{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:14:26Z","timestamp":1767852866883,"version":"3.49.0"},"reference-count":37,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Animal Reproduction Science"],"published-print":{"date-parts":[[2010,3]]},"DOI":"10.1016\/j.anireprosci.2009.06.004","type":"journal-article","created":{"date-parts":[[2009,6,19]],"date-time":"2009-06-19T04:34:18Z","timestamp":1245386058000},"page":"7-12","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"title":["Pregnancy loss in heifers after artificial insemination with frozen-thawed, sex-sorted, re-frozen-thawed dairy bull sperm"],"prefix":"10.1016","volume":"118","author":[{"given":"S.L.","family":"Underwood","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Bathgate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ebsworth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.M.C.","family":"Maxwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.anireprosci.2009.06.004_bib1","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.anireprosci.2007.06.028","article-title":"Early pregnancy loss in sows after low dose, deep uterine artificial insemination with sex-sorted, frozen-thawed sperm","volume":"104","author":"Bathgate","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib2","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1016\/j.theriogenology.2005.04.011","article-title":"Fertility in heifers and cows after low dose insemination with sex-sorted and non-sorted sperm under field conditions","volume":"64","author":"Bodmer","year":"2005","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib3","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.theriogenology.2008.09.026","article-title":"field data with sexed semen","volume":"71","author":"Borchersen","year":"2009","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib4","first-page":"369","article-title":"Pregnancy rates and first born calves by artificial insemination using sexed sperm in Argentina","volume":"57","author":"Brogliatti","year":"2002","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib5","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.theriogenology.2006.08.005","article-title":"Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa","volume":"67","author":"de Graaf","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib6","first-page":"99","article-title":"Artificial insemination of lactating Angus cows with sexed semen","volume":"77","author":"Doyle","year":"1999","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib7","series-title":"Salamon's Artificial Insemination of Sheep and Goats","author":"Evans","year":"1987"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib8","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1016\/0093-691X(95)00063-E","article-title":"The frequency of endemic fetal loss in dairy cattle: a review","volume":"43","author":"Forar","year":"1995","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib9","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.theriogenology.2008.09.025","article-title":"What affects fertility more, low sperm dosage or the sorting process?","volume":"71","author":"Frijters","year":"2009","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib10","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/j.theriogenology.2005.09.009","article-title":"Flow cytometric sexing of mammalian sperm","volume":"65","author":"Garner","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib11","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1071\/R96046","article-title":"Capacitation status and fertility of fresh and frozen-thawed ram spermatozoa","volume":"9","author":"Gillan","year":"1997","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib12","series-title":"Reproduction in Farm Animals","year":"2000"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib13","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1071\/RD03060","article-title":"In vitro and in vivo assessment of functional capacity of flow cytometrically sorted ram spermatozoa after freezing and thawing","volume":"15","author":"Hollinshead","year":"2003","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib14","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1530\/rep.1.00049","article-title":"Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen-thawed sex- and re-frozen-thawed ram spermatozoa","volume":"127","author":"Hollinshead","year":"2004","journal-title":"Reproduction"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib15","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/j.theriogenology.2003.12.030","article-title":"Assessment of in vitro sperm characteristics after flow cytometric sorting of frozen-thawed bull spermatozoa","volume":"62","author":"Hollinshead","year":"2004","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib16","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1111\/j.1439-0531.1991.tb01546.x","article-title":"Sex preselection in swine: Altered sex ratios in offspring following surgical insemination of flow sorted X- and Y-bearing sperm","volume":"26","author":"Johnson","year":"1991","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib17","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1439-0531.2006.00730.x","article-title":"Reduction of oxidative stress in bovine spermatozoa during flow cytometric sorting","volume":"42","author":"Klinc","year":"2007","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib18","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1016\/S0093-691X(01)00715-4","article-title":"Factors affecting pregnancy loss from gestation Day 38 to 90 in lactating dairy cows from a single herd","volume":"57","author":"L\u00f3pez-Gatius","year":"2002","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib19","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1016\/S0093-691X(99)00225-3","article-title":"In vitro fertilization with flow-cytometrically-sorted bovine sperm","volume":"52","author":"Lu","year":"1999","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib20","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0167-5877(96)01142-7","article-title":"Epidemiology