{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:33:11Z","timestamp":1786984391476,"version":"build-2736575974"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Chief Research Officer, Buffalo Research Institute, Pattoki"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Animal Reproduction Science"],"published-print":{"date-parts":[[2016,4]]},"DOI":"10.1016\/j.anireprosci.2016.02.010","type":"journal-article","created":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T13:00:36Z","timestamp":1455195636000},"page":"83-88","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":35,"special_numbering":"C","title":["Royal jelly supplementation in semen extender enhances post-thaw quality and fertility of Nili-Ravi buffalo bull sperm"],"prefix":"10.1016","volume":"167","author":[{"given":"Qaisar","family":"Shahzad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Usman","family":"Mehmood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamayun","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma ul","family":"Husna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saima","family":"Qadeer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asima","family":"Azam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahid","family":"Naseer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ejaz","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Safdar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mushtaq","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.anireprosci.2016.02.010_bib0005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.6000\/1927-520X.2012.01.01.01","article-title":"Effect of royal jelly on the fertilizing ability of buffalo spermatozoa in vitro","volume":"1","author":"Abd-Allah","year":"2012","journal-title":"J. Buffalo Sci."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0010","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1111\/j.1439-0531.2008.01240.x","article-title":"Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa","volume":"44","author":"Andrabi","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0015","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.anireprosci.2007.07.003","article-title":"Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa","volume":"104","author":"Andrabi","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0020","first-page":"41","article-title":"Effect of l-cysteine addition in tris-citric egg yolk extender on post-thaw quality of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa","volume":"43","author":"Ansari","year":"2011","journal-title":"Pak. J. Zool."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0025","first-page":"106","article-title":"Factors affecting the efficiency of artificial insemination in cattle and buffalo in Punjab, Pakistan","volume":"23","author":"Anzar","year":"2003","journal-title":"Pak. Vet. J."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0030","first-page":"67","article-title":"Chemical composition and antimicrobial activity of royal jelly","volume":"44","author":"B\u0103rnu\u0163iu","year":"2011","journal-title":"Anim. Sci. Biotechnol."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0035","first-page":"686137","article-title":"Impacts of oxidative stress and antioxidants on semen functions","author":"Bansal","year":"2011","journal-title":"Vet. Med. Int."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0040","doi-asserted-by":"crossref","first-page":"139","DOI":"10.6000\/1927-520X.2012.01.02.02","article-title":"Technologies related with the artificial insemination in buffalo","volume":"1","author":"Barile","year":"2012","journal-title":"J. Buffalo Sci."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0045","doi-asserted-by":"crossref","first-page":"228","DOI":"10.4161\/oxim.3.4.12858","article-title":"Exogenous antioxidants-double-edged swords in cellular redox state: health beneficial effects at physiologic doses versus deleterious effects at high doses","volume":"3","author":"Bouayed","year":"2010","journal-title":"Oxid. Med. Cell. Longev."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0050","first-page":"46","article-title":"The protective effect of antioxidants on liquid and frozen stored ram semen-review","volume":"47","author":"Budai","year":"2014","journal-title":"Anim. Sci. Biotechnol."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0055","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.anireprosci.2010.05.008","article-title":"Royal jelly counteracts bucks\u2019 summer infertility","volume":"121","author":"Elnagar","year":"2010","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0060","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1254\/jjp.53.331","article-title":"Augmentation of wound healing by royal jelly (RJ) in streptozotocin-diabetic rats","volume":"53","author":"Fujii","year":"1990","journal-title":"Jpn. J. Pharmacol."},{"issue":"1","key":"10.1016\/j.anireprosci.2016.02.010_bib0065","first-page":"113","article-title":"Study on the effect of royal jelly on reproductive parameters in streptozotocin-induced diabetic rats","volume":"9","author":"Ghanbari","year":"2015","journal-title":"Int. J. Fertil. Steril."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0070","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.2527\/jas.2005-766","article-title":"Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in percoll-treated viable boar sperm using fluorescence-activated flow cytometry","volume":"84","author":"Guthrie","year":"2006","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0075","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1080\/00218839.1985.11100649","article-title":"Composition of freshly harvested and commercial royal jelly","volume":"24","author":"Howe","year":"1985","journal-title":"J. Apic. Res."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0080","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.anireprosci.2010.04.180","article-title":"The cryoprotective effects of ascorbic acid supplementation on bovine semen quality","volume":"121","author":"Hu","year":"2010","journal-title":"Anim. Sci. Rep."