{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:25:08Z","timestamp":1790882708382,"version":"4.1.0"},"reference-count":51,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T00:00:00Z","timestamp":1596672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2016YFC0501805"],"award-info":[{"award-number":["2016YFC0501805"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31902184"],"award-info":[{"award-number":["31902184"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2019M653774"],"award-info":[{"award-number":["2019M653774"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Animal"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1016\/j.animal.2020.100019","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T00:56:52Z","timestamp":1613609812000},"page":"100019","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves"],"prefix":"10.1016","volume":"15","author":[{"given":"S.R.","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.D.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.L.","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.T.","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.H.","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.H.","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.animal.2020.100019_bb0005","doi-asserted-by":"crossref","first-page":"666","DOI":"10.3168\/jds.2016-10871","article-title":"Lipopolysaccharide derived from the digestive tract provokes oxidative stress in the liver of dairy cows fed a high-grain diet","volume":"100","author":"Abaker","year":"2017","journal-title":"Journal of Dairy Science"},{"key":"10.1016\/j.animal.2020.100019_bb0010","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1017\/S0962728600030232","article-title":"Indicators of undernutrition in cattle","volume":"15","author":"Agen\u00e4s","year":"2006","journal-title":"Animal Welfare"},{"key":"10.1016\/j.animal.2020.100019_bb4010","series-title":"Bovine endotoxicosis: Aspects of relevance to ruminal acidosis","author":"Andersen","year":"2000"},{"key":"10.1016\/j.animal.2020.100019_bb4005","series-title":"Official Methods of Analysis","author":"AOAC International","year":"2000"},{"key":"10.1016\/j.animal.2020.100019_bb0015","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0099411","article-title":"The intestinal archaea Methanosphaera stadtmanae and Methanobrevibacter smithii activate human dendritic cells","volume":"9","author":"Bang","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.animal.2020.100019_bb0020","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/S0021-9258(18)51379-X","article-title":"The colorimetric determination of lactic acid in biological material","volume":"138","author":"Barker","year":"1941","journal-title":"The Journal of Biological Chemistry"},{"key":"10.1016\/j.animal.2020.100019_bb0025","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s12866-018-1213-9","article-title":"Effects of dietary energy levels on rumen bacterial community composition in Holstein heifers under the same forage to concentrate ratio condition","volume":"18","author":"Bi","year":"2018","journal-title":"BMC Microbiology"},{"key":"10.1016\/j.animal.2020.100019_bb0030","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.animal.2020.100019_bb0035","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1093\/bioinformatics\/btp636","article-title":"PyNAST: a flexible tool for aligning sequences to a template alignment","volume":"26","author":"Caporaso","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.animal.2020.100019_bb0040","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nature Methods"},{"key":"10.1016\/j.animal.2020.100019_bb0045","doi-asserted-by":"crossref","first-page":"4516","DOI":"10.1073\/pnas.1000080107","article-title":"Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample","volume":"108","author":"Caporaso","year":"2011","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"10.1016\/j.animal.2020.100019_bb0050","first-page":"1736","article-title":"Effects of Forage:Concentrate Ratio on Growth Performance, Ruminal Fermentation and Blood Metabolites in Housing-feeding Yaks, Asian-Australasian","volume":"28","author":"Chen","year":"2015","journal-title":"Journal of Animal Science"},{"key":"10.1016\/j.animal.2020.100019_bb0055","doi-asserted-by":"crossref","first-page":"162","DOI":"10.3389\/fmicb.2019.00162","article-title":"Megasphaera elsdenii Lactate Degradation Pattern Shifts in Rumen Acidosis