{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:10:16Z","timestamp":1791378616345,"version":"4.3.2"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.es","elsevier.es","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Revista Internacional de Androlog\u00eda"],"published-print":{"date-parts":[[2022,10]]},"DOI":"10.1016\/j.androl.2021.02.003","type":"journal-article","created":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T06:29:50Z","timestamp":1657261790000},"page":"S24-S30","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"S1","title":["Cyclic adenosine monophosphate (cAMP) analog and phosphodiesterase inhibitor (IBMX) ameliorate human sperm capacitation and motility"],"prefix":"10.1016","volume":"20","author":[{"given":"Hamid","family":"Piroozmanesh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahil","family":"Jannatifar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyyed Meisam","family":"Ebrahimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoda","family":"Fazaeli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lila","family":"Naserpoor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Dimitriadis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Nejatbakhsh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.androl.2021.02.003_bib0180","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/j.1365-2605.2005.00630.x","article-title":"Sperm function tests and fertility","volume":"29","author":"Aitken","year":"2006","journal-title":"Int J Androl"},{"key":"10.1016\/j.androl.2021.02.003_bib0185","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/aja.2012.81","article-title":"Mechanism of sperm capacitation and the acrosome reaction: role of protein kinases","volume":"14","author":"Ickowicz","year":"2012","journal-title":"Asian J Androl"},{"issue":"Suppl. 1","key":"10.1016\/j.androl.2021.02.003_bib0190","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/j.1439-0272.2010.01150.x","article-title":"Effect of procaine, pentoxifylline and trolox on capacitation and hyperactivation of stallion spermatozoa","volume":"44","author":"Ortgies","year":"2012","journal-title":"Andrologia"},{"key":"10.1016\/j.androl.2021.02.003_bib0195","series-title":"Spermatogenesis","first-page":"113","article-title":"Methods for the assessment of sperm capacitation and acrosome reaction excluding the sperm penetration assay","author":"De Jonge","year":"2013"},{"key":"10.1016\/j.androl.2021.02.003_bib0200","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1093\/molehr\/8.9.811","article-title":"Different signal transduction pathways are involved during human sperm capacitation induced by biological and pharmacological agents","volume":"8","author":"Thundathil","year":"2002","journal-title":"Mol Hum Reprod"},{"key":"10.1016\/j.androl.2021.02.003_bib0205","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1530\/rep.0.1260183","article-title":"Involvement of tyrosine kinase and cAMP-dependent kinase cross-talk in the regulation of human sperm motility","volume":"126","author":"Bajpai","year":"2003","journal-title":"Reproduction"},{"key":"10.1016\/j.androl.2021.02.003_bib0210","first-page":"99","article-title":"Impaired hyperactivation of human sperm in patients with infertility","volume":"51","author":"Munire","year":"2004","journal-title":"J Med Dent Sci"},{"key":"10.1016\/j.androl.2021.02.003_bib0215","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1111\/and.12068","article-title":"Assessment of sperm hyperactivated motility and acrosome reaction can discriminate the use of spermatozoa for conventional in vitro fertilisation or intracytoplasmic sperm injection: preliminary results","volume":"46","author":"Wiser","year":"2014","journal-title":"Andrologia"},{"key":"10.1016\/j.androl.2021.02.003_bib0220","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1055\/s-2007-979867","article-title":"protein (leptin) pathway-a link between adipose tissue mass and central neural networks","volume":"28","author":"Campfield","year":"1996","journal-title":"Horm Metab Res"},{"key":"10.1016\/j.androl.2021.02.003_bib0225","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1093\/molehr\/gax030","article-title":"Extracellular cAMP activates molecular signalling pathways associated with sperm capacitation in bovines","volume":"23","author":"Alonso","year":"2017","journal-title":"Mol Hum Reprod"},{"key":"10.1016\/j.androl.2021.02.003_bib0230","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1111\/j.1439-0272.1989.tb02443.x","article-title":"Correlation between the sperm motility