{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:53:49Z","timestamp":1790654029620,"version":"4.1.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Animal Reproduction Science"],"published-print":{"date-parts":[[2007,5]]},"DOI":"10.1016\/j.anireprosci.2006.04.049","type":"journal-article","created":{"date-parts":[[2006,5,3]],"date-time":"2006-05-03T09:21:07Z","timestamp":1146648067000},"page":"135-144","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":18,"title":["Preservation of tomcat (Felis catus) semen in variable temperatures"],"prefix":"10.1016","volume":"99","author":[{"given":"Marta","family":"Siemieniuch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Dubiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.anireprosci.2006.04.049_bib1","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/S0093-691X(97)00048-4","article-title":"Sperm morphology is better in the second ejaculate than in the first in domestic cats electroejaculated twice during the same period of anesthesia","volume":"47","author":"Axn\u00e9r","year":"1997","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2006.04.049_bib2","unstructured":"Axn\u00e9r, E., 1998. Mating and artificial insemination in domestic cats. In: British Small Animal Veterinary Association Manual of Small Animal Reproduction and Neonatology. BSVA, Cheltenham, UK, pp. 105\u2013110, (chapter 10)."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib3","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1016\/S0093-691X(98)00200-3","article-title":"Morphology of spermatozoa in the cauda epididymides before and after electroejaculation and a comparison with ejaculated spermatozoa in the domestic cat","volume":"50","author":"Axn\u00e9r","year":"1998","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2006.04.049_bib4","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/S0093-691X(99)00170-3","article-title":"Morphology and motility of spermatozoa from different regions of the epididymal duct in the domestic cat","volume":"52","author":"Axn\u00e9r","year":"1999","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2006.04.049_bib5","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.anireprosci.2003.11.003","article-title":"The effect of Equex STM paste and sperm morphology on post-thaw survival of cat epididymal spermatozoa","volume":"84","author":"Axn\u00e9r","year":"2004","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib6","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S1388-1981(99)00110-9","article-title":"Lipid translocation across the plasma membrane of mammalian cells","volume":"1439","author":"Bevers","year":"1999","journal-title":"Acta Biochim. Biophys."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib7","unstructured":"CITES, 1973. Convention on International Trade in Endangered species of Wild Flora and Fauna, part of the Endangered Species Act (PL 93-205, 93rd Congress) and in 50 appendices. Code Fed. Reg., part 23."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib8","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0015-0282(00)01694-0","article-title":"Cryopreservation\u2014thawing of fractionated human spermatozoa and plasma membrane translocation of phosphatidylserine","volume":"75","author":"Duru","year":"2001","journal-title":"Fert. Steril."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib9","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0378-4320(00)00106-8","article-title":"Current state in biotechnology in canine and feline reproduction","volume":"60\u201361","author":"Farstad","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib10","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1095\/biolreprod53.2.276","article-title":"Viability assessment of mammalian sperm using SYBR-14 and propidium iodide","volume":"53","author":"Garner","year":"1995","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib11","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1093\/molehr\/5.2.109","article-title":"Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage","volume":"5","author":"Glander","year":"1999","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib12","first-page":"73","article-title":"Reproductive biology of the domestic cat with special reference to endocrinology, sperm function and in vitro fertilization","volume":"39","author":"Goodrowe","year":"1989","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib13","first-page":"297","article-title":"Comparative cryopreservation and capacitation of spermatozoa from epididymides and vasa deferentia of the domestic cat","volume":"47","author":"Hay","year":"1993","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib14","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1530\/ror.0.0040143","article-title":"Role of reproductive technologies and genetic resource banks in animal conservation","volume":"4","author":"Holt","year":"1999","journal-title":"Rev. Reprod."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib15","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1002\/j.1939-4640.1990.tb03229.x","article-title":"Teratospermic and normospermic domestics cats: ejaculate traits, pituitary-gonadal hormones and improvement of spermatozoal motility and morphology after swim-up processing","volume":"11","author":"Howard","year":"1990","journal-title":"J. Androl."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib16","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0165-0378(01)00101-2","article-title":"Interactions between zona pellucida glycoproteins and sperm proacrosin\/acrosin during fertilization","volume":"53","author":"Howes","year":"2002","journal-title":"J. Reprod. Immunol."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib17","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/S0093-691X(99)00220-4","article-title":"Sex preselection: high-speed flow cytometric sorting of X and Y sperm for maximum efficiency","volume":"52","author":"Johnson","year":"1999","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2006.04.049_bib18","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0378-4320(00)00088-9","article-title":"Sexing mammalian sperm for production of offspring: the state-of-the-art","volume":"60\u201361","author":"Johnson","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1046\/j.1365-2605.1999.00151.x","article-title":"To store or mature spermatozoa? The primary role of the epididymis","volume":"22","author":"Jones","year":"1999","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib20","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/0378-4320(94)90044-2","article-title":"In vitro fertilization of follicular oocytes of domestic cat using fresh and cryopreserved epididymal spermatozoa","volume":"35","author":"Lengwinat","year":"1994","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib21","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S1098-612X(03)00029-9","article-title":"Conservation of feline semen. Part I. Cooling and freezing protocols","volume":"5","author":"Luvoni","year":"2003","journal-title":"J. Feline Med. Surg."