Models","volume":"10","author":"Chen","year":"2019","journal-title":"Frontiers in Microbiology"},{"key":"10.1016\/j.animal.2020.100019_bb0060","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1053\/j.gastro.2017.09.006","article-title":"Intestinal dysbiosis featuring abundance of Ruminococcus gnavus associates with allergic diseases in infants","volume":"154","author":"Chua","year":"2018","journal-title":"Gastroenterology"},{"key":"10.1016\/j.animal.2020.100019_bb0065","doi-asserted-by":"crossref","first-page":"994","DOI":"10.3389\/fmicb.2020.00994","article-title":"Effect of Alfalfa Hay and Starter Feeding Intervention on Gastrointestinal Microbial Community, Growth and Immune Performance of Yak Calves","volume":"11","author":"Cui","year":"2020","journal-title":"Frontiers in Microbiology"},{"key":"10.1016\/j.animal.2020.100019_bb0070","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1017\/S1751731119002106","article-title":"Effects of intravenous arginine infusion on inflammation and metabolic indices of dairy cows in early lactation","volume":"14","author":"Ding","year":"2020","journal-title":"Animal"},{"key":"10.1016\/j.animal.2020.100019_bb0075","doi-asserted-by":"crossref","first-page":"193","DOI":"10.2298\/BAH1702193D","article-title":"Serum enzyme activities in blood and milk in the different stage of lactation in holstein dairy cows","volume":"33","author":"\u0110okovi\u0107","year":"2017","journal-title":"Biotechnology in Animal Husbandry"},{"key":"10.1016\/j.animal.2020.100019_bb0080","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: highly accurate OTU sequences from microbial amplicon reads","volume":"10","author":"Edgar","year":"2013","journal-title":"Nature Methods"},{"key":"10.1016\/j.animal.2020.100019_bb0085","article-title":"SINTAX: a simple non-Bayesian taxonomy classifier for 16S and ITS sequences","volume":"074161","author":"Edgar","year":"2016","journal-title":"bioRxiv"},{"key":"10.1016\/j.animal.2020.100019_bb0090","doi-asserted-by":"crossref","first-page":"3476","DOI":"10.1093\/bioinformatics\/btv401","article-title":"Error filtering, pair assembly and error correction for next-generation sequencing reads","volume":"31","author":"Edgar","year":"2015","journal-title":"Bioinformatics"},{"key":"10.1016\/j.animal.2020.100019_bb0095","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1071\/BI9680177","article-title":"Volatile fatty acids in the caecum of the sheep","volume":"21","author":"Faichney","year":"1968","journal-title":"Australian Journal of Biological Sciences"},{"key":"10.1016\/j.animal.2020.100019_bb0100","doi-asserted-by":"crossref","first-page":"289","DOI":"10.4161\/gmic.19897","article-title":"Microbial degradation of complex carbohydrates in the gut","volume":"3","author":"Flint","year":"2012","journal-title":"Gut Microbes"},{"key":"10.1016\/j.animal.2020.100019_bb0105","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.2527\/1999.7751120x","article-title":"Interaction of cattle health\/immunity and nutrition","volume":"77","author":"Galyean","year":"1999","journal-title":"Journal of Animal Science"},{"key":"10.1016\/j.animal.2020.100019_bb0110","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/jnc.14333","article-title":"Antibiotics-induced modulation of large intestinal microbiota altered aromatic amino acid profile and expression of neurotransmitters in the hypothalamus of piglets","volume":"146","author":"Gao","year":"2018","journal-title":"Journal of Neurochemistry"},{"key":"10.1016\/j.animal.2020.100019_bb0115","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1007\/s11938-019-00241-6","article-title":"Interplay of liver disease and gut microbiota in the development of colorectal neoplasia","volume":"17","author":"Gleeson","year":"2019","journal-title":"Current Treatment Options in Gastroenterology"},{"key":"10.1016\/j.animal.2020.100019_bb0120","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.3168\/jds.S0022-0302(05)72807-1","article-title":"Subacute ruminal acidosis induces ruminal lipopolysaccharide endotoxin release and triggers an inflammatory response","volume":"88","author":"Gozho","year":"2005","journal-title":"Journal of Dairy Science"},{"key":"10.1016\/j.animal.2020.100019_bb0125","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.2527\/jas.2010-3460","article-title":"Ruminant nutrition symposium: productivity, digestion, and health responses to hindgut acidosis in ruminants","volume":"89","author":"Gressley","year":"2011","journal-title":"Journal