and the adenylate cyclase activity in infertile men","volume":"21","author":"Ishikawa","year":"1989","journal-title":"Andrologia"},{"key":"10.1016\/j.androl.2021.02.003_bib0235","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1111\/j.1439-0272.1985.tb01728.x","article-title":"Further studies on the effects of pentoxifylline on sperm count and sperm motility in patients with idiopathic oligo-asthenozoospermia","volume":"17","author":"Marrama","year":"1985","journal-title":"Andrologia"},{"key":"10.1016\/j.androl.2021.02.003_bib0240","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1093\/molehr\/3.3.175","article-title":"Capacitation as a regulatory event that primes spermatozoa for the acrosome reaction and fertilization","volume":"3","author":"de Lamirande","year":"1997","journal-title":"Mol Hum Reprod"},{"key":"10.1016\/j.androl.2021.02.003_bib0245","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.anireprosci.2016.07.001","article-title":"Calcium regulates motility and protein phosphorylation by changing cAMP and ATP concentrations in boar sperm in vitro","volume":"172","author":"Li","year":"2016","journal-title":"Anim Reprod Sci"},{"key":"10.1016\/j.androl.2021.02.003_bib0250","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1095\/biolreprod56.3.707","article-title":"Regulation of protein tyrosine phosphorylation during bovine sperm capacitation by a cyclic adenosine 3\u2032,5\u2032-monophosphate-dependent pathway","volume":"56","author":"Galantino-Homer","year":"1997","journal-title":"Biol Reprod"},{"key":"10.1016\/j.androl.2021.02.003_bib0255","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1002\/mrd.20034","article-title":"Bovine sperm capacitation: assessment of phosphodiesterase activity and intracellular alkalinization on capacitation-associated protein tyrosine phosphorylation","volume":"67","author":"Galantino-Homer","year":"2004","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.androl.2021.02.003_bib0260","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1093\/humrep\/13.5.1248","article-title":"Enhancement of motility and acrosome reaction in human spermatozoa: differential activation by type-specific phosphodiesterase inhibitors","volume":"13","author":"Fisch","year":"1998","journal-title":"Hum Reprod"},{"key":"10.1016\/j.androl.2021.02.003_bib0265","series-title":"WHO laboratory manual for the examination and processing of human semen","author":"WH Organization","year":"2010"},{"key":"10.1016\/j.androl.2021.02.003_bib0270","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1095\/biolreprod51.6.1099","article-title":"Differences in the role of cyclic adenosine 3\u2032,5\u2032-monophosphate during capacitation of bovine sperm by heparin or oviduct fluid","volume":"51","author":"Parrish","year":"1994","journal-title":"Biol Reprod"},{"key":"10.1016\/j.androl.2021.02.003_bib0275","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1095\/biolreprod28.1.75","article-title":"Cyclic adenosine 3\u2032,5\u2032 monophosphate, calcium and protein phosphorylation in flagellar motility","volume":"28","author":"Tash","year":"1983","journal-title":"Biol Reprod"},{"key":"10.1016\/j.androl.2021.02.003_bib0280","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1439-0272.1992.tb02609.x","article-title":"Effect of Sp-cAMP on sperm motility in patients with unexplained infertility","volume":"24","author":"Agarwal","year":"1992","journal-title":"Andrologia"},{"key":"10.1016\/j.androl.2021.02.003_bib0285","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1002\/cm.970200407","article-title":"The calcium-induced curvature reversal of rat sperm is potentiated by cAMP and inhibited by anti-calmodulin","volume":"20","author":"Lindemann","year":"1991","journal-title":"Cell Motil Cytoskeleton"},{"key":"10.1016\/j.androl.2021.02.003_bib0290","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1002\/mrd.1120220205","article-title":"Relationship between calcium, cyclic AMP, ATP, and intracellular pH and the capacity of hamster spermatozoa to express hyperactivated motility","volume":"22","author":"White","year":"1989","journal-title":"Gamete Res"},{"key":"10.1016\/j.androl.2021.02.003_bib0295","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M508854200","article-title":"Calcium induced acrosomal exocytosis requires cAMP acting through a PKA-independent, EPAC-mediated pathway","author":"Branham","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1016\/j.androl.2021.02.003_bib0300","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1002\/j.1939-4640.2000.tb03424.x","article-title":"The