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0085","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1016\/j.theriogenology.2008.12.023","article-title":"Butylated hydroxytoluene inclusion in semen extender improves the post-thawed semen quality of Nili-Ravi buffalo (Bubalus bubalis)","volume":"71","author":"Ijaz","year":"2009","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0090","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3177\/jnsv.47.394","article-title":"Antifatigue effect of fresh royal jelly in mice","volume":"47","author":"Kamakura","year":"2001","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0095","first-page":"981793","article-title":"Royal jelly modulates oxidative stress and apoptosis in liver and kidneys of rats treated with cisplatin","author":"Karadeniz","year":"2011","journal-title":"Oxid. Med. Cell. Longev."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0100","doi-asserted-by":"crossref","first-page":"318","DOI":"10.5713\/ajas.2010.90387","article-title":"The effects of different concentrations of glycine and cysteine on the freezability of Moghani ram spermatozoa","volume":"23","author":"Khalili","year":"2010","journal-title":"Asian Aust. J. Anim. Sci."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0105","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1248\/cpb.55.1528","article-title":"Compositions of royal jelly II: organic acid glycosides and sterols of the royal jelly of honeybees (Apis mellifera)","volume":"55","author":"Kodai","year":"2007","journal-title":"Chem. Pharm. Bull."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0110","doi-asserted-by":"crossref","first-page":"119","DOI":"10.3109\/10520299209110020","article-title":"Viability and acrosome staining of bull: boar and rabbit spermatozoa","volume":"67","author":"Kovacs","year":"1992","journal-title":"Biotechnol. Histochem."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0115","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/S0955-2863(01)00198-X","article-title":"Gastrointestinal enzyme production of bioactive peptides from royal jelly protein and their antihypertensive ability in SHR","volume":"13","author":"Matsui","year":"2002","journal-title":"J. Nutr. Biochem."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0120","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1007\/BF00493438","article-title":"Induced metachromasia in bull spermatozoa","volume":"74","author":"Mello","year":"1982","journal-title":"Histochemistry"},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0125","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.smallrumres.2013.03.003","article-title":"Royal jelly improves the sperm parameters of ram semen during liquid storage and serves as an antioxidant source","volume":"113","author":"Moradi","year":"2013","journal-title":"Small Rumin. Res."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0130","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.anireprosci.2005.11.002","article-title":"A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature","volume":"96","author":"Nair","year":"2006","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0135","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/165348","article-title":"Effect of addition of taurine on the liquid storage (5\u00b0C) of Mithun (Bos frontalis) semen","author":"Perumal","year":"2013","journal-title":"Vet. Med. Int."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0140","first-page":"89","article-title":"Scavenging of reactive oxygen species as the mechanism of drug action","volume":"47","author":"Robak","year":"1994","journal-title":"Pol. J. Pharmacol."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0145","first-page":"81","article-title":"Effect of antioxidants added to boar semen extender on the semen survival time and sperm chromatin structure","volume":"3","author":"Szcze\u015bniak-Fabia\u0144czyk","year":"2003","journal-title":"Reprod. Biol."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0150","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1016\/j.foodchem.2008.11.058","article-title":"Estimation and characterisation of major royal jelly proteins obtained from the honeybee Apis merifera","volume":"114","author":"Tamura","year":"2009","journal-title":"Food Chem."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0155","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1042\/bj0341155","article-title":"The chemical nature of royal jelly","volume":"34","author":"Townsend","year":"1940","journal-title":"Biochem. J."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0160","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1071\/RD9950871","article-title":"Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function","volume":"7","author":"Watson","year":"1995","journal-title":"Reprod. Fert. Dev."},{"key":"10.1016\/j.anireprosci.2016.02.010_bib0165","first-page":"43","article-title":"Protective effect of royal jelly on the sperm parameters and testosterone level and lipid peroxidation in adult mice treated with oxymetholone","volume":"4","author":"Zahmatkesh","year":"2014","journal-title":"Avicenna J. Phytomed."}],"container-title":["Animal Reproduction Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432016300501?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432016300501?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T06:32:50Z","timestamp":1759905170000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378432016300501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4]]},"references-count":33,"alternative-id":["S0378432016300501"],"URL":"https:\/\/doi.org\/10.1016\/j.anireprosci.2016.02.010","relation":{},"ISSN":["0378-4320"],"issn-type":[{"value":"0378-4320","type":"print"}],"subject":[],"published":{"date-parts":[[2016,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Royal jelly supplementation in semen extender enhances post-thaw quality and fertility of Nili-Ravi buffalo bull sperm","name":"articletitle","label":"Article Title"},{"value":"Animal Reproduction Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.anireprosci.2016.02.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}