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib22","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S1098-612X(03)00030-5","article-title":"Conservation of feline semen. Part II. Cold-induced damages on spermatozoal fertilizing ability","volume":"5","author":"Luvoni","year":"2003","journal-title":"J. Feline Med. Surg."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1242\/jcs.112.1.11","article-title":"Stability of transbilayer phospholipid asymmetry in viable ram sperm cells after cryotreatment","volume":"112","author":"M\u00fcller","year":"1999","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib24","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1016\/S0093-691X(02)01231-1","article-title":"Membrane changes during different stages of a freeze\u2013thaw protocol for equine semen cryopreservation","volume":"59","author":"Neidl","year":"2003","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2006.04.049_bib25","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0168-9525(86)90204-0","article-title":"Molecular genetics in the domestic cat and its relatives","volume":"2","author":"O\u2019Brien","year":"1986","journal-title":"Trends Genet."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib26","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/S0093-691X(03)00081-5","article-title":"Assessment of fresh and frozen\u2013thawed boar semen using an Annexin-V assay: a new method of evaluating sperm membrane integrity","volume":"60","author":"Pe\u00f1a","year":"2003","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2006.04.049_bib27","first-page":"1053","article-title":"Semen collection, freezing and insemination in the domestic cat","author":"Platz","year":"1976","journal-title":"Proc. Eight Int. Congr. Anim. Reprod. Art. Insemin. Cracow."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib28","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1530\/jrf.0.0520279","article-title":"Pregnancy in the domestic cat after artificial insemination with previously frozen semen","volume":"52","author":"Platz","year":"1978","journal-title":"J. Reprod. Fertil."},{"issue":"Suppl. 1","key":"10.1016\/j.anireprosci.2006.04.049_bib29","first-page":"117","article-title":"Semen storage in the domestic felid: a comparison of cryopreservation methods and storage temperature.","volume":"44","author":"Pope","year":"1991","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib30","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0093-691X(99)00249-6","article-title":"Embryo technology in conservation efforts for endangered felids","volume":"53","author":"Pope","year":"2000","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2006.04.049_bib31","first-page":"423","article-title":"The phenomenon and significance of teratospermia in felids","volume":"57","author":"Pukazhenthi","year":"2001","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib32","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/S0011-2240(02)00035-4","article-title":"Osmotic properties of spermatozoa from felids producing different proportions of pleiomorphisms: influence of adding and removing cryoprotectant","volume":"44","author":"Pukazhenthi","year":"2002","journal-title":"Cryobiology"},{"key":"10.1016\/j.anireprosci.2006.04.049_bib33","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1016\/S0093-691X(97)00066-6","article-title":"Effects of STM Paste on viability of frozen thawed dog spermatozoa during in vitro incubation at 38\u00b0C","volume":"47","author":"Rota","year":"1997","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2006.04.049_bib34","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0378-4320(00)00073-7","article-title":"The use of transmigration and Spermac\u2122 stain to evaluate epididymal cat spermatozoa","volume":"59","author":"Sch\u00e4fer","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib35","first-page":"198","article-title":"Artificial insemination in the cat (Felis catus L.)","volume":"20","author":"Sojka","year":"1970","journal-title":"Lab. Anim. Care"},{"key":"10.1016\/j.anireprosci.2006.04.049_bib36","first-page":"1241","article-title":"Unilateral intrauterine horn insemination of fresh semen in cats","volume":"62","author":"Tsutsui","year":"2000","journal-title":"Jpn. J. Vet. Sci."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib37","first-page":"347","article-title":"Intratubal insemination with fresh semen in cats","volume":"57","author":"Tsutsui","year":"2001","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib38","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1136\/vr.97.1.12","article-title":"Use of Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa","volume":"97","author":"Watson","year":"1975","journal-title":"Vet. Rec."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib39","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1095\/biolreprod34.5.937","article-title":"Reproductive physiology of the clouded leopard. I. Electroejaculates contain high proportions of pleiomorphic spermatozoa throughout the year","volume":"34","author":"Wildt","year":"1986","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.anireprosci.2006.04.049_bib40","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/0093-691X(96)00209-9","article-title":"Comparison of the fertility of cryopreserved stallion spermatozoa with sperm motion analyses, flow cytometric evaluation, and zona-free hamster oocyte penetration","volume":"46","author":"Wilhelm","year":"1996","journal-title":"Theriogenology"}],"container-title":["Animal Reproduction Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432006002314?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432006002314?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T06:07:40Z","timestamp":1759903660000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378432006002314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5]]},"references-count":40,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2007,5]]}},"alternative-id":["S0378432006002314"],"URL":"https:\/\/doi.org\/10.1016\/j.anireprosci.2006.04.049","relation":{},"ISSN":["0378-4320"],"issn-type":[{"value":"0378-4320","type":"print"}],"subject":[],"published":{"date-parts":[[2007,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Preservation of tomcat (Felis catus) semen in variable temperatures","name":"articletitle","label":"Article Title"},{"value":"Animal Reproduction Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.anireprosci.2006.04.049","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2006 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}