cyclic GMP-specific phosphodiesterase inhibitor, sildenafil, stimulates human sperm motility and capacitation but not acrosome reaction","volume":"21","author":"Lefievre","year":"2000","journal-title":"J Androl"},{"key":"10.1016\/j.androl.2021.02.003_bib0305","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1093\/humrep\/dev154","article-title":"Fibronectin stimulates human sperm capacitation through the cyclic AMP\/protein kinase A pathway","volume":"30","author":"Martinez-Leon","year":"2015","journal-title":"Hum Reprod"},{"issue":"Pt 19","key":"10.1016\/j.androl.2021.02.003_bib0310","doi-asserted-by":"crossref","first-page":"3543","DOI":"10.1242\/jcs.114.19.3543","article-title":"Bicarbonate stimulated phospholipid scrambling induces cholesterol redistribution and enables cholesterol depletion in the sperm plasma membrane","volume":"114","author":"Flesch","year":"2001","journal-title":"J Cell Sci"},{"key":"10.1016\/j.androl.2021.02.003_bib0315","doi-asserted-by":"crossref","first-page":"7001","DOI":"10.1074\/jbc.M206284200","article-title":"Involvement of a Na+\/HCO cotransporter in mouse sperm capacitation","volume":"278","author":"Demarco","year":"2003","journal-title":"J Biol Chem"},{"key":"10.1016\/j.androl.2021.02.003_bib0320","doi-asserted-by":"crossref","first-page":"18543","DOI":"10.1073\/pnas.1317113110","article-title":"Ca2+ ionophore A23187 can make mouse spermatozoa capable of fertilizing in vitro without activation of cAMP-dependent phosphorylation pathways","volume":"110","author":"Tateno","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.androl.2021.02.003_bib0325","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1242\/dev.121.4.1139","article-title":"Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway","volume":"121","author":"Visconti","year":"1995","journal-title":"Development"},{"key":"10.1016\/j.androl.2021.02.003_bib0330","first-page":"105","article-title":"Progress toward understanding the molecular basis of capacitation","volume":"1","author":"Florman","year":"1991","journal-title":"Elem Mammal Fertil"},{"key":"10.1016\/j.androl.2021.02.003_bib0335","doi-asserted-by":"crossref","first-page":"35307","DOI":"10.1074\/jbc.M113.489476","article-title":"Compartmentalization of distinct cAMP signaling pathways in mammalian sperm","volume":"288","author":"Wertheimer","year":"2013","journal-title":"J Biol Chem"},{"key":"10.1016\/j.androl.2021.02.003_bib0340","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1002\/(SICI)1098-2795(200002)55:2<220::AID-MRD12>3.0.CO;2-I","article-title":"cAMP-dependent protein kinase control of plasma membrane lipid architecture in boar sperm","volume":"55","author":"Harrison","year":"2000","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.androl.2021.02.003_bib0345","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1002\/mrd.20028","article-title":"Rapid PKA-catalysed phosphorylation of boar sperm proteins induced by the capacitating agent bicarbonate","volume":"67","author":"Harrison","year":"2004","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.androl.2021.02.003_bib0350","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1006\/dbio.2002.0728","article-title":"Targeted disruption of the Akap4 gene causes defects in sperm flagellum and motility","volume":"248","author":"Miki","year":"2002","journal-title":"Dev Biol"}],"container-title":["Revista Internacional de Androlog\u00eda"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1698031X2200036X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1698031X2200036X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T05:31:57Z","timestamp":1700803917000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1698031X2200036X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10]]},"references-count":35,"alternative-id":["S1698031X2200036X"],"URL":"https:\/\/doi.org\/10.1016\/j.androl.2021.02.003","relation":{},"ISSN":["1698-031X"],"issn-type":[{"value":"1698-031X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cyclic adenosine monophosphate (cAMP) analog and phosphodiesterase inhibitor (IBMX) ameliorate human sperm capacitation and motility","name":"articletitle","label":"Article Title"},{"value":"Revista Internacional de Androlog\u00eda","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.androl.2021.02.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Asociaci\u00f3n Espa\u00f1ola de Androlog\u00eda, Medicina Sexual y Reproductiva. Published by Elsevier Espa\u00f1a, S.L.U. All rights reserved.","name":"copyright","label":"Copyright"}]}}