{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:28:43Z","timestamp":1785338923333,"version":"3.55.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T00:00:00Z","timestamp":1633392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004829","name":"Sichuan Province Department of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012542","name":"Sichuan Province Science and Technology Support Program","doi-asserted-by":"publisher","award":["2020YJ0398"],"award-info":[{"award-number":["2020YJ0398"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Animal Nutrition"],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.1016\/j.aninu.2021.06.016","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T06:45:20Z","timestamp":1634193920000},"page":"135-143","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Spermine protects intestinal barrier integrity through ras-related C3 botulinum toxin substrate 1\/phospholipase C-\u03b31 signaling pathway in piglets"],"prefix":"10.1016","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2385-7528","authenticated-orcid":false,"given":"Guangmang","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caimei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aninu.2021.06.016_bib1","doi-asserted-by":"crossref","first-page":"C164","DOI":"10.1152\/ajpcell.00247.2010","article-title":"The phosphorylation state of MRLC is polyamine dependent in intestinal epithelial cells","volume":"300","author":"Bavaria","year":"2011","journal-title":"Am J Physiol Cell Physiol"},{"key":"10.1016\/j.aninu.2021.06.016_bib2","doi-asserted-by":"crossref","first-page":"C327","DOI":"10.1152\/ajpcell.00087.2004","article-title":"RhoA, Rac1, and Cdc42 exert distinct effects on epithelial barrier via selective structural and biochemical modulation of junctional proteins and F-actin","volume":"287","author":"Bruewer","year":"2004","journal-title":"Am J Physiol Cell Physiol"},{"key":"10.1016\/j.aninu.2021.06.016_bib3","doi-asserted-by":"crossref","first-page":"76607","DOI":"10.1039\/C5RA15793E","article-title":"Effects of spermine on the morphology, digestive enzyme activities, and antioxidant status of jejunum in suckling rats","volume":"5","author":"Cao","year":"2015","journal-title":"RSC Adv"},{"key":"10.1016\/j.aninu.2021.06.016_bib4","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1038\/pr.2012.137","article-title":"Arginine and citrulline protect intestinal cell monolayer tight junctions from hypoxia-induced injury in piglets","volume":"72","author":"Chapman","year":"2012","journal-title":"Pediatr Res"},{"key":"10.1016\/j.aninu.2021.06.016_bib5","first-page":"621","article-title":"Oral administration of spermine advances intestinal maturation in sucking piglets","volume":"82","author":"Cheng","year":"2006","journal-title":"J\u00a0Anim Sci"},{"key":"10.1016\/j.aninu.2021.06.016_bib6","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1128\/MCB.06029-11","article-title":"Occludin: one protein, many forms","volume":"32","author":"Cummins","year":"2012","journal-title":"Mol Cell Biol"},{"key":"10.1016\/j.aninu.2021.06.016_bib7","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1111\/j.1749-6632.2012.06526.x","article-title":"Myosin light chain kinase: pulling the strings of epithelial tight junction function","volume":"1258","author":"Cunningham","year":"2012","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.aninu.2021.06.016_bib8","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1113\/expphysiol.2005.031583","article-title":"Intestinal effects of long-lasting spermine ingestion by suckling rats","volume":"90","author":"Deloyer","year":"2005","journal-title":"Exp Physiol"},{"key":"10.1016\/j.aninu.2021.06.016_bib9","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1016\/j.yexcr.2008.11.012","article-title":"Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1","volume":"315","author":"Du","year":"2009","journal-title":"Exp Cell Res"},{"key":"10.1016\/j.aninu.2021.06.016_bib10","first-page":"1619","article-title":"D-lactate in human and ruminant metabolism","volume":"135","author":"Ewaschuk","year":"2005","journal-title":"J\u00a0Nutr"},{"key":"10.1016\/j.aninu.2021.06.016_bib11","doi-asserted-by":"crossref","first-page":"31323","DOI":"10.1039\/C6RA05361K","article-title":"Spermine: new insights into the intestinal development and serum antioxidant status of suckling piglets","volume":"6","author":"Fang","year":"2016","journal-title":"RSC Adv"},{"key":"10.1016\/j.aninu.2021.06.016_bib12","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1046\/j.1440-1746.2003.03032.x","article-title":"Intestinal barrier: an interface between health and disease","volume":"18","author":"Farhadi","year":"2003","journal-title":"J\u00a0Gastroenterol