{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:32:12Z","timestamp":1792801932964,"version":"4.5.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003967","name":"Iran's National Elites Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003967","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Animal Reproduction Science"],"published-print":{"date-parts":[[2021,10]]},"DOI":"10.1016\/j.anireprosci.2021.106836","type":"journal-article","created":{"date-parts":[[2021,8,29]],"date-time":"2021-08-29T09:34:30Z","timestamp":1630229670000},"page":"106836","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"special_numbering":"C","title":["Fertility and flow cytometry evaluations of ram frozen semen in plant-based extender supplemented with Mito-TEMPO"],"prefix":"10.1016","volume":"233","author":[{"given":"Nader","family":"Asadzadeh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Abdollahi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeid","family":"Esmaeilkhanian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Masoudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.anireprosci.2021.106836_bib1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.4103\/1008-682X.122203","article-title":"Oxidative stress and male reproductive health","volume":"16","author":"Aitken","year":"2014","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.anireprosci.2021.106836_bib2","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1095\/biolreprod66.2.354","article-title":"Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility","volume":"66","author":"Anzar","year":"2002","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.anireprosci.2021.106836_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/j.1939-4640.2000.tb03268.x","article-title":"Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon minireview","volume":"21","author":"Bailey","year":"2000","journal-title":"J. Androl."},{"key":"10.1016\/j.anireprosci.2021.106836_bib4","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1093\/toxsci\/kfl077","article-title":"Nuclear translocation of endonuclease G and apoptosis-inducing factor during acetaminophen-induced liver cell injury","volume":"94","author":"Bajt","year":"2006","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.anireprosci.2021.106836_bib5","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1124\/jpet.107.129445","article-title":"Mitochondrial Bax translocation accelerates DNA fragmentation and cell necrosis in a murine model of acetaminophen hepatotoxicity","volume":"324","author":"Bajt","year":"2008","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.anireprosci.2021.106836_bib6","doi-asserted-by":"crossref","first-page":"245","DOI":"10.3109\/19396368.2014.897773","article-title":"Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function","volume":"60","author":"Bateni","year":"2014","journal-title":"Syst. Biol. Reprod. Med."},{"key":"10.1016\/j.anireprosci.2021.106836_bib7","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.smallrumres.2008.11.011","article-title":"Effect of antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora goat semen following cryopreservation","volume":"81","author":"Bucak","year":"2009","journal-title":"Small Rumin. Res."},{"key":"10.1016\/j.anireprosci.2021.106836_bib8","doi-asserted-by":"crossref","first-page":"3003","DOI":"10.4238\/2013.August.20.2","article-title":"Temporal variations of hsp60 and hsf-1 in primary rat myocardial cells in vitro under heat stress","volume":"12","author":"Buriro","year":"2013","journal-title":"Genet. Mol. Res."},{"key":"10.1016\/j.anireprosci.2021.106836_bib9","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.theriogenology.2006.02.022","article-title":"Effect of docosahexaenoic acid and \u03b1-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation","volume":"66","author":"Cerolini","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2021.106836_bib10","doi-asserted-by":"crossref","first-page":"26185","DOI":"10.1074\/jbc.M503062200","article-title":"Disruption of fusion results in mitochondrial heterogeneity and dysfunction","volume":"280","author":"Chen","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.anireprosci.2021.106836_bib11","doi-asserted-by":"crossref","first-page":"90427","DOI":"10.1172\/jci.insight.90427","article-title":"Amphetamines promote mitochondrial dysfunction and DNA damage in pulmonary hypertension","volume":"2","author":"Chen","year":"2017","journal-title":"JCI Insight"},{"key":"10.1016\/j.anireprosci.2021.106836_bib12","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.theriogenology.2005.05.005","article-title":"Genetic sperm defects","volume":"64","author":"Chenoweth","year":"2005","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2021.106836_bib13","doi-asserted-by":"crossref","first-page":"2837","DOI":"10.1089\/ars.2010.3713","article-title":"Lipopolysaccharide-induced mitochondrial DNA depletion","volume":"15","author":"Choumar","year":"2011","journal-title":"Antioxid. Redox Signal."},{"key":"10.1016\/j.anireprosci.2021.106836_bib14","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1161\/CIRCRESAHA.109.214601","article-title":"Therapeutic targeting of mitochondrial superoxide in hypertension","volume":"107","author":"Dikalova","year":"2010","journal-title":"Circ. Res."