.2017.12.004_bib36","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1128\/CVI.00457-07","article-title":"Recombinant attenuated Salmonella enterica serovar Typhimurium expressing the carboxy-terminal domain of alpha toxin from Clostridium perfringens induces protective responses against necrotic enteritis in chickens","volume":"15","author":"Zekarias","year":"2008","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib37","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.vetmic.2008.06.001","article-title":"Immunization with recombinant alpha toxin partially protects broiler chicks against experimental challenge with Clostridium perfringens","volume":"133","author":"Cooper","year":"2009","journal-title":"Vet. Microbiol."},{"issue":"2","key":"10.1016\/j.anaerobe.2017.12.004_bib38","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1128\/CVI.00406-09","article-title":"A\u00a0live oral recombinant Salmonella enterica Serovar Typhimurium vaccine expressing Clostridium perfringens antigens confers protection against Necrotic Enteritis in broiler chickens","volume":"17","author":"Kulkarni","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib39","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.vetmic.2011.11.019","article-title":"Evaluation in broilers of the probiotic properties of Pichia pastoris and a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene","volume":"156","author":"De los Santos","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.anaerobe.2017.12.004_bib40","doi-asserted-by":"crossref","first-page":"5401","DOI":"10.1016\/j.vaccine.2012.06.007","article-title":"Vaccination with Clostridium perfringens recombinant proteins in combination with MontanideTM ISA 71 VG adjuvant increases protection against experimental necrotic enteritis in commercial broiler chickens","volume":"30","author":"Jang","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.anaerobe.2017.12.004_bib41","doi-asserted-by":"crossref","first-page":"D33","DOI":"10.1016\/j.vaccine.2009.08.059","article-title":"Botulism and vaccines for its prevention","volume":"27","author":"Smith","year":"2009","journal-title":"Vaccine"}],"container-title":["Anaerobe"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1075996417302275?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1075996417302275?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,24]],"date-time":"2020-10-24T02:07:54Z","timestamp":1603505274000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1075996417302275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":41,"alternative-id":["S1075996417302275"],"URL":"https:\/\/doi.org\/10.1016\/j.anaerobe.2017.12.004","relation":{},"ISSN":["1075-9964"],"issn-type":[{"value":"1075-9964","type":"print"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Immunization with a nontoxic naturally occurring Clostridium perfringens alpha toxin induces neutralizing antibodies in rabbits","name":"articletitle","label":"Article Title"},{"value":"Anaerobe","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.anaerobe.2017.12.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}