of bovine abortions in Israeli dairy herds","volume":"31","author":"Markusfeld-Nir","year":"1997","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib21","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1002\/mrd.20573","article-title":"Altered mRNA expression patterns in bovine blastocysts after fertilisation in vitro using flow-cytometrically sex-sorted sperm","volume":"74","author":"Morton","year":"2007","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib22","doi-asserted-by":"crossref","first-page":"693","DOI":"10.2527\/jas1981.533693x","article-title":"Effects of thawing temperature, number of spermatozoa and spermatozoal quality on fertility of bovine spermatozoa packaged in 0.5 French straws","volume":"53","author":"Pace","year":"1981","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib23","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1530\/rep.1.01173","article-title":"In vitro fertilisation with frozen-thawed bovine sperm sexed by flow cytometry and validated for accuracy by real-time PCR","volume":"132","author":"Puglisi","year":"2006","journal-title":"Reproduction"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib24","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1016\/S0093-691X(99)00224-1","article-title":"Cryopreservation of flow-sorted bovine spermatozoa","volume":"52","author":"Schenk","year":"1999","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib25","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/S0093-691X(97)00368-3","article-title":"Uterine horn insemination of heifers with very low numbers of nonfrozen and sexed spermatozoa","volume":"48","author":"Seidel","year":"1997","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib26","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1530\/rep.0.1240733","article-title":"Current status of sexing mammalian spermatozoa","volume":"124","author":"Seidel","year":"2002","journal-title":"Reproduction"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib27","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1016\/S0093-691X(99)00226-5","article-title":"Insemination of heifers with sexed sperm","volume":"52","author":"Seidel","year":"1999","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib28","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.2527\/1998.7671896x","article-title":"Improving the resolution of cryopreserved X- and Y-sperm during DNA flow cytometric analysis with the addition of Percoll to quench the fluorescence of dead sperm","volume":"76","author":"Stap","year":"1998","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib29","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.anireprosci.2003.12.009","article-title":"Factors affecting retention of early pregnancy in dairy cattle","volume":"84","author":"Starbuck","year":"2004","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib30","first-page":"245","article-title":"Bull fertility as affected by an interaction between motile spermatozoa concentration and fertility level in artificial insemination","author":"Sullivan","year":"1968","journal-title":"VI Ed. Congr. Reprod. Insem. Artif. (Paris)"},{"key":"10.1016\/j.anireprosci.2009.06.004_bib31","unstructured":"Trimberger, G.W., 1948. Breeding efficiency in dairy cattle from artificial insemination at various intervals before and after ovulation. Bull. No. 153. Nebraska Agric. Exp. Stn. Lincoln."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib32","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.2527\/2004.8241029x","article-title":"Characteristics of calves produced with sperm sexed by flow cytometry\/cell sorting","volume":"82","author":"Tubman","year":"2004","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib33","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1111\/j.1439-0531.2008.01127.x","article-title":"Development of procedures for sex-sorting frozen-thawed bovine spermatozoa","volume":"44","author":"Underwood","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib34","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1071\/RD9950871","article-title":"Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function","volume":"7","author":"Watson","year":"1995","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib35","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S0378-4320(00)00099-3","article-title":"Cause of reduced fertility with cryopreserved semen","volume":"60-61","author":"Watson","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib36","doi-asserted-by":"crossref","first-page":"E120","DOI":"10.3168\/jds.S0022-0302(04)70067-3","article-title":"Exploring the role of sexed semen in dairy production systems","volume":"87","author":"Weigel","year":"2004","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.anireprosci.2009.06.004_bib37","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1016\/S0093-691X(99)00221-6","article-title":"Sex preselection: laboratory validation of the sperm sex ratio of flow sorted X- and Y- sperm by sort reanalysis for DNA","volume":"52","author":"Welch","year":"1999","journal-title":"Theriogenology"}],"container-title":["Animal Reproduction Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432009001559?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432009001559?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T06:15:26Z","timestamp":1759904126000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378432009001559"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["S0378432009001559"],"URL":"https:\/\/doi.org\/10.1016\/j.anireprosci.2009.06.004","relation":{},"ISSN":["0378-4320"],"issn-type":[{"value":"0378-4320","type":"print"}],"subject":[],"published":{"date-parts":[[2010,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Pregnancy loss in heifers after artificial insemination with frozen-thawed, sex-sorted, re-frozen-thawed dairy bull sperm","name":"articletitle","label":"Article Title"},{"value":"Animal Reproduction Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.anireprosci.2009.06.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2009 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}