of Animal Science"},{"key":"10.1016\/j.animal.2020.100019_bb0130","doi-asserted-by":"crossref","first-page":"46769","DOI":"10.18632\/oncotarget.18151","article-title":"Rumen-derived lipopolysaccharide provoked inflammatory injury in the liver of dairy cows fed a high-concentrate diet","volume":"8","author":"Guo","year":"2017","journal-title":"Oncotarget"},{"key":"10.1016\/j.animal.2020.100019_bb0135","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/mi.2009.132","article-title":"Metagenomic analyses reveal antibiotic-induced temporal and spatial changes in intestinal microbiota with associated alterations in immune cell homeostasis","volume":"3","author":"Hill","year":"2010","journal-title":"Mucosal Immunology"},{"key":"10.1016\/j.animal.2020.100019_bb0140","doi-asserted-by":"crossref","first-page":"945785","DOI":"10.1155\/2010\/945785","article-title":"Methanogens: methane producers of the Rumen and mitigation strategies","volume":"2010","author":"Hook","year":"2010","journal-title":"Archaea"},{"key":"10.1016\/j.animal.2020.100019_bb0145","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1017\/S1751731115002499","article-title":"Determining optimum age of Holstein dairy calves when adding chopped alfalfa hay to meal starter diets based on measures of growth and performance","volume":"10","author":"Hosseini","year":"2016","journal-title":"Animal"},{"key":"10.1016\/j.animal.2020.100019_bb0150","doi-asserted-by":"crossref","first-page":"194","DOI":"10.5958\/0973-9718.2017.00060.5","article-title":"Functional liver stress in dairy sheep","volume":"23","author":"Hrkovic-Porobija","year":"2017","journal-title":"Indian Journal of Small Ruminants"},{"key":"10.1016\/j.animal.2020.100019_bb0155","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1016\/j.bbmt.2015.04.016","article-title":"Intestinal blautia is associated with reduced death from graft-versus-host disease","volume":"21","author":"Jenq","year":"2015","journal-title":"Biology of Blood and Marrow Transplantation"},{"key":"10.1016\/j.animal.2020.100019_bb0160","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1007\/s00535-015-1084-x","article-title":"A breakthrough in probiotics: clostridium butyricum regulates gut homeostasis and anti-inflammatory response in inflammatory bowel disease","volume":"50","author":"Kanai","year":"2015","journal-title":"Journal of Gastroenterology"},{"key":"10.1016\/j.animal.2020.100019_bb0165","doi-asserted-by":"crossref","first-page":"5080","DOI":"10.1128\/IAI.72.9.5080-5088.2004","article-title":"Molecular evolutionary relationships of enteroinvasive Escherichia coli and Shigella spp","volume":"72","author":"Lan","year":"2004","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.animal.2020.100019_bb0170","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1038\/nbt.2676","article-title":"Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences","volume":"31","author":"Langille","year":"2013","journal-title":"Nature Biotechnology"},{"key":"10.1016\/j.animal.2020.100019_bb0175","doi-asserted-by":"crossref","first-page":"45840","DOI":"10.1038\/srep45840","article-title":"Microbial biogeography and core microbiota of the rat digestive tract","volume":"7","author":"Li","year":"2017","journal-title":"Scientific Reports"},{"key":"10.1016\/j.animal.2020.100019_bb0180","first-page":"429","article-title":"Starter feeding supplementation alters colonic mucosal bacterial communities and modulates mucosal immune Homeostasis in Newborn Lambs","volume":"8","author":"Liu","year":"2017","journal-title":"Frontiers in Microbiology"},{"key":"10.1016\/j.animal.2020.100019_bb0185","doi-asserted-by":"crossref","first-page":"421","DOI":"10.3389\/fimmu.2016.00421","article-title":"Increased TNF-\u03b1\/IFN-\u03b3\/IL-2 and decreased TNF-\u03b1\/IFN-\u03b3 production by central memory T cells are associated with protective responses against bovine tuberculosis following BCG vaccination","volume":"7","author":"Maggioli","year":"2016","journal-title":"Frontiers in Immunology"},{"key":"10.1016\/j.animal.2020.100019_bb4000","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1007\/s11250-011-9819-z","article-title":"Rumen development and growth of Balouchi lambs offered alfalfa hay pre-and post-weaning","volume":"43","author":"Norouzian","year":"2011","journal-title":"Trop. Anim. Health Proc."