},{"key":"10.1016\/j.anireprosci.2021.106836_bib15","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1007\/s00204-016-1692-0","article-title":"Mitochondria-targeted antioxidant Mito-Tempo protects against acetaminophen hepatotoxicity","volume":"91","author":"Du","year":"2017","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.anireprosci.2021.106836_bib16","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/0076-6879(90)86134-H","article-title":"Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal","volume":"186","author":"Esterbauer","year":"1990","journal-title":"Methods Enzym."},{"key":"10.1016\/j.anireprosci.2021.106836_bib17","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.taap.2005.03.021","article-title":"Environmental toxicants cause sperm DNA fragmentation as detected by the Sperm Chromatin Structure Assay (SCSA\u00ae)","volume":"207","author":"Evenson","year":"2005","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.anireprosci.2021.106836_bib18","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.cryobiol.2014.09.005","article-title":"Inhibition of ROS production through mitochondria-targeted antioxidant and mitochondrial uncoupling increases post-thaw sperm viability in yellow catfish","volume":"69","author":"Fang","year":"2014","journal-title":"Cryobiology"},{"key":"10.1016\/j.anireprosci.2021.106836_bib19","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.theriogenology.2009.10.005","article-title":"In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen","volume":"73","author":"Forouzanfar","year":"2010","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2021.106836_bib20","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.cryobiol.2017.06.008","article-title":"Effectiveness of human spermatozoa biomarkers as indicators of structural damage during cryopreservation","volume":"78","author":"G\u00f3mez-Torres","year":"2017","journal-title":"Cryobiology"},{"key":"10.1016\/j.anireprosci.2021.106836_bib21","doi-asserted-by":"crossref","first-page":"36994","DOI":"10.1038\/srep36994","article-title":"The sensitivity of the DNA damage checkpoint prevents oocyte maturation in endometriosis","volume":"6","author":"Hamdan","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.anireprosci.2021.106836_bib22","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1111\/andr.12030","article-title":"Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients","volume":"3","author":"Hosseinifar","year":"2015","journal-title":"Andrology"},{"key":"10.1016\/j.anireprosci.2021.106836_bib23","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.cryobiol.2015.04.006","article-title":"Enolase1 (ENO1) and glucose-6-phosphate isomerase (GPI) are good markers to predict human sperm freezability","volume":"71","author":"Jiang","year":"2015","journal-title":"Cryobiology"},{"key":"10.1016\/j.anireprosci.2021.106836_bib24","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1439-0531.2011.01818.x","article-title":"Effect of cryopreservation on apoptotic-like events and its relationship with cryocapacitation of buffalo (Bubalus bubalis) sperm","volume":"47","author":"Kadirvel","year":"2012","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.anireprosci.2021.106836_bib25","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1002\/cyto.a.20623","article-title":"MIFlowCyt: The minimum information about a flow cytometry experiment","volume":"73","author":"Lee","year":"2008","journal-title":"Cytom. Part A"},{"key":"10.1016\/j.anireprosci.2021.106836_bib26","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1016\/j.freeradbiomed.2010.08.018","article-title":"SOD1 and MitoTEMPO partially prevent mitochondrial permeability transition pore opening, necrosis, and mitochondrial apoptosis after ATP depletion recovery","volume":"49","author":"Liang","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.anireprosci.2021.106836_bib27","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.cryobiol.2017.12.009","article-title":"Mitochondria-targeted antioxidant MitoTEMPO improves the post-thaw sperm quality","volume":"80","author":"Lu","year":"2018","journal-title":"Cryobiology"},{"key":"10.1016\/j.anireprosci.2021.106836_bib28","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.cryobiol.2019.10.005","article-title":"Supplementation of chilling storage medium with glutathione protects rooster sperm quality","volume":"92","author":"Masoudi","year":"2020","journal-title":"Cryobiology"},{"key":"10.1016\/j.anireprosci.2021.106836_bib29","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.cryobiol.2019.03.003","article-title":"Improvement of rooster semen quality using coenzyme Q10 during cooling storage in the Lake extender","volume":"88","author":"Masoudi","year":"2019","journal-title":"Cryobiology"},{"key":"10.1016\/j.anireprosci.2021.106836_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.anireprosci.2019.06.015","article-title":"Effects of CoQ10 on the quality of ram sperm during cryopreservation in plant and animal based extenders","volume":"208","author":"Masoudi","year":"2019","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2021.106836_bib31","first-page":"2803","article-title":"Effect of estradiol and oxytocin on ovine cervical relaxation","volume":"11","author":"Masoudi","year":"2012","journal-title":"Afr. J. Biotechnol."