Hepatol"},{"key":"10.1016\/j.aninu.2021.06.016_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1018616814417","article-title":"Myosin light chain kinases","volume":"18","author":"Gallagher","year":"1997","journal-title":"J\u00a0Muscle Res Cell Motil"},{"key":"10.1016\/j.aninu.2021.06.016_bib14","doi-asserted-by":"crossref","first-page":"G1159","DOI":"10.1152\/ajpgi.00407.2004","article-title":"Polyamines are necessary for synthesis and stability of occludin protein in intestinal epithelial cells","volume":"288","author":"Guo","year":"2005","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"10.1016\/j.aninu.2021.06.016_bib15","doi-asserted-by":"crossref","first-page":"10423","DOI":"10.1074\/jbc.M007136200","article-title":"Regulation of tight junction permeability and occludin phosphorylation by Rhoa-p160ROCK-dependent and -independent mechanisms","volume":"276","author":"Hirase","year":"2001","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.aninu.2021.06.016_bib16","doi-asserted-by":"crossref","first-page":"5981","DOI":"10.1074\/jbc.274.9.5981","article-title":"Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and \u03b1 catenin","volume":"274","author":"Itoh","year":"1999","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.aninu.2021.06.016_bib17","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1083\/jcb.142.1.101","article-title":"Structural and functional regulation of tight junctions by RhoA and Rac1 small GTPases","volume":"142","author":"Jou","year":"1998","journal-title":"J\u00a0Cell Biol"},{"key":"10.1016\/j.aninu.2021.06.016_bib18","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1007\/BF02088570","article-title":"Analysis of structural and biochemical events occurring in the small intestine after dietary polyamine ingestion in suckling rats","volume":"41","author":"Kaouass","year":"1996","journal-title":"Dig Dis Sci"},{"key":"10.1016\/j.aninu.2021.06.016_bib19","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/S0016-5085(00)70351-9","article-title":"Claudins regulate the intestinal barrier in response to immune mediators","volume":"118","author":"Kinugasa","year":"2000","journal-title":"Gastroenterology"},{"key":"10.1016\/j.aninu.2021.06.016_bib20","series-title":"Regulation of tight junction barrier function by phospholipase C","author":"Klein","year":"2007"},{"key":"10.1016\/j.aninu.2021.06.016_bib22","doi-asserted-by":"crossref","first-page":"48769","DOI":"10.1039\/C5RA01507C","article-title":"Metabolomic strategy for the detection of metabolic effects of spermine supplementation in weaned rats","volume":"5","author":"Liu","year":"2015","journal-title":"RSC Adv"},{"key":"10.1016\/j.aninu.2021.06.016_bib23","doi-asserted-by":"crossref","first-page":"26709","DOI":"10.1039\/C9RA10406B","article-title":"Effects of spermine on ileal physical barrier, antioxidant capacity, metabolic profile and large intestinal bacteria in piglets","volume":"10","author":"Liu","year":"2020","journal-title":"RSC Adv"},{"key":"10.1016\/j.aninu.2021.06.016_bib24","first-page":"e13167","article-title":"Digestive abilities, amino acid transporter expression, and metabolism in the intestines of piglets fed with spermine","volume":"44","author":"Liu","year":"2020","journal-title":"J\u00a0Food Biochem"},{"key":"10.1016\/j.aninu.2021.06.016_bib25","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.3748\/wjg.v17.i17.2161","article-title":"Wound healing of intestinal epithelial cells","volume":"17","author":"Lizuka","year":"2011","journal-title":"World J Gastroenterol"},{"key":"10.1016\/j.aninu.2021.06.016_bib26","article-title":"STIM1 promotes porcine intestinal epithelial cell restitution by TRPC1 signaling","author":"Mo","year":"2021","journal-title":"Anim Biotechnol"},{"key":"10.1016\/j.aninu.2021.06.016_bib27","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/iub.1237","article-title":"The function of spermine","volume":"66","author":"Pegg","year":"2014","journal-title":"IUBMB Life"},{"key":"10.1016\/j.aninu.2021.06.016_bib28","doi-asserted-by":"crossref","first-page":"7210","DOI":"10.2741\/3223","article-title":"Oxidative stress-induced disruption of epithelial and endothelial tight junctions","volume":"13","author":"Rao","year":"2008","journal-title":"Front Biosci"},{"key":"10.1016\/j.aninu.2021.06.016_bib29","doi-asserted-by":"crossref","first-page":"C1499","DOI":"10.1152\/ajpcell.00232.2008","article-title":"Rac1 promotes intestinal epithelial restitution by increasing Ca2+ influx through interaction with phospholipase C-\u03b31 after wounding","volume":"295","author":"Rao","year":"2008","journal-title":"Am J Physiol Cell