},{"key":"10.1016\/j.animal.2020.100019_bb0190","doi-asserted-by":"crossref","first-page":"313","DOI":"10.3168\/jds.2019-17360","article-title":"Effects of milk replacer feeding levels on performance and metabolism of preweaned dairy calves during summer","volume":"103","author":"Orellana Rivas","year":"2010","journal-title":"Journal of Dairy Science"},{"key":"10.1016\/j.animal.2020.100019_bb0195","doi-asserted-by":"crossref","first-page":"3123","DOI":"10.1093\/bioinformatics\/btu494","article-title":"STAMP: statistical analysis of taxonomic and functional profiles","volume":"30","author":"Parks","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.animal.2020.100019_bb0200","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1038\/nmeth.2658","article-title":"Differential abundance analysis for microbial marker-gene surveys","volume":"10","author":"Paulson","year":"2013","journal-title":"Nature Methods"},{"key":"10.1016\/j.animal.2020.100019_bb0205","doi-asserted-by":"crossref","first-page":"560","DOI":"10.3390\/ani9080560","article-title":"Dynamic variations in fecal bacterial community and fermentation profile of Holstein steers in response to three stepwise density diets","volume":"9","author":"Qiu","year":"2019","journal-title":"Animals"},{"key":"10.1016\/j.animal.2020.100019_bb0210","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1073\/pnas.0915162107","article-title":"Neisseria meningitidis GNA2132, a heparin-binding protein that induces protective immunity in humans","volume":"107","author":"Serruto","year":"2010","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/j.animal.2020.100019_bb0215","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1002\/eji.200526157","article-title":"Cell wall glycosphingolipids of Sphingomonas paucimobilis are CD1d-specific ligands for NKT cells","volume":"35","author":"Sriram","year":"2005","journal-title":"European Journal of Immunology"},{"key":"10.1016\/j.animal.2020.100019_bb0220","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1038\/ng.2906","article-title":"Pathogens and host immunity in the ancient human oral cavity","volume":"46","author":"Warinner","year":"2014","journal-title":"Nature Genetics"},{"key":"10.1016\/j.animal.2020.100019_bb0225","doi-asserted-by":"crossref","first-page":"921","DOI":"10.3390\/ani9110921","article-title":"Changed caecal microbiota and fermentation contribute to the beneficial effects of early weaning with Alfalfa Hay, starter feed, and milk replacer on the growth and organ development of Yak Calves","volume":"9","author":"Wu","year":"2019","journal-title":"Animals"},{"key":"10.1016\/j.animal.2020.100019_bb0230","doi-asserted-by":"crossref","first-page":"828","DOI":"10.3382\/ps\/pey393","article-title":"Effects of glucose oxidase on growth performance, gut function, and cecal microbiota of broiler chickens","volume":"98","author":"Wu","year":"2019","journal-title":"Poultry Science"},{"key":"10.1016\/j.animal.2020.100019_bb0235","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.syapm.2017.03.006","article-title":"Rumen prokaryotic communities of ruminants under different feeding paradigms on the Qinghai-Tibetan Plateau","volume":"40","author":"Xue","year":"2017","journal-title":"Systematic and Applied Microbiology"},{"key":"10.1016\/j.animal.2020.100019_bb0240","doi-asserted-by":"crossref","first-page":"574","DOI":"10.3389\/fmicb.2018.00574","article-title":"Alfalfa intervention alters rumen microbial community development in Hu Lambs during early life","volume":"9","author":"Yang","year":"2018","journal-title":"Frontiers in Microbiology"}],"container-title":["Animal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1751731120300197?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1751731120300197?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T23:44:36Z","timestamp":1761695076000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1751731120300197"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":51,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["S1751731120300197"],"URL":"https:\/\/doi.org\/10.1016\/j.animal.2020.100019","relation":{},"ISSN":["1751-7311"],"issn-type":[{"value":"1751-7311","type":"print"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves","name":"articletitle","label":"Article Title"},{"value":"Animal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.animal.2020.100019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Published by Elsevier B.V. on behalf of The Animal Consortium.","name":"copyright","label":"Copyright"}],"article-number":"100019"}}