},{"key":"10.1016\/j.anireprosci.2021.106836_bib32","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.neulet.2014.10.016","article-title":"Mitochondrial ROS govern the LPS-induced pro-inflammatory response in microglia cells by regulating MAPK and NF-\u03baB pathways","volume":"584","author":"Park","year":"2015","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.anireprosci.2021.106836_bib33","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1161\/ATVBAHA.113.301591","article-title":"Mitochondrial oxidative stress corrupts coronary collateral growth by activating adenosine monophosphate activated kinase-\u03b1 signaling","volume":"33","author":"Pung","year":"2013","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.anireprosci.2021.106836_bib34","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0378-4320(94)90055-8","article-title":"An osmotic resistance test for bovine semen","volume":"36","author":"Revell","year":"1994","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2021.106836_bib35","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0070-2153(06)77001-6","article-title":"The role of the mitochondrion in sperm function: is there a place for oxidative phosphorylation or is this a purely glycolytic process?","volume":"77","author":"Ruiz-Pesini","year":"2007","journal-title":"Curr. Top. Dev. Biol."},{"key":"10.1016\/j.anireprosci.2021.106836_bib36","unstructured":"Scha\u00e4fer, S., Holzmann, A., 2000. The use of transmigration and SpermacTMstain to."},{"key":"10.1016\/j.anireprosci.2021.106836_bib37","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s10561-014-9458-5","article-title":"Supplementation of soybean lecithin-based semen extender by antioxidants: complementary flowcytometric study on post-thawed ram spermatozoa","volume":"16","author":"Sharafi","year":"2015","journal-title":"Cell Tissue Bank"},{"key":"10.1016\/j.anireprosci.2021.106836_bib38","doi-asserted-by":"crossref","unstructured":"Sharma, R., Roychoudhury, S., Singh, N., Sarda, Y., 2017. Methods to measure reactive oxygen species (ROS) and total antioxidant capacity (TAC) in the reproductive system. In: OxidativeStress in Human Reproduction: Shedding Light on a Complicated Phenomenon, 17\u201346.","DOI":"10.1007\/978-3-319-48427-3_2"},{"key":"10.1016\/j.anireprosci.2021.106836_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1262\/jrd.11-138N","article-title":"Oxidative stress and redox regulation on in vitro development of mammalian embryos","volume":"58","author":"Takahashi","year":"2012","journal-title":"J. Reprod. Dev."},{"key":"10.1016\/j.anireprosci.2021.106836_bib40","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1530\/REP-09-0094","article-title":"Expression and putative function of fibronectin and its receptor (integrin \u03b1 5\u03b2 1) in male and female gametes during bovine fertilization in vitro","volume":"138","author":"Thys","year":"2009","journal-title":"Reproduction"},{"key":"10.1016\/j.anireprosci.2021.106836_bib41","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1016\/j.freeradbiomed.2007.12.036","article-title":"A mitochondria-targeted nitroxide is reduced to its hydroxylamine by ubiquinol in mitochondria","volume":"44","author":"Trnka","year":"2008","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.anireprosci.2021.106836_bib42","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1016\/j.bbadis.2013.10.015","article-title":"Oxidative stress and mitochondrial dysfunction in Alzheimer\u2019s disease","volume":"1842","author":"Wang","year":"2014","journal-title":"Biochim. Biophys. Acta Mol. Basis Dis."},{"key":"10.1016\/j.anireprosci.2021.106836_bib43","first-page":"1","article-title":"Mito-TEMPO improves development competence by reducing superoxide in preimplantation porcine embryos","volume":"8","author":"Yang","year":"2018","journal-title":"Sci. Rep."}],"container-title":["Animal Reproduction Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432021001512?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432021001512?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T06:45:49Z","timestamp":1759905949000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378432021001512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10]]},"references-count":43,"alternative-id":["S0378432021001512"],"URL":"https:\/\/doi.org\/10.1016\/j.anireprosci.2021.106836","relation":{},"ISSN":["0378-4320"],"issn-type":[{"value":"0378-4320","type":"print"}],"subject":[],"published":{"date-parts":[[2021,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Fertility and flow cytometry evaluations of ram frozen semen in plant-based extender supplemented with Mito-TEMPO","name":"articletitle","label":"Article Title"},{"value":"Animal Reproduction Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.anireprosci.2021.106836","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"106836"}}