Physiol"},{"key":"10.1016\/j.aninu.2021.06.016_bib30","doi-asserted-by":"crossref","first-page":"13039","DOI":"10.1074\/jbc.M208741200","article-title":"The requirement for polyamines for intestinal epithelial cell migration is mediated through Rac1","volume":"278","author":"Ray","year":"2003","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.aninu.2021.06.016_bib31","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/cm.20172","article-title":"MEK\/ERK regulates adherens junctions and migration through Rac1","volume":"64","author":"Ray","year":"2007","journal-title":"Cell Motil Cytoskelet"},{"key":"10.1016\/j.aninu.2021.06.016_bib32","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.it.2011.04.003","article-title":"The intestinal epithelial barrier in the control of homeostasis and immunity","volume":"32","author":"Rescigno","year":"2011","journal-title":"Trends Immunol"},{"key":"10.1016\/j.aninu.2021.06.016_bib33","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jep.2014.10.059","article-title":"Atractylodes macrocephala Koidz stimulates intestinal epithelial cell migration through a polyamine dependent mechanism","volume":"159","author":"Song","year":"2015","journal-title":"J\u00a0Ethnopharmacol"},{"key":"10.1016\/j.aninu.2021.06.016_bib34","first-page":"861","article-title":"Effects of methanol extracts from Atractylodes macrocephalae Rhizoma on small intestinal epithelial cell proliferation and migration, and expression of phospholipase C-\u03b3l","volume":"36","author":"Song","year":"2016","journal-title":"Chin J Integr Tradit West Med"},{"key":"10.1016\/j.aninu.2021.06.016_bib35","doi-asserted-by":"crossref","first-page":"3758","DOI":"10.1182\/blood-2004-03-0781","article-title":"Rac1 is the small GTPase responsible for regulating the neutrophil chemotaxis compass","volume":"104","author":"Sun","year":"2004","journal-title":"Blood"},{"key":"10.1016\/j.aninu.2021.06.016_bib36","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1152\/physiol.00027.2004","article-title":"The molecular physiology of tight junction pores","volume":"19","author":"Van Itallie","year":"2004","journal-title":"Physiology"},{"key":"10.1016\/j.aninu.2021.06.016_bib37","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.ejpb.2014.12.012","article-title":"Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers","volume":"89","author":"Watari","year":"2015","journal-title":"Eur J Pharm Biopharm"},{"key":"10.1016\/j.aninu.2021.06.016_bib38","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.fsi.2014.07.004","article-title":"Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella)","volume":"40","author":"Wen","year":"2014","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.aninu.2021.06.016_bib39","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1017\/S0007114510005660","article-title":"Intestinal barrier function and absorption in pigs after weaning: a review","volume":"105","author":"Wijtten","year":"2011","journal-title":"Br J Nutr"},{"key":"10.1016\/j.aninu.2021.06.016_bib41","doi-asserted-by":"crossref","first-page":"8472","DOI":"10.1093\/nar\/gkr567","article-title":"Chk2-dependent HuR phosphorylation regulates occludin mRNA translation and epithelial barrier function","volume":"39","author":"Yu","year":"2011","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.aninu.2021.06.016_bib42","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1146\/annurev.nutr.23.011702.073036","article-title":"Trophic and cytoprotective nutrition for intestinal adaptation, mucosal repair, and barrier function","volume":"23","author":"Ziegler","year":"2003","journal-title":"Annu Rev Nutr"}],"container-title":["Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S240565452100175X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S240565452100175X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T08:27:04Z","timestamp":1762504024000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S240565452100175X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":40,"alternative-id":["S240565452100175X"],"URL":"https:\/\/doi.org\/10.1016\/j.aninu.2021.06.016","relation":{},"ISSN":["2405-6545"],"issn-type":[{"value":"2405-6545","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Spermine protects intestinal barrier integrity through ras-related C3 botulinum toxin substrate 1\/phospholipase C-\u03b31 signaling pathway in piglets","name":"articletitle","label":"Article Title"},{"value":"Animal Nutrition","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aninu.2021.06.016","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Chinese Association of Animal Science and Veterinary Medicine. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.","name":"copyright","